scholarly journals Characterization of Scardovia wiggsiae Biofilm by Original Scanning Electron Microscopy Protocol

2020 ◽  
Vol 8 (6) ◽  
pp. 807 ◽  
Author(s):  
Maurizio Bossù ◽  
Laura Selan ◽  
Marco Artini ◽  
Michela Relucenti ◽  
Giuseppe Familiari ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bacterial Species ◽  
High Vacuum ◽  
Innovative Strategies ◽  
Ultrastructural Characterization ◽  
Severe Manifestation ◽  
Original Procedure ◽  
Scanning Electron

Early childhood caries (ECC) is a severe manifestation of carious pathology with rapid and disruptive progression. The ECC microbiota includes a wide variety of bacterial species, among which is an anaerobic newly named species, Scardovia wiggsiae, a previously unidentified Bifidobacterium. Our aim was to provide the first ultrastructural characterization of S. wiggsiae and its biofilm by scanning electron microscopy (SEM) using a protocol that faithfully preserved the biofilm architecture and allowed an investigation at very high magnifications (order of nanometers) and with the appropriate resolution. To accomplish this task, we analyzed Streptococcus mutans’ biofilm by conventional SEM and VP-SEM protocols, in addition, we developed an original procedure, named OsO4-RR-TA-IL, which avoids dehydration, drying and sputter coating. This innovative protocol allowed high-resolution and high-magnification imaging (from 10000× to 35000×) in high-vacuum and high-voltage conditions. After comparing three methods, we chose OsO4-RR-TA-IL to investigate S. wiggsiae. It appeared as a fusiform elongated bacterium, without surface specialization, arranged in clusters and submerged in a rich biofilm matrix, which showed a well-developed micro-canalicular system. Our results provide the basis for the development of innovative strategies to quantify the effects of different treatments, in order to establish the best option to counteract ECC in pediatric patients.

Ultrastructural characterization of cytomegalovirus infected and interferon producing human foreskin cells

1990 ◽  
Vol 48 (3) ◽  
pp. 312-313
Author(s):  
J. Rodriquez ◽  
T.O. Moninger ◽  
D. Walker ◽  
K. C. Moore
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Cytomegalovirus ◽  
Beta Interferon ◽  
Ultrastructural Characterization ◽  
Scanning Electron

Published work indicates that human cytomegalovirus (CMV) is sensitive to the action of beta interferon (HuIFNb) and can induce the production of INF in cultures of human foreskin (HF) cells. These findings are consistent with a role for endogenous IFN in the establishment and maintenance of persistent CMV infections. Moreover HF cells can be primed with IFN and these primed cells can be induced to produce large concentrations of IFN after exposure to CMV. These findings support the hypothesis formulated to explain the larger-than-expected concentrations of IFN detected in cultures exposed to low MOIs (<0.01), namely, that the phenomenon observed involved endogenous priming, that is production of IFN by the small percentage of the cells infected by the inoculum virus. This results in priming of the remaining cells by IFN, and finally induction of the primed cells by progeny CMV released from the cells infected originally.To lend support to this hypothesis we will follow the attachment, penetration, and release stages of the CMV replication by transmission and scanning electron microscopy.

scholarly journals Ultrastructural characterization of cystic fibrosis sputum using atomic force and scanning electron microscopy

2012 ◽  
Vol 11 (2) ◽  
pp. 84-92 ◽  
Author(s):  
Reinhard Manzenreiter ◽  
Ferry Kienberger ◽  
Veronica Marcos ◽  
Kurt Schilcher ◽  
Wolf D. Krautgartner ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cystic Fibrosis ◽  
Scanning Electron Microscopy ◽  
Cystic Fibrosis Sputum ◽  
Atomic Force ◽  
Ultrastructural Characterization ◽  
Scanning Electron

Realization and Characterization of Graphene on 4H-SiC for Tera-Hertz Transistors

