scholarly journals Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 132
Author(s):  
Mohammadhossein Dabaghi ◽  
Shadi Shahriari ◽  
Neda Saraei ◽  
Kevin Da ◽  
Abiram Chandiramohan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Surface Modification ◽  
Cell Adhesion ◽  
Cell Attachment ◽  
Microfluidic Devices ◽  
Bronchial Epithelial ◽  
Ecm Proteins ◽  
Coating Methods ◽  
Organ On A Chip

Polydimethylsiloxane (PDMS) is a silicone-based synthetic material used in various biomedical applications due to its properties, including transparency, flexibility, permeability to gases, and ease of use. Though PDMS facilitates and assists the fabrication of complicated geometries at micro- and nano-scales, it does not optimally interact with cells for adherence and proliferation. Various strategies have been proposed to render PDMS to enhance cell attachment. The majority of these surface modification techniques have been offered for a static cell culture system. However, dynamic cell culture systems such as organ-on-a-chip devices are demanding platforms that recapitulate a living tissue microenvironment’s complexity. In organ-on-a-chip platforms, PDMS surfaces are usually coated by extracellular matrix (ECM) proteins, which occur as a result of a physical and weak bonding between PDMS and ECM proteins, and this binding can be degraded when it is exposed to shear stresses. This work reports static and dynamic coating methods to covalently bind collagen within a PDMS-based microfluidic device using polydopamine (PDA). These coating methods were evaluated using water contact angle measurement and atomic force microscopy (AFM) to optimize coating conditions. The biocompatibility of collagen-coated PDMS devices was assessed by culturing primary human bronchial epithelial cells (HBECs) in microfluidic devices. It was shown that both PDA coating methods could be used to bind collagen, thereby improving cell adhesion (approximately three times higher) without showing any discernible difference in cell attachment between these two methods. These results suggested that such a surface modification can help coat extracellular matrix protein onto PDMS-based microfluidic devices.

scholarly journals Surface modification of PDMS-based microfluidic devices with collagen using polydopamine as a spacer to enhance primary human bronchial epithelial cell adhesion

2020 ◽  
Author(s):  
Mohammadhossein Dabaghi ◽  
Shadi Shahriari ◽  
Neda Saraei ◽  
Kevin Da ◽  
Abiram Chandiramohan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Surface Modification ◽  
Cell Adhesion ◽  
Microfluidic Devices ◽  
Extracellular Matrix Protein ◽  
Shear Stresses ◽  
Bronchial Epithelial ◽  
Ecm Proteins ◽  
Coating Methods ◽  
Organ On A Chip

AbstractPolydimethylsiloxane (PDMS) is a silicone-based synthetic material that is used in various biomedical applications due to its properties, including transparency, flexibility, permeability to gases, and ease of use. Though PDMS facilitates and realizes the fabrication of complicated geometries at the micro and nano scales, it does not optimally interact with cells for adherence and proliferation. Different strategies have been proposed to render PDMS to enhance cell attachment. The majority of these surface modification techniques have been offered for a static cell culture system. However, dynamic cell culture systems such as organ-on-a-chip devices are demanding platforms that recapitulate the complexity of a living tissue microenvironment. For organ-on-a-chip platforms, PDMS surfaces are usually coated by ECM proteins, which occur as a result of physical, weak bonding between PDMS and ECM proteins, and this binding can be degraded when it is exposed to shear stresses. This work reports static and dynamic coating methods to covalently bind collagen within a PDMS-based microfluidic device using polydopamine (PDA). These coating methods were evaluated using water contact angle measurement and atomic force microscopy (AFM) to find the optimum coating conditions. The biocompatibility of collagen-coated PDMS devices was assessed by culturing primary human bronchial epithelial cells (HBECs) in microfluidic devices. It was shown that both PDA coating methods could be used to bind collagen, thereby improving cell adhesion (around three times higher) without showing any discernible difference. These results suggested that such a surface modification can be used to coat an extracellular matrix protein onto PDMS-based microfluidic devices.

