scholarly journals Effectiveness of Phage-Based Inhibition of Listeria monocytogenes in Food Products and Food Processing Environments

2020 ◽  
Vol 8 (11) ◽  
pp. 1764
Author(s):  
Iwona Kawacka ◽  
Agnieszka Olejnik-Schmidt ◽  
Marcin Schmidt ◽  
Anna Sip
Keyword(s):  
Listeria Monocytogenes ◽  
Food Processing ◽  
Food Industry ◽  
Storage Temperature ◽  
Bacterial Pathogen ◽  
Food Contamination ◽  
Food Products ◽  
Pathogen Resistance ◽  
Food Matrix ◽  
Matrix Properties

Providing safe products and compliance of legal requirements is still a great challenge for food manufacturers regarding microbiological safety, especially in the context of Listeria monocytogenes food contamination. L. monocytogenes is a human pathogen, which, due to the ability of survival and proliferation in preservation conditions such as high salinity, acidity and refrigeration temperatures, is a significant threat to the food industry. Novel methods of elimination of the bacterial pathogen in food products and food processing environments are required. Among emerging technologies, one of the very promising solutions is using bacteriophages as natural control agents. This review focus on the major aspects of phage-based inhibition of L. monocytogenes in aspects of food safety. We describe an overview of foods and technological factors influencing the efficacy of phage use in biocontrol of L. monocytogenes. The most noteworthy are food matrix properties, phage concentration and stability, the time of phage application and product storage temperature. The combined methods, phage immobilization (active packing), pathogen resistance to phages and legislation aspects of antilisterial bacteriophage use in the food industry are also discussed.

Effect of Time, Temperature and Transport Media on the Recovery of Listeria monocytogenes from Environmental Swabs

2020 ◽  
Author(s):  
Diana Stewart ◽  
Yadwinder Singh Rana ◽  
Kaiping Deng ◽  
Geethaanjali Vijayakumar ◽  
Lanlan Yin ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Logistic Regression ◽  
Listeria Monocytogenes ◽  
Sodium Hypochlorite ◽  
Food Processing ◽  
Storage Time ◽  
Cheese Whey ◽  
Storage Temperature ◽  
Food Matrix ◽  
Processing Facility

Environmental monitoring for Listeria monocytogenes in food processing environments is key for ensuring the safety of ready-to-eat foods. For sampling, swabs are often hydrated with a wetting or transport medium which may contain neutralizers and other ingredients. After swabbing the environment, the swabs may then be transported or shipped cold to an off-site laboratory for testing, ideally within 48 h. Extended shipping times may subject the pathogen to increased temperatures in the presence of the wetting medium, organics, and other chemicals from the processing facility which may confound detection. This study evaluated growth and detection of L. monocytogenes on stainless steel exposed to either buffer or sodium hypochlorite prior to drying. Swabs were rehydrated with Butterfield’s Phosphate Buffer, Neutralizing Buffer, Letheen Broth or Dey-Engley Neutralizing Broth prior to swabbing. Swabs were stored in the presence of no added food, cheese whey or ice cream under both optimal (4°) and sub-optimal (15°C) temperatures for up to 72 h. Overall, there was no growth of L. monocytogenes at 4°C through 72 h storage, though enrichment from these swabs was dependent on the presence and type of food matrix. Pathogen growth during storage at 15°C was more variable and depended on both the food matrix and transport media used, with Dey-Engley and Letheen Broth allowing for the highest population increases. Overall, more enrichments resulting in L. monocytogenes detections were observed when using Letheen Broth and Neutralizing Buffer than Dey-Engley which resulted in fewer detections at 15°C. Logistic regression and Cochran-Mantel-Haenszel (CMH) analyses determined that storage temperature, transport media, and food matrix all significantly affected detection of L. monocytogenes , while storage time did not have a clear effect on recovery from swabs.

scholarly journals Modelling the potential role of media campaigns on the control of Listeriosis

