scholarly journals Selenium and tellurium-based nanoparticles as interfering factors in quorum sensing-regulated processes: violacein production and bacterial biofilm formation

Metallomics ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1104-1114 ◽  
Author(s):  
Beatriz Gómez-Gómez ◽  
Lucia Arregui ◽  
Susana Serrano ◽  
Antonio Santos ◽  
Teresa Pérez-Corona ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Persistent Infections ◽  
Violacein Production ◽  
New Strategies

The effect of SeNPs and TeNPs on different processes regulated by QS such as violacein production and biofilm formation is presented. The data open new strategies for controlling persistent infections.

scholarly journals Control of Biofilm Formation in Healthcare: Recent Advances Exploiting Quorum-Sensing Interference Strategies and Multidrug Efflux Pump Inhibitors

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1676 ◽  
Author(s):  
Bindu Subhadra ◽  
Dong Kim ◽  
Kyungho Woo ◽  
Surya Surendran ◽  
Chul Choi
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Efflux Pump ◽  
Financial Burden ◽  
Multidrug Efflux ◽  
Multidrug Efflux Pump ◽  
Persistent Infections ◽  
Healthcare Settings ◽  
Recent Advances ◽  
Multidrug Efflux Pumps

Biofilm formation in healthcare is an issue of considerable concern, as it results in increased morbidity and mortality, imposing a significant financial burden on the healthcare system. Biofilms are highly resistant to conventional antimicrobial therapies and lead to persistent infections. Hence, there is a high demand for novel strategies other than conventional antibiotic therapies to control biofilm-based infections. There are two approaches which have been employed so far to control biofilm formation in healthcare settings: one is the development of biofilm inhibitors based on the understanding of the molecular mechanism of biofilm formation, and the other is to modify the biomaterials which are used in medical devices to prevent biofilm formation. This review will focus on the recent advances in anti-biofilm approaches by interrupting the quorum-sensing cellular communication system and the multidrug efflux pumps which play an important role in biofilm formation. Research efforts directed towards these promising strategies could eventually lead to the development of better anti-biofilm therapies than the conventional treatments.

scholarly journals Bicyclic brominated furanones: A new class of quorum sensing modulators that inhibit bacterial biofilm formation

2014 ◽  
Vol 22 (4) ◽  
pp. 1313-1317 ◽  
Author(s):  
Sijie Yang ◽  
Osama A. Abdel-Razek ◽  
Fei Cheng ◽  
Debjyoti Bandyopadhyay ◽  
Gauri S. Shetye ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
New Class ◽  
Brominated Furanones

scholarly journals Mutation of luxS Affects Biofilm Formation in Streptococcus mutans

2003 ◽  
Vol 71 (4) ◽  
pp. 1972-1979 ◽  
Author(s):  
Justin Merritt ◽  
Fengxia Qi ◽  
Steven D. Goodman ◽  
Maxwell H. Anderson ◽  
Wenyuan Shi
Keyword(s):  
Quorum Sensing ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Microscopic Analysis ◽  
Homoserine Lactone ◽  
Good Evidence ◽  
Genome Project ◽  
Bacterial Biofilm ◽  
Wild Type

ABSTRACT Quorum sensing is a bacterial mechanism for regulating gene expression in response to changes in population density. Many bacteria are capable of acyl-homoserine lactone-based or peptide-based intraspecies quorum sensing and luxS-dependent interspecies quorum sensing. While there is good evidence about the involvement of intraspecies quorum sensing in bacterial biofilm, little is known about the role of luxS in biofilm formation. In this study, we report for the first time that luxS-dependent quorum sensing is involved in biofilm formation of Streptococcus mutans. S. mutans is a major cariogenic bacterium in the multispecies bacterial biofilm commonly known as dental plaque. An ortholog of luxS for S. mutans was identified using the data available in the S. mutans genome project (http://www.genome.ou.edu/smutans.html ). Using an assay developed for the detection of the LuxS-associated quorum sensing signal autoinducer 2 (AI-2), it was demonstrated that this ortholog was able to complement the luxS negative phenotype of Escherichia coli DH5α. It was also shown that AI-2 is indeed produced by S. mutans. AI-2 production is maximal during mid- to late-log growth in batch culture. Mutant strains devoid of the luxS gene were constructed and found to be defective in producing the AI-2 signal. There are also marked phenotypic differences between the wild type and the luxS mutants. Microscopic analysis of in vitro-grown biofilm structure revealed that the luxS mutant biofilms adopted a much more granular appearance, rather than the relatively smooth, confluent layer normally seen in the wild type. These results suggest that LuxS-dependent signal may play an important role in biofilm formation of S. mutans.

