Stirred tank bioreactor culture combined with serum-/xenogeneic-free culture medium enables an efficient expansion of umbilical cord-derived mesenchymal stem/stromal cells

2016 ◽  
Vol 11 (8) ◽  
pp. 1048-1059 ◽  
Author(s):  
Amanda Mizukami ◽  
Ana Fernandes-Platzgummer ◽  
Joana G. Carmelo ◽  
Kamilla Swiech ◽  
Dimas T. Covas ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Stromal Cells ◽  
Culture Medium ◽  
Stirred Tank ◽  
Bioreactor Culture ◽  
Stirred Tank Bioreactor ◽  
Efficient Expansion

Hypoxia priming improves in vitro angiogenic properties of umbilical cord derived-mesenchymal stromal cells expanded in stirred-tank bioreactor

2021 ◽  
Vol 168 ◽  
pp. 107949
Author(s):  
Nádia C. Noronha ◽  
Amanda Mizukami ◽  
Maristela D. Orellana ◽  
Maria C. Oliveira ◽  
Dimas T. Covas ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor

High yield of human umbilical cord-derived mesenchymal stromal cells in an animal component-free culture medium

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S111-S112
Author(s):  
R. Wagey ◽  
K. Bertram ◽  
M. Elliott ◽  
A. Eaves ◽  
S. Szilvassy ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Culture Medium ◽  
High Yield ◽  
Human Umbilical Cord ◽  
Animal Component

Optimization of Continuously Stirred Tank Bioreactor Design for Cost Minimization: Effect of Microbial Species and Operating Conditions

2008 ◽  
Vol 6 (1) ◽  
Author(s):  
Erin E Powell ◽  
Gordon A Hill
Keyword(s):  
Culture Medium ◽  
Operating Cost ◽  
Oxygen Demand ◽  
Design Strategy ◽  
Aeration Rate ◽  
Operating Conditions ◽  
Stirred Tank ◽  
Optimum Operating ◽  
Stirred Tank Bioreactor ◽  
Microbial Species

The operation of continuously stirred tank bioreactors (CSTBs) at minimum cost is a major concern of operators. In this work, a CSTB design strategy is presented where impeller stirring speed and aeration rate are optimized to meet the oxygen demand of growing cells, simultaneously minimizing the capital and operating cost. The operating cost is limited to the cost of utilities. The optimization scheme assumes a given fermentor tank size, and that the properties of the culture medium and the oxygen respiratory requirements of the microorganisms being cultivated are known. It is possible to choose between two different turbine impellers during the design process. The equations, constraints, and the CSTB design strategy employed by the program are described. The effect of microbial species, ions in the culture medium, impeller style, as well as changing CSTB size and biomass input density on the optimum operating conditions, is examined. The mass transfer coefficient, gas holdup, mixing speed, and aeration rate are all reported at optimized cost conditions. A study of the effects of various parameters on the CSTB design are shown.

scholarly journals Process development for expansion of human mesenchymal stromal cells in a 50L single-use stirred tank bioreactor

2017 ◽  
Vol 120 ◽  
pp. 49-62 ◽  
Author(s):  
Tristan Lawson ◽  
Daniel E. Kehoe ◽  
Aletta C. Schnitzler ◽  
Peter J. Rapiejko ◽  
Kara A. Der ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Process Development ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Human Mesenchymal Stromal Cells ◽  
Single Use

Efficient expansion of mesenchymal stromal cells from umbilical cord under low serum conditions

Cytotherapy ◽  
2009 ◽  
pp. 1-11 ◽  
Author(s):  
John Girdlestone ◽  
Vasanti Limbani ◽  
Antony Cutler ◽  
Cristina Navarrete
Keyword(s):  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Efficient Expansion ◽  
Low Serum

scholarly journals Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Florian Petry ◽  
J. Robert Smith ◽  
Jasmin Leber ◽  
Denise Salzig ◽  
Peter Czermak ◽  
...  
Keyword(s):  
Dynamic System ◽  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Growth Surface ◽  
Human Umbilical Cord ◽  
Expansion Process ◽  
Stirred Tank Bioreactor ◽  
Large Expansion ◽  
Process Transfer

The great properties of human mesenchymal stromal cells (hMSCs) make these cells an important tool in regenerative medicine. Because of the limitations of hMSCs derived from the bone marrow during isolation and expansion, hMSCs derived from the umbilical cord stroma are a great alternative to overcome these issues. For a large expansion of these cells, we performed a process transfer from static culture to a dynamic system. For this reason, a microcarrier selection out of five microcarrier types was made to achieve a suitable growth surface for the cells. The growth characteristics and metabolite consumption and production were used to compare the cells growth in 12-well plate and spinner flask. The goal to determine relevant process parameters to transfer the expansion process into a stirred tank bioreactor was achieved.

scholarly journals Residual Gas for Ethanol Production by Clostridium carboxidivorans in a Dual Impeller Stirred Tank Bioreactor (STBR)

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 199 ◽  
Author(s):  
Carolina Benevenuti ◽  
Marcelle Branco ◽  
Mariana do Nascimento-Correa ◽  
Alanna Botelho ◽  
Tatiana Ferreira ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Ethanol Production ◽  
Culture Medium ◽  
Biofuel Production ◽  
Stirred Tank ◽  
Low Flow ◽  
Syngas Fermentation ◽  
Stirred Tank Bioreactor ◽  
Industrial Gases ◽  
Clostridium Carboxidivorans

Recycling residual industrial gases and residual biomass as substrates to biofuel production by fermentation is an important alternative to reduce organic wastes and greenhouse gases emission. Clostridium carboxidivorans can metabolize gaseous substrates as CO and CO2 to produce ethanol and higher alcohols through the Wood-Ljungdahl pathway. However, the syngas fermentation is limited by low mass transfer rates. In this work, a syngas fermentation was carried out in serum glass bottles adding different concentrations of Tween® 80 in ATCC® 2713 culture medium to improve gas-liquid mass transfer. We observed a 200% increase in ethanol production by adding 0.15% (v/v) of the surfactant in the culture medium and a 15% increase in biomass production by adding 0.3% (v/v) of the surfactant in the culture medium. The process was reproduced in stirred tank bioreactor with continuous syngas low flow, and a maximum ethanol productivity of 0.050 g/L.h was achieved.

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