Geometrically customizable alginate hydrogel nanofibers for cell culture platforms

2019 ◽  
Vol 7 (42) ◽  
pp. 6556-6563 ◽  
Author(s):  
Satoshi Fujita ◽  
Yuka Wakuda ◽  
Minori Matsumura ◽  
Shin-ichiro Suye
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Core Shell ◽  
Anisotropic Structure ◽  
Alginate Hydrogel ◽  
Cell Adhesion And Proliferation

Hydrogel nanofibers derived from alginate with an anisotropic structure fabricated by using core–shell electrospinning play an important role in cell adhesion and proliferation as the extracellular matrix (ECM).

Peptide-modified conducting polymer as a biofunctional surface: monitoring of cell adhesion and proliferation

RSC Advances ◽  
2014 ◽  
Vol 4 (96) ◽  
pp. 53411-53418 ◽  
Author(s):  
Gizem Oyman ◽  
Caner Geyik ◽  
Rukiye Ayranci ◽  
Metin Ak ◽  
Dilek Odaci Demirkol ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Conducting Polymer ◽  
Functional Surface ◽  
Promising Candidate ◽  
Surface Monitoring ◽  
Cell Adhesion And Proliferation

A designed bio-functional surface is a promising candidate forcell-culture-on-a-chipapplications.

scholarly journals Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 132
Author(s):  
Mohammadhossein Dabaghi ◽  
Shadi Shahriari ◽  
Neda Saraei ◽  
Kevin Da ◽  
Abiram Chandiramohan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Surface Modification ◽  
Cell Adhesion ◽  
Cell Attachment ◽  
Microfluidic Devices ◽  
Bronchial Epithelial ◽  
Ecm Proteins ◽  
Coating Methods ◽  
Organ On A Chip

Polydimethylsiloxane (PDMS) is a silicone-based synthetic material used in various biomedical applications due to its properties, including transparency, flexibility, permeability to gases, and ease of use. Though PDMS facilitates and assists the fabrication of complicated geometries at micro- and nano-scales, it does not optimally interact with cells for adherence and proliferation. Various strategies have been proposed to render PDMS to enhance cell attachment. The majority of these surface modification techniques have been offered for a static cell culture system. However, dynamic cell culture systems such as organ-on-a-chip devices are demanding platforms that recapitulate a living tissue microenvironment’s complexity. In organ-on-a-chip platforms, PDMS surfaces are usually coated by extracellular matrix (ECM) proteins, which occur as a result of a physical and weak bonding between PDMS and ECM proteins, and this binding can be degraded when it is exposed to shear stresses. This work reports static and dynamic coating methods to covalently bind collagen within a PDMS-based microfluidic device using polydopamine (PDA). These coating methods were evaluated using water contact angle measurement and atomic force microscopy (AFM) to optimize coating conditions. The biocompatibility of collagen-coated PDMS devices was assessed by culturing primary human bronchial epithelial cells (HBECs) in microfluidic devices. It was shown that both PDA coating methods could be used to bind collagen, thereby improving cell adhesion (approximately three times higher) without showing any discernible difference in cell attachment between these two methods. These results suggested that such a surface modification can help coat extracellular matrix protein onto PDMS-based microfluidic devices.

scholarly journals Folic acid modified clay/polymer nanocomposites for selective cell adhesion

2014 ◽  
Vol 2 (37) ◽  
pp. 6412-6421 ◽  
Author(s):  
F. B. Barlas ◽  
D. Ag Seleci ◽  
M. Ozkan ◽  
B. Demir ◽  
M. Seleci ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Folic Acid ◽  
Polymer Nanocomposites ◽  
Promising Material ◽  
Modified Clay ◽  
A Cell ◽  
Cell Adhesion And Proliferation

A promising material, a folic acid modified poly(epsilon-caprolactone)/clay nanocomposite that allows selective cell adhesion and proliferation, was synthesized and characterized as a cell culture and biosensing platform.

Osteogenic capability of strontium and icariin-loaded TiO2 nanotube coatings in vitro and in osteoporotic rats

2021 ◽  
pp. 088532822199799
Author(s):  
Ye Zhu ◽  
Tianxia Zheng ◽  
Li-ming Wen ◽  
Ren Li ◽  
Yan-bo Zhang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Alkaline Phosphatase ◽  
Cell Adhesion ◽  
Titanium Implants ◽  
Ovariectomized Rats ◽  
Alp Activity ◽  
Ti Alloys ◽  
Bone Integration ◽  
Cell Adhesion And Proliferation

Titanium (Ti) and Ti alloys are widely used biomaterials, but they lack osteogenic capability for rapid bone integration. To improve osseointegration of Ti implants, TiO2 nanotubes were prepared using the anodizing oxidation technique, and strontium (Sr) combined with icariin (ICA) was loaded on TiO2 nanotube coatings. Cell adhesion and proliferation of MC3T3-E1 cells, alkaline phosphatase (ALP) activity, mineralization of extracellular matrix, and bone formation around titanium implants in ovariectomized rats, were examined separately. The results showed that compared with pure Ti, TiO2 and Sr-loaded TiO2 coatings, the coatings loaded with both Sr and ICA showed better effect on cell adhesion and proliferation, higher ALP activity and more red-stained mineralized nodules. Furthermore, more bone was formed around implants loaded with both Sr and ICA in osteoporotic rats. Therefore, coating with Sr and ICA is valuable for clinical application to strengthen the osseointegration of titanium implants, especially in osteoporotic patients.

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