scholarly journals 3D Printing Breast Tissue Models: A Review of Past Work and Directions for Future Work

Micromachines ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 501 ◽  
Author(s):  
Chantell Cleversey ◽  
Meghan Robinson ◽  
Stephanie M. Willerth
Keyword(s):  
Tissue Engineering ◽  
Breast Tissue ◽  
In Vitro Models ◽  
Attractive Potential ◽  
Biomaterial Scaffolds ◽  
Potential Alternative ◽  
Future Work ◽  
Tissue Models

Breast cancer often results in the removal of the breast, creating a need for replacement tissue. Tissue engineering offers the promise of generating such replacements by combining cells with biomaterial scaffolds and serves as an attractive potential alternative to current surgical repair methods. Such engineered tissues can also serve as important tools for drug screening and provide in vitro models for analysis. 3D bioprinting serves as an exciting technology with significant implications and applications in the field of tissue engineering. Here we review the work that has been undertaken in hopes of generating the recognized in-demand replacement breast tissue using different types of bioprinting. We then offer suggestions for future work needed to advance this field for both in vitro and in vivo applications.

scholarly journals The Role of Biomimetic Hypoxia on Cancer Cell Behaviour in 3D Models: A Systematic Review

Cancers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1334
Author(s):  
Ye Liu ◽  
Zahra Mohri ◽  
Wissal Alsheikh ◽  
Umber Cheema
Keyword(s):  
Systematic Review ◽  
Cellular Response ◽  
3D Culture ◽  
3D Models ◽  
In Vitro Models ◽  
Research Findings ◽  
Tissue Models

The development of biomimetic, human tissue models is recognized as being an important step for transitioning in vitro research findings to the native in vivo response. Oftentimes, 2D models lack the necessary complexity to truly recapitulate cellular responses. The introduction of physiological features into 3D models informs us of how each component feature alters specific cellular response. We conducted a systematic review of research papers where the focus was the introduction of key biomimetic features into in vitro models of cancer, including 3D culture and hypoxia. We analysed outcomes from these and compiled our findings into distinct groupings to ascertain which biomimetic parameters correlated with specific responses. We found a number of biomimetic features which primed cancer cells to respond in a manner which matched in vivo response.

scholarly journals Bioreactors as physiologicallike in vitro models

2020 ◽  
Author(s):  
Sara Mantero ◽  
Federica Boschetti
Keyword(s):  
Culture Conditions ◽  
In Vitro Models ◽  
In Vivo Models ◽  
In Vitro Development ◽  
Culture Cells ◽  
Vitro Development ◽  
Tissue Models ◽  
Mechanical Stretching

Bioreactors are powerful tools for in vitro development of engineered substitutes through controlled biological, physical, and mechanical culture conditions: bioreactor technology allows a closer in vitro replication of native tissues. One of bioreactors applications is the design of in vitro 3D tissue models as a bridge between 2D and in vivo models, allowing the application of 3R (replacement, reduction, refinement) principle. To this aim, bioreactors can be used to culture cells seeded on engineered scaffolds under in vivo-like conditions. Another key use of bioreactors is for perfusion decellularization of tissues and organs to be used as scaffolds. This contribution describes a dynamic stretching. bioreactor, imposing a mechanical stretching to the cultured constructs, allowing the development of skeletal muscle engineered constructs, and a decellularization bioreactor, designed for decellularization of blood vessels.

scholarly journals Hydrogels for Liver Tissue Engineering

2019 ◽  
Vol 6 (3) ◽  
pp. 59 ◽  
Author(s):  
Shicheng Ye ◽  
Jochem W.B. Boeter ◽  
Louis C. Penning ◽  
Bart Spee ◽  
Kerstin Schneeberger
Keyword(s):  
Tissue Engineering ◽  
Liver Tissue ◽  
Drug Testing ◽  
In Vitro Models ◽  
Liver Tissue Engineering ◽  
Synthetic Materials ◽  
Toxicological Studies ◽  
End Stage

