scholarly journals A Metabolomic-Based Evaluation of the Role of Commensal Microbiota throughout the Gastrointestinal Tract in Mice

2018 ◽  
Vol 6 (4) ◽  
pp. 101 ◽  
Author(s):  
Yuri Yamamoto ◽  
Yumiko Nakanishi ◽  
Shinnosuke Murakami ◽  
Wanping Aw ◽  
Tomoya Tsukimi ◽  
...  
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Large Intestine ◽  
Rrna Gene ◽  
Commensal Microbiota ◽  
Flight Mass Spectrometry ◽  
Specific Pathogen ◽  
Gastrointestinal Microbes ◽  
Small And Large Intestine

Commensal microbiota colonize the surface of our bodies. The inside of the gastrointestinal tract is one such surface that provides a habitat for them. The gastrointestinal tract is a long organ system comprising of various parts, and each part possesses various functions. It has been reported that the composition of intestinal luminal metabolites between the small and large intestine are different; however, comprehensive metabolomic and commensal microbiota profiles specific to each part of the gastrointestinal lumen remain obscure. In this study, by using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS)-based metabolome and 16S rRNA gene-based microbiome analyses of specific pathogen-free (SPF) and germ-free (GF) murine gastrointestinal luminal profiles, we observed the different roles of commensal microbiota in each part of the gastrointestinal tract involved in carbohydrate metabolism and nutrient production. We found that the concentrations of most amino acids in the SPF small intestine were higher than those in the GF small intestine. Furthermore, sugar alcohols such as mannitol and sorbitol accumulated only in the GF large intestine, but not in the SPF large intestine. On the other hand, pentoses, such as arabinose and xylose, gradually accumulated from the cecum to the colon only in SPF mice, but were undetected in GF mice. Correlation network analysis between the gastrointestinal microbes and metabolites showed that niacin metabolism might be correlated to Methylobacteriaceae. Collectively, commensal microbiota partially affects the gastrointestinal luminal metabolite composition based on their metabolic dynamics, in cooperation with host digestion and absorption.

Pyrosequencing-based analysis of the complex microbiota located in the gastrointestinal tracts of growing-finishing pigs

2019 ◽  
Vol 59 (5) ◽  
pp. 870 ◽  
Author(s):  
J. Wang ◽  
Y. Han ◽  
J. Z. Zhao ◽  
Z. J. Zhou ◽  
H. Fan
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Large Intestine ◽  
Production Efficiency ◽  
Rrna Gene ◽  
Finishing Pigs ◽  
Gastrointestinal Microbiota ◽  
Bacterial Phyla ◽  
Principal Coordinates ◽  
Colon And Rectum

The commensal gut microbial communities play an important role in the health and production efficiency of growing-finishing pigs. This study aimed to analyse the composition and diversity of the microbiota in the gastrointestinal tract sections (stomach, duodenum, jejunum, ileum, caecum, colon and rectum) of growing-finishing pigs. This analysis was assessed using 454 pyrosequencing targeting the V3–V6 region of the 16S rRNA gene. Samples were collected from 20, healthy pigs aged 24 weeks and weighing 115.9 ± 5.4 kg. The dominant bacterial phyla in the various gastrointestinal tract sections were Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria. At the genus level, Prevotella, unclassified Lachnospiraceae, Ruminococcus, unclassified Ruminococcaceae and Oscillospira were more abundant in the large intestine than in the stomach and the small intestine. Unclassified Peptostreptococcaceae and Corynebacterium were more abundant in the small intestine than in the stomach and the large intestine. Shuttleworthia, unclassified Veillonellaceae and Mitsuokella were more abundant in the stomach than in the small and large intestines. At the species level, M. el.s.d.enii and M. multacida were predominant in the stomach. In addition, P. stercorea, P. copri, C. butyricum, R. flavefaciens and R. bromii were significantly more abundant in the large intestine than in the stomach and the small intestine. B. pseudolongum and B. thermacidophilum were significantly more abundant in the small intestine than in the stomach and the large intestine. Principal coordinates analysis showed that the overall composition of the pig gastrointestinal microbiota could be clustered into three groups: stomach, small intestine (duodenum, jejunum and ileum) and large intestine (caecum, colon and rectum). Venn diagrams illustrated the distribution of shared and specific operational taxonomic units among the various gastrointestinal tract sections.

scholarly journals Electromagnetic Fields Modify Redox Balance in the Rat Gastrointestinal Tract

