scholarly journals Differential Sorting of Microparticles Using Spiral Microchannels with Elliptic Configurations

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 412
Author(s):  
Kaan Erdem ◽  
Vahid Ebrahimpour Ahmadi ◽  
Ali Kosar ◽  
Lütfullah Kuddusi
Keyword(s):  
Cell Sorting ◽  
Microfluidic Channel ◽  
Major Axis ◽  
Label Free ◽  
Flow Rates ◽  
Inertial Focusing ◽  
Size Dependent ◽  
Curved Channels ◽  
Aspect Ratios ◽  
Dependent Cell

Label-free, size-dependent cell-sorting applications based on inertial focusing phenomena have attracted much interest during the last decade. The separation capability heavily depends on the precision of microparticle focusing. In this study, five-loop spiral microchannels with a height of 90 µm and a width of 500 µm are introduced. Unlike their original spiral counterparts, these channels have elliptic configurations of varying initial aspect ratios, namely major axis to minor axis ratios of 3:2, 11:9, 9:11, and 2:3. Accordingly, the curvature of these configurations increases in a curvilinear manner through the channel. The effects of the alternating curvature and channel Reynolds number on the focusing of fluorescent microparticles with sizes of 10 and 20 µm in the prepared suspensions were investigated. At volumetric flow rates between 0.5 and 3.5 mL/min (allowing separation), each channel was tested to collect samples at the designated outlets. Then, these samples were analyzed by counting the particles. These curved channels were capable of separating 20 and 10 µm particles with total yields up to approximately 95% and 90%, respectively. The results exhibited that the level of enrichment and the focusing behavior of the proposed configurations are promising compared to the existing microfluidic channel configurations.

scholarly journals Microparticle Inertial Focusing in an Asymmetric Curved Microchannel

Fluids ◽  
2018 ◽  
Vol 3 (3) ◽  
pp. 57 ◽  
Author(s):  
Arzu Özbey ◽  
Mehrdad Karimzadehkhouei ◽  
Hossein Alijani ◽  
Ali Koşar
Keyword(s):  
Biomedical Applications ◽  
Cost Effective ◽  
Label Free ◽  
Promising Technique ◽  
Flow Rates ◽  
Inertial Focusing ◽  
Micro Total Analysis Systems ◽  
Symmetric Geometry ◽  
Total Analysis ◽  
Different Diameters

Inertial Microfluidics offer a high throughput, label-free, easy to design, and cost-effective solutions, and are a promising technique based on hydrodynamic forces (passive techniques) instead of external ones, which can be employed in the lab-on-a-chip and micro-total-analysis-systems for the focusing, manipulation, and separation of microparticles in chemical and biomedical applications. The current study focuses on the focusing behavior of the microparticles in an asymmetric curvilinear microchannel with curvature angle of 280°. For this purpose, the focusing behavior of the microparticles with three different diameters, representing cells with different sizes in the microchannel, was experimentally studied at flow rates from 400 to 2700 µL/min. In this regard, the width and position of the focusing band are carefully recorded for all of the particles in all of the flow rates. Moreover, the distance between the binary combinations of the microparticles is reported for each flow rate, along with the Reynolds number corresponding to the largest distances. Furthermore, the results of this study are compared with those of the microchannel with the same curvature angle but having a symmetric geometry. The microchannel proposed in this study can be used or further modified for cell separation applications.

Continuous Cell Sorting by Dielectrophoresis in a Straight Microfluidic Channel

2018 ◽  
Author(s):  
Yuhao Qiang ◽  
Jia Liu ◽  
Darryl Dieujuste ◽  
Katrina Ramsamooj ◽  
Sarah E. Du
Keyword(s):  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Cell Sorting ◽  
Separation Efficiency ◽  
Microfluidic Channel ◽  
Bottom Surface ◽  
Straight Channel ◽  
Effective Mechanism ◽  
Flow Rates ◽  
High Separation

Dielectrophoresis (DEP) has been demonstrated as an effective mechanism for cell sorting in microfluidic settings. Many existing methods utilize sophisticated microfluidic designs that require complicated fabrication process and operations. In this paper, we present a microfluidics-based cell sorter that is capable of sorting microparticles continuously in a simple straight channel, thus facilitating easier fabrication and operation. An array of indium-tin oxide (ITO) electrodes are embedded on the bottom surface of the straight channel to generate a DEP force field. This force results in deviation of the particles with different dielectric properties from their paths that are hydrodynamically focused in the channel. Particle trajectories are predicted by numerical simulation at different flow rates and field strengths using COMSOL. Separation of red blood cells from polystyrene beads is demonstrated and numerical prediction is validated experimentally. High separation efficiency for the two particle types is confirmed by counting the concentrations of particles collected at the respective collection outlet.

scholarly journals A Numerical Simulation of Cell Separation by Simplified Asymmetric Pinched Flow Fractionation

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Jing-Tao Ma ◽  
Yuan-Qing Xu ◽  
Xiao-Ying Tang
Keyword(s):  
Lattice Boltzmann ◽  
Cell Sorting ◽  
Cell Separation ◽  
Immersed Boundary ◽  
Flow Cells ◽  
Size Dependent ◽  
Sorting Technique ◽  
Dependent Cell ◽  
A Cell ◽  
Boltzmann Method