Bioengineered livers are promising in vitro models for drug testing, toxicological studies, and as disease models, and might in the future be an alternative for donor organs to treat end-stage liver diseases. Liver tissue engineering (LTE) aims to construct liver models that are physiologically relevant. To make bioengineered livers, the two most important ingredients are hepatic cells and supportive materials such as hydrogels. In the past decades, dozens of hydrogels have been developed to act as supportive materials, and some have been used for in vitro models and formed functional liver constructs. However, currently none of the used hydrogels are suitable for in vivo transplantation. Here, the histology of the human liver and its relationship with LTE is introduced. After that, significant characteristics of hydrogels are described focusing on LTE. Then, both natural and synthetic materials utilized in hydrogels for LTE are reviewed individually. Finally, a conclusion is drawn on a comparison of the different hydrogels and their characteristics and ideal hydrogels are proposed to promote LTE.

scholarly journals Smart Bioinks for the Printing of Human Tissue Models

Biomolecules ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 141
Author(s):  
Zeina Maan ◽  
Nadia Z. Masri ◽  
Stephanie M. Willerth
Keyword(s):  
Tissue Engineering ◽  
Controlled Release ◽  
Human Tissues ◽  
3D Bioprinting ◽  
Edge Field ◽  
Multiple Materials ◽  
Diseased State ◽  
Future Work ◽  
Tissue Models

3D bioprinting has tremendous potential to revolutionize the field of regenerative medicine by automating the process of tissue engineering. A significant number of new and advanced bioprinting technologies have been developed in recent years, enabling the generation of increasingly accurate models of human tissues both in the healthy and diseased state. Accordingly, this technology has generated a demand for smart bioinks that can enable the rapid and efficient generation of human bioprinted tissues that accurately recapitulate the properties of the same tissue found in vivo. Here, we define smart bioinks as those that provide controlled release of factors in response to stimuli or combine multiple materials to yield novel properties for the bioprinting of human tissues. This perspective piece reviews the existing literature and examines the potential for the incorporation of micro and nanotechnologies into bioinks to enhance their properties. It also discusses avenues for future work in this cutting-edge field.

scholarly journals Small-molecule antibiotic inhibitors of post-translational protein secretion

2020 ◽  
Author(s):  
Mohamed Belal Hamed ◽  
Ewa Burchacka ◽  
Liselotte Angus ◽  
Arnaud Marchand ◽  
Jozefien De Geyter ◽  
...  
Keyword(s):  
Small Molecule ◽  
Bacterial Resistance ◽  
Bacterial Species ◽  
Attractive Potential ◽  
E Coli ◽  
Small Molecule Compound ◽  
Potential Alternative ◽  
Structural Classes

AbstractThe increasing problem of bacterial resistance to antibiotics underscores the urgent need for new antibacterials. The Sec preprotein export pathway is an attractive potential alternative target. It is essential for bacterial viability and includes components that are absent from eukaryotes. Here we used a new high throughput in vivo screen based on the secretion and activity of alkaline phosphatase (PhoA), a Sec-dependent secreted enzyme that becomes active in the periplasm. The assay was optimized for a luminescence-based substrate and was used to screen a ~240K small molecule compound library. After hit confirmation and analoging, fourteen HTS secretion inhibitors (HSI), belonging to 8 structural classes, were identified (IC50 <60 μM). The inhibitors were also evaluated as antibacterials against 19 Gram− and Gram+ bacterial species (including those from the WHO top pathogens list). Seven of them, HSI#6, 9; HSI#1, 5, 10 and HSI#12, 14 representing three structural families were microbicidals. HSI#6 was the most potent (IC50 of 0.4-8.7 μM), against 13 species of both Gram− and Gram+ bacteria. HSI#1, 5, 9 and 10 inhibited viability of Gram+ bacteria with IC50 ~6.9-77.8 μM. HSI#9, 12 and 14 inhibited viability of E. coli strains with IC50 <65 μM. Moreover, HSI#1, 5 and 10 inhibited viability of an E. coli strain missing TolC to improve permeability with IC50 4-14 μM, indicating their inability to penetrate the outer membrane. In vitro assays revealed that antimicrobial activity was not related to inhibition of the SecA component of the translocase and hence HSI molecules may target new unknown components that affect secretion. The results provide proof of principle for our approach, and new starting compounds for optimization.

