scholarly journals Rapid Screening and Identification of Antitumor Ingredients from the Mangrove Endophytic Fungus Using an Enzyme-Immobilized Magnetic Nanoparticulate System

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2255
Author(s):  
Nan Wei ◽  
Jun Zhao ◽  
Guimei Wu ◽  
Wenjuan Cao ◽  
Pei Luo ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Cyclic Peptides ◽  
High Performance ◽  
A549 Cells ◽  
Inhibitory Effect ◽  
Rapid Screening ◽  
Separation And Purification ◽  
Screening And Identification ◽  
Novel Strategy ◽  
Ligand Fishing

As a consequence of recent progression in biomedicine and nanotechnology, nanoparticle-based systems have evolved as a new method with extensive applications in responsive therapy, multimodal imaging, drug delivery and natural product separation. Meanwhile, the magnetic nanoparticulate system has aroused great interest for separation and purification because of its excellent magnetic properties. Phospholipase A2 (PLA2) is a highly expressed regulator to promote the growth of various cancers and is an ideal target to treat cancers. In this study, a novel strategy based on ligand–receptor interactions to discover novel PLA2 inhibitors was established, in which PLA2-functionalized Fe3O4@PLGA-PEG-NH2 magnetic nanoparticles were used as a supporting material combined with high-performance liquid chromatography–mass spectrometry, aiming to accelerate the discovery of novel PLA2 inhibitors from natural sources such as mangrove endophytic fungi. Under the optimized ligand fishing conditions, six target compounds were ultimately fished and identified to be cyclic peptides (1–3) and sterols (4–6), which compounds 1, 2 and 4–6 have well-documented cytotoxicities. Compound 3 exerted better inhibitory effect on A549 cells by experiment. In conclusion, PLA2-functionalized Fe3O4@PLGA-PEG-NH2 magnetic nanoparticles-based ligand fishing provided a feasible, selective and effective platform for the efficient screening and identification of antitumor components from natural products.

scholarly journals Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS

Molecules ◽  
2016 ◽  
Vol 21 (11) ◽  
pp. 1471 ◽  
Author(s):  
Liangliang Liu ◽  
Juan Leng ◽  
Xiai Yang ◽  
Liping Liao ◽  
Yin Cen ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Rapid Screening ◽  
Radix Astragali ◽  
Screening And Identification

scholarly journals Rapid Characterization and Identification of Chemical Constituents in Gentiana radix before and after Wine-Processed by UHPLC-LTQ-Orbitrap MSn

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3222 ◽  
Author(s):  
Xin Lv ◽  
Jian-Zhi Sun ◽  
Shi-Zhao Xu ◽  
Qian Cai ◽  
Yu-Qiang Liu
Keyword(s):  
High Performance ◽  
Ion Trap ◽  
Mass Measurement ◽  
Chemical Constituents ◽  
Rapid Screening ◽  
Orbitrap Mass Spectrometry ◽  
Before And After ◽  
Screening And Identification ◽  
Peak Areas ◽  
Rapid Characterization

Gentiana radix is used in traditional Chinese medicine and has functions of clearing heat and drying dampness, as well as purging liver and gallbladder fire. A highly sensitive and effective strategy for rapid screening and identification of target constituents has been developed by using ultra high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap) in crude and wine-processed . Based on the accurate mass measurement (<5 ppm), retention times, and MS fragmentation ions, 52 constituents were unambiguously or tentatively characterized from Gentiana radix, including 21 iridoids, 11 flavonoids, 19 xanthones, and a triterpenoid. This study demonstrated that the established method could be a rapid, effective analytical tool for screening and characterization of compounds in the complex systems of . By comparing the structure and peak areas of chemical constituents in crude and wine-processed Gentiana radix, we found that some compounds in crude and wine-processed Gentiana radix were significantly different.

Rapid identification of polyphenols in Kudiezi injection with a practical technique of mass defect filter based on high-performance liquid chromatography coupled with linear ion trap/orbitrap mass spectrometry

2014 ◽  
Vol 6 (10) ◽  
pp. 3515-3523 ◽  
Author(s):  
Fang Wang ◽  
Jiayu Zhang ◽  
Peihua Yin ◽  
Zijian Wang ◽  
Luyan Dong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Ion Trap ◽  
Rapid Identification ◽  
Rapid Screening ◽  
Linear Ion Trap ◽  
Mass Defect ◽  
Orbitrap Mass Spectrometry ◽  
Screening And Identification ◽  
Mass Defect Filter

A practical methodology was developed for the rapid screening and identification of serial components in Kudiezi injection using a combination of LTQ-Orbitrap and MDF technology.

Rapid screening and analysis of alcohol dehydrogenase binders from Glycyrrhiza uralensis root extract using functionalized magnetic nanoparticles coupled with HPLC−MS/MS

2013 ◽  
Vol 91 (11) ◽  
pp. 1147-1154 ◽  
Author(s):  
Miao Chen ◽  
Liangliang Liu ◽  
Xiaoqing Chen
Keyword(s):  
Magnetic Nanoparticles ◽  
Alcohol Dehydrogenase ◽  
High Performance ◽  
Relative Activity ◽  
Binding Activity ◽  
Glycyrrhiza Uralensis ◽  
Rapid Screening ◽  
Root Extract ◽  
Covalent Bonds ◽  
Functionalized Magnetic Nanoparticles

Alcohol dehydrogenase (ADH) is a key enzyme that converts ethanol into acetaldehyde. Its binders could be used to efficiently treat human toxic alcohol poisoning and suppress the acetaldehyde accumulation in alcohol hypersensitive alcoholics. In the present study, a new assay based on ADH-functionalized magnetic nanoparticles coupled with high-performance liquid chromatography − tandem mass spectrometry (HPLC−MS/MS) was developed for the rapid screening and identification of ADH binders. ADH was immobilized on silica-coated Fe3O4 magnetic nanoparticles via covalent bonds and the synthesized nanoparticles were characterized by TEM, XRD, FT-IR, and VSM. The amount of bound ADH onto magnetic nanoparticles was 80.6 μg/mg. The optimum temperature and pH for the enzymatic activity of immobilized ADH were 25 °C and 7.0, respectively. The relative activity of immobilized ADH remained 68.14% after 10 times of recycle, which exhibited good durability. Nine compounds with ADH-binding activity were screened and identified in Glycyrrhiza uralensis root extracts, among which seven compounds were first screened and identified. Experimental results proved that the proposed method could rapidly screen ADH binders from complex mixtures.

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