scholarly journals Enhancement of Diepoxin ζ Production by Yeast Extract and Its Fractions in Liquid Culture of Berkleasmium-Like Endophytic Fungus Dzf12 from Dioscorea zingiberensis

Molecules ◽  
2011 ◽  
Vol 16 (1) ◽  
pp. 847-856 ◽  
Author(s):  
Jianglin Zhao ◽  
Bingbing Zheng ◽  
Yan Li ◽  
Tijiang Shan ◽  
Yan Mou ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Endophytic Fungus ◽  
Liquid Culture ◽  
Dioscorea Zingiberensis ◽  
Diepoxin Ζ

scholarly journals Spirobisnaphthalenes from the Endophytic Fungus Dzf12 of Dioscorea zingiberensis and Their Antimicrobial Activities

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Xiaoyue Cai ◽  
Tijiang Shan ◽  
Peiqin Li ◽  
Yongfu Huang ◽  
Lijian Xu ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Endophytic Fungus ◽  
Antimicrobial Activities ◽  
Spectrometric Analysis ◽  
Antifungal Activities ◽  
Dioscorea Zingiberensis ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal ◽  
Diepoxin Ζ

Five spirobisnaphthalenes, namely palmarumycin CP17 (1), diepoxin κ (2), diepoxin η (3), diepoxin ζ (4), and diepoxin γ (5), were isolated from the endophytic fungus Dzf12 associated with the medicinal plant Dioscorea zingiberensis C. H. Wright. Their structures were identified by physicochemical and spectrometric analysis. Among these spirobisnaphthalenes, 2 was found to have antibacterial activity, and the mixture of 3 and 4 was detected to have both antibacterial and antifungal activities.

Production and characterization of biomass and exopolysaccharides obtained in submerged culture under different initial pHs used in the cultivation of Colletotrichum gloeosporioides and Rhizopus stolonifer

2020 ◽  
pp. 628
Author(s):  
Juan Diego Valenzuela Cobos ◽  
René Oscar Rodríguez-Grimón ◽  
Ana Grijalva-Endara ◽  
Raúl Marcillo-Vallejo ◽  
Onay Adonys Mercader-Camejo
Keyword(s):  
Yeast Extract ◽  
Submerged Culture ◽  
Liquid Culture ◽  
Colletotrichum Gloeosporioides ◽  
Culture Media ◽  
Mycelial Biomass ◽  
Rhizopus Stolonifer

Colletotrichum gloeosporioides (GC003) and Rhizopus stolonifer (RS001) were cultivated in two different liquid culture media: LC1 (glucose 40 g L-1, yeast extract 3 g L-1 and tryptone peptone 2 g L-1) and LC2 (glucose 40 g L-1, yeast extract 3 g L-1 and tryptone peptone 10 g L-1) for the production of mycelial biomass and exopolysaccharides (EPS). By using the liquid culture (LC2) under pH of 4.5 presented the highest biomass content (15.73 g L-1) in the propagation of Rhizopus stolonifer. The highest production of exopolysaccharides (1.74 g L-1) was obtained by the liquid culture (LC2) under pH of 4.5 in the cultivation of Colletotrichum gloeosporioides. The results presented that the production of biomass and exopolysaccharides (EPS) is directly related with the pHs values and the strain used in the cultivation.

scholarly journals Sorption of Zinc by exopolysaccharides produced by liquid media of phytopatogenic fungi

2021 ◽  
Vol 26 (1) ◽  
pp. 2312-2317
Author(s):  
JUAN DIEGO VALENZUELA-COBOS ◽  
ANA GRIJALVA-ENDARA
Keyword(s):  
Yeast Extract ◽  
Liquid Culture ◽  
Culture Medium ◽  
Colletotrichum Gloeosporioides ◽  
Culture Media ◽  
Phytopathogenic Fungi ◽  
Mycelial Biomass ◽  
Rhizopus Stolonifer ◽  
Liquid Media ◽  
Liquid Culture Medium

Phytopathogenic fungi such as: Colletotrichum gloeosporioides and Rhizopus stolonifer were cultivated in three different liquid culture media: LCC (glucose 40 g L-1 , yeast extract 3 g L-1 ), LC2 (glucose 40 g L-1 , yeast extract 3 g L-1 and tryptone peptone 2 g L-1 ) and LC3 (glucose 40 g L-1 , yeast extract 3 g L-1 and tryptone peptone 10 g L-1 ) under pH of 5.5 for the production of mycelial biomass and exopolysaccharides (EPS). The liquid culture medium (LC3) used in cultivation of Colletotrichum gloeosporioides showed the highest production of biomass (15.40 g L-1 ) and exopolysaccharides (3.40 g L-1 ). Exopolysaccharides (EPS) obtained from the liquid culture medium (LC3) of Colletotrichum gloeosporioides presented the highest absorption content of Zinc (56 mg g-1 ). The results presented that the exopolysaccharides (EPS) produced by Colletotrichum gloeosporioides showed the greatest biosorbent capacity of Zinc (Zn) using the culture medium with the highest amount of tryptone peptone.

scholarly journals Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Culture by Oligosaccharide Elicitor from its Endophytic Fungus Fusarium oxysporum Dzf17

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401
Author(s):  
Ruifen Zhang ◽  
Peiqin Li ◽  
Lijian Xu ◽  
Yuanquan Chen ◽  
Peng Sui ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Fusarium Oxysporum ◽  
Cell Suspension Culture ◽  
Endophytic Fungus ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Fungal Cell ◽  
Partial Acid Hydrolysis ◽  
Dioscorea Zingiberensis

Diosgenin accumulation in cell suspension cultures of Dioscorea zingiberensis C. H. Wright was enhanced by treatment with saccharide elicitors from its endophytic fungus Fusarium oxysporum isolate Dzf17. The crude oligosaccharide was prepared by partial acid hydrolysis of the isolated Dzf17 fungal cell wall fragments. Optimal elicitation of diosgenin production by the isolated Dzf17 oligosaccharide in cell suspension culture was achieved when the oligosaccharide was added to the medium at a concentration of 30 mg/L after 16-day's continuous cell suspension culture, and the cells were cultured for another 8 days before harvesting. By using these optimal conditions, the diosgenin yield of the cultured cells reached its maximum of 5.25 mg/L, which was over a three-fold increase.

Morphogenesis and wall chemistry of the yeast, "intermediate," and hyphal phases of the dimorphic fungus, Mycotypha poitrasii

1980 ◽  
Vol 26 (1) ◽  
pp. 36-49 ◽  
Author(s):  
Garry T. Cole ◽  
Takashi Sekiya ◽  
Reiko Kasai ◽  
Yoshinori Nozawa
Keyword(s):  
Cell Wall ◽  
Yeast Extract ◽  
Liquid Culture ◽  
Developmental Phase ◽  
Dimorphic Fungus ◽  
Comparative Analyses ◽  
Cell Wall Chemistry ◽  
Mycelial Yeast ◽  
Intermediate Cells

When Mycotypha poitrasii(Zygomycetes) is grown under standard conditions in liquid culture containing 1% polypeptone, 0.5% yeast extract, and variable glucose concentrations (0–6%), it displays mycelial–yeast conversion. "Intermediate" cells, isolated from cultures containing 2% glucose, are considered to represent a developmental phase in the process of morphogenesis. Distinct differences in the morphology and wall chemistry of the intermediate cells were demonstrated when compared to the yeast and hyphal forms. It is suggested that the trends evident from these comparative analyses reflect relationships between the alterations in cell wall chemistry and morphogenetic aspects of dimorphism.

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