scholarly journals Phytochemical and Antioxidant Studies on a Rare Rheum cordatum Losinsk. Species from Kazakhstan

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Gulsim Zhumashova ◽  
Wirginia Kukula-Koch ◽  
Wojciech Koch ◽  
Tomasz Baj ◽  
Galiya Sayakova ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Epigallocatechin Gallate ◽  
Principal Component ◽  
Epicatechin Gallate ◽  
Chemical Content ◽  
Time Of Collection ◽  
Tof Ms

An optimisation of extraction towards an increased antioxidant capacity and the study on the extracts’ composition by HPLC-ESI-Q-TOF-MS were performed on different organs of a rarely studied plant: Rheum cordatum Losinsk (Polygonaceae) growing in Kazakhstan. More than 20 compounds from anthraquinones and phenolics were identified in an optimised method. The plant was proven to contain a wide variety of phenolic compounds (catechins, flavonoids, and their glucosides and phenolic acids) in contrast to the anthraquinone composition, which was mainly represented by emodin and its analogues. The results of the studies could determine the plant as a rich source of pharmacologically precious polyphenols. It was evidenced that the extracting solvents, the time of collection, and the organs tested affected both the chemical content and the antioxidant potential of the extracts. Ethanol : water (50 : 50 v/v) was selected as the most beneficial extractant for all metabolites, and based on the principal component analysis of raw data, the radical scavenging potential of the plant was strictly related to the presence of epicatechin gallate (ECG), kaempferol glucoside, and epigallocatechin gallate (EGCG) occurring in this extract at the concentration of 1.69-5%, 0.16-0.47%, and 0.001-2.93%, respectively.

Antioxidant Evaluation and Composition Analysis of Extracts from Fuzhuan Brick Tea and Its Comparison with Two Instant Tea Products

2017 ◽  
Vol 100 (3) ◽  
pp. 653-660 ◽  
Author(s):  
Qing-An Zhang ◽  
Xiao-Li Zhang ◽  
Yan-Ying Yan ◽  
Xue-Hui Fan
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Hplc Analysis ◽  
Epigallocatechin Gallate ◽  
Principal Component ◽  
Composition Analysis ◽  
Antioxidant Evaluation ◽  
High Level

Abstract In this paper, the antioxidant capacities and compositions of two commercialized tea products and extracts from Fuzhuan brick tea (FBT) were investigated using three HPLC methods comparing the retention times of injected standards. Principal component analysis and DPPH-spiking HPLC analysis were used to analyze correlation between antioxidant capacity and the compounds detected to screen which compounds contribute to antioxidant activity. Results indicated that all samples contained high amounts of polysaccharides, phenols, and flavonoids and had good antioxidant activity and a high level of correlation among them. Furthermore, gallic acid, epigallocatechin, epicatechin, and epigallocatechin gallate were screened and found to be stronger antioxidant candidates. In summary, the quality of the FBT extracts was not inferior to that of commercialized tea products, suggesting the feasibility that extracts may directly act as instant tea products.

scholarly journals The Galloyl Catechins Contributing to Main Antioxidant Capacity of Tea Made fromCamellia sinensisin China

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Chunjian Zhao ◽  
Chunying Li ◽  
Shuaihua Liu ◽  
Lei Yang
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Epigallocatechin Gallate ◽  
Dpph Radical ◽  
Polyphenol Content ◽  
Epicatechin Gallate ◽  
Total Polyphenol ◽  
Total Polyphenol Content ◽  
Antioxidant Capacities ◽  
Dpph Radical Scavenging

Total polyphenol content, catechins content, and antioxidant capacities of green, dark, oolong, and black teas made fromCamellia sinensisin China were evaluated. The total polyphenol content of 20 samples of tea was in the range of 7.82–32.36%. Total catechins content was in the range of 4.34–24.27%. The antioxidant capacity of tea extract was determined by the oxygen radical absorbance capacity (ORAC) test and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging test. Total polyphenol content, catechins content, and antioxidant capacity decreased in the following order: green > oolong > black > dark tea. A positive correlation existed between the antioxidant capacity and total polyphenol content or catechins content (R2=0.67–0.87). The antioxidant capacities of five major catechins (epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epicatechin, epigallocatechin, and catechin) were determined by online HPLC DPPH radical-scavenging; the antioxidant activity of tea was mainly attributed to the esterified catechins (EGCG or ECG).

