A valve-based microfluidic device for on-chip single cell treatments

2018 ◽  
Vol 40 (6) ◽  
pp. 961-968 ◽  
Author(s):  
Yue Sun ◽  
Bo Cai ◽  
Xiaoyun Wei ◽  
Zixiang Wang ◽  
Lang Rao ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
On Chip

scholarly journals Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor †

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1178 ◽  
Author(s):  
Jorge Prada ◽  
Christina Cordes ◽  
Carsten Harms ◽  
Walter Lang
Keyword(s):  
Microfluidic Device ◽  
Heating Temperature ◽  
Low Cost ◽  
Reaction Chamber ◽  
Microfluidic System ◽  
Heating Process ◽  
Design And Manufacturing ◽  
On Chip ◽  
Working Parameters ◽  
Auto Fluorescence

This contribution outlines the design and manufacturing of a microfluidic device implemented as a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval was carried on after bacteria heat-lysis by an on-chip micro-heater, whose function was characterized at different working parameters. Carbon resistive temperature sensors were tested, characterized and printed on the biochip sealing film to monitor the heating process. Off-chip and on-chip processed RNA were hybridized with capture probes on the reaction chamber surface and identification was achieved by detection of fluorescence tags. The application of the mentioned techniques and materials proved to allow the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the integration potential of fully thermoplastic devices in biosensor systems.

A microfluidic device enabling deterministic single cell trapping and release

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Huichao Chai ◽  
Yongxiang Feng ◽  
Fei Liang ◽  
Wenhui Wang
Keyword(s):  
Chemical Analysis ◽  
Single Cell ◽  
Microfluidic Device ◽  
Single Cells ◽  
Cell Isolation ◽  
Cell Trapping ◽  
Analysis Techniques ◽  
Single Cell Trapping ◽  
Single Cell Isolation ◽  
Made In

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...

On-Chip Cell Sorter for Single Cell Expression Analysis

2001 ◽  
pp. 271-273 ◽  
Author(s):  
T. Ichiki ◽  
T. Ujiie ◽  
T. Hara ◽  
Y. Horiike ◽  
K. Yasuda
Keyword(s):  
Single Cell ◽  
Expression Analysis ◽  
Cell Sorter ◽  
On Chip ◽  
Cell Expression

High-content screening of drug-induced cardiotoxicity using quantitative single cell imaging cytometry on microfluidic device

Lab on a Chip ◽  
2011 ◽  
Vol 11 (1) ◽  
pp. 104-114 ◽  
Author(s):  
Min Jung Kim ◽  
Su Chul Lee ◽  
Sukdeb Pal ◽  
Eunyoung Han ◽  
Joon Myong Song
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Cell Imaging ◽  
High Content Screening ◽  
Drug Induced ◽  
Single Cell Imaging ◽  
Imaging Cytometry

On-Chip Single-Cell Lysis for Extracting Intracellular Material

2007 ◽  
Vol 46 (9B) ◽  
pp. 6410-6414 ◽  
Author(s):  
Norifumi Ikeda ◽  
Nobuaki Tanaka ◽  
Yasuko Yanagida ◽  
Takeshi Hatsuzawa
Keyword(s):  
Single Cell ◽  
Cell Lysis ◽  
On Chip

scholarly journals An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in droplets with single cell resolution

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Mathias Girault ◽  
Hyonchol Kim ◽  
Hisayuki Arakawa ◽  
Kenji Matsuura ◽  
Masao Odaka ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Shape Recognition ◽  
Target Cells ◽  
Recognition Method ◽  
System A ◽  
Time Shape ◽  
Droplet Sorting ◽  
On Chip ◽  
Sorting System

scholarly journals Single cell array impedance analysis in a microfluidic device

2016 ◽  
Vol 757 ◽  
pp. 012010
Author(s):  
Emre Altinagac ◽  
Selen Taskin ◽  
Huseyin Kizil
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Impedance Analysis ◽  
Cell Array

scholarly journals Massively parallel quantification of phenotypic heterogeneity in single cell drug responses

2020 ◽  
Author(s):  
Benjamin B. Yellen ◽  
Jon S. Zawistowski ◽  
Eric A. Czech ◽  
Caleb I. Sanford ◽  
Elliott D. SoRelle ◽  
...  
Keyword(s):  
Machine Learning ◽  
Acute Myeloid Leukemia ◽  
Single Cell ◽  
Myeloid Leukemia ◽  
Single Cell Analysis ◽  
Phenotypic Heterogeneity ◽  
Massively Parallel ◽  
Cell Analysis ◽  
On Chip ◽  
Acute Myeloid

AbstractSingle cell analysis tools have made significant advances in characterizing genomic heterogeneity, however tools for measuring phenotypic heterogeneity have lagged due to the increased difficulty of handling live biology. Here, we report a single cell phenotyping tool capable of measuring image-based clonal properties at scales approaching 100,000 clones per experiment. These advances are achieved by exploiting a novel flow regime in ladder microfluidic networks that, under appropriate conditions, yield a mathematically perfect cell trap. Machine learning and computer vision tools are used to control the imaging hardware and analyze the cellular phenotypic parameters within these images. Using this platform, we quantified the responses of tens of thousands of single cell-derived acute myeloid leukemia (AML) clones to targeted therapy, identifying rare resistance and morphological phenotypes at frequencies down to 0.05%. This approach can be extended to higher-level cellular architectures such as cell pairs and organoids and on-chip live-cell fluorescence assays.

Sign in / Sign up

Export Citation Format

Share Document