Regulatory effects of stem cell factor and interleukin-4 on adhesion of human mast cells to extracellular matrix proteins

Blood ◽  
2002 ◽  
Vol 99 (3) ◽  
pp. 966-972 ◽  
Author(s):  
Axel Lorentz ◽  
Detlef Schuppan ◽  
Andreas Gebert ◽  
Michael P. Manns ◽  
Stephan C. Bischoff
Keyword(s):  
Extracellular Matrix ◽  
Mast Cell ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Mast Cells ◽  
Stem Cell Factor ◽  
Collagen I ◽  
Interleukin 4 ◽  
Human Mast Cells ◽  
Ecm Proteins

Abstract Mast cells are inflammatory and immunoregulatory cells resident in tissues. They develop from bone marrow-derived progenitor cells that enter the tissue through the blood circulation. The specific localization and migration of mast cells in tissues is dependent on their interaction with extracellular matrix (ECM) proteins. Adhesion of human mast cells isolated from intestinal mucosa and cultured in the presence of stem cell factor (SCF) to ECM proteins is analyzed. It was observed that SCF is a unique cytokine enhancing mast cell adhesion to all tested ECM proteins (fibronectin, laminin, collagen I, III, IV, VI, XIV) up to 5-fold, particularly to fibronectin (54% ± 12% of mast cells) and to denatured collagens (40% ± 12% on cyanogen bromide-cleaved peptides of collagen I). Most noteworthy, preculture of mast cells with interleukin-4 (IL-4), in addition to SCF, reduced their potency to adhere to ECM proteins to one third compared to mast cells cultured with SCF alone. Mast cell adhesion was preferentially mediated by β1 integrins, and most cells expressed the ECM-binding integrins α2β1, α3β1, α4β1, α5β1, and αVβ3. SCF-induced mast cell adhesion was totally blocked by wortmannin and apigenin, indicating an involvement of phosphatidylinositol 3-kinase and mitogen-activated protein kinase, and it was related to an up-regulation of the HUTS-21 β1 epitope, which is associated with an activated conformation of β1. In conclusion, these data indicate that SCF induces the adhesion of cultured mast cells to ECM proteins, whereas IL-4 may promote detachment from the ECM.

Laminin-511 and recombinant vitronectin supplementation enables human pluripotent stem cell culture and differentiation on conventional tissue culture polystyrene surfaces in xeno-free conditions

2021 ◽  
Author(s):  
Ya-Chu Liu ◽  
Lee-Kiat Ban ◽  
Henry Hsin-Chung Lee ◽  
Hsin-Ting Lee ◽  
Yu-Tang Chang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Extracellular Matrix ◽  
Tissue Culture ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Culture Conditions ◽  
Human Pluripotent Stem Cell ◽  
Tissue Culture Polystyrene ◽  
Ecm Proteins

Human pluripotent stem cells (hPSCs) are typically cultivated on extracellular matrix (ECM) protein-coated dishes in xeno-free culture conditions. We supplemented mixed ECM proteins (laminin-511 and recombinant vitronectin, rVT) in culture...

Development of a Plasma Process for Microfluidic Devices in the Prospect of Cell Attachment

2010 ◽  
Vol 89-91 ◽  
pp. 598-603
Author(s):  
Nathalie Vo Tan Tho ◽  
Hervé Willaime ◽  
Patrick Tabeling ◽  
Farzaneh Arefi-Khonsari ◽  
D. Mantovani ◽  
...  
Keyword(s):  
Surface Modification ◽  
Vapor Deposition ◽  
Low Temperatures ◽  
Cell Attachment ◽  
Chemical Vapor ◽  
Microfluidic Devices ◽  
Microfluidic System ◽  
Plasma Process ◽  
Dry Process ◽  
Inner Surface

Plasma processing has been developed to produce selective chemistry in the inner surface of a microfluidic system. This dry process is an alternative solution to the Chemical Vapor Deposition (CVD) process that allows us to work at low temperatures thus avoiding the degradation of the substrate by heat. The present study focused on the surface modification of PDMS in order to make them more hydrophilic and capable to exhibit a high percentage of COOH functions which will provide a good asset for future cell attachment.

