scholarly journals Isolation and Charecterisation of Seaweed Endophytic Fungi as an Efficient Phosphate Solubiizers

2019 ◽  
Vol 16 (1) ◽  
pp. 33-39 ◽  
Author(s):  
A. Noorjahan ◽  
B. Aiyamperumal ◽  
P. Anantharaman
Keyword(s):  
Enzyme Activity ◽  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Liquid Culture ◽  
Penicillium Oxalicum ◽  
Chemical Fertilizer ◽  
Alternate Source ◽  
Aspergillus Sp ◽  
Phosphate Solubilizing ◽  
Solubilizing Activity

Phosphate-solubilizing fungi (PSF) generally enhance the availablility of phosphorus (P) released from soil, which contributes to plants' P requirement, especially in P-limiting regions. In this study we isolated endophytic fungi from seaweeds and screened for phosphate solubilizng in both solid and liquid culture and estimated the solubilizing index and enzyme activity. Six fungus of Penicillium oxalicum, P.citrinums and Aspergillus sp. shows maximum phosphate solubilizing activity. Hence Seaweed endophytic fungus isolated from chlorophyceae express as an alternate source to replace chemical fertilizer.

scholarly journals Identification of phosphate-solubilizing microorganisms and determination of their phosphate-solubilizing activity and growth-promoting capability

BioResources ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. 2560-2578 ◽  
Author(s):  
Ying-Ying Wang ◽  
Pei-Shan Li ◽  
Bi-Xian Zhang ◽  
Yan-Ping Wang ◽  
Jing Meng ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Phosphorus Content ◽  
Ph Value ◽  
Inverse Correlation ◽  
Penicillium Oxalicum ◽  
Fermentation Time ◽  
Soluble Phosphorus ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Solubilizing Activity

Phosphate-solubilizing microorganisms have been considered as a novel alternative approach to provide phosphate fertilizers that promote plant growth. In this study, three strains were isolated and identified as Penicillium oxalicum FJG21, Penicillium oxalicum FJQ5, and Bacillus subtilis BPM12, with a relatively high phosphate-solubilizing activity. Various phosphate sources were investigated, and Ca3(PO4)2 was identified as the effective phosphate source. Factors governing the phosphate-solubilizing activity of the strains included carbon and nitrogen sources, initial pH, and fermentation time. A high soluble phosphorus content was achieved with 529.0 μg·mL-1, 514.0 μg·mL-1, and 330.7 μg·mL-1 for Penicillium oxalicum FJG21, Penicillium oxalicum FJQ5, and Bacillus subtilis BPM12, respectively. An inverse correlation of the quantity of soluble phosphorus content and the pH value of the medium was observed. In addition, Bacillus subtilis BPM12 displayed a prominent capability of producing indole acetic acid. Penicillium oxalicum FJG21 and Penicillium oxalicum FJQ5 exhibited high cellulase activities. These phosphate-solubilizing microorganisms with good phosphate-solubilizing capability and growth-promoting ability are the promising strains for agricultural utilization.

scholarly journals Optimization of process parameters for production of antimicrobial metabolites by an endophytic fungus Aspergillus sp. CPR5 isolated from Calotropis procera root.

2017 ◽  
Vol 10 (4) ◽  
pp. 225 ◽  
Author(s):  
Shiv Kumar Verma ◽  
Moti Lal ◽  
Mira Debnath
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Crude Extract ◽  
Endophytic Fungus ◽  
Sclerotium Rolfsii ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Calotropis Procera ◽  
Aspergillus Sp ◽  
Antimicrobial Metabolites

Objective:To study the antimicrobial activity of crud ethylacetate  extract from endophytic fungus in Calotropis procera root.Methods:Endophytic fungus was screened for production of antimicrobial metablites.fermentation was carried out in 500 ml Erlenmeyer flask. Disc diffusion method was used to test antimicrobial activity of crude extract using chloramphenicol sulphate and Flucnazole as positive controleRessults: A total of fourteen Endophytic fungi were isolated (CPR1- CPR14). Among these fourteen isolates, CPR5 was found to show maximum antimicrobial activity, in compare to other isolates, against gram positive, gram negative bacteria and fungi. The antimicrobial activity was tested against Escherichia coli. Pseudomonas aeroginosa, Bacillus subtilis, Staphylococcus aureus, Ralstonia solanacearum, Xanthomonas oryzae, Penicillium chrysogenum, Candida albicans, Phoma exigua, Sclerotium rolfsii and Sclerotinia scleratiourum. Minimum inhibitory concentration (MIC) of crude extract against test microorganisms was determined. Fungus was identified as aspergillus sp. Prduction parameters (Temprature, pH, Carbon source, Nitrgen surce, Sdium Chlride concentration) were optimized.Conclusion:  Crud  extract produced by the isolated endophytic fungus could be an important source of broad spectrum antimicrobial metabolites. Keywords: Endophytic fungi, Antimicrobial metabolites, Process optimization, Calotropis procera, Inhibition zone.

scholarly journals Bioactive Metabolites from the Endophytic Fungus Aspergillus sp. SPH2

2021 ◽  
Vol 7 (2) ◽  
pp. 109
Author(s):  
Viridiana Morales-Sánchez ◽  
Carmen E. Díaz ◽  
Elena Trujillo ◽  
Sonia A. Olmeda ◽  
Felix Valcarcel ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Plant Pathogens ◽  
Insect Pests ◽  
Ethyl Acetate Extract ◽  
Rhopalosiphum Padi ◽  
Fungal Growth ◽  
Bioactive Metabolites ◽  
Endemic Plant ◽  
Antifungal Effects ◽  
Aspergillus Sp

