Ultrastructural characterization of the intestine of the Eurasian common moorhen using scanning electron microscopy and light microscopy

2021 ◽  
Author(s):  
Basma G. Hanafy ◽  
Mohamed M. A. Abumandour ◽  
Ramadan Kandyle ◽  
Naglaa F. Bassuoni
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Ultrastructural Characterization ◽  
Scanning Electron

scholarly journals Characterization of Scardovia wiggsiae Biofilm by Original Scanning Electron Microscopy Protocol

2020 ◽  
Vol 8 (6) ◽  
pp. 807 ◽  
Author(s):  
Maurizio Bossù ◽  
Laura Selan ◽  
Marco Artini ◽  
Michela Relucenti ◽  
Giuseppe Familiari ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bacterial Species ◽  
High Vacuum ◽  
Innovative Strategies ◽  
Ultrastructural Characterization ◽  
Severe Manifestation ◽  
Original Procedure ◽  
Scanning Electron

Early childhood caries (ECC) is a severe manifestation of carious pathology with rapid and disruptive progression. The ECC microbiota includes a wide variety of bacterial species, among which is an anaerobic newly named species, Scardovia wiggsiae, a previously unidentified Bifidobacterium. Our aim was to provide the first ultrastructural characterization of S. wiggsiae and its biofilm by scanning electron microscopy (SEM) using a protocol that faithfully preserved the biofilm architecture and allowed an investigation at very high magnifications (order of nanometers) and with the appropriate resolution. To accomplish this task, we analyzed Streptococcus mutans’ biofilm by conventional SEM and VP-SEM protocols, in addition, we developed an original procedure, named OsO4-RR-TA-IL, which avoids dehydration, drying and sputter coating. This innovative protocol allowed high-resolution and high-magnification imaging (from 10000× to 35000×) in high-vacuum and high-voltage conditions. After comparing three methods, we chose OsO4-RR-TA-IL to investigate S. wiggsiae. It appeared as a fusiform elongated bacterium, without surface specialization, arranged in clusters and submerged in a rich biofilm matrix, which showed a well-developed micro-canalicular system. Our results provide the basis for the development of innovative strategies to quantify the effects of different treatments, in order to establish the best option to counteract ECC in pediatric patients.

Ultrastructural characterization of cytomegalovirus infected and interferon producing human foreskin cells

1990 ◽  
Vol 48 (3) ◽  
pp. 312-313
Author(s):  
J. Rodriquez ◽  
T.O. Moninger ◽  
D. Walker ◽  
K. C. Moore
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Cytomegalovirus ◽  
Beta Interferon ◽  
Ultrastructural Characterization ◽  
Scanning Electron

Published work indicates that human cytomegalovirus (CMV) is sensitive to the action of beta interferon (HuIFNb) and can induce the production of INF in cultures of human foreskin (HF) cells. These findings are consistent with a role for endogenous IFN in the establishment and maintenance of persistent CMV infections. Moreover HF cells can be primed with IFN and these primed cells can be induced to produce large concentrations of IFN after exposure to CMV. These findings support the hypothesis formulated to explain the larger-than-expected concentrations of IFN detected in cultures exposed to low MOIs (<0.01), namely, that the phenomenon observed involved endogenous priming, that is production of IFN by the small percentage of the cells infected by the inoculum virus. This results in priming of the remaining cells by IFN, and finally induction of the primed cells by progeny CMV released from the cells infected originally.To lend support to this hypothesis we will follow the attachment, penetration, and release stages of the CMV replication by transmission and scanning electron microscopy.

Characterization of a peg-like terminal NOR structure with light microscopy and high-resolution scanning electron microscopy

Chromosoma ◽  
2005 ◽  
Vol 115 (1) ◽  
pp. 50-59 ◽  
Author(s):  
Elizabeth Schroeder-Reiter ◽  
Andreas Houben ◽  
Jürke Grau ◽  
Gerhard Wanner
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Light Microscopy ◽  
Scanning Electron

scholarly journals Characterization of Iberian species of the genus Pungentus Thorne & Swanger, 1936 (Nematoda, Dorylaimida, Nordiidae)

2013 ◽  
Author(s):  
Reyes Peña-Santiago ◽  
Marcel Ciobanu ◽  
Joaquin Abolafia
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Iberian Peninsula ◽  
Line Drawings ◽  
In The Wild ◽  
Scanning Electron ◽  
Cultivated Soils

Several populations of four known species of the genus Pungentus (P. clavatus, P. engadinensis, P. marietani and P. silvestris), collected in the wild and in cultivated soils from the Iberian Peninsula, are studied. Detailed redescriptions and morphometrics are presented for each species. Illustrations are provided, including line drawings, light microscopy pictures of the four species as well as scanning electron microscopy observations of P. engadinensis. The Iberian populations are compared to type and other known populations, and new data are given that provide a better characterization of these taxa. Pungentus engadinensis is the most widely distributed species in the Iberian Peninsula.

