scholarly journals Integrated Stirred-Tank Bioreactor with Internal Adsorption for the Removal of Ammonium to Enhance the Cultivation Performance of gdhA Derivative Pasteurella multocida B:2

2020 ◽  
Vol 8 (11) ◽  
pp. 1654
Author(s):  
Siti Nur Hazwani Oslan ◽  
Joo Shun Tan ◽  
Sahar Abbasiliasi ◽  
Ahmad Ziad Sulaiman ◽  
Mohd Zamri Saad ◽  
...  
Keyword(s):  
Growth Performance ◽  
Pasteurella Multocida ◽  
Culture Medium ◽  
Inhibitory Effect ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Internal Adsorption ◽  
Exchange Adsorption ◽  
Bioreactor System ◽  
Adsorption Resin

Growth of mutant gdhA Pasteurella multocida B:2 was inhibited by the accumulation of a by-product, namely ammonium in the culture medium during fermentation. The removal of this by-product during the cultivation of mutant gdhA P. multocida B:2 in a 2 L stirred-tank bioreactor integrated with an internal column using cation-exchange adsorption resin for the improvement of cell viability was studied. Different types of bioreactor system (dispersed and internal) with resins were successfully used for ammonium removal at different agitation speeds. The cultivation in a bioreactor integrated with an internal column demonstrated a significant improvement in growth performance of mutant gdhA P. multocida B:2 (1.05 × 1011 cfu/mL), which was 1.6-fold and 8.4-fold as compared to cultivation with dispersed resin (7.2 × 1010 cfu/mL) and cultivation without resin (1.25 × 1010 cfu/mL), respectively. The accumulation of ammonium in culture medium without resin (801 mg/L) was 1.24-fold and 1.37-fold higher than culture with dispersed resin (642.50 mg/L) and culture in the bioreactor integrated with internal adsorption (586.50 mg/L), respectively. Results from this study demonstrated that cultivation in a bioreactor integrated with the internal adsorption column in order to remove ammonium could reduce the inhibitory effect of this by-product and improve the growth performance of mutant gdhA P. multocida B:2.

Optimization of Continuously Stirred Tank Bioreactor Design for Cost Minimization: Effect of Microbial Species and Operating Conditions

2008 ◽  
Vol 6 (1) ◽  
Author(s):  
Erin E Powell ◽  
Gordon A Hill
Keyword(s):  
Culture Medium ◽  
Operating Cost ◽  
Oxygen Demand ◽  
Design Strategy ◽  
Aeration Rate ◽  
Operating Conditions ◽  
Stirred Tank ◽  
Optimum Operating ◽  
Stirred Tank Bioreactor ◽  
Microbial Species

The operation of continuously stirred tank bioreactors (CSTBs) at minimum cost is a major concern of operators. In this work, a CSTB design strategy is presented where impeller stirring speed and aeration rate are optimized to meet the oxygen demand of growing cells, simultaneously minimizing the capital and operating cost. The operating cost is limited to the cost of utilities. The optimization scheme assumes a given fermentor tank size, and that the properties of the culture medium and the oxygen respiratory requirements of the microorganisms being cultivated are known. It is possible to choose between two different turbine impellers during the design process. The equations, constraints, and the CSTB design strategy employed by the program are described. The effect of microbial species, ions in the culture medium, impeller style, as well as changing CSTB size and biomass input density on the optimum operating conditions, is examined. The mass transfer coefficient, gas holdup, mixing speed, and aeration rate are all reported at optimized cost conditions. A study of the effects of various parameters on the CSTB design are shown.

scholarly journals Performance of a continuously stirred tank bioreactor system connected in series for the biodegradation of thiocyanate and free cyanide

2017 ◽  
Vol 5 (2) ◽  
pp. 1936-1945 ◽  
Author(s):  
Lukhanyo Mekuto ◽  
Seteno K.O. Ntwampe ◽  
Clement E. Utomi ◽  
Mmabatho Mobo ◽  
John Baptist Mudumbi ◽  
...  
Keyword(s):  
Stirred Tank ◽  
Free Cyanide ◽  
Stirred Tank Bioreactor ◽  
In Series ◽  
Bioreactor System

Characterization of Hematopoietic Cell Expansion, Oxygen Uptake, and Glycolysis in a Controlled, Stirred-Tank Bioreactor System

1998 ◽  
Vol 14 (3) ◽  
pp. 466-472 ◽  
Author(s):  
P.C. Collins ◽  
L.K. Nielsen ◽  
S.D. Patel ◽  
E.T. Papoutsakis ◽  
W.M. Miller
Keyword(s):  
Oxygen Uptake ◽  
Cell Expansion ◽  
Hematopoietic Cell ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Bioreactor System