2021 ◽  
Vol 9 ◽  
Author(s):  
Karolina Sieroń ◽  
Katarzyna Knapik ◽  
Grzegorz Onik ◽  
Ewa Romuk ◽  
Ewa Birkner ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Salivary Glands ◽  
Electromagnetic Fields ◽  
Large Intestine ◽  
Mobile Phones ◽  
Low Frequency ◽  
Control Group ◽  
Small And Large Intestine

Objective: The aim of the study was to assess the influence of electromagnetic fields with divergent physical properties on the prooxidative and antioxidative balances in homogenates of the tongue, salivary glands, esophagus, stomach, and small and large intestines of rats.Material and Methods: Forty rats were randomly divided into four equal groups, namely, a control group, a group exposed to low-frequency electromagnetic fields (LF-EMFs; frequency: 50 Hz; intensity: 10 kV/m; magnetic induction: 4.3 pT), a group exposed to radiofrequency electromagnetic fields (RF-EMFs) emitted by mobile phones (frequency: 900 MHz), and a group exposed simultaneously to LF-EMFs and RF-EMFs emitted by mobile phones. After 28 consecutive days of the experiment, the following pro- and antioxidative markers were assessed in the gastrointestinal tract homogenates: superoxide dismutase (SOD) and its two isoenzymes (Mn-SOD, Cu,Zn-SOD) catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), total antioxidative capacity (TAC), total oxidative status (TOS), and malondialdehyde (MDA).Results: In rats exposed to LF-EMFs, higher concentrations of the markers of prooxidant processes, MDA or TOS, were observed in the salivary glands, esophagus, and small intestine homogenates in comparison with the control group. Additionally, in the group of rats opposite to the control, antioxidant activity was observed. The main differences included a higher activity of Cu,Zn-SOD in homogenates of the tongue, salivary glands, and esophagus as well as decreased activity of CAT in homogenates of the tongue, esophagus, and small intestine. In animals exposed to RF-EMFs, the concentration of TOS was higher in the large intestine than in control rats. The main difference of antioxidant activity was presented by decreased Cu,Zn-SOD in homogenates of the salivary glands, stomach, small and large intestine as well as CAT in homogenates of the tongue, esophagus, stomach, and small and large intestine. Moreover, in rats exposed simultaneously to LF-EMFs and RF-EMFs, a lower concentration of TOS was observed. Antioxidant activity was presented by a decreased activity of CAT in homogenates of the tongue, esophagus, stomach, and small and large intestine in comparison to the control group.Conclusion: Among those applied in the study, electromagnetic fields of a low-frequency caused the most significant disturbances of oxidative stress in the rat gastrointestinal tract.

scholarly journals A comparative study of phytate hydrolysis in the gastrointestinal tract of the golden hamster (Mesocricetus auratus) and the laboratory rat

1985 ◽  
Vol 54 (2) ◽  
pp. 429-435 ◽  
Author(s):  
P. J. Williams ◽  
T. G. Taylor
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Comparative Study ◽  
Large Intestine ◽  
Chromium Oxide ◽  
Golden Hamster ◽  
Solid Phase ◽  
Phytate Hydrolysis ◽  
Hydrolysis Of

1. The role of bacterial, dietary and intestinal phytases (EC 3. 1. 3. 8) in the hydrolysis of phytate was investigated in the golden hamster and rat by assaying phytase in the small intestine and by measuring the disappearance of phytate from the stomach and large intestine, using chromium oxide as an insoluble solid-phase marker.2. It was confirmed that an active phytase was present in the proximal third of the small intestine of the rat but the enzyme was undetectable in the hamster.3. Extensive bacterial breakdown of phytate occurred in the pregastric pouch and true stomach of the hamster with both phytase-containing and phytase-free diets, with phytate digestibilities in the true stomach ranging from 0.69–0.90, confirming that the hamster can be regarded as a pseudo-ruminant.4. With a phytase-free diet, the digestibility of phytate in the stomach of the rat was very low (0.05) but with a wheat-based diet substantial breakdown of phytate occurred (digestibility up to 0.49), presumably under the influence of the cereal phytase.5. Intestinal phytase did not appear to be of great significance in the rat but some further hydrolysis of the residual phytate probably occurred in the large intestine of both species by bacterial phytase.