2015 ◽  
Vol 821-823 ◽  
pp. 941-944 ◽  
Author(s):  
Julien Pezard ◽  
Jeremy Lhuillier ◽  
Zakarya El-Friach ◽  
Véronique Soulière ◽  
Bertrand Vilquin ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Field Effect ◽  
Field Effect Transistors ◽  
High Vacuum ◽  
Ultra High Vacuum ◽  
Graphene Layers ◽  
Electrical Characterizations ◽  
Scanning Electron

Few layers graphene has been grown on 4H-SiC. Since this material has outstanding electronic properties, we aimed fabricating graphene field-effect transistors on silicon carbide wafer. Growth of the graphene layers was made by e-beam sublimation of silicium under ultra high vacuum (UHV). These layers were patterned and used as channels of transistors with source and drain made of P+ SiC. The different technological steps were checked through Raman spectroscopy, Scanning Electron Microscopy (SEM), and electrical characterizations.

Isolasi Dan Karakterisasi Bentonit Alam Menjadi Nanopartikel Monmorillonit

2018 ◽  
Vol 3 (1) ◽  
pp. 12 ◽  
Author(s):  
Zaimahwati Zaimahwati ◽  
Yuniati Yuniati ◽  
Ramzi Jalal ◽  
Syahman Zhafiri ◽  
Yuli Yetri
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Particle Size ◽  
Surface Morphology ◽  
Average Diameter ◽  
Particle Size Analyzer ◽  
Ft Ir ◽  
Scanning Electron ◽  
Sedimentation Processes

<p>Pada penelitian ini telah dilakukan isolasi dan karakterisasi bentonit alam menjadi nanopartikel montmorillonit. Bentonit alam yang digunakan diambil dari desa Blangdalam, Kecamatan Nisam Kabupaten Aceh Utara.  Proses isolasi meliputi proses pelarutan dengan aquades, ultrasonic dan proses sedimentasi. Untuk mengetahui karakterisasi montmorillonit dilakukan uji FT-IR, X-RD dan uji morfologi permukaan dengan Scanning Electron Microscopy (SEM). Partikel size analyzer untuk menganalisis dan menentukan ukuran nanopartikel dari isolasi bentonit alam. Dari hasil penelitian didapat ukuran nanopartikel montmorillonit hasil isolasi dari bentonit alam diperoleh berdiameter rata-rata 82,15 nm.</p><p><em>In this research we have isolated and characterized natural bentonite into montmorillonite nanoparticles. Natural bentonite used was taken from Blangdalam village, Nisam sub-district, North Aceh district. The isolation process includes dissolving process with aquades, ultrasonic and sedimentation processes.  The characterization of montmorillonite, FT-IR, X-RD and surface morphology test by Scanning Electron Microscopy (SEM). Particle size analyzer to analyze and determine the size of nanoparticles from natural bentonite insulation. From the research results obtained the size of montmorillonite nanoparticles isolated from natural bentonite obtained an average diameter of 82.15 nm.</em></p>

scholarly journals Design and Characterization of Microscale Auxetic and Anisotropic Structures Fabricated by Multiphoton Lithography

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 446
Author(s):  
Ioannis Spanos ◽  
Zacharias Vangelatos ◽  
Costas Grigoropoulos ◽  
Maria Farsari
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Mechanical Performance ◽  
Element Analysis ◽  
Mechanical Responses ◽  
Multiphoton Lithography ◽  
Anisotropic Structures ◽  
Scanning Electron

The need for control of the elastic properties of architected materials has been accentuated due to the advances in modelling and characterization. Among the plethora of unconventional mechanical responses, controlled anisotropy and auxeticity have been promulgated as a new avenue in bioengineering applications. This paper aims to delineate the mechanical performance of characteristic auxetic and anisotropic designs fabricated by multiphoton lithography. Through finite element analysis the distinct responses of representative topologies are conveyed. In addition, nanoindentation experiments observed in-situ through scanning electron microscopy enable the validation of the modeling and the observation of the anisotropic or auxetic phenomena. Our results herald how these categories of architected materials can be investigated at the microscale.

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