Stirred tank bioreactor culture combined with serum-/xenogeneic-free culture medium enables an efficient expansion of umbilical cord-derived mesenchymal stem/stromal cells

2016 ◽  
Vol 11 (8) ◽  
pp. 1048-1059 ◽  
Author(s):  
Amanda Mizukami ◽  
Ana Fernandes-Platzgummer ◽  
Joana G. Carmelo ◽  
Kamilla Swiech ◽  
Dimas T. Covas ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Stromal Cells ◽  
Culture Medium ◽  
Stirred Tank ◽  
Bioreactor Culture ◽  
Stirred Tank Bioreactor ◽  
Efficient Expansion

scholarly journals Residual Gas for Ethanol Production by Clostridium carboxidivorans in a Dual Impeller Stirred Tank Bioreactor (STBR)

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 199 ◽  
Author(s):  
Carolina Benevenuti ◽  
Marcelle Branco ◽  
Mariana do Nascimento-Correa ◽  
Alanna Botelho ◽  
Tatiana Ferreira ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Ethanol Production ◽  
Culture Medium ◽  
Biofuel Production ◽  
Stirred Tank ◽  
Low Flow ◽  
Syngas Fermentation ◽  
Stirred Tank Bioreactor ◽  
Industrial Gases ◽  
Clostridium Carboxidivorans

Recycling residual industrial gases and residual biomass as substrates to biofuel production by fermentation is an important alternative to reduce organic wastes and greenhouse gases emission. Clostridium carboxidivorans can metabolize gaseous substrates as CO and CO2 to produce ethanol and higher alcohols through the Wood-Ljungdahl pathway. However, the syngas fermentation is limited by low mass transfer rates. In this work, a syngas fermentation was carried out in serum glass bottles adding different concentrations of Tween® 80 in ATCC® 2713 culture medium to improve gas-liquid mass transfer. We observed a 200% increase in ethanol production by adding 0.15% (v/v) of the surfactant in the culture medium and a 15% increase in biomass production by adding 0.3% (v/v) of the surfactant in the culture medium. The process was reproduced in stirred tank bioreactor with continuous syngas low flow, and a maximum ethanol productivity of 0.050 g/L.h was achieved.

scholarly journals Characterization and performance of the Allegro™ STR 1000 single-use stirred tank bioreactor system

2015 ◽  
Vol 9 (S9) ◽  
Author(s):  
Kerem Irfan ◽  
Byron Rees ◽  
Tim Barrett ◽  
Bojan Markicevic ◽  
Camille Segarra
Keyword(s):  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Single Use ◽  
Bioreactor System ◽  
And Performance

scholarly journals Novel multi-strain probiotics reduces Pasteurella multocida induced fowl cholera mortality in broilers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rine Christopher Reuben ◽  
Shovon Lal Sarkar ◽  
Habiba Ibnat ◽  
Md. Ali Ahasan Setu ◽  
Pravas Chandra Roy ◽  
...  
Keyword(s):  
Growth Performance ◽  
Feed Efficiency ◽  
Biochemical Parameters ◽  
Pasteurella Multocida ◽  
White Blood Cells ◽  
Clinical Signs ◽  
Basal Diet ◽  
Prostaglandin E ◽  
Fowl Cholera ◽  
Anti Inflammatory

AbstractPasteurella multocida causes fowl cholera, a highly contagious poultry disease of global concern, causing significant ecological and economic challenges to the poultry industry each year. This study evaluated the effects of novel multi-strain probiotics consisting of Lactobacillus plantarum, L. fermentum, Pediococcus acidilactici, Enterococcus faecium and Saccharomyces cerevisiae on growth performance, intestinal microbiota, haemato-biochemical parameters and anti-inflammatory properties on broilers experimentally challenged with P. multocida. A total of 120 birds were fed with a basal diet supplemented with probiotics (108 CFU/kg) and then orally challenged with 108 CFU/mL of P. multocida. Probiotics supplementation significantly (P < 0.05) improved growth performance and feed efficiency as well as reducing (P < 0.05) the population of intestinal P. multocida, enterobacteria, and mortality. Haemato-biochemical parameters including total cholesterol, white blood cells (WBC), proteins, glucose, packed cell volume (PCV) and lymphocytes improved (P < 0.05) among probiotic fed birds when compared with the controls. Transcriptional profiles of anti-inflammatory genes including hypoxia inducible factor 1 alpha (HIF1A), tumor necrosis factor- (TNF) stimulated gene-6 (TSG-6) and prostaglandin E receptor 2 (PTGER2) in the intestinal mucosa were upregulated (P < 0.05) in probiotics fed birds. The dietary inclusion of the novel multi-strain probiotics improves growth performance, feed efficiency and intestinal health while attenuating inflammatory reaction, clinical signs and mortality associated with P. multocida infection in broilers.

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