scholarly journals Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells

Marine Drugs ◽  
2009 ◽  
Vol 7 (4) ◽  
pp. 589-599 ◽  
Author(s):  
Taiko Oda ◽  
Jong-Soo Lee ◽  
Yuta Sato ◽  
Yasuaki Kabe ◽  
Satoshi Sakamoto ◽  
...  
Keyword(s):  
Inflammatory Cytokine ◽  
Cytokine Production ◽  
Inhibitory Effect ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Inflammatory Cytokine Production

scholarly journals Neutral Polysaccharide from the Leaves of Pseuderanthemum carruthersii: Presence of 3-O-Methyl Galactose and Anti-Inflammatory Activity in LPS-Stimulated RAW 264.7 Cells

Polymers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1219 ◽  
Author(s):  
Vo Hoai Bac ◽  
Berit Smestad Paulsen ◽  
Le Van Truong ◽  
Andreas Koschella ◽  
Tat Cuong Trinh ◽  
...  
Keyword(s):  
Inflammatory Cytokine ◽  
Cytokine Production ◽  
Water Extract ◽  
Mapk Signaling ◽  
Raw 264.7 Cells ◽  
Oxygen Species ◽  
Inflammatory Cytokine Production ◽  
Mouse Macrophages ◽  
Chemotaxonomic Marker

Pseuderanthemum carruthersii (Seem.) Guillaumin is a native tree in Vietnam. The water extract of the leaves from this tree gives a highly viscous product that has been used to heal wounds and treat inflammations. Our previous studies showed that the leaves of P. carruthersii have a high content of polysaccharides. In this study, the structure and influence of the neutral polysaccharide from Pseuderanthemum carruthersii (PCA1) on lipopolysaccharide (LPS)-stimulated RAW264.7 cells were investigated. The PCA1 isolated from P. carruthersii is a galactan-type polysaccharide, containing galactose (77.0%), 3-O-methyl galactose (20.0%), and arabinose (3.0%). Linkage analysis of PCA1 showed that both the 3-O-methyl galactose and galactose were 1,4-linked. The presence of 3-O-methyl galactose units as part of the polysaccharide is important and can be used as a chemotaxonomic marker. The molecular weight of the PCA1 was 170 kDa. A PCA1 concentration of 30–40 μg/mL strongly inhibited TNFα, IL-1β, and IL-6 inflammatory cytokine production, and reactive oxygen species (ROS) release. PCA1 had inhibitory activities on pro-inflammatory cytokine and ROS release in LPS-stimulated mouse macrophages in vitro through MAPK signaling.

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