scholarly journals Enhancement of Palmarumycin C12 and C13 Production in Liquid Culture of the Endophytic Fungus Berkleasmium sp. Dzf12 by Oligosaccharides from Its Host Plant Dioscorea zingiberensis

Molecules ◽  
2012 ◽  
Vol 17 (4) ◽  
pp. 3761-3773 ◽  
Author(s):  
Yan Li ◽  
Tijiang Shan ◽  
Yan Mou ◽  
Peiqin Li ◽  
Jianglin Zhao ◽  
...  
Keyword(s):  
Host Plant ◽  
Endophytic Fungus ◽  
Liquid Culture ◽  
Dioscorea Zingiberensis

scholarly journals Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Culture by Oligosaccharide Elicitor from its Endophytic Fungus Fusarium oxysporum Dzf17

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401
Author(s):  
Ruifen Zhang ◽  
Peiqin Li ◽  
Lijian Xu ◽  
Yuanquan Chen ◽  
Peng Sui ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Fusarium Oxysporum ◽  
Cell Suspension Culture ◽  
Endophytic Fungus ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Fungal Cell ◽  
Partial Acid Hydrolysis ◽  
Dioscorea Zingiberensis

Diosgenin accumulation in cell suspension cultures of Dioscorea zingiberensis C. H. Wright was enhanced by treatment with saccharide elicitors from its endophytic fungus Fusarium oxysporum isolate Dzf17. The crude oligosaccharide was prepared by partial acid hydrolysis of the isolated Dzf17 fungal cell wall fragments. Optimal elicitation of diosgenin production by the isolated Dzf17 oligosaccharide in cell suspension culture was achieved when the oligosaccharide was added to the medium at a concentration of 30 mg/L after 16-day's continuous cell suspension culture, and the cells were cultured for another 8 days before harvesting. By using these optimal conditions, the diosgenin yield of the cultured cells reached its maximum of 5.25 mg/L, which was over a three-fold increase.

Performance of Pinus contorta inoculated with two strains of root endophytic fungus, Phialocephala fortinii: effects of synthesis system and glucose concentration

1998 ◽  
Vol 76 (7) ◽  
pp. 1205-1213 ◽  
Author(s):  
Ari Jumpponen ◽  
James M Trappe
Keyword(s):  
Host Plant ◽  
Glucose Concentration ◽  
Endophytic Fungus ◽  
Culture System ◽  
Pinus Contorta ◽  
Ecological Role ◽  
Phialocephala Fortinii ◽  
Aseptic Culture ◽  
Foliar Nutrient ◽  
Host Fungus

Melanized, root-colonizing fungi are ubiquitous. Their ecological role, however, is poorly understood, and results of studies of associations between these fungi and their potential host plants are controversial. The culture system under which the association is studied may also affect the host-fungus interaction. Two experiments on the association between Pinus contorta Dougl. ex Loud., and a root-inhabiting endophytic fungus, Phialocephala fortinii Wang & Wilcox, were conducted to study the host response to inoculation. First, Pinus contorta seedlings were inoculated with two strains of Phialocephala fortinii and grown under aseptic conditions with five levels of glucose in the medium. Multiple linear regression analysis was employed to study the effects of inoculation and glucose concentration. Second, the same two strains of Phialocephala fortinii were inoculated on Pinus contorta seedlings in open pot cultures. Inoculation resulted in substantial increase in all biomass components of the host plant in the aseptic culture system. Total biomass, for example, was increased approximately 60 and 90% when seedlings were inoculated with strain 1 and strain 2, respectively. No seedling mortality was observed following fungal inoculation after 6 months of incubation. Inoculation increased host biomass with increasing glucose concentration, while glucose concentration did not significantly affect host biomass when no inoculum was added. Inoculation lowered foliar nitrogen and phosphorus concentrations. The effect of glucose concentration on the foliar nutrient concentrations varied between the two strains. In the open pot cultures, inoculation did not affect biomass or foliar nutrient concentration. We hypothesise that the observed increases in host growth in the aseptic culture system are due to fungal respiration in a closed culture system, the carbohydrates made available to the host plant by the fungus, or, most likely, to a combination of both factors. The ecological role of Phialocephala fortinii and the validity of aseptic culture assays are discussed.Key words: dark-septate endophytes, deuteromycetes, fungi, host-fungus associations, root endophytes, symbiosis.