As a typical microfluidic cell sorting technique, the size-dependent cell sorting has attracted much interest in recent years. In this paper, a size-dependent cell sorting scheme is presented based on a controllable asymmetric pinched flow by employing an immersed boundary-lattice Boltzmann method (IB-LBM). The geometry of channels consists of 2 upstream branches, 1 transitional channel, and 4 downstream branches (D-branches). Simulations are conducted by varying inlet flow ratio, the cell size, and the ratio of flux of outlet 4 to the total flux. It is found that, after being randomly released in one upstream branch, the cells are aligned in a line close to one sidewall of the transitional channel due to the hydrodynamic forces of the asymmetric pinched flow. Cells with different sizes can be fed into different downstream D-branches just by regulating the flux of one D-branch. A principle governing D-branch choice of a cell is obtained, with which a series of numerical cases are performed to sort the cell mixture involving two, three, or four classes of diameters. Results show that, for each case, an adaptive regulating flux can be determined to sort the cell mixture effectively.

scholarly journals Inertial Microfluidics Enabling Clinical Research

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 257
Author(s):  
Srivathsan Kalyan ◽  
Corinna Torabi ◽  
Harrison Khoo ◽  
Hyun Woo Sung ◽  
Sung-Eun Choi ◽  
...  
Keyword(s):  
Microfluidic Channel ◽  
Relevant Information ◽  
Label Free ◽  
Inertial Microfluidics ◽  
Inertial Focusing ◽  
Viscous Forces ◽  
Solution Exchange ◽  
On Chip ◽  
Improving Accuracy ◽  
Hybrid Devices

Fast and accurate interrogation of complex samples containing diseased cells or pathogens is important to make informed decisions on clinical and public health issues. Inertial microfluidics has been increasingly employed for such investigations to isolate target bioparticles from liquid samples with size and/or deformability-based manipulation. This phenomenon is especially useful for the clinic, owing to its rapid, label-free nature of target enrichment that enables further downstream assays. Inertial microfluidics leverages the principle of inertial focusing, which relies on the balance of inertial and viscous forces on particles to align them into size-dependent laminar streamlines. Several distinct microfluidic channel geometries (e.g., straight, curved, spiral, contraction-expansion array) have been optimized to achieve inertial focusing for a variety of purposes, including particle purification and enrichment, solution exchange, and particle alignment for on-chip assays. In this review, we will discuss how inertial microfluidics technology has contributed to improving accuracy of various assays to provide clinically relevant information. This comprehensive review expands upon studies examining both endogenous and exogenous targets from real-world samples, highlights notable hybrid devices with dual functions, and comments on the evolving outlook of the field.

Investigation of the Inertial Focusing Behavior in Curved Microfluidic Channels for Different Aspect Ratio

2016 ◽  
Author(s):  
Alireza Setayesh Hagh ◽  
Ali Dinler
Keyword(s):  
Particle Separation ◽  
Microfluidic Channels ◽  
Flow Conditions ◽  
Specific Flow ◽  
Inertial Focusing ◽  
Particle Focusing ◽  
Curved Channels ◽  
Aspect Ratios ◽  
Specific Number ◽  
Significant Attention

Inertial focusing has attracted a significant attention in microfluidics applications in recent years. Inertial focusing occurs only under specific flow conditions at which particles migrate across streamlines to a specific number of equilibrium positions. This behavior is mostly not sensitive to the particle size in straight channels. However, curved channels can allow sized based particle separation. In this study, curved channels with various aspect ratios have been investigated by numerical simulations. Consideration of flow regimes reveals that some conditions establish a high-quality single-particle focusing situation which is characterized by the alignment of particles within a narrow band. The outcomes of our numerical model contribute to the understanding of limitation of particle focusing and particle separation in curved microchannels.

Size dependent cell sorting systems and characteristic of cell groups

MHS2013 ◽  
2013 ◽  
Author(s):  
Akiyuki Hasegawa ◽  
Masumi Yamada ◽  
Minoru Seki ◽  
Masayuki Yamato ◽  
Teruo Okano
Keyword(s):  
Cell Sorting ◽  
Size Dependent ◽  
Cell Groups ◽  
Dependent Cell

scholarly journals Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Soo-Yeon Cho ◽  
Xun Gong ◽  
Volodymyr B. Koman ◽  
Matthias Kuehne ◽  
Sun Jin Moon ◽  
...  
Keyword(s):  
Near Infrared ◽  
Single Cells ◽  
Microfluidic Channel ◽  
Human Monocyte ◽  
Cellular Heterogeneity ◽  
Label Free ◽  
Nanotube Array ◽  
Chemical Cytometry ◽  
Rich Information ◽  
Non Destructive

AbstractNanosensors have proven to be powerful tools to monitor single cells, achieving spatiotemporal precision even at molecular level. However, there has not been way of extending this approach to statistically relevant numbers of living cells. Herein, we design and fabricate nanosensor array in microfluidics that addresses this limitation, creating a Nanosensor Chemical Cytometry (NCC). nIR fluorescent carbon nanotube array is integrated along microfluidic channel through which flowing cells is guided. We can utilize the flowing cell itself as highly informative Gaussian lenses projecting nIR profiles and extract rich information. This unique biophotonic waveguide allows for quantified cross-correlation of biomolecular information with various physical properties and creates label-free chemical cytometer for cellular heterogeneity measurement. As an example, the NCC can profile the immune heterogeneities of human monocyte populations at attomolar sensitivity in completely non-destructive and real-time manner with rate of ~600 cells/hr, highest range demonstrated to date for state-of-the-art chemical cytometry.

A ‘smart’ aptamer-functionalized continuous label-free cell catch-transport-release system

2021 ◽  
Author(s):  
Bozhen Zhang ◽  
Canran Wang ◽  
Yingjie Du ◽  
Rebecca Paxton ◽  
Ximin He
Keyword(s):  
Biomedical Research ◽  
Cell Sorting ◽  
Free Cell ◽  
Label Free ◽  
Clinical Therapeutics ◽  
Release System ◽  
System P

Label-free cell sorting devices are of great significance for biomedical research and clinical therapeutics. However, current platforms for label-free cell sorting cannot achieve continuity and selectivity simultaneously, resulting in complex...

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