scholarly journals Bioactive Compound Fingerprint Analysis of Aged Raw Pu’er Tea and Young Ripened Pu’er Tea

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1931 ◽  
Author(s):  
Vasilisa Pedan ◽  
Sascha Rohn ◽  
Mirjam Holinger ◽  
Tilo Hühn ◽  
Irene Chetschik
Keyword(s):  
Principal Component Analysis ◽  
Gallic Acid ◽  
Bioactive Compounds ◽  
Epigallocatechin Gallate ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Bioactive Compound ◽  
Component Analysis ◽  
Epicatechin Gallate ◽  
Total Polyphenols

Pu’er tea produced from Camellia sinensis var. assamica is a widely appreciated and consumed beverage that can be divided into two kinds of tea depending on the different fermentation processed used, the special sensory characteristics, and their chemical composition. However, authentication seems to be very important for such teas, as they are traded to comparatively high prices, especially in Europe. The results for selected biochemical markers showed that aged raw pu’er tea contained 210.2 mg GAE/g polyphenols, of which 2.2 mg/g were gallic acid, 16.1 mg/g theogallin, 35.1 mg/g (−)-epigallocatechin gallate, and 40.1 mg/g (−)-epicatechin gallate, on average. Young ripened pu’er tea contained about 104.6 mg GAE/g polyphenols, of which 5.5 mg/g gallic acid, 0.9 mg/g theogallin, 0.7 mg/g (−)-epigallocatechin gallate, and 1.8 mg/g (−)-epicatechin gallate, on average. An additional objective of the present study was to unravel the best brewing conditions for optimal extraction of the bioactive compounds. Infusions of nineteen commercial teas (from pu’er cakes) were obtained at different time-temperature ratios for studying the content of bioactive compounds (flavan-3-ols, flavonols, caffeoylquinic acids, methylxanthines). Brewing at 90 °C for 5 min was the best condition to obtain a high content of total polyphenols in ripened pu’er tea. Principal component analysis and hierarchical cluster analysis showed, that young ripened and aged raw pu’er tea can be successfully differentiated by the analyzed chemical compounds. Principal component analysis results indicated that young ripened pu’er tea has higher contents of gallic acid, quercetin, and kaempferol than aged raw pu’er tea.

scholarly journals Relationships Between the Content of Phenolic Compounds and the Antioxidant Activity of Polish Honey Varieties as a Tool for Botanical Discrimination

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1810
Author(s):  
Monika Kędzierska-Matysek ◽  
Małgorzata Stryjecka ◽  
Anna Teter ◽  
Piotr Skałecki ◽  
Piotr Domaradzki ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Caffeic Acid ◽  
Principal Component ◽  
Total Content ◽  
Component Analysis ◽  
Total Phenolic ◽  
Hydroxybenzoic Acid ◽  
Coumaric Acid

The study compared the content of eight phenolic acids and four flavonoids and the antioxidant activity of six Polish varietal honeys. An attempt was also made to determine the correlations between the antioxidant parameters of the honeys and their polyphenol profile using principal component analysis. Total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (ABTS) and reduction capacity (FRAP) were determined spectrophotometrically, and the phenolic compounds were determined using high-performance liquid chromatography (HPLC). The buckwheat honeys showed the strongest antioxidant activity, most likely because they had the highest concentrations of total phenols, total flavonoids, p-hydroxybenzoic acid, caffeic acid, p-coumaric acid, vanillic acid and chrysin. The principal component analysis (PCA) of the data showed significant relationships between the botanic origin of the honey, the total content of phenolic compounds and flavonoids and the antioxidant activity of the six Polish varietal honeys. The strongest, significant correlations were shown for parameters of antioxidant activity and TPC, TFC, p-hydroxybenzoic acid, caffeic acid and p-coumaric acid. Analysis of four principal components (explaining 86.9% of the total variance), as a classification tool, confirmed the distinctiveness of the Polish honeys in terms of their antioxidant activity and content of phenolic compounds.

scholarly journals Color and Antioxidant Capacity Preservation of Opuntia spp. Juices by Spray-drying Microencapsulation.