scholarly journals On the transferability of tissue engineering technologies to the design of tissue models

2020 ◽  
Author(s):  
Gianluca Ciardelli
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Molecular Mechanisms ◽  
Disease Onset ◽  
In Vitro Models ◽  
Ageing Process ◽  
Tissue Models ◽  
Insight Into

3D tissue-engineered models are promising tools in the screening and evaluation of drugs and therapies as well as in the investigation of the molecular mechanisms involved in disease onset and progression. In this context, we describe our efforts in soft tissue replication, to design in vitro models that have the potential to provide better insight into the development of ageing process and related pathologies, with particular reference to the cardiovascular field.

scholarly journals 3D bioprinting technology for regenerative medicine applications

2016 ◽  
Vol 2 (2) ◽  
Author(s):  
Dhakshinamoorthy Sundaramurthi ◽  
Sakandar Rauf ◽  
Charlotte Hauser
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Drug Testing ◽  
Severe Disease ◽  
Three Dimensional ◽  
In Vitro Models ◽  
3D Bioprinting ◽  
Organ Printing ◽  
Tissue Models

Alternative strategies that overcome existing organ transplantation methods are of increasing importance be-cause of ongoing demands and lack of adequate organ donors. Recent improvements in tissue engineering techniques offer improved solutions to this problem and will influence engineering and medicinal applications. Tissue engineering employs the synergy of cells, growth factors and scaffolds besides others with the aim to mimic the native extracellular matrix for tissue regeneration. Three-dimensional (3D) bioprinting has been explored to create organs for transplanta-tion, medical implants, prosthetics, in vitro models and 3D tissue models for drug testing. In addition, it is emerging as a powerful technology to provide patients with severe disease conditions with personalized treatments. Challenges in tis-sue engineering include the development of 3D scaffolds that closely resemble native tissues. In this review, existing printing methods such as extrusion-based, robotic dispensing, cellular inkjet, laser-assisted printing and integrated tissue organ printing (ITOP) are examined. Also, natural and synthetic polymers and their blends as well as peptides that are exploited as bioinks are discussed with emphasis on regenerative medicine applications. Furthermore, applications of 3D bioprinting in regenerative medicine, evolving strategies and future perspectives are summarized.

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

In vitro- / in vivo- Untersuchungen zur Biokompatibilität und Bioresorption amorpher Kieselgelfaser für das Tissue engineering humaner Knorpelgewebe

2004 ◽  
Vol 83 (02) ◽  
Author(s):  
A Haisch ◽  
A Evers ◽  
K Jöhrens-Leder ◽  
S Jovanovic ◽  
B Sedlmaier ◽  
...  
Keyword(s):  
Tissue Engineering

Surface Modification by Nanobiomaterials for Vascular Tissue Engineering Applications

2020 ◽  
Vol 27 (10) ◽  
pp. 1634-1646 ◽  
Author(s):  
Huey-Shan Hung ◽  
Shan-hui Hsu
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Thrombus Formation ◽  
Vascular Grafts ◽  
Vascular Tissue Engineering ◽  
Great Success ◽  
Natural Polymers ◽  
Vascular Biomaterials

Treatment of cardiovascular disease has achieved great success using artificial implants, particularly synthetic-polymer made grafts. However, thrombus formation and restenosis are the current clinical problems need to be conquered. New biomaterials, modifying the surface of synthetic vascular grafts, have been created to improve long-term patency for the better hemocompatibility. The vascular biomaterials can be fabricated from synthetic or natural polymers for vascular tissue engineering. Stem cells can be seeded by different techniques into tissue-engineered vascular grafts in vitro and implanted in vivo to repair the vascular tissues. To overcome the thrombogenesis and promote the endothelialization effect, vascular biomaterials employing nanotopography are more bio-mimic to the native tissue made and have been engineered by various approaches such as prepared as a simple surface coating on the vascular biomaterials. It has now become an important and interesting field to find novel approaches to better endothelization of vascular biomaterials. In this article, we focus to review the techniques with better potential improving endothelization and summarize for vascular biomaterial application. This review article will enable the development of biomaterials with a high degree of originality, innovative research on novel techniques for surface fabrication for vascular biomaterials application.

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