scholarly journals Nontargeted Metabolomics for Phenolic and Polyhydroxy Compounds Profile of Pepper (Piper nigrum L.) Products Based on LC-MS/MS Analysis

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1985 ◽  
Author(s):  
Fenglin Gu ◽  
Guiping Wu ◽  
Yiming Fang ◽  
Hongying Zhu
Keyword(s):  
Principal Component Analysis ◽  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Principal Component ◽  
Quinic Acid ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Polyhydroxy Compounds ◽  
Freeze Dried ◽  
Metabolite Profiles

In the present study, nontargeted metabolomics was used to screen the phenolic and polyhydroxy compounds in pepper products. A total of 186 phenolic and polyhydroxy compounds, including anthocyanins, proanthocyanidins, catechin derivatives, flavanones, flavones, flavonols, isoflavones and 3-O-p-coumaroyl quinic acid O-hexoside, quinic acid (polyhydroxy compounds), etc. For the selected 50 types of phenolic compound, except malvidin 3,5-diglucoside (malvin), l-epicatechin and 4′-hydroxy-5,7-dimethoxyflavanone, other compound contents were present in high contents in freeze-dried pepper berries, and pinocembrin was relatively abundant in two kinds of pepper products. The score plots of principal component analysis indicated that the pepper samples can be classified into four groups on the basis of the type pepper processing. This study provided a comprehensive profile of the phenolic and polyhydroxy compounds of different pepper products and partly clarified the factors responsible for different metabolite profiles in ongoing studies and the changes of phenolic compounds for the browning mechanism of black pepper.

scholarly journals Morphological characterization and secondary metabolites profile of black pepper (Piper nigrum L.) genotypes from Sikkim

2020 ◽  
pp. 98-104
Author(s):  
K. Gurung ◽  
S. Manivannan
Keyword(s):  
Principal Component Analysis ◽  
Secondary Metabolites ◽  
Crop Improvement ◽  
Principal Component ◽  
Volatile Oil ◽  
Black Pepper ◽  
Morphological Characterization ◽  
Piper Nigrum ◽  
Sikkim Himalaya ◽  
Improvement Strategies

Quantification of volatile oil and analysis of four major metabolites using HPLC was done in 24 black pepper genotypes collected from south Sikkim. The amount of volatile oil ranged from 2.01% to 0.022%. Secondary metabolites like piperine ranged from 2.75-0.022%, myrcene from 2.094-0.022%, alpha- phellandrene from 1.373-0.008% and linalool from 0.834-0.012%. Genotype 23 had the highest amount of myrcene and linalool, genotype 13 had the highest quantity of piperine and genotype 8 had high amount of alpha-phellandrene. The principal component analysis (PCA) of analyzed metabolites grouped the genotypes into four categories. The study revealed that some of the genotypes were as good as pepper varieties grown in traditional areas. These genotypes will be useful in crop improvement strategies and suitable for Sikkim Himalaya.

scholarly journals Variability in the Content of Phenolic Compounds in Plum Fruit

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1611
Author(s):  
Mindaugas Liaudanskas ◽  
Rugilė Okulevičiūtė ◽  
Juozas Lanauskas ◽  
Darius Kviklys ◽  
Kristina Zymonė ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Cluster Analysis ◽  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Quinic Acid ◽  
Fruit Trees ◽  
Quantitative Content ◽  
Fruit Samples

The aim of this study was to determine the composition and content of phenolic compounds in extracts of plum fruit. Fruit of 17 plum cultivars were analyzed. Fruit samples were collected in 2019 from fruit trees with “Myrobalan” (P. cerasifera Ehrh.) and “Wangenheim Prune” (P. domestica L.) rootstocks. The following glycosides of the flavonol group were identified: avicularin, isorhamnetin-3-O-rutinoside, isoquercitrin, hyperoside, rutin, and an aglycone quercetin. Compounds of the flavan-3-ol group were identified, such as (+)-catechin, procyanidin C1, and procyanidin A2, along with chlorogenic acid attributed to phenolic acids and a non-phenolic cyclitol–quinic acid. Of all the analytes identified in plum fruit samples, quinic acid predominated, while chlorogenic acid predominated among all the identified phenolic compounds, and rutin predominated in the flavonol group. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) revealed that fruit samples of “Kubanskaya Kometa”, “Zarechnaya Raniaya”, “Duke of Edinburgh”, “Jubileum”, and “Favorita del Sultano” cultivars had different quantitative content of phenolic compounds from that observed in other samples. The highest total amount of phenolic compounds was found in the European plum samples of the “Zarechnaya Rannyaya” cultivar, while the amount of quinic acid was the highest in plum fruit samples of the “Jubileum” cultivar.