scholarly journals Isolation and Charecterisation of Seaweed Endophytic Fungi as an Efficient Phosphate Solubiizers

2019 ◽  
Vol 16 (1) ◽  
pp. 33-39 ◽  
Author(s):  
A. Noorjahan ◽  
B. Aiyamperumal ◽  
P. Anantharaman
Keyword(s):  
Enzyme Activity ◽  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Liquid Culture ◽  
Penicillium Oxalicum ◽  
Chemical Fertilizer ◽  
Alternate Source ◽  
Aspergillus Sp ◽  
Phosphate Solubilizing ◽  
Solubilizing Activity

Phosphate-solubilizing fungi (PSF) generally enhance the availablility of phosphorus (P) released from soil, which contributes to plants' P requirement, especially in P-limiting regions. In this study we isolated endophytic fungi from seaweeds and screened for phosphate solubilizng in both solid and liquid culture and estimated the solubilizing index and enzyme activity. Six fungus of Penicillium oxalicum, P.citrinums and Aspergillus sp. shows maximum phosphate solubilizing activity. Hence Seaweed endophytic fungus isolated from chlorophyceae express as an alternate source to replace chemical fertilizer.

scholarly journals Role of Sulphate Transporter (PiSulT) of Endophytic Fungus Serendipita indica in Plant Growth and Development

2020 ◽  
Author(s):  
Om Prakash Narayan ◽  
Nidhi Verma ◽  
Abhimanyu Jogawat ◽  
Meenakshi Dua ◽  
Atul Kumar Johri
Keyword(s):  
Plant Growth ◽  
Host Plant ◽  
Endophytic Fungus ◽  
Growth And Development ◽  
Crop Production ◽  
Major Facilitator Superfamily ◽  
Plant Growth And Development ◽  
Plant Metabolites ◽  
High Affinity ◽  
Growth Development

ABSTRACTSulfur is an important macronutrient required for the growth, development of plants and is a key component of many metabolic pathways. We have functionally characterized a high-affinity sulphate transporter (PiSulT) from an endophytic fungus Serendipita indica. The PiSulT belongs to the major facilitator superfamily (MFS) of membrane transporter. The PiSulT functionally complements the yeast sulphate transporter mutant HK14. PiSulT is a high-affinity sulphate transporter, having Km 15μM. We found enhanced expression of PiSulT in external fungal hyphae which helps the fungus in the acquisition of sulphate from the soil. When knockdown (KD)-PiSulT-P.indica colonized with the plant, it results in an 8-fold reduction in the transfer of sulphate to the colonized plants as compared to the plants colonized with the WT S. indica, which suggests that PiSulT is playing a role in sulphate transfer from soil to host plant. Further, plants colonized with the WT S. indica were found to be healthy in comparison to the plants colonized with the KD-PiSulT-P.indica. Additionally, S. indica colonization provides a positive effect on total sulfur content and on plant metabolites like sulfate ions and glutathione, particularly under low sulphate condition. We observed that the expression of sulfur assimilation pathway genes of S. indica and plant is dependent on the availability of sulphate and on the colonization with the plant. Our study highlights the importance of PiSulT in the improvement of sulfur nutrition of host plant particularly under low sulphate condition and in plant growth development. This study will open new vistas to use S. indica as a bio-fertilizer in the sulphate deficient field to improve crop production.One-Sentence SummaryHigh-affinity sulphate transporter of Serendipita indica (PiSulT) transfer sulphate from soil to plant under low sulphate condition and improve plant growth and development.

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