Description of the gastrointestinal tract and associated organs of the kultarr (Antechinomys laniger)

2013 ◽  
Vol 35 (1) ◽  
pp. 39 ◽  
Author(s):  
Hayley J. Stannard ◽  
Julie M. Old
Keyword(s):  
Gastrointestinal Tract ◽  
Digestive Tract ◽  
Large Intestine ◽  
Cell Types ◽  
Post Mortem ◽  
Microscopic Description ◽  
Future Studies ◽  
New Information ◽  
Small And Large Intestine

This paper provides a macro- and microscopic description of the digestive tract of the kultarr (Antechinomys laniger), a small dasyurid marsupial. The digestive tract was simple, with no external differentiation between the small and large intestine, and lacked a caecum. Mean gross length of the kultarr digestive tract was 165.2 ± 32.1 mm. Microscopically, the tissues had cell types similar to those of other mammals. The new information will aid future post-mortem investigations of captive kultarrs and future studies of nutrition.

Gastrointestinal physiology

2017 ◽  
Author(s):  
Mark Harrison
Keyword(s):  
Emergency Medicine ◽  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Large Intestine ◽  
Functional Anatomy ◽  
Gastrointestinal Physiology

This chapter describes gastrointestinal physiology as it applies to Emergency Medicine, and in particular the Primary FRCEM examination. The chapter outlines the key details of the functional anatomy of the gastrointestinal tract, saliva, swallowing, stomach, small intestine, pancreas, liver, gallbladder, and large intestine. This chapter is laid out exactly following the RCEM syllabus, to allow easy reference and consolidation of learning.

The role of the large intestine in the utilization of feeds containing non-starch polysaccharides

1991 ◽  
Vol 1991 ◽  
pp. 134-134
Author(s):  
A.C. Longland ◽  
W.H. Close ◽  
A.G. Low
Keyword(s):  
Sugar Beet ◽  
Large Intestine ◽  
Growing Pigs ◽  
Sugar Beet Pulp ◽  
Gut Microflora ◽  
End Products ◽  
Beet Pulp ◽  
Hind Gut ◽  
Small And Large Intestine

The utilization of diets high in fibre (defined here as non-starch polysaccharide - NSP) is dependent on the extent to which the NSP is fermented by the gut microflora, and the subsequent utilization by the animal of the end-products of fermentation - the VFAs. It has frequently been assumed that fermentation of NSP in the pig occurs almost exclusively in the hind-gut. However, a number of studies using pigs fitted with ileal-cannulas have suggested that some fermentation of NSP may occur prior to the hind-gut (e.g. Graham et al., 1985). The aim of this study was to determine the relative roles of the small and large intestine in a) the digestion of feeds containing non-starch polysaccharides, and b) the subsequent utilization of energy by growing pigs. This was achieved by comparing the abilities of intact or ileo-rectomised pigs to digest and grow on cereal-based diets containing 0 or 300 g/kg sugar beet pulp.

scholarly journals Contribution of rat liver and gastrointestinal tract to whole-body protein synthesis in the rat

1980 ◽  
Vol 186 (1) ◽  
pp. 381-383 ◽  
Author(s):  
M A McNurlan ◽  
P J Garlick
Keyword(s):  
Protein Synthesis ◽  
Gastrointestinal Tract ◽  
Rat Liver ◽  
Large Intestine ◽  
Whole Body ◽  
Body Protein ◽  
Small And Large Intestine

The rate of protein synthesis was assessed in liver, stomach, small and large intestine and in the whole body of rats by injection of 100 mumol of [14C]leucine/100 g body wt. In each of the tissues turnover was very rapid, so that taken together they accounted for 43% of the protein synthesized by the whole animal.

scholarly journals THE EFFECT OF VARYING LEVELS OF RAW LIMA BEAN (Phasealus lunatus) ON THE RAT'S PANCREATIC AND INTESTINAL TRYPSIN (EC 3.4.21.4) AND CHYMOTRYPSIN (EC 3.4.21.1) ACTIVITIES

2021 ◽  
Vol 11 (2) ◽  
pp. 140-147
Author(s):  
V. A. Aletor ◽  
B. L. Fetuga
Keyword(s):  
Small Intestine ◽  
Large Intestine ◽  
Lima Bean ◽  
Trypsin Activity ◽  
Growing Rats ◽  
Multiple Determination ◽  
Proteinase Inhibition ◽  
Small And Large Intestine ◽  
Pancreatic Proteinase

