scholarly journals Analysis of Electric Fields inside Microchannels and Single Cell Electrical Lysis with a Microfluidic Device

Micromachines ◽  
2013 ◽  
Vol 4 (2) ◽  
pp. 243-256 ◽  
Author(s):  
Bashir Morshed ◽  
Maitham Shams ◽  
Tofy Mussivand
Keyword(s):  
Single Cell ◽  
Electric Fields ◽  
Microfluidic Device ◽  
Electrical Lysis

A microfluidic device enabling deterministic single cell trapping and release

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Huichao Chai ◽  
Yongxiang Feng ◽  
Fei Liang ◽  
Wenhui Wang
Keyword(s):  
Chemical Analysis ◽  
Single Cell ◽  
Microfluidic Device ◽  
Single Cells ◽  
Cell Isolation ◽  
Cell Trapping ◽  
Analysis Techniques ◽  
Single Cell Trapping ◽  
Single Cell Isolation ◽  
Made In

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...

High-content screening of drug-induced cardiotoxicity using quantitative single cell imaging cytometry on microfluidic device

Lab on a Chip ◽  
2011 ◽  
Vol 11 (1) ◽  
pp. 104-114 ◽  
Author(s):  
Min Jung Kim ◽  
Su Chul Lee ◽  
Sukdeb Pal ◽  
Eunyoung Han ◽  
Joon Myong Song
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Cell Imaging ◽  
High Content Screening ◽  
Drug Induced ◽  
Single Cell Imaging ◽  
Imaging Cytometry

scholarly journals Electrophoretic Concentration and Electrical Lysis of Bacteria in a Microfluidic Device Using a Nanoporous Membrane

Micromachines ◽  
2017 ◽  
Vol 8 (2) ◽  
pp. 45 ◽  
Author(s):  
Md. Islam ◽  
Ali Shahid ◽  
Kacper Kuryllo ◽  
Yingfu Li ◽  
M. Deen ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Nanoporous Membrane ◽  
Electrophoretic Concentration ◽  
Electrical Lysis

scholarly journals Single cell array impedance analysis in a microfluidic device

2016 ◽  
Vol 757 ◽  
pp. 012010
Author(s):  
Emre Altinagac ◽  
Selen Taskin ◽  
Huseyin Kizil
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Impedance Analysis ◽  
Cell Array

scholarly journals Micropipette-Based Microfluidic Device for Monodisperse Microbubbles Generation

Micromachines ◽  
2018 ◽  
Vol 9 (8) ◽  
pp. 387
Author(s):  
Carlos Toshiyuki Matsumi ◽  
Wilson José da Silva ◽  
Fábio Kurt Schneider ◽  
Joaquim Miguel Maia ◽  
Rigoberto E. M. Morales ◽  
...  
Keyword(s):  
Electric Fields ◽  
Microfluidic Device ◽  
Soft Lithography ◽  
Low Cost ◽  
Characteristic Curve ◽  
Microfluidic Devices ◽  
Average Diameter ◽  
Internal Diameter ◽  
Medical Diagnostic ◽  
Average Size

Microbubbles have various applications including their use as carrier agents for localized delivery of genes and drugs and in medical diagnostic imagery. Various techniques are used for the production of monodisperse microbubbles including the Gyratory, the coaxial electro-hydrodynamic atomization (CEHDA), the sonication methods, and the use of microfluidic devices. Some of these techniques require safety procedures during the application of intense electric fields (e.g., CEHDA) or soft lithography equipment for the production of microfluidic devices. This study presents a hybrid manufacturing process using micropipettes and 3D printing for the construction of a T-Junction microfluidic device resulting in simple and low cost generation of monodisperse microbubbles. In this work, microbubbles with an average size of 16.6 to 57.7 μm and a polydispersity index (PDI) between 0.47% and 1.06% were generated. When the device is used at higher bubble production rate, the average diameter was 42.8 μm with increased PDI of 3.13%. In addition, a second-order polynomial characteristic curve useful to estimate micropipette internal diameter necessary to generate a desired microbubble size is presented and a linear relationship between the ratio of gaseous and liquid phases flows and the ratio of microbubble and micropipette diameters (i.e., Qg/Ql and Db/Dp) was found.

scholarly journals Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
B. Deng ◽  
X. F. Li ◽  
D. Y. Chen ◽  
L. D. You ◽  
J. B. Wang ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Single Cell Analysis ◽  
Fine Tuning ◽  
Trapping Efficiency ◽  
Cell Analysis ◽  
Cell Trapping ◽  
Micro Channels ◽  
Single Cell Trapping ◽  
Trapping Sites

Microfluidic cell-based arraying technology is widely used in the field of single-cell analysis. However, among developed devices, there is a compromise between cellular loading efficiencies and trapped cell densities, which deserves further analysis and optimization. To address this issue, the cell trapping efficiency of a microfluidic device with two parallel micro channels interconnected with cellular trapping sites was studied in this paper. By regulating channel inlet and outlet status, the microfluidic trapping structure can mimic key functioning units of previously reported devices. Numerical simulations were used to model this cellular trapping structure, quantifying the effects of channel on/off status and trapping structure geometries on the cellular trapping efficiency. Furthermore, the microfluidic device was fabricated based on conventional microfabrication and the cellular trapping efficiency was quantified in experiments. Experimental results showed that, besides geometry parameters, cellular travelling velocities and sizes also affected the single-cell trapping efficiency. By fine tuning parameters, more than 95% of trapping sites were taken by individual cells. This study may lay foundation in further studies of single-cell positioning in microfluidics and push forward the study of single-cell analysis.

A valve-based microfluidic device for on-chip single cell treatments

2018 ◽  
Vol 40 (6) ◽  
pp. 961-968 ◽  
Author(s):  
Yue Sun ◽  
Bo Cai ◽  
Xiaoyun Wei ◽  
Zixiang Wang ◽  
Lang Rao ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
On Chip
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