2021 ◽  
Vol 18 (6) ◽  
pp. 7580-7601
Author(s):  
C. W. Chukwu ◽  
◽  
F. Nyabadza ◽  
Fatmawati ◽  
◽  
...  
Keyword(s):  
Removal Rate ◽  
Bacterial Pathogen ◽  
Food Contamination ◽  
Food Products ◽  
Model Parameters ◽  
Media Campaigns ◽  
Awareness Campaigns ◽  
Contaminated Food ◽  
The Impact

<abstract><p>Human Listeria infection is a food-borne disease caused by the consumption of contaminated food products by the bacterial pathogen, Listeria. In this paper, we propose a mathematical model to analyze the impact of media campaigns on the spread and control of Listeriosis. The model exhibited three equilibria namely; disease-free, Listeria-free and endemic equilibria. The food contamination threshold is determined and the local stability analyses of the model is discussed. Sensitivity analysis is done to determine the model parameters that most affect the severity of the disease. Numerical simulations were carried out to assess the role of media campaigns on the Listeriosis spread. The results show that; an increase in the intensity of the media awareness campaigns, the removal rate of contaminated food products, a decrease in the contact rate of Listeria by humans results in fewer humans getting infected, thus leading to the disease eradication. An increase in the depletion of media awareness campaigns results in more humans being infected with Listeriosis. These findings may significantly impact policy and decision-making in the control of Listeriosis disease.</p></abstract>

scholarly journals Personalised nutritional powder for elderly developed in OPTIFEL European Project

2016 ◽  
Vol 73 (2) ◽  
pp. 149 ◽  
Author(s):  
Monica TRIF ◽  
Lavinia MURESAN ◽  
Malte BETHKE
Keyword(s):  
Food Industry ◽  
Food Products ◽  
Food Matrix ◽  
Novel Food ◽  
European Project ◽  
Key Players ◽  
Elderly Populations ◽  
Fluid Bed ◽  
At Home

A personalized nutritional powder for elderly containing minerals and vitamins was developed by CENTIV GmbH for further applications in the FP7 European OFTIFEL project by designing and processing of novel food products such as nutrient dense smoothie products. The amount of the powder used for each person, in each product, is calculated using a food calculator, which was developed within the same FP7 European OFTIFEL project, named OPTIFEL Personalised Nutritional Calculator.The personalized nutritional powder consisting of several minerals and vitamins, has been agglomerated in a Fluid Bed Dryer using maltodextrin as carrier, in order to obtain a higher solubility, homogenization of all the nutrients, a desired supplement with a neutral taste, free of color and which can be added in any drinks or food matrix for enrichment in a certain concentration. It will not influence the final taste and has great solubility.Developing innovative food and services tailored to elderly populations is a new challenge for key players involved in food industry and catering. The population targeted by the OPTIFEL project is elderly cooking at home or making use of meal-on-wheels services for whom the project will develop food products adapted to their taste, habits, needs and constraints.

scholarly journals Potential Application of Essential Oils for Mitigation of Listeria monocytogenes in Meat and Poultry Products

2020 ◽  
Vol 7 ◽  
Author(s):  
Mojtaba Yousefi ◽  
Nasim Khorshidian ◽  
Hedayat Hosseini
Keyword(s):  
Listeria Monocytogenes ◽  
Essential Oils ◽  
Food Industry ◽  
Antimicrobial Properties ◽  
Food Products ◽  
Cell Permeability ◽  
Bioactive Components ◽  
Plant Essential Oils ◽  
Poultry Products ◽  
Safe Food

One of the most important challenges in the food industry is to provide healthy and safe food. Therefore, it is not possible to achieve this without different processes and the use of various additives. In order to improve safety and extend the shelf life of food products, various synthetic preservatives have been widely utilized by the food industry to prevent growth of spoilage and pathogenic microorganisms. On the other hand, consumers' preference to consume food products with natural additives induced food industries to use natural-based preservatives in their production. It has been observed that herbal extracts and their essential oils could be potentially considered as a replacement for chemical antimicrobials. Antimicrobial properties of plant essential oils are derived from some main bioactive components such as phenolic acids, terpenes, aldehydes, and flavonoids that are present in essential oils. Various mechanisms such as changing the fatty acid profile and structure of cell membranes and increasing the cell permeability as well as affecting membrane proteins and inhibition of functional properties of the cell wall are effective in antimicrobial activity of essential oils. Therefore, our objective is to revise the effect of various essential oils and their bioactive components against Listeria monocytogenes in meat and poultry products.