scholarly journals Relationships Between the Content of Phenolic Compounds and the Antioxidant Activity of Polish Honey Varieties as a Tool for Botanical Discrimination

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1810
Author(s):  
Monika Kędzierska-Matysek ◽  
Małgorzata Stryjecka ◽  
Anna Teter ◽  
Piotr Skałecki ◽  
Piotr Domaradzki ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Caffeic Acid ◽  
Principal Component ◽  
Total Content ◽  
Component Analysis ◽  
Total Phenolic ◽  
Hydroxybenzoic Acid ◽  
Coumaric Acid

The study compared the content of eight phenolic acids and four flavonoids and the antioxidant activity of six Polish varietal honeys. An attempt was also made to determine the correlations between the antioxidant parameters of the honeys and their polyphenol profile using principal component analysis. Total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (ABTS) and reduction capacity (FRAP) were determined spectrophotometrically, and the phenolic compounds were determined using high-performance liquid chromatography (HPLC). The buckwheat honeys showed the strongest antioxidant activity, most likely because they had the highest concentrations of total phenols, total flavonoids, p-hydroxybenzoic acid, caffeic acid, p-coumaric acid, vanillic acid and chrysin. The principal component analysis (PCA) of the data showed significant relationships between the botanic origin of the honey, the total content of phenolic compounds and flavonoids and the antioxidant activity of the six Polish varietal honeys. The strongest, significant correlations were shown for parameters of antioxidant activity and TPC, TFC, p-hydroxybenzoic acid, caffeic acid and p-coumaric acid. Analysis of four principal components (explaining 86.9% of the total variance), as a classification tool, confirmed the distinctiveness of the Polish honeys in terms of their antioxidant activity and content of phenolic compounds.

Piper nigrum Oil – Determination of Selected Terpenes for Quality Evaluation

Planta Medica ◽  
2018 ◽  
Vol 85 (03) ◽  
pp. 185-194 ◽  
Author(s):  
Mei Wang ◽  
Amar Chittiboyina ◽  
Jon Parcher ◽  
Zulfiqar Ali ◽  
Paul Ford ◽  
...  
Keyword(s):  
Quality Evaluation ◽  
Geographical Location ◽  
Chemical Characterization ◽  
Principal Component ◽  
Cost Effective ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Ms Analysis ◽  
Chemical Profiles ◽  
High Concentrations

AbstractThe growing demand and commercial value of black pepper (Piper nigrum) has resulted in considerable interest in developing suitable and cost-effective methods for chemical characterization and quality evaluation purposes. In the current study, an extensive set of oil samples (n = 23) that were extracted by steam distillation from black pepper seeds was investigated to compare the chemical profiles of samples originating from nine major producing countries, as well as to identify potential chemical markers for quality evaluation. The twenty-two most abundant volatile compounds, mainly terpenes, in these oils were determined by conventional GC/MS analysis. Principal component analysis with this set of data revealed distinct clusters for samples that originated from China and Malaysia. Relatively low concentrations of sabinene (< 0.2%) and high concentrations of 3-carene (10.9 – 21.1%) were observed in these samples, respectively, compared to oil samples from other countries. The enantiomeric distributions of key terpene markers, viz., β-pinene, sabinene, limonene, and terpinen-4-ol, were determined by chiral GC/MS analysis. Interestingly, for these four monoterpenes, levo-isomers were found to be predominant, emphasizing the highly conserved enzymatic processes occurring in P. nigrum. Moreover, consistent enantiomeric ratios ((−) isomer/(+) isomer) of 92.2 ± 3.0% for β-pinene, 94.8 ± 2.8% for sabinene, 60.7 ± 1.1% for limonene, and 78.3 ± 1.3% for terpinen-4-ol were observed, independent of geographical location. These results demonstrate the potential of using stereospecific compositions as chiral signatures for establishing the authenticity and quality of black pepper oil.

scholarly journals Antioxidants and Phenolic Secretion in Sugarcane Genotypes Shoots Culture

2018 ◽  
Vol 10 (5) ◽  
pp. 79
Author(s):  
Ana Da Silva Ledo ◽  
Maria M. Jenderek ◽  
Carlos Alberto Da Silva Ledo ◽  
Tomas Ayala-Silva
Keyword(s):  
Principal Component Analysis ◽  
Citric Acid ◽  
Phenolic Compounds ◽  
Shoot Culture ◽  
Shoot Proliferation ◽  
Principal Component ◽  
Shoot Formation ◽  
Regenerative Capacity ◽  
In Vitro Shoot Culture