scholarly journals Anti-Quorum Sensing Activity of 12 Essential Oils on chromobacterium violaceum and Specific Action of cis-cis-p-Menthenolide from Corsican Mentha suaveolens ssp. Insularis

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2125 ◽  
Author(s):  
Jean-Pierre Poli ◽  
Elodie Guinoiseau ◽  
Dominique de Rocca Serra ◽  
Sylvain Sutour ◽  
Mathieu Paoli ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Essential Oils ◽  
Biofilm Formation ◽  
Inhibitory Concentration ◽  
Chromobacterium Violaceum ◽  
Sea Islands ◽  
Bioactive Substances ◽  
Bacterial Communication ◽  
Enhanced Resistance ◽  
Violacein Production

Quorum sensing (QS) is a bacterial communication mechanism used to express various survival or virulence traits leading to enhanced resistance. Chromobacterium violaceum is a commonly used strain that highlights anti-QS action of bioactive substances. Here, we wanted to see if 12 selected essential oils (EO) could exert anti-QS activity. We measured the sublethal minimal QS inhibitory concentration (MQSIC) by assessing violacein production of C. violaceum along with bacterial growth. To confirm the QS disruption, we also proceed to surface bacterial observations using scanning electron microscopy (SEM). We showed that cis-cis-p-menthenolide extracted and isolated from a plant endemic to occidental Mediterranean Sea islands, Mentha suaveolens ssp. insularis, acts as an inhibitor of violacein production and biofilm formation. Measured MQSIC was much lower than the minimal inhibitory concentration (MIC): 0.10 mg·mL−1 vs. 3.00 mg·mL−1. Moreover, disturbance of QS-related traits was confirmed by the degradation of C. violaceum biofilm matrix. There is a clear structure–activity relationship between cis-cis-p-menthenolide and anti-QS activity. Indeed, its isomer molecule (mintlactone) exerts a poor anti-QS action. These results indicate that inhibition of violacein production and biofilm formation by cis-cis-p-menthenolide might be related to a disruption in the QS mechanism.

scholarly journals Use of Quorum Sensing Inhibition Strategies to Control Microfouling

Marine Drugs ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 74
Author(s):  
Andrea Muras ◽  
Ana Parga ◽  
Celia Mayer ◽  
Ana Otero
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Communication Systems ◽  
Culture Medium ◽  
Homoserine Lactone ◽  
Bacterial Biofilm ◽  
Screening Methods ◽  
Time Saving ◽  
Quorum Sensing Inhibition

Interfering with the quorum sensing bacterial communication systems has been proposed as a promising strategy to control bacterial biofilm formation, a key process in biofouling development. Appropriate in vitro biofilm-forming bacteria models are needed to establish screening methods for innovative anti-biofilm and anti-microfouling compounds. Four marine strains, two Pseudoalteromonas spp. and two Vibrio spp., were selected and studied with regard to their biofilm-forming capacity and sensitivity to quorum sensing (QS) inhibitors. Biofilm experiments were performed using two biofilm cultivation and quantification methods: the xCELLigence® system, which allows online monitoring of biofilm formation, and the active attachment model, which allows refreshment of the culture medium to obtain a strong biofilm that can be quantified with standard staining methods. Although all selected strains produced acyl-homoserine-lactone (AHL) QS signals, only the P. flavipulchra biofilm, measured with both quantification systems, was significantly reduced with the addition of the AHL-lactonase Aii20J without a significant effect on planktonic growth. Two-species biofilms containing P. flavipulchra were also affected by the addition of Aii20J, indicating an influence on the target bacterial strain as well as an indirect effect on the co-cultured bacterium. The use of xCELLigence® is proposed as a time-saving method to quantify biofilm formation and search for eco-friendly anti-microfouling compounds based on quorum sensing inhibition (QSI) strategies. The results obtained from these two in vitro biofilm formation methods revealed important differences in the response of biosensor bacteria to culture medium and conditions, indicating that several strains should be used simultaneously for screening purposes and the cultivation conditions should be carefully optimized for each specific purpose.