Geometrically customizable alginate hydrogel nanofibers for cell culture platforms

2019 ◽  
Vol 7 (42) ◽  
pp. 6556-6563 ◽  
Author(s):  
Satoshi Fujita ◽  
Yuka Wakuda ◽  
Minori Matsumura ◽  
Shin-ichiro Suye
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Core Shell ◽  
Anisotropic Structure ◽  
Alginate Hydrogel ◽  
Cell Adhesion And Proliferation

Hydrogel nanofibers derived from alginate with an anisotropic structure fabricated by using core–shell electrospinning play an important role in cell adhesion and proliferation as the extracellular matrix (ECM).

scholarly journals Enhanced cell attachment using a novel cell culture surface presenting functional domains from extracellular matrix proteins

2008 ◽  
Vol 56 (2) ◽  
pp. 71-79 ◽  
Author(s):  
M. J. Cooke ◽  
S. R. Phillips ◽  
D. S. H. Shah ◽  
D. Athey ◽  
J. H. Lakey ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Cell Attachment ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Functional Domains ◽  
Culture Surface

scholarly journals Matrix Valency Regulates Integrin-mediated Lymphoid Adhesion via Syk Kinase

1999 ◽  
Vol 144 (4) ◽  
pp. 777-788 ◽  
Author(s):  
Dwayne G. Stupack ◽  
Erguang Li ◽  
Steve A. Silletti ◽  
Jacqueline A. Kehler ◽  
Robert L. Geahlen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Lymphoid Cell ◽  
Lymphoid Cells ◽  
Cooperative Interaction ◽  
Antigen Receptors ◽  
Adhesion Proteins ◽  
Adhesion Sites ◽  
Syk Kinase ◽  
Ecm Proteins

Lymphocytes accumulate within the extracellular matrix (ECM) of tumor, wound, or inflammatory tissues. These tissues are largely comprised of polymerized adhesion proteins such as fibrin and fibronectin or their fragments. Nonactivated lymphoid cells attach preferentially to polymerized ECM proteins yet are unable to attach to monomeric forms or fragments of these proteins without previous activation. This adhesion event depends on the appropriate spacing of integrin adhesion sites. Adhesion of nonactivated lymphoid cells to polymeric ECM components results in activation of the antigen receptor-associated Syk kinase that accumulates in adhesion-promoting podosomes. In fact, activation of Syk by antigen or agonists, as well as expression of an activated Syk mutant in lymphoid cells, facilitates their adhesion to monomeric ECM proteins or their fragments. These results reveal a cooperative interaction between signals emanating from integrins and antigen receptors that can serve to regulate stable lymphoid cell adhesion and retention within a remodeling ECM.

Binding of hexabrachion (tenascin) to the extracellular matrix and substratum and its effect on cell adhesion

1990 ◽  
Vol 95 (2) ◽  
pp. 263-277
Author(s):  
V.A. Lightner ◽  
H.P. Erickson
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Cell Attachment ◽  
Solid Phase ◽  
Fluid Phase ◽  
Matrix Proteins ◽  
And Migration ◽  
Cover Up ◽  
Plastic Substratum

Hexabrachion is a large glycoprotein of the extracellular matrix (ECM) that is prominent in embryogenesis, wound healing and tumorigenesis. Because of the role of extracellular matrix proteins in the regulation of cell differentiation and migration, the interaction of hexabrachion with cells as well as with other components of the ECM is of great interest. Early reports suggested that hexabrachion does not bind to fibronectin or gelatin but does bind to chondroitin sulfate proteoglycans. However, more recent reports have suggested that hexabrachion binds to fibronectin and inhibits cell adhesion as well as cell migration on fibronectin. We have found no evidence of strong hexabrachion-fibronectin binding on either a solid-phase ELISA assay or in a fluid-phase sedimentation assay in which the reactants were allowed to dissociate. However, hexabrachion sedimentation was accelerated in a gradient containing fibronectin throughout. This demonstrates an association between hexabrachions and fibronectin, but the complex is apparently weak and readily reversible. The solid-phase ELISA also shows no evidence of hexabrachion binding to gelatin, laminin or types I, III, IV or V collagen. Hexabrachion does not support strong cell attachment of the cell lines tested. Moreover, hexabrachion can inhibit cell attachment to fibronectin. We demonstrate here that this inhibition requires the hexabrachion to be able to bind to the plastic substratum. The results suggest that hexabrachion inhibition is via a steric inhibition. When the hexabrachion molecules bind to the plastic, they cover up a significant fraction of the underlying fibronectin molecules. Antibody studies are presented that show that hexabrachion can nonspecifically block access of immunoglobulin G molecules to the underlying matrix. This steric blocking is not unique to hexabrachion.

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