Secretion of phenolic compounds is a major limitation for sugarcane in vitro shoot culture, causing a loss of regenerative capacity and subsequent cell death. In this study, micropropagation and phenolic secretion of four Saccharum genotypes were evaluated in presence of different antioxidants. Aseptic cultures of S. officinarum (PI 184794 and PI 88652), S. sinense (PI 29109) and S. robustum (UNK R65P35) were propagated on medium containing antioxidants, citric acid (100 mg/L), L-cysteine (100 mg/L), polyvynylpirrolidone (300 mg/L) and L-glutathione (50 mg/L) in two consecutive subculture cycles. Interaction between genotypes and antioxidants was significant in both cycles. All genotypes showed good shoot formation, shoot vigor and color, except in PI 88652 which had less shoot development in both the presence and absence of the antioxidants tested. PI 184794 displayed the highest shoot proliferation in the presence of citric acid, and UNK R65P35 produced more shoots per explant in the 2nd subculture. For S. sinense (PI 29109), in both subcultures, most shoots were observed in the presence of polyvynylpirrolidone. Medium discoloration due to phenolic secretion was reduced in the presence of citric acid and polyvynylpirrolidone. The type of secreted phenolic compounds differed with genotype as the Principal Component Analysis of cultivation media separated PI 88652 from PI 29109 and UKN R65P35. Phenolic compounds varied in composition and were secreted at various levels as a function of genotype and antioxidant type. Loadings plots indicated the genotype and antioxidant separations were broadly driven by flavonoid compounds.

scholarly journals Morphological Traits and Phenolic Compounds in Tunisian Wild Populations and Cultivated Varieties of Portulaca oleracea L.

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 948 ◽  
Author(s):  
Dorra Sdouga ◽  
Ferdinando Branca ◽  
Souhir Kabtni ◽  
Maria Concetta Di Bella ◽  
Neila Trifi-Farah ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Geographic Origin ◽  
Sinapic Acid ◽  
Principal Component ◽  
Portulaca Oleracea ◽  
Array Detector ◽  
Wild Populations ◽  
Antioxidant Power ◽  
Morphological Heterogeneity ◽  
Freeze Dried

This study aims to evaluate the bio-morphological and biochemical variability of three Tunisian wild populations and one growing cultivar of purslane (Portulaca oleracea L.). The studied varieties should be easily distinguished by the color and the habitus of the plant as mentioned in literature, but the various analyses have shown a strong morphological heterogeneity within and among the wild and cultivated accessions as presented by the variance analysis test (ANOVA) and the PCA (Principal component analysis). We found high intrapopulation variability through the wild populations that make it hard to differentiate them only on the base of morphology. We analyzed the biochemical profile of those populations based on the analysis of freeze-dried samples of leaves and stems. We identified and quantified twelve different phenolic compounds by the HPLC-diode array detector (DAD) technique. Six phenolic acids and flavonoids were identified in the leaves and stems of the wild and cultivated populations. Sinapic acid and myricetin are the majors identified compounds through our samples. The results were significantly different in relation to the plant organs and to the geographic origin for most of the compounds. The obtained results highlighted the importance of Portulaca as a medicinal plant by showing its richness in phenols and flavonoids that have multi-medicinal effects besides their antioxidant power.

scholarly journals Phytochemical and Antioxidant Studies on a Rare Rheum cordatum Losinsk. Species from Kazakhstan

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Gulsim Zhumashova ◽  
Wirginia Kukula-Koch ◽  
Wojciech Koch ◽  
Tomasz Baj ◽  
Galiya Sayakova ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Epigallocatechin Gallate ◽  
Principal Component ◽  
Epicatechin Gallate ◽  
Chemical Content ◽  
Time Of Collection ◽  
Tof Ms

An optimisation of extraction towards an increased antioxidant capacity and the study on the extracts’ composition by HPLC-ESI-Q-TOF-MS were performed on different organs of a rarely studied plant: Rheum cordatum Losinsk (Polygonaceae) growing in Kazakhstan. More than 20 compounds from anthraquinones and phenolics were identified in an optimised method. The plant was proven to contain a wide variety of phenolic compounds (catechins, flavonoids, and their glucosides and phenolic acids) in contrast to the anthraquinone composition, which was mainly represented by emodin and its analogues. The results of the studies could determine the plant as a rich source of pharmacologically precious polyphenols. It was evidenced that the extracting solvents, the time of collection, and the organs tested affected both the chemical content and the antioxidant potential of the extracts. Ethanol : water (50 : 50 v/v) was selected as the most beneficial extractant for all metabolites, and based on the principal component analysis of raw data, the radical scavenging potential of the plant was strictly related to the presence of epicatechin gallate (ECG), kaempferol glucoside, and epigallocatechin gallate (EGCG) occurring in this extract at the concentration of 1.69-5%, 0.16-0.47%, and 0.001-2.93%, respectively.

