A ‘smart’ aptamer-functionalized continuous label-free cell catch-transport-release system

2021 ◽  
Author(s):  
Bozhen Zhang ◽  
Canran Wang ◽  
Yingjie Du ◽  
Rebecca Paxton ◽  
Ximin He
Keyword(s):  
Biomedical Research ◽  
Cell Sorting ◽  
Free Cell ◽  
Label Free ◽  
Clinical Therapeutics ◽  
Release System ◽  
System P

Label-free cell sorting devices are of great significance for biomedical research and clinical therapeutics. However, current platforms for label-free cell sorting cannot achieve continuity and selectivity simultaneously, resulting in complex...

The potential of autofluorescence for the detection of single living cells for label-free cell sorting in microfluidic systems

2004 ◽  
Vol 25 (21-22) ◽  
pp. 3740-3745 ◽  
Author(s):  
Jurjen Emmelkamp ◽  
Floor Wolbers ◽  
Helene Andersson ◽  
Ralph S. DaCosta ◽  
Brian C. Wilson ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Free Cell ◽  
Living Cells ◽  
Label Free ◽  
Microfluidic Systems ◽  
Single Living Cells ◽  
Single Living

Cell detachment and label-free cell sorting using modulated surface acoustic waves (SAWs) in droplet-based microfluidics

Lab on a Chip ◽  
2014 ◽  
Vol 14 (18) ◽  
pp. 3556 ◽  
Author(s):  
Adrien Bussonnière ◽  
Yannick Miron ◽  
Michaël Baudoin ◽  
Olivier Bou Matar ◽  
Michel Grandbois ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Acoustic Waves ◽  
Surface Acoustic Waves ◽  
Free Cell ◽  
Cell Detachment ◽  
Label Free

In-droplet cell separation based on bipolar dielectrophoretic response to facilitate cellular droplet assays

Lab on a Chip ◽  
2020 ◽  
Vol 20 (20) ◽  
pp. 3832-3841 ◽  
Author(s):  
Song-I Han ◽  
Can Huang ◽  
Arum Han
Keyword(s):  
Cell Separation ◽  
Cell Types ◽  
Free Cell ◽  
Label Free ◽  
Separation Technology ◽  
Cellular Assays ◽  
System P ◽  
Distinct Cell

Novel in-droplet label-free cell separation technology is presented in this paper by utilizing different dielectrophoretic responses of two distinct cell types, enabling broader ranges of cellular assays to be implemented in the droplet-based microfluidics system.

scholarly journals Label-free cell sorting strategies via biophysical and biochemical gradients

2019 ◽  
Vol 17 ◽  
pp. 55-63 ◽  
Author(s):  
Zhengkun Chen ◽  
Xiaohe Luo ◽  
Xin Zhao ◽  
Mo Yang ◽  
Chunyi Wen
Keyword(s):  
Cell Sorting ◽  
Free Cell ◽  
Label Free

Multi-frequency single cell electrical impedance measurement for label-free cell viability analysis

The Analyst ◽  
2021 ◽  
Author(s):  
Jianwei Zhong ◽  
Dahou Yang ◽  
Yinning Zhou ◽  
Minhui Liang ◽  
Ye Ai
Keyword(s):  
Single Cell ◽  
Cell Sorting ◽  
Electrical Impedance ◽  
Impedance Measurement ◽  
Free Cell ◽  
Label Free ◽  
Cell Level ◽  
Viability Analysis ◽  
Electrical Impedance Measurement

We present a novel impedance-based strategy for high-throughput label-free single cell level viability measurement, which provides a promising pathway for real-time in situ cell identification and integration with cell sorting/isolation platforms.

scholarly journals Differential Sorting of Microparticles Using Spiral Microchannels with Elliptic Configurations

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 412
Author(s):  
Kaan Erdem ◽  
Vahid Ebrahimpour Ahmadi ◽  
Ali Kosar ◽  
Lütfullah Kuddusi
Keyword(s):  
Cell Sorting ◽  
Microfluidic Channel ◽  
Major Axis ◽  
Label Free ◽  
Flow Rates ◽  
Inertial Focusing ◽  
Size Dependent ◽  
Curved Channels ◽  
Aspect Ratios ◽  
Dependent Cell

Label-free, size-dependent cell-sorting applications based on inertial focusing phenomena have attracted much interest during the last decade. The separation capability heavily depends on the precision of microparticle focusing. In this study, five-loop spiral microchannels with a height of 90 µm and a width of 500 µm are introduced. Unlike their original spiral counterparts, these channels have elliptic configurations of varying initial aspect ratios, namely major axis to minor axis ratios of 3:2, 11:9, 9:11, and 2:3. Accordingly, the curvature of these configurations increases in a curvilinear manner through the channel. The effects of the alternating curvature and channel Reynolds number on the focusing of fluorescent microparticles with sizes of 10 and 20 µm in the prepared suspensions were investigated. At volumetric flow rates between 0.5 and 3.5 mL/min (allowing separation), each channel was tested to collect samples at the designated outlets. Then, these samples were analyzed by counting the particles. These curved channels were capable of separating 20 and 10 µm particles with total yields up to approximately 95% and 90%, respectively. The results exhibited that the level of enrichment and the focusing behavior of the proposed configurations are promising compared to the existing microfluidic channel configurations.

Label-free cell phenotypic study of opioid receptors and discovery of novel mu opioid ligands from natural products

2021 ◽  
Vol 270 ◽  
pp. 113872
Author(s):  
Tao Hou ◽  
Fangfang Xu ◽  
Xingrong Peng ◽  
Han Zhou ◽  
Xiuli Zhang ◽  
...  
Keyword(s):  
Natural Products ◽  
Opioid Receptors ◽  
Free Cell ◽  
Label Free ◽  
Mu Opioid ◽  
Opioid Ligands

Raman spectroscopy-based label-free cell identification using wavelet transform and support vector machine

RSC Advances ◽  
2016 ◽  
Vol 6 (55) ◽  
pp. 50027-50033 ◽  
Author(s):  
S. Bakhtiaridoost ◽  
H. Habibiyan ◽  
S. Muhammadnejad ◽  
M. Haddadi ◽  
H. Ghafoorifard ◽  
...  
Keyword(s):  
Support Vector Machine ◽  
Raman Spectroscopy ◽  
Wavelet Transform ◽  
Raman Spectra ◽  
Free Cell ◽  
Support Vector ◽  
Label Free ◽  
Cell Identification ◽  
Rare Cells

Wavelet transform and SVM applied to Raman spectra makes a powerful and accurate tool for identification of rare cells such as CTCs.

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