scholarly journals Identification of Small Molecules That Antagonize Diguanylate Cyclase Enzymes To Inhibit Biofilm Formation

2012 ◽  
Vol 56 (10) ◽  
pp. 5202-5211 ◽  
Author(s):  
Karthik Sambanthamoorthy ◽  
Rudolph E. Sloup ◽  
Vijay Parashar ◽  
Joshua M. Smith ◽  
Eric E. Kim ◽  
...  
Keyword(s):  
Small Molecules ◽  
Biofilm Formation ◽  
Flow System ◽  
Total Concentration ◽  
Bacterial Biofilm ◽  
Diguanylate Cyclase ◽  
Signaling System ◽  
Chronic Infections ◽  
Content Type ◽  
New Strategies

ABSTRACTBacterial biofilm formation is responsible for numerous chronic infections, causing a severe health burden. Many of these infections cannot be resolved, as bacteria in biofilms are resistant to the host's immune defenses and antibiotic therapy. New strategies to treat biofilm-based infections are critically needed. Cyclic di-GMP (c-di-GMP) is a widely conserved second-messenger signal essential for biofilm formation. As this signaling system is found only in bacteria, it is an attractive target for the development of new antibiofilm interventions. Here, we describe the results of a high-throughput screen to identify small-molecule inhibitors of diguanylate cyclase (DGC) enzymes that synthesize c-di-GMP. We report seven small molecules that antagonize these enzymes and inhibit biofilm formation byVibrio cholerae. Moreover, two of these compounds significantly reduce the total concentration of c-di-GMP inV. cholerae, one of which also inhibits biofilm formation byPseudomonas aeruginosain a continuous-flow system. These molecules represent the first compounds described that are able to inhibit DGC activity to prevent biofilm formation.

scholarly journals Design, Synthesis, and Evaluation of Alkyl-Quinoxalin-2(1H)-One Derivatives as Anti-Quorum Sensing Molecules, Inhibiting Biofilm Formation in Aeromonas caviae Sch3

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3075 ◽  
Author(s):  
René Blöcher ◽  
Ariel Rodarte Ramírez ◽  
Graciela Castro-Escarpulli ◽  
Everardo Curiel-Quesada ◽  
Alicia Reyes-Arellano
Keyword(s):  
Carboxylic Acid ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Alternative Methods ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Design Synthesis ◽  
Bacterial Communication ◽  
Aeromonas Caviae

With the increasing antibiotic resistance of bacterial strains, alternative methods for infection control are in high demand. Quorum sensing (QS) is the bacterial communication system based on small molecules. QS is enables bacterial biofilm formation and pathogenic development. The interruption of QS has become a target for drug discovery, but remains in the early experimental phase. In this study, we synthesized a set of six compounds based on a scaffold (alkyl-quinoxalin-2(1H)-one), new in the anti-QS of Gram-negative bacteria Aeromonas caviae Sch3. By quantifying biofilm formation, we were able to monitor the effect of these compounds from concentrations of 1 to 100 µM. Significant reduction in biofilm formation was achieved by 3-hexylylquinoxalin-2(1H)-one (11), 3-hexylylquinoxalin-2(1H)-one-6-carboxylic acid (12), and 3-heptylylquinoxalin-2(1H)-one-6-carboxylic acid (14), ranging from 11% to 59% inhibition of the biofilm. This pilot study contributes to the development of anti-QS compounds to overcome the clinical challenge of resistant bacteria strains.