2018 ◽  
Vol 7 (3) ◽  
pp. 16 ◽  
Author(s):  
Alberto Castañeda-Yañez ◽  
Sandra T. Martín-del-Campo ◽  
Alejandra San-Martin ◽  
Anaberta Cardador-Martínez
Keyword(s):  
Principal Component Analysis ◽  
Protective Effect ◽  
Antioxidant Capacity ◽  
Spray Drying ◽  
Principal Component ◽  
Natural Colorants ◽  
The Matrix ◽  
Pear Juice ◽  
Antioxidant Characteristics ◽  
Opuntia Spp

In this work, it was evaluated the effect of microencapsulation using spray drying over natural colorants present in two varieties (red and purple) of prickly pear juice (Opuntia spp.), using three kinds of carrier agents (matrixes). The dried samples after microencapsulation retained a high total amount of the betalains and their antioxidant characteristics. However, some individual betalains were lost after microencapsulation. According to ANOVA results, matrix 3204 showed a more protective effect than matrix 4801 in both microencapsulated juices over color, individual betalains, and antioxidant capacity. Globally, the protective effect was better for purple juices than red juices no matter the matrix used. Principal Component Analysis (PCA) confirmed these results. Matrix 3204 resulted in the best carrier agent since it gave a less disperse PCA group for both color juices. The parameters that separated both PCA matrixes groups were L*, a*, b* and DPPH. 

Scope and limitations of principal component analysis of high resolution LC-TOF-MS data: the analysis of the chlorogenic acid fraction in green coffee beans as a case study

2011 ◽  
Vol 3 (1) ◽  
pp. 144-155 ◽  
Author(s):  
Nikolai Kuhnert ◽  
Rakesh Jaiswal ◽  
Pinkie Eravuchira ◽  
Rasha M. El-Abassy ◽  
Bernd von der Kammer ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
High Resolution ◽  
Chlorogenic Acid ◽  
Principal Component ◽  
Green Coffee ◽  
Acid Fraction ◽  
Green Coffee Beans ◽  
Coffee Beans ◽  
Tof Ms

scholarly journals Antioxidants and Phenolic Secretion in Sugarcane Genotypes Shoots Culture

2018 ◽  
Vol 10 (5) ◽  
pp. 79
Author(s):  
Ana Da Silva Ledo ◽  
Maria M. Jenderek ◽  
Carlos Alberto Da Silva Ledo ◽  
Tomas Ayala-Silva
Keyword(s):  
Principal Component Analysis ◽  
Citric Acid ◽  
Phenolic Compounds ◽  
Shoot Culture ◽  
Shoot Proliferation ◽  
Principal Component ◽  
Shoot Formation ◽  
Regenerative Capacity ◽  
In Vitro Shoot Culture

Secretion of phenolic compounds is a major limitation for sugarcane in vitro shoot culture, causing a loss of regenerative capacity and subsequent cell death. In this study, micropropagation and phenolic secretion of four Saccharum genotypes were evaluated in presence of different antioxidants. Aseptic cultures of S. officinarum (PI 184794 and PI 88652), S. sinense (PI 29109) and S. robustum (UNK R65P35) were propagated on medium containing antioxidants, citric acid (100 mg/L), L-cysteine (100 mg/L), polyvynylpirrolidone (300 mg/L) and L-glutathione (50 mg/L) in two consecutive subculture cycles. Interaction between genotypes and antioxidants was significant in both cycles. All genotypes showed good shoot formation, shoot vigor and color, except in PI 88652 which had less shoot development in both the presence and absence of the antioxidants tested. PI 184794 displayed the highest shoot proliferation in the presence of citric acid, and UNK R65P35 produced more shoots per explant in the 2nd subculture. For S. sinense (PI 29109), in both subcultures, most shoots were observed in the presence of polyvynylpirrolidone. Medium discoloration due to phenolic secretion was reduced in the presence of citric acid and polyvynylpirrolidone. The type of secreted phenolic compounds differed with genotype as the Principal Component Analysis of cultivation media separated PI 88652 from PI 29109 and UKN R65P35. Phenolic compounds varied in composition and were secreted at various levels as a function of genotype and antioxidant type. Loadings plots indicated the genotype and antioxidant separations were broadly driven by flavonoid compounds.

Discrimination of toxic ingredient between raw and processed Pinellia ternata by UPLC/Q-TOF-MS/MS with principal component analysis and T-test

2019 ◽  
Vol 11 (2) ◽  
pp. 200-208 ◽  
Author(s):  
Xing-ying Zhai ◽  
Ling Zhang ◽  
Bing-tao Li ◽  
Yu-lin Feng ◽  
Guo-liang Xu ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Principal Component ◽  
Component Analysis ◽  
T Test ◽  
Pinellia Ternata ◽  
Tof Ms
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