Characterization of Hematopoietic Cell Expansion, Oxygen Uptake, and Glycolysis in a Controlled, Stirred-Tank Bioreactor System

1998 ◽  
Vol 14 (3) ◽  
pp. 466-472 ◽  
Author(s):  
P.C. Collins ◽  
L.K. Nielsen ◽  
S.D. Patel ◽  
E.T. Papoutsakis ◽  
W.M. Miller
Keyword(s):  
Oxygen Uptake ◽  
Cell Expansion ◽  
Hematopoietic Cell ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Bioreactor System

scholarly journals Performance of a continuously stirred tank bioreactor system connected in series for the biodegradation of thiocyanate and free cyanide

2017 ◽  
Vol 5 (2) ◽  
pp. 1936-1945 ◽  
Author(s):  
Lukhanyo Mekuto ◽  
Seteno K.O. Ntwampe ◽  
Clement E. Utomi ◽  
Mmabatho Mobo ◽  
John Baptist Mudumbi ◽  
...  
Keyword(s):  
Stirred Tank ◽  
Free Cyanide ◽  
Stirred Tank Bioreactor ◽  
In Series ◽  
Bioreactor System

scholarly journals Genomics Characterization of an Engineered Corynebacterium glutamicum in Bioreactor Cultivation Under Ionic Liquid Stress

2021 ◽  
Vol 9 ◽  
Author(s):  
Deepanwita Banerjee ◽  
Thomas Eng ◽  
Yusuke Sasaki ◽  
Aparajitha Srinivasan ◽  
Asun Oka ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Corynebacterium Glutamicum ◽  
Shake Flask ◽  
Differential Expression Analysis ◽  
Distinct Pattern ◽  
Stirred Tank ◽  
Bioreactor Cultivation ◽  
Stirred Tank Bioreactor ◽  
Scale Transition

Corynebacterium glutamicum is an ideal microbial chassis for production of valuable bioproducts including amino acids and next generation biofuels. Here we resequence engineered isopentenol (IP) producing C. glutamicum BRC-JBEI 1.1.2 strain and assess differential transcriptional profiles using RNA sequencing under industrially relevant conditions including scale transition and compare the presence vs absence of an ionic liquid, cholinium lysinate ([Ch][Lys]). Analysis of the scale transition from shake flask to bioreactor with transcriptomics identified a distinct pattern of metabolic and regulatory responses needed for growth in this industrial format. These differential changes in gene expression corroborate altered accumulation of organic acids and bioproducts, including succinate, acetate, and acetoin that occur when cells are grown in the presence of 50 mM [Ch][Lys] in the stirred-tank reactor. This new genome assembly and differential expression analysis of cells grown in a stirred tank bioreactor clarify the cell response of an C. glutamicum strain engineered to produce IP.

Computations of Flow Environments in Medium-Scale Stirred-Tank Bioreactors for Stem Cell Expansion

2014 ◽  
Author(s):  
Petar Liovic ◽  
Ilija D. Šutalo ◽  
Laurence Meagher ◽  
George O. Lovrecz
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Cell Expansion ◽  
Model Development ◽  
Experimental Studies ◽  
Spatiotemporal Variability ◽  
Stirred Tank ◽  
Shear Stresses ◽  
Stirred Tank Bioreactor ◽  
Stem Cell Expansion

The flow in a New Brunswick Scientific (NBS (now Eppendorf)) 5 L stirred-tank bioreactor (STR) partially filled with 2.2 L of water and agitated at 60 rpm using a pitched-blade impeller is studied in this work, to determine the suitability of the configuration for expanding stem cell lines. Computational Fluid Dynamics (CFD) model development and testing in this work has found Large Eddy Simulation (LES) to be essential for model fidelity and for capturing spatiotemporal stress fluctuations. Stresses were at levels similar to or even higher than those known to damage stem cells or modulate their cellular function to favour differentiation instead of phenotype maintenance. The results raise questions as to the appropriateness of such STRs for stem cell expansion, and motivate better experimental studies to properly quantify the spatiotemporal variability in fluid shear stresses and its effect on stem cell expansion and stem cell fate.

scholarly journals Genomics Characterization of an engineered Corynebacterium glutamicum in Bioreactor Cultivation under Ionic Liquid Stress

2021 ◽  
Author(s):  
Deepanwita Banerjee ◽  
Thomas Eng ◽  
Yusuke Sasaki ◽  
Aparajitha Srinivasan ◽  
Asun Oka ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Corynebacterium Glutamicum ◽  
Shake Flask ◽  
Differential Expression Analysis ◽  
Distinct Pattern ◽  
Stirred Tank ◽  
Bioreactor Cultivation ◽  
Stirred Tank Bioreactor ◽  
Scale Transition

AbstractCorynebacterium glutamicum is an ideal microbial chassis for the production of valuable bioproducts including amino acids and next-generation biofuels. Here we resequence engineered isopentenol (IP) producing C. glutamicum BRC-JBEI 1.1.2 strain and assess differential transcriptional profiles using RNA sequencing under industrially relevant conditions including scale transition and compare the presence vs. absence of an ionic liquid, cholinium lysinate ([Ch][Lys]). Analysis of the scale transition from shake flask to bioreactor with transcriptomics identified a distinct pattern of metabolic and regulatory responses needed for growth in this industrial format. These differential changes in gene expression corroborate altered accumulation of organic acids and bioproducts, including succinate, acetate, and acetoin that occur when cells are grown in the presence of 50mM [Ch][Lys] in the stirred-tank reactor. This new genome assembly and differential expression analysis of cells grown in a stirred tank bioreactor clarify the cell response of a C. glutamicum strain engineered to produce IP.

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