scholarly journals Ultrastructural characterization of cystic fibrosis sputum using atomic force and scanning electron microscopy

2012 ◽  
Vol 11 (2) ◽  
pp. 84-92 ◽  
Author(s):  
Reinhard Manzenreiter ◽  
Ferry Kienberger ◽  
Veronica Marcos ◽  
Kurt Schilcher ◽  
Wolf D. Krautgartner ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cystic Fibrosis ◽  
Scanning Electron Microscopy ◽  
Cystic Fibrosis Sputum ◽  
Atomic Force ◽  
Ultrastructural Characterization ◽  
Scanning Electron

Characterization of Multiple Functional Solid Line of Graphite Ink Surface Printed by Micro-Flexographic

2015 ◽  
Vol 752-753 ◽  
pp. 1379-1383
Author(s):  
M.I. Maksud ◽  
Mohd Sallehuddin Yusof ◽  
Zaidi Embong
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Photoelectron Spectroscopy ◽  
Printing Plate ◽  
X Ray ◽  
Force Microscopy ◽  
Atomic Force ◽  
Flexographic Printing ◽  
Scanning Electron

The purpose of this paper is to study a ink surface morphology, quantify the chemical composition involved in processing of graphite ink printed by flexographic printing. The methodology is to use surface sensitive technique, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) and Field Emission Scanning Electron Microscopy (FESEM). As a finding we successfully achieved 25 micron lines array using PDMS printing plate. The Originality and value of this work is surface sensitive techniques like XPS, AFM and FESEM were exclusively used in order to characterize graphite inks printed by flexographic method, using PDMS printing plate.

scholarly journals Characterization of Scardovia wiggsiae Biofilm by Original Scanning Electron Microscopy Protocol

2020 ◽  
Vol 8 (6) ◽  
pp. 807 ◽  
Author(s):  
Maurizio Bossù ◽  
Laura Selan ◽  
Marco Artini ◽  
Michela Relucenti ◽  
Giuseppe Familiari ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bacterial Species ◽  
High Vacuum ◽  
Innovative Strategies ◽  
Ultrastructural Characterization ◽  
Severe Manifestation ◽  
Original Procedure ◽  
Scanning Electron

Early childhood caries (ECC) is a severe manifestation of carious pathology with rapid and disruptive progression. The ECC microbiota includes a wide variety of bacterial species, among which is an anaerobic newly named species, Scardovia wiggsiae, a previously unidentified Bifidobacterium. Our aim was to provide the first ultrastructural characterization of S. wiggsiae and its biofilm by scanning electron microscopy (SEM) using a protocol that faithfully preserved the biofilm architecture and allowed an investigation at very high magnifications (order of nanometers) and with the appropriate resolution. To accomplish this task, we analyzed Streptococcus mutans’ biofilm by conventional SEM and VP-SEM protocols, in addition, we developed an original procedure, named OsO4-RR-TA-IL, which avoids dehydration, drying and sputter coating. This innovative protocol allowed high-resolution and high-magnification imaging (from 10000× to 35000×) in high-vacuum and high-voltage conditions. After comparing three methods, we chose OsO4-RR-TA-IL to investigate S. wiggsiae. It appeared as a fusiform elongated bacterium, without surface specialization, arranged in clusters and submerged in a rich biofilm matrix, which showed a well-developed micro-canalicular system. Our results provide the basis for the development of innovative strategies to quantify the effects of different treatments, in order to establish the best option to counteract ECC in pediatric patients.

Ultrastructural characterization of cytomegalovirus infected and interferon producing human foreskin cells

1990 ◽  
Vol 48 (3) ◽  
pp. 312-313
Author(s):  
J. Rodriquez ◽  
T.O. Moninger ◽  
D. Walker ◽  
K. C. Moore
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Cytomegalovirus ◽  
Beta Interferon ◽  
Ultrastructural Characterization ◽  
Scanning Electron

Published work indicates that human cytomegalovirus (CMV) is sensitive to the action of beta interferon (HuIFNb) and can induce the production of INF in cultures of human foreskin (HF) cells. These findings are consistent with a role for endogenous IFN in the establishment and maintenance of persistent CMV infections. Moreover HF cells can be primed with IFN and these primed cells can be induced to produce large concentrations of IFN after exposure to CMV. These findings support the hypothesis formulated to explain the larger-than-expected concentrations of IFN detected in cultures exposed to low MOIs (<0.01), namely, that the phenomenon observed involved endogenous priming, that is production of IFN by the small percentage of the cells infected by the inoculum virus. This results in priming of the remaining cells by IFN, and finally induction of the primed cells by progeny CMV released from the cells infected originally.To lend support to this hypothesis we will follow the attachment, penetration, and release stages of the CMV replication by transmission and scanning electron microscopy.

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