In the current study, an ethyl acetate extract from the endophytic fungus Aspergillus sp. SPH2 isolated from the stem parts of the endemic plant Bethencourtia palmensis was screened for its biocontrol properties against plant pathogens (Fusarium moniliforme, Alternaria alternata, and Botrytis cinerea), insect pests (Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi), plant parasites (Meloidogyne javanica), and ticks (Hyalomma lusitanicum). SPH2 gave extracts with strong fungicidal and ixodicidal effects at different fermentation times. The bioguided isolation of these extracts gave compounds 1–3. Mellein (1) showed strong ixodicidal effects and was also fungicidal. This is the first report on the ixodicidal effects of 1. Neoaspergillic acid (2) showed potent antifungal effects. Compound 2 appeared during the exponential phase of the fungal growth while neohydroxyaspergillic acid (3) appeared during the stationary phase, suggesting that 2 is the biosynthetic precursor of 3. The mycotoxin ochratoxin A was not detected under the fermentation conditions used in this work. Therefore, SPH2 could be a potential biotechnological tool for the production of ixodicidal extracts rich in mellein.

Asperpyrone, a rare α-pyrone metabolite produced by an endophytic fungus Aspergillus sp. isolated from Garicinia smeathmannii (Planch. & Triana) Oliv.

2021 ◽  
pp. 1-8
Author(s):  
Jean-Bosco Jouda ◽  
Emmanuel Mfotie Njoya ◽  
Ruland Tchuinkeu Nguengang ◽  
Virginie Flaure Tsague Tankeu ◽  
Hans-Georg Stammler ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Aspergillus Sp

scholarly journals Aspergiloid I, an unprecedented spirolactone norditerpenoid from the plant-derived endophytic fungus Aspergillus sp. YXf3

2014 ◽  
Vol 10 ◽  
pp. 2677-2682 ◽  
Author(s):  
Zhi Kai Guo ◽  
Rong Wang ◽  
Wei Huang ◽  
Xiao Nian Li ◽  
Rong Jiang ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Spectroscopic Data ◽  
Biosynthetic Pathway ◽  
Culture Broth ◽  
Ring System ◽  
X Ray Diffraction ◽  
X Ray ◽  
Anti Oxidant ◽  
Inhibitory Activities ◽  
Aspergillus Sp

An unusual C18 norditerpenoid, aspergiloid I (1), was isolated from the culture broth of Aspergillus sp. YXf3, an endophytic fungus derived from Ginkgo biloba. Its structure was unambiguously established by analysis of HRMS–ESI and spectroscopic data, and the absolute configuration was determined by low-temperature (100 K) single crystal X-ray diffraction with Cu Kα radiation. This compound is structurally characterized by a new carbon skeleton with an unprecedented 6/5/6 tricyclic ring system bearing an α,β-unsaturated spirolactone moiety in ring B, and represents a new subclass of norditerpenoid, the skeleton of which is named aspergilane. The hypothetical biosynthetic pathway for 1 was also proposed. The cytotoxic, antimicrobial, anti-oxidant and enzyme inhibitory activities of 1 were evaluated.

scholarly journals Asperlones A and B, Dinaphthalenone Derivatives from a Mangrove Endophytic Fungus Aspergillus sp. 16-5C

Marine Drugs ◽  
2015 ◽  
Vol 13 (1) ◽  
pp. 366-378 ◽  
Author(s):  
Ze'en Xiao ◽  
Shao'e Lin ◽  
Chunbing Tan ◽  
Yongjun Lu ◽  
Lei He ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Mangrove Endophytic Fungus ◽  
Aspergillus Sp

scholarly journals Isolation of Endophytic Fungi QPS 05 from Quercus phillyraeoides A. Gray and Its Potential for α-Glucosidase Inhibitory Activity

2018 ◽  
Vol 20 (1) ◽  
pp. 1-7
Author(s):  
Anastasia Wheni Indrianingsih ◽  
Amalia Indah Prihantini ◽  
Sanro Tachibana
Keyword(s):  
Fatty Acids ◽  
Endophytic Fungi ◽  
Inhibitory Activity ◽  
Endophytic Fungus ◽  
Inhibitory Concentration ◽  
Life Cycles ◽  
Ic50 Values ◽  
Quercus Phillyraeoides ◽  
Alternaria Sp ◽  
Harmful Effects

AbstractEndophytic fungi are the microorganisms that spend all or part of their life cycles within plant tissue without causing harmful effects on the plant. In this study, 14 endophytic fungus from Quercus phillyraeoides A. Gray were isolated. Alternaria sp. QPS 05, an endophytic fungi which was isolated from the stem of Q. phillyraeoides A. Gray showed the highest α-glucosidase inhibitory activity. Further separation of ethyl acetate extract from the fungus led to the isolation of active substance from hexane-soluble fraction which give fatty acids mixture consist of palmitic acid, oleic acid, linoleic acid and linolenic acid (1) strong inhibitory activity against α-glucosidase. Isolated fatty acids (1) had inhibitory concentration (IC50) values against Saccharomyces cerevisiae was 12.10 μg/mL. The results of the present study showed that endophytic fungus from Alternaria sp. QPS 05 potentially contained a rich source of natural antidiabetic medicine.

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