scholarly journals Promoter Trapping in Microalgae Using the Antibiotic Paromomycin as Selective Agent

Marine Drugs ◽  
2012 ◽  
Vol 10 (12) ◽  
pp. 2749-2765 ◽  
Author(s):  
Marta Vila ◽  
Encarnación Díaz-Santos ◽  
Marta de la Vega ◽  
Herminia Rodríguez ◽  
Ángeles Vargas ◽  
...  
Keyword(s):  
Selective Agent ◽  
Promoter Trapping

scholarly journals Screening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
I. A. H. Khwajah Mohinudeen ◽  
Saumya Pandey ◽  
Hemalatha Kanniyappan ◽  
Vignesh Muthuvijayan ◽  
Smita Srivastava
Keyword(s):  
Natural Habitat ◽  
Product Yield ◽  
Study Strategies ◽  
Selective Agent ◽  
Nothapodytes Nimmoniana ◽  
Time Consumption ◽  
Potential Alternative ◽  
Plant Metabolite ◽  
Produce Plant ◽  
Selection Of

AbstractEndophytic fungi with the ability to produce plant based secondary metabolites are a potential alternative for producing the host plant metabolite and to prevent natural plants from extinction. To isolate a high metabolite yielding endophytic strain from plants, hundreds of endophytic strains are screened and tested for product yield separately under axenic state, before shortlisting the potential endophyte, which involves huge time consumption. In this study, strategies for screening and selection of high camptothecin yielding endophytes from their natural habitat were proposed. A correlation was built between the camptothecin yield in the explants and the endophytes isolated from them. In addition, camptothecin yield was compared between the endophytes isolated from young and matured plants. Further, camptothecin producers and non-producers strains were compared for their tolerance toward camptothecin. The study indicates that high camptothecin yielding endophytes were isolated from high yielding explants and younger plants and they were more tolerant to camptothecin in comparison to non-camptothecin yielding endophytes. Thus, choosing a young and high yielding explant for endophyte isolation, and use of camptothecin as a selective agent in the growth medium, can be instrumental in screening and selection of high camptothecin yielding endophytes from nature in relatively less time.

Use of paromomycin as a selective agent for oat transformation

1995 ◽  
Vol 14 (10) ◽  
pp. 635-640 ◽  
Author(s):  
Kimberly A. Torbert ◽  
Howard W. Rines ◽  
David A. Somers
Keyword(s):  
Selective Agent

U50,488: A kappa-selective agent with poor affinity for mu1 opiate binding sites

1988 ◽  
Vol 27 (3) ◽  
pp. 331-332 ◽  
Author(s):  
Janet A. Clark ◽  
G.W. Pasternak
Keyword(s):  
Binding Sites ◽  
Selective Agent ◽  
Opiate Binding

scholarly journals Promoter trapping in Lotus japonicus reveals novel root and nodule GUS expression domains

2005 ◽  
Vol 46 (8) ◽  
pp. 1202-1212 ◽  
Author(s):  
Diana Mihaela Buzas ◽  
Dasharath Lohar ◽  
Shusei Sato ◽  
Yasukazu Nakamura ◽  
Satoshi Tabata ◽  
...  
Keyword(s):  
Lotus Japonicus ◽  
Gus Expression ◽  
Promoter Trapping

Study of Red Halophilic Bacteria in Solar Salt and Salted Fish: II. Bacto-oxgall as a Selective Agent for Differentiation

1956 ◽  
Vol 13 (2) ◽  
pp. 195-199 ◽  
Author(s):  
H. P. Dussault
Keyword(s):  
Halophilic Bacteria ◽  
Partial Identification ◽  
Selective Medium ◽  
Selective Agent ◽  
Simple Method ◽  
Solar Salt ◽  
Salted Fish ◽  
Low Concentrations ◽  
Fish Samples

A simple method, based on oxgall tolerance, is proposed for differentiating red halophilic bacteria commonly found in solar salt and discoloured salted codfish. Tests carried out on 18 strains isolated from various sources have shown that the rod forms are inhibited by low concentrations of Bacto-oxgall and that the coccus forms tolerate relatively high ones. Bacto-oxgall can thus be used as the basis of a simple selective medium. This test has been found useful for the isolation, purification and partial identification of unidentified strains and also for determining the relative proportions of the two main types of red halophilic bacteria present in solar salt and salted fish samples.

scholarly journals 2-Deoxyglucose as a Selective Agent for Derepressed Mutants of Pichia stipitis

1999 ◽  
Vol 77 (1-3) ◽  
pp. 211-222 ◽  
Author(s):  
Hassan K. Sreenath ◽  
Thomas W. Jeffries
Keyword(s):  
Pichia Stipitis ◽  
Selective Agent

Sampling bias created by ampicillin in isolation media forAeromonas

2007 ◽  
Vol 53 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Jennifer R Huddleston ◽  
John C Zak ◽  
Randall M Jeter
Keyword(s):  
Sampling Bias ◽  
Aeromonas Species ◽  
Aquatic Environments ◽  
Selective Agent ◽  
Opportunistic Pathogens ◽  
Chi Square ◽  
Isolation Medium ◽  
Ability To Act ◽  
Chi Square Analysis ◽  
The Impact

Members of the bacterial genus Aeromonas are widely isolated from aquatic environments and studied in part for their ability to act as opportunistic pathogens in a variety of animals. All aeromonads, with the exception of Aeromonas trota, are generally thought to be resistant to ampicillin, so the antibiotic is frequently added to isolation medium as a selective agent. In this study, 282 aeromonads from environmental sources were isolated on a medium without ampicillin and their resistance to ampicillin determined. Of the 104 of these isolates that were judged to be independent (nonredundant), 18 (17.3%) were susceptible to ampicillin. A chi-square analysis was performed to determine the impact of ampicillin use on enumerating Aeromonas species from environmental samples. Our results indicate that, when ampicillin is used as a selective agent, a significant portion of the aeromonad population in at least some environ ments can be omitted from isolation.Key words: Aeromonas, ampicillin, selective media.

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