Two series of assays involving a total of 120 growing rats were carried out to investigate the effect of varying levels of raw lima bean (RLB) on pancreatic and intestinal trypsin EC 3.4.21.4) and chymotrypsin (EC 3.4.21.1) activities. Experiment one indicates significant (P<0.01) inhibtion of both pancreatic trypsin and chymotrypsin due to RLB feeding. Enzyme activities in both the small and large intestine were also significantly (P<0.01) depressed while enzyme values in the caecum were not. Age x Diet interaction was non significant with respect to these enzymes. Pancreatic trypsin and chymotrypsin correlated significantly (P<0.01; P<0.05) with RLB with respective R2, coefficient of multiple determination, of 0.94 and 0.67. Trypsin activity in both the small and large intestine was also significantly (P<0.01) correlated with respective R2 of 0.78 and 0.96. The second study suggests a less than 10% replacement of cooked lima bean by the raw to avert significant pancreatic proteinase inhibition, and a less than 15% replacement of the cooked lima bean by the raw to aver t significantinhibition of the proteinases especially in the small intestine.

scholarly journals Clinical and experimental substantiation of the effect of hypoxia on the wall of the small and large intestine in newborns

2018 ◽  
Vol 11 (4) ◽  
pp. 268-274
Author(s):  
Irina Yuryevna Karpova ◽  
Vyacheslav Vladimirovich Parshikov ◽  
Natalia Nikolaevna Prodanets ◽  
Tatyana Ivanovna Solovieva ◽  
Evgenia Dmitrievna Pyatova ◽  
...  
Keyword(s):  
Small Intestine ◽  
Large Intestine ◽  
Muscle Layer ◽  
Intestinal Wall ◽  
Case Histories ◽  
Tissue Component ◽  
White Rats ◽  
Connective Tissue Component ◽  
Small And Large Intestine ◽  
Experimental Substantiation

Based on the analysis of morphological and morphometric data, the clinical and experimental results of the effect of hypoxia on the wall of the small and large intestine in newborns are presented. The clinical part is based on the study of 58 case histories of patients with necrotizing enterocolitis, which the operating material (resected sections of the small intestine, large intestine) was studied in detail. The experimental work included the modeling of chronic hypobaric hypoxia in different trimesters of pregnancy in 4 groups of white rats (24 females). The offspring was taken out of the experiment at 4-5 days after birth, followed by collection of the small and large intestine areas to study histoarchitectonics. It is proved that hypoxia affects the number of newborn offspring, its weight. The small intestine is most vulnerable to the effect of this factor. Analysis of the morphological and morphometric picture of the operating and experimental material convincingly proves that under the influence of hypoxia the destruction of the intestinal mucosa occurs and the growth of the connective tissue component in the muscle layer occurs. Changes from the microcirculation of blood and lymph provoke the development of pre-ulcerative, ulcerative defects and the formation of necrosis. Disturbance of the drainage function promotes the spread of destruction in the intestinal wall.

scholarly journals 7. Effect of Soya Wastes Probiotic and Palm Kernel Addition Fermented by Aspergillus niger to the Cellulase Activity in Broilers Digestive Tract

2019 ◽  
Vol 13 (1) ◽  
pp. 47-54
Author(s):  
Nurliana Nurliana ◽  
Anna Farida ◽  
Sugito Sugito ◽  
Al Azhar ◽  
Razali Razali ◽  
...  
Keyword(s):  
Small Intestine ◽  
Digestive Tract ◽  
Large Intestine ◽  
Broiler Chickens ◽  
Palm Kernel ◽  
Cellulase Activity ◽  
Feed Supplement ◽  
Concentration Data ◽  
Two Factors ◽  
Small And Large Intestine

This study aimed to determine the cellulase activity in broilers digestive tract after the treatment of soya wastes and palm kernel fermented by A.niger (AKBIS Prob) in the ration. This study used a complete randomized factorial design, which consists of two factors, namely the AKBIS Prob composition factor and the duration of AKBIS Prob. Twenty four broilers were divided into four groups by randomized and three replications of each. The feed supplement combinations were P0 (commercial), P1 (commercial and AKBIS Prob 2%), P2 (commercial and AKBIS Prob 4%), and P3 (commercial and AKBIS Prob 6%). The intestine preparation was taken at the 22 and 36 days to extraction and detection the cellulase activity concentration. Data were analyzed using variance analysis of factorial patterns. AKBIS Prob 2,4 and 6% was no effect (P 0.05) to the cellulase activity in the small and large intestine, while the treatment duration was significant (P0,05) in the small intestine but neither in the large intestine (P0,05) to the cellulase activity. The addition of AKBIS Prob 2.4 and 6% had no effect (P 0.05) on the concentration of cellulase activity in the small intestine and large intestine, while the duration the treatment was very significant (P 0.05) in the small intestine and neither in the large intestine (P 0.05) on the concentration of cellulase activity. The AKBIS administration in the ration for 22 days can increase the activity and concentration of cellulase activity in the small intestine of the digestive tract of broiler chickens.

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