Palynological characterization of species of Verbenaceae J. St.-Hil. and Lamiaceae Martinov (Lamiales Bromhead)

2017 ◽  
Vol 4 (2) ◽  
Author(s):  
Gabriele Patricia Vitoria da Silva ◽  
Bruna Tereza Possamai ◽  
Gabriel da Rosa Schroeder ◽  
Nilton Paulo Vieira Junior ◽  
Enderlei Dec ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Pollen Grains ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
Scanning Electron

Clerodendrum splendens A. Chev., Clerodendrum x speciosum Tiej. & Binn, Clerodendrum thomsonae Balf. F., Clerodendrum ugandense L., Congea tomentosa Roxb., Duranta erecta L., Petrea volubilis L. and Petrea volubilis f. albiflora (Standl.) Standl. pollen grains were acetolyzed, photographed and measured under light microscopy and scanning electron microscopy. Values presented are averages in micrometers. Grains are monads, radially symmetrical, isopolar, large (C. ugandense, very large, C. tomentosa, small-medium and D. erecta, medium),tricolpate (P. volubilis f. albiflora, dimorphic grains with 3-4 colpus). Ambitus is circular (C. tomentosa and D. erecta, sub-circular, P. volubilis , triangular, P. volubilis f. albiflora, triangular-quadrangular). The form is oblate-spheroidal (C. splendens, C. x speciosum, C. ugandense), prolate-spheroidal (C. thomsonae), prolate (C. tomentosa), suboblate (D. erecta) and oblate (P. volubilis, P. volubilis f. albiflora). Exine thickness is in C. splendens 4,28, C. x speciosum 4,19, C. ugandense 4,33, C. thomsonae 4,18, C. tomentosa 1,4, D. erecta 1,55, P. volubilis 2,49, P. volubilis f. albiflora 2,68. Ornamentation is micro-echinate (C. splendens, C. x speciosum, C. thomsonae), echinate (C. ugandense), reticulate (C. tomentosa), psilate (D. erecta, P. volubilis, P. volubulis f. albiflora). Duranta and Petrea are close to Verbenaceae pattern, Congea to Lamiaceae and Clerodendrum loosely to Lamiaceae.

The characterization of rapidly solidified Al-8Fe-2Mo powder

1986 ◽  
Vol 44 ◽  
pp. 546-547
Author(s):  
H.K. Plummer ◽  
W.T. Donlon ◽  
J.E. Allison ◽  
S. Shinozaki
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Analytical Electron Microscopy ◽  
Powder Sample ◽  
Two Component ◽  
Analytical Electron ◽  
Rapidly Solidified ◽  
Scanning Electron

A centrifugally atomized rapidly solidified Al-8Fe-2Mo powder was characterized by Light Microscopy (LM), Scanning Electron Microscopy (SEM) and Analytical Electron Microscopy (AEM). The powder sample was studied in an as-solidified state and after annealing in 1 at. argon for 6 hr at 400°C. Further studies have characterized the consolidated bars produced by compaction and extrusion of these powders.The 30 to 150 μm powders are spherical as seen in the SEM (Fig.1) with small (5 to 15 μm) satellite powders sometimes attached in external (A,B Fig.1) and internal (A Fig.2C) locations. A comparison of powders (epoxy mounted and polished) by both the LM (Fig. 2A) and the SEM (Figs. 2B and 2C) reveals a two component microstructure with occasional voids.

scholarly journals Ultrastructural characterization of cystic fibrosis sputum using atomic force and scanning electron microscopy

2012 ◽  
Vol 11 (2) ◽  
pp. 84-92 ◽  
Author(s):  
Reinhard Manzenreiter ◽  
Ferry Kienberger ◽  
Veronica Marcos ◽  
Kurt Schilcher ◽  
Wolf D. Krautgartner ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cystic Fibrosis ◽  
Scanning Electron Microscopy ◽  
Cystic Fibrosis Sputum ◽  
Atomic Force ◽  
Ultrastructural Characterization ◽  
Scanning Electron
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