scholarly journals Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS

Molecules ◽  
2016 ◽  
Vol 21 (11) ◽  
pp. 1471 ◽  
Author(s):  
Liangliang Liu ◽  
Juan Leng ◽  
Xiai Yang ◽  
Liping Liao ◽  
Yin Cen ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Rapid Screening ◽  
Radix Astragali ◽  
Screening And Identification

scholarly journals Rapid Screening and Identification of Antitumor Ingredients from the Mangrove Endophytic Fungus Using an Enzyme-Immobilized Magnetic Nanoparticulate System

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2255
Author(s):  
Nan Wei ◽  
Jun Zhao ◽  
Guimei Wu ◽  
Wenjuan Cao ◽  
Pei Luo ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Cyclic Peptides ◽  
High Performance ◽  
A549 Cells ◽  
Inhibitory Effect ◽  
Rapid Screening ◽  
Separation And Purification ◽  
Screening And Identification ◽  
Novel Strategy ◽  
Ligand Fishing

As a consequence of recent progression in biomedicine and nanotechnology, nanoparticle-based systems have evolved as a new method with extensive applications in responsive therapy, multimodal imaging, drug delivery and natural product separation. Meanwhile, the magnetic nanoparticulate system has aroused great interest for separation and purification because of its excellent magnetic properties. Phospholipase A2 (PLA2) is a highly expressed regulator to promote the growth of various cancers and is an ideal target to treat cancers. In this study, a novel strategy based on ligand–receptor interactions to discover novel PLA2 inhibitors was established, in which PLA2-functionalized Fe3O4@PLGA-PEG-NH2 magnetic nanoparticles were used as a supporting material combined with high-performance liquid chromatography–mass spectrometry, aiming to accelerate the discovery of novel PLA2 inhibitors from natural sources such as mangrove endophytic fungi. Under the optimized ligand fishing conditions, six target compounds were ultimately fished and identified to be cyclic peptides (1–3) and sterols (4–6), which compounds 1, 2 and 4–6 have well-documented cytotoxicities. Compound 3 exerted better inhibitory effect on A549 cells by experiment. In conclusion, PLA2-functionalized Fe3O4@PLGA-PEG-NH2 magnetic nanoparticles-based ligand fishing provided a feasible, selective and effective platform for the efficient screening and identification of antitumor components from natural products.

Rapid screening lipase inhibitors in licorice extract by using porcine pancreatic lipase immobilized Fe3O4 magnetic nanoparticles

2021 ◽  
Author(s):  
Fei Zeng ◽  
Wenxing Wu ◽  
Yiying Zhang ◽  
Xin Pan ◽  
Jinao Duan
Keyword(s):  
Magnetic Nanoparticles ◽  
Pancreatic Lipase ◽  
Rapid Screening ◽  
Porcine Pancreatic Lipase ◽  
Fe3o4 Magnetic Nanoparticles ◽  
Licorice Extract

Chalcones, a class of natural lipase inhibitors, have received substantial attention from researchers in recent years. Although many kinds of chalcones are typically distributed in G. inflata, there is a...

Magnetic nanoparticles-based lactate dehydrogenase microreactor as a drug discovery tool for rapid screening inhibitors from natural products

Talanta ◽  
2020 ◽  
Vol 209 ◽  
pp. 120554 ◽  
Author(s):  
Guorong Cheng ◽  
Zifeng Pi ◽  
Zhong Zheng ◽  
Shu Liu ◽  
Zhiqiang Liu ◽  
...  
Keyword(s):  
Natural Products ◽  
Drug Discovery ◽  
Magnetic Nanoparticles ◽  
Lactate Dehydrogenase ◽  
Rapid Screening

Human serum albumin-specific recognition of the natural herbal extract of Stryphnodendron polyphyllum through STD NMR, hyphenations and docking simulation studies

RSC Advances ◽  
2015 ◽  
Vol 5 (30) ◽  
pp. 23431-23442 ◽  
Author(s):  
Sheraz A. K. Tanoli ◽  
Nazish U. Tanoli ◽  
Tatiani M. Bondancia ◽  
Saman Usmani ◽  
Zaheer Ul-Haq ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Docking Simulation ◽  
Herbal Extract ◽  
Rapid Screening ◽  
Simulation Studies ◽  
Specific Recognition ◽  
Screening And Identification ◽  
Std Nmr

Over the last two decades, new and more advanced strategies that help in the rapid screening and identification of new ligands for a specific macromolecule have become an important domain.

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