scholarly journals Modeling the Potential Role of Media Campaigns on the Control of Listeriosis

2020 ◽  
Author(s):  
C.W. Chukwu ◽  
F. Nyabadza
Keyword(s):  
Bacterial Pathogen ◽  
Food Contamination ◽  
Food Products ◽  
Model Parameters ◽  
Asymptotically Stable ◽  
Media Campaigns ◽  
Awareness Campaigns ◽  
The Impact ◽  
Disease Free

AbstractThe human Listeriosis infection is a food-borne disease caused by the consumption of food products contaminated by the bacterial pathogen, Listeria. In this paper, we propose a mathematical model to analyse the impact of media awareness campaigns on the spread and control of Listeriosis. The model equilibria are determined and the model has three equilibria namely; the disease-free, the Listeria-free, and the endemic equilibria. The food contamination constant (ℛf) is determined and the stability analysis shows that the disease-free steady state is locally asymptotically stable if ℛf < 1, the Listeria-free and endemic steady states are locally asymptotically stable whenever ℛf > 1. Sensitivity analysis is done to determine the model parameters that most affect the severity of the disease. Numerical simulations are carried out to determine the role of media awareness campaigns on the spread of the Listeriosis. The results show that an increase in the intensity of the media awareness campaigns and removal of contaminated food products, a decrease in the contact rate, results in fewer humans getting infected leading to disease eradication, while an increase in the depletion of media awareness campaigns results in more humans been infected with Listeriosis. These findings may significantly impact policy and decision making in the control of Listeriosis disease.

scholarly journals Complete Genome Sequences of 12 Isolates of Listeria monocytogenes Belonging to Serotypes 1/2a, 1/2b, and 4b Obtained from Food Products and Food-Processing Environments in Canada

2017 ◽  
Vol 5 (19) ◽  
Author(s):  
Walid Mottawea ◽  
Shu Chen ◽  
Saleema Saleh-Lakha ◽  
Sebastien Belanger ◽  
Dele Ogunremi
Keyword(s):  
Listeria Monocytogenes ◽  
Food Processing ◽  
Complete Genome ◽  
Food Products ◽  
Foodborne Illness ◽  
Etiological Agent ◽  
Genome Sequences ◽  
Content Type ◽  
Common Serotypes

ABSTRACT Listeria monocytogenes is the etiological agent for an often fatal foodborne illness known as listeriosis. Here, we present the complete genome sequences of 12 L. monocytogenes isolates representing the three most common serotypes of this pathogen (1/2a, 1/2b, and 4b), collected in Canada from different food products and environmental sources.

Modeling the Growth of Listeria monocytogenes in Pasteurized White Asparagus

2007 ◽  
Vol 70 (3) ◽  
pp. 753-757 ◽  
Author(s):  
A. VALERO ◽  
E. CARRASCO ◽  
F. PÉREZ-RODRÍGUEZ ◽  
R. M. GARCÍA-GIMENO ◽  
G. ZURERA
Keyword(s):  
Listeria Monocytogenes ◽  
Food Processing ◽  
Goodness Of Fit ◽  
Microbial Population ◽  
Storage Temperature ◽  
Arrhenius Model ◽  
Microbial Risks ◽  
The Relationship ◽  
And Storage ◽  
Storage Temperatures

