scholarly journals Ultrasensitive Stress Biomarker Detection Using Polypyrrole Nanotube Coupled to a Field-Effect Transistor

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 439 ◽  
Author(s):  
Kyung Ho Kim ◽  
Sang Hun Lee ◽  
Sung Eun Seo ◽  
Joonwon Bae ◽  
Seon Joo Park ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistor ◽  
Dynamic Range ◽  
Amide Bond ◽  
Post Traumatic Stress ◽  
Stress Biomarkers ◽  
Bodily Fluids ◽  
Rapid Responses ◽  
Effect Transistor ◽  
Detection Of Stress

Stress biomarkers such as hormones and neurotransmitters in bodily fluids can indicate an individual’s physical and mental state, as well as influence their quality of life and health. Thus, sensitive and rapid detection of stress biomarkers (e.g., cortisol) is important for management of various diseases with harmful symptoms, including post-traumatic stress disorder and depression. Here, we describe rapid and sensitive cortisol detection based on a conducting polymer (CP) nanotube (NT) field-effect transistor (FET) platform. The synthesized polypyrrole (PPy) NT was functionalized with the cortisol antibody immunoglobulin G (IgG) for the sensitive and specific detection of cortisol hormone. The anti-cortisol IgG was covalently attached to a basal plane of PPy NT through an amide bond between the carboxyl group of PPy NT and the amino group of anti-cortisol IgG. The resulting field-effect transistor-type biosensor was utilized to evaluate various cortisol concentrations. Cortisol was sensitively measured to a detection limit of 2.7 × 10−10 M (100 pg/mL), with a dynamic range of 2.7 × 10−10 to 10−7 M; it exhibited rapid responses (<5 s). We believe that our approach can serve as an alternative to time-consuming and labor-intensive health questionnaires; it can also be used for diagnosis of underlying stress-related disorders.

Detection of Stress-Related Secretory IgA in Human Sweat Using Lectin-Immobilized Field Effect Transistor Biosensor

2020 ◽  
Vol MA2020-02 (66) ◽  
pp. 3391-3391
Author(s):  
Hiroki Hayashi ◽  
Naoki Sakamoto ◽  
Sho Hideshima ◽  
Yoshitaka Harada ◽  
Mika Tsuna ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistor ◽  
Secretory Iga ◽  
Human Sweat ◽  
Effect Transistor ◽  
Detection Of Stress

scholarly journals Construction and Application of Graphene Oxide-Bovine Serum Albumin Modified Extended Gate Field Effect Transistor Chiral Sensor

Sensors ◽  
2021 ◽  
Vol 21 (11) ◽  
pp. 3921
Author(s):  
Le Li ◽  
Xiaofei Ma ◽  
Yin Xiao ◽  
Yong Wang
Keyword(s):  
Graphene Oxide ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Chiral Resolution ◽  
Oxide Semiconductor ◽  
Amide Bond ◽  
Effect Transistor

Chirality is an essential natural attribute of organisms. Chiral molecules exhibit differences in biochemical processes, pharmacodynamics, and toxicological properties, and their enantioselective recognition plays an important role in explaining life science processes and guiding drug design. Herein, we developed an ultra-sensitive enantiomer recognition platform based on an extended-gate metal-oxide semiconductor field-effect-transistor (Nafion–GO@BSA–EG-MOSFET) that achieved effective chiral resolution of ultra-sensitive Lysine (Lys) and α-Methylbenzylamine (α-Met) enantiodiscrimination at the femtomole level. Bovine serum albumin (BSA) was immobilized on the surface of graphene oxide (GO) through amide bond coupling to prepare the GO@BSA complex. GO@BSA was drop-cast on deposited Au surfaces with a Nafion solution to afford the extended-gate sensing unit. Effective recognition of chiral enantiomers of mandelic acid (MA), tartaric acid (TA), tryptophan (Trp), Lys and α-Met was realized. Moreover, the introduction of GO reduced non-specific adsorption, and the chiral resolution concentration of α-Met reached the level of picomole in a 5-fold diluted fetal bovine serum (FBS). Finally, the chiral recognition mechanism of the as-fabricated sensor was proposed.

Detection of Stress-Related Secretory IgA in Human Sweat Using Lectin-Immobilized Field Effect Transistor Biosensor

2020 ◽  
Vol MA2020-01 (27) ◽  
pp. 1902-1902
Author(s):  
Hiroki Hayashi ◽  
Naoki Sakamoto ◽  
Sho Hideshima ◽  
Yoshitaka Harada ◽  
Mika Tsuna ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistor ◽  
Secretory Iga ◽  
Human Sweat ◽  
Effect Transistor ◽  
Detection Of Stress

Detection of Protein by Reduced Graphene Oxide Field-Effect Transistor

2014 ◽  
Vol 1586 ◽  
Author(s):  
Masaki Hasegawa ◽  
Yuki Hirayama ◽  
Yasuhide Ohno ◽  
Kenzo Maehashi ◽  
Kazuhiko Matsumoto
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Dynamic Range ◽  
Immunoglobulin E ◽  
Pristine Graphene ◽  
Fluorescent Markers ◽  
Reduced Graphene ◽  
Effect Transistor

ABSTRACTWe confirmed a specific detection of immunoglobulin E(IgE) by using an aptamer immobilized reduced graphene oxide(rGo) field effect transistor(FET). A detection limit and dynamic range were estimated 8.1 ng/ml and 10000 respectively. These characteristics are comparable with current fluorescent markers. Although a mobility of rGo FET was around 5 cm2/V.sec, and this is two to three orders lower than mechanically exfoliated pristine graphene FET, a sensitivity of it was only one order lower than using pristine graphene.

scholarly journals Time Resolution and Dynamic Range of Field-Effect Transistor–Based Terahertz Detectors

2019 ◽  
Vol 40 (7) ◽  
pp. 703-719 ◽  
Author(s):  
Przemyslaw Zagrajek ◽  
Sergey N. Danilov ◽  
Jacek Marczewski ◽  
Michal Zaborowski ◽  
Cezary Kolacinski ◽  
...  
Keyword(s):  
Time Resolution ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Dynamic Range ◽  
Terahertz Detectors ◽  
Effect Transistor

scholarly journals Extended gate field-effect-transistor for sensing cortisol stress hormone

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Shokoofeh Sheibani ◽  
Luca Capua ◽  
Sadegh Kamaei ◽  
Sayedeh Shirin Afyouni Akbari ◽  
Junrui Zhang ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistor ◽  
Dynamic Range ◽  
Voltage Sensitivity ◽  
Debye Screening Length ◽  
Current Sensitivity ◽  
Human Sweat ◽  
Wide Range ◽  
Label Free Detection ◽  
Effect Transistor

AbstractCortisol is a hormone released in response to stress and is a major glucocorticoid produced by adrenal glands. Here, we report a wearable sensory electronic chip using label-free detection, based on a platinum/graphene aptamer extended gate field effect transistor (EG-FET) for the recognition of cortisol in biological buffers within the Debye screening length. The device shows promising experimental features for real-time monitoring of the circadian rhythm of cortisol in human sweat. We report a hysteresis-free EG-FET with a voltage sensitivity of the order of 14 mV/decade and current sensitivity up to 80% over the four decades of cortisol concentration. The detection limit is 0.2 nM over a wide range, between 1 nM and 10 µM, of cortisol concentrations in physiological fluid, with negligible drift over time and high selectivity. The dynamic range fully covers those in human sweat. We propose a comprehensive analysis and a unified, predictive analytical mapping of current sensitivity in all regimes of operation.

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