scholarly journals Comparison of Phenolic Compounds and the Antioxidant Activities of Fifteen Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’ in China

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 325 ◽  
Author(s):  
Jinyan Gong ◽  
Bingquan Chu ◽  
Lingxiao Gong ◽  
Zhongxiang Fang ◽  
Xiaoxu Zhang ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
High Performance Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Principal Component ◽  
Chrysanthemum Morifolium ◽  
Caffeoylquinic Acids ◽  
Caffeoylquinic Acid ◽  
Chrysanthemum Morifolium Ramat

This study investigated the phenolic compounds of 15 Chrysanthemum morifolium Ramat cv. ‘Hangbaiju’, including 6 ‘Duoju’ and 9 ‘Taiju’, using high performance liquid chromatography (HPLC). The antioxidant activities of these ‘Hangbaiju’ were estimated by DPPH, ABTS and FRAP assays. Results show that a total of 14 phenolic compounds were detected in these flowers, including 3 mono-caffeoylquinic acids, 3 di-caffeoylquinic acids, 1 phenolic acid and 7 flavonoids. ‘Duoju’ and ‘Taiju’ possess different concentrations of phenolic compounds, and ‘Taiju’ exhibits higher caffeoylquinic acids and stronger antioxidant activities than ‘Duoju’. Caffeoylquinic acids show a strong correlation with the antioxidant activities of the samples. Principal component analysis (PCA) reveals an obvious separation between ‘Duoju’ and ‘Taiju’, using phenolic compounds as variables. Apigenin-7-O-glucoside, 3,5-di-O-caffeoylquinic acid, luteolin and acacetin were found to be the key phenolic compounds to differentiate ‘Duoju’ from ‘Taiju’.

Impacts des milieux pédologiques en bosses et creux sur les teneurs en composés phénoliques et en éléments minéraux dans les feuilles d'érables à sucre (Acersaccharum) en dépérissement du Québec

1993 ◽  
Vol 23 (2) ◽  
pp. 190-198 ◽  
Author(s):  
A. Sauvesty ◽  
F. Pagé ◽  
M. Giroux
Keyword(s):  
Principal Component Analysis ◽  
Phenolic Compounds ◽  
Chemical Analysis ◽  
Sugar Maple ◽  
Principal Component ◽  
Component Analysis ◽  
Mineral Elements ◽  
Total N ◽  
Site Distribution ◽  
Foliar N

We conducted a study on the influence of microrelief on the content of phenolic compounds and mineral elements in leaves of sugar maple (Acersaccharum Marsh.) at various stages of decline. The stand of origin was located in the Appalachian Highlands of Quebec, on acidic material with a microrelief constituted of hollows and bumps, the height of the latter varying between 30 and 60 cm. Well aerated podzols developed on bumps, while gleysols developed in hollows. Trees generally grew on bumps and their roots could extend as far as 5 m away from the tree. Microrelief and soils were described within a 5-m radius around every sugar maple under study. Pedological sites were classified into three categories according to their microrelief (percent surface area covered by bumps): (i) less than 30% of surface (dominance of poorly drained soils), (ii) between 30 and 50% of surface (dominance of imperfectly drained soils), and (iii) more than 50% of surface (dominance of well drained soils). A principal component analysis on site distribution and a chemical analysis of soils showed an increase in total N and in K, Ca, and Mg availability with an increase in soil humidity. The principal component analysis and chemical analysis of leaves indicated that the content of phenolic compounds was highest, and those of N and K lowest, in moistest soils. The decline was also greatest on those sites. There was a strong correlation between synthesis of phenolic compounds and foliar N contents, and between decline intensity and foliar N content. The high humidity of some soils of the study sites reduces the availability of N, causing a stress to sugar maples. This phenomenon would result in an increased synthesis of phenolic compounds and a decline in less resistant trees. Phenolic compounds could then be utilized as a physiological indicator of stress. Those results explain why healthy trees can grow next to declining trees.

Sign in / Sign up

Export Citation Format

Share Document