scholarly journals Acetic Acid Acts as a Volatile Signal To Stimulate Bacterial Biofilm Formation

mBio ◽  
2015 ◽  
Vol 6 (3) ◽  
Author(s):  
Yun Chen ◽  
Kevin Gozzi ◽  
Fang Yan ◽  
Yunrong Chai
Keyword(s):  
Acetic Acid ◽  
Bacillus Subtilis ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Molecular Mechanisms ◽  
Biological Activities ◽  
Bacterial Biofilm ◽  
Acetic Acid Production ◽  
Content Type ◽  
Metabolic Signal

ABSTRACTVolatiles are small air-transmittable chemicals with diverse biological activities. In this study, we showed that volatiles produced by the bacteriumBacillus subtilishad a profound effect on biofilm formation of neighboringB. subtiliscells that grew in proximity but were physically separated. We further demonstrated that one such volatile, acetic acid, is particularly potent in stimulating biofilm formation. Multiple lines of genetic evidence based onB. subtilismutants that are defective in either acetic acid production or transportation suggest thatB. subtilisuses acetic acid as a metabolic signal to coordinate the timing of biofilm formation. Lastly, we investigated howB. subtiliscells sense and respond to acetic acid in regulating biofilm formation. We showed the possible involvement of three sets of genes (ywbHG,ysbAB, andyxaKC), all encoding putative holin-antiholin-like proteins, in cells responding to acetic acid and stimulating biofilm formation. All three sets of genes were induced by acetate. A mutant with a triple mutation of those genes showed a severe delay in biofilm formation, whereas a strain overexpressingywbHGshowed early and robust biofilm formation. Results of our studies suggest thatB. subtilisand possibly other bacteria use acetic acid as a metabolic signal to regulate biofilm formation as well as a quorum-sensing-like airborne signal to coordinate the timing of biofilm formation by physically separated cells in the community.IMPORTANCEVolatiles are small, air-transmittable molecules produced by all kingdoms of organisms including bacteria. Volatiles possess diverse biological activities and play important roles in bacteria-bacteria and bacteria-host interactions. Although volatiles can be used as a novel and important way of cell-cell communication due to their air-transmittable nature, little is known about how the volatile-mediated signaling mechanism works. In this study, we demonstrate that the bacteriumBacillus subtilisuses one such volatile, acetic acid, as a quorum-sensing-like signal to coordinate the timing of the formation of structurally complex cell communities, also known as biofilms. We further characterized the molecular mechanisms of howB. subtilisresponds to acetic acid in stimulating biofilm formation. Our study also suggests that acetic acid may be used as a volatile signal for cross-species communication.

scholarly journals Quorum sensing inhibitory effects of vanillin on the biofilm formation of Pseudomonas fluorescens P07 by transcriptome analysis

2021 ◽  
Vol 5 (7) ◽  
pp. 275-292
Author(s):  
Ting Ding ◽  
◽  
Yong Li
Keyword(s):  
Quorum Sensing ◽  
Pseudomonas Fluorescens ◽  
Transcriptome Analysis ◽  
Biofilm Formation ◽  
Homoserine Lactone ◽  
Bacterial Biofilm ◽  
Inhibitory Effects ◽  
Swarming Motility ◽  
Physiological Processes ◽  
Spoilage Bacterium

Pseudomonas fluorescens is an important psychrotrophic food-spoilage bacterium. Quorum sensing (QS) enables bacteria to control various physiological processes. Hence, targeting bacterial QS would be a novel method to improve food quality. In this study, P. fluorescens P07 was treated with vanillin, which showed strong QS inhibitory activity, and its resultant effects on swarming motility, biofilm formation, and extracellular polymeric substance (EPS) secretion were measured. The mechanisms underlying the inhibitory effects were then explored by transcriptomic analysis. The results showed that vanillin had inhibitory effects on swarming motility, biofilm formation, N-acyl-L-homoserine Lactone (AHLs) and EPS secretion of P. fluorescens P07. The result of transcriptionomic tests indicated that the decrease in bacterial biofilm formation was probably due to the influence of vanillin on mobility, adhesion, chemotaxis, EPS secretion, and QS system of the bacteria. Keywords: Pseudomonas fluorescens, quorum sensing, biofilm formation, transcriptome analysis, swarming motility

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