Growth of Listeria monocytogenes in pasteurized white asparagus was monitored at different storage temperatures (4, 10, 20, and 30°C). Among the main microbial kinetic parameters, growth rate (μ) per hour was calculated at each temperature using the Baranyi-Roberts model. L. monocytogenes was able to grow at all temperatures, although at 4°C only a slight increment of the microbial population was observed (∼1 log CFU/g) after 300 h of storage. Subsequently, two different secondary modeling approaches were proposed to study the relationship between μ and storage temperature: the Arrhenius and Ratkowsky models. Although both models properly described the data observed, smaller values of root mean square error (RMSE) and standard error of prediction (SEP) were obtained with the Ratkowsky model, providing a better goodness of fit (Ratkowsky model: RMSE = 0.010, SEP = 21.23%; Arrhenius model: RMSE = 0.026, SEP = 54.37%). The maximum population density (MPD) was calculated at each temperature studied. A clear dependence between MPD and temperature was found; lower temperatures produced lower values of MPD. This finding confirmed the Jameson effect, indicating that multiple hurdles in the food-processing chain plus lower temperatures reduced L. monocytogenes growth. Predicting the growth of L. monocytogenes along the food chain will help to reduce microbial risks associated with consumption of pasteurized white asparagus.

Listeria monocytogenes: biofilm formation and persistence in food-processing environments

Biofilms ◽  
2004 ◽  
Vol 1 (2) ◽  
pp. 107-121 ◽  
Author(s):  
T. Møretrø ◽  
S. Langsrud
Keyword(s):  
Listeria Monocytogenes ◽  
Food Processing ◽  
Food Industry ◽  
Single Cells ◽  
Processing Plant ◽  
Production Environment ◽  
Adhesive Properties ◽  
Free Living ◽  
Survival And Growth ◽  
Cleaning And Disinfection

Listeria monocytogenes is ubiquitous in nature and a major concern for the food industry, since it is the causal agent of the serious foodborne illness listeriosis. This organism can be introduced through many routes to food-processing environments and may become established on food-processing equipment. Subsequently, food products may become contaminated during processing. In addition, the bacterium can grow at refrigeration temperatures. Biofilms are regarded as important with respect to the survival and growth of microorganisms in the food industry. Microorganisms growing in biofilms are protected against cleaning and disinfection and are difficult to eradicate. Listeria monocytogenes may grow in biofilms that protect them against environmental stress and can be isolated from surfaces after cleaning and disinfection. For each individual food-processing plant, a limited number of clones of L. monocytogenes may become established and persist for years. Persistent strains adhere to surfaces and form biofilms more readily compared to sporadically found strains, suggesting that adherence to surfaces is important for survival and persistence of L. monocytogenes in food-processing environments. Listeria monocytogenes can adhere to all the materials commonly used in the food industry. In biofilms L. monocytogenes is significantly more resistant to disinfection than its free-living counterparts and thick, complex biofilms are more difficult to remove than adherent single cells of L. monocytogenes. Several novel approaches to avoid adhesion of L. monocytogenes have been proposed, but high costs, practical difficulties or resistance problems limit their practical use. Despite considerable research on the adhesive properties and resistance of L. monocytogenes enabling its survival in the food production environment, a final solution for avoiding establishment of the bacterium has not yet been found.

scholarly journals Aspects regarding the use of ultrasounds in the food industry

2021 ◽  
Vol 286 ◽  
pp. 04009
Author(s):  
Cristian Marian Sorică ◽  
Marian Vintilă ◽  
Laurentiu Vlădutoiu ◽  
Elena Sorică ◽  
Iulia Andreea Grigore ◽  
...  
Keyword(s):  
Food Processing ◽  
Food Industry ◽  
Food Products ◽  
Research Field ◽  
Technical Equipment ◽  
Horticultural Products

Ultrasound is a rapidly developping research field that is more and more used in the food domain, both for analysis and processing of food products. This technology has been successfully applied within food industry for various processes like freezing, filtration, drying, separation, emulsion, sterilization and extraction. The paper presents aspects regarding the mechanism and use of ultrasound in food processing, as well as some technical equipment that use ultrasound within a combined technology, for the decontamination of the external surfaces of some horticultural products.

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