scholarly journals 2-Deoxyglucose as a Selective Agent for Derepressed Mutants of Pichia stipitis

1999 ◽  
Vol 77 (1-3) ◽  
pp. 211-222 ◽  
Author(s):  
Hassan K. Sreenath ◽  
Thomas W. Jeffries
Keyword(s):  
Pichia Stipitis ◽  
Selective Agent

Structural and mechanistic insight into stem‐loop RNA processing by yeast Pichia stipitis Dicer

2021 ◽  
Author(s):  
Chan Jing Ru ◽  
Fu Qinqin ◽  
Li Jianwei ◽  
Chen Ying ◽  
Satoru Machida ◽  
...  
Keyword(s):  
Rna Processing ◽  
Pichia Stipitis ◽  
Stem Loop ◽  
Mechanistic Insight ◽  
Insight Into

scholarly journals Screening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
I. A. H. Khwajah Mohinudeen ◽  
Saumya Pandey ◽  
Hemalatha Kanniyappan ◽  
Vignesh Muthuvijayan ◽  
Smita Srivastava
Keyword(s):  
Natural Habitat ◽  
Product Yield ◽  
Study Strategies ◽  
Selective Agent ◽  
Nothapodytes Nimmoniana ◽  
Time Consumption ◽  
Potential Alternative ◽  
Plant Metabolite ◽  
Produce Plant ◽  
Selection Of

AbstractEndophytic fungi with the ability to produce plant based secondary metabolites are a potential alternative for producing the host plant metabolite and to prevent natural plants from extinction. To isolate a high metabolite yielding endophytic strain from plants, hundreds of endophytic strains are screened and tested for product yield separately under axenic state, before shortlisting the potential endophyte, which involves huge time consumption. In this study, strategies for screening and selection of high camptothecin yielding endophytes from their natural habitat were proposed. A correlation was built between the camptothecin yield in the explants and the endophytes isolated from them. In addition, camptothecin yield was compared between the endophytes isolated from young and matured plants. Further, camptothecin producers and non-producers strains were compared for their tolerance toward camptothecin. The study indicates that high camptothecin yielding endophytes were isolated from high yielding explants and younger plants and they were more tolerant to camptothecin in comparison to non-camptothecin yielding endophytes. Thus, choosing a young and high yielding explant for endophyte isolation, and use of camptothecin as a selective agent in the growth medium, can be instrumental in screening and selection of high camptothecin yielding endophytes from nature in relatively less time.

Ethanol Production from Corn Stover Using Soaking Pretreatment

2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Author(s):  
Zhuang Zuo ◽  
Xiu Shan Yang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Liquid Ratio ◽  
Mild Conditions ◽  
Whole Process ◽  
Conversion Rates ◽  
Mild Temperature ◽  
Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

Use of paromomycin as a selective agent for oat transformation

1995 ◽  
Vol 14 (10) ◽  
pp. 635-640 ◽  
Author(s):  
Kimberly A. Torbert ◽  
Howard W. Rines ◽  
David A. Somers
Keyword(s):  
Selective Agent

U50,488: A kappa-selective agent with poor affinity for mu1 opiate binding sites

1988 ◽  
Vol 27 (3) ◽  
pp. 331-332 ◽  
Author(s):  
Janet A. Clark ◽  
G.W. Pasternak
Keyword(s):  
Binding Sites ◽  
Selective Agent ◽  
Opiate Binding

Study of Red Halophilic Bacteria in Solar Salt and Salted Fish: II. Bacto-oxgall as a Selective Agent for Differentiation

1956 ◽  
Vol 13 (2) ◽  
pp. 195-199 ◽  
Author(s):  
H. P. Dussault
Keyword(s):  
Halophilic Bacteria ◽  
Partial Identification ◽  
Selective Medium ◽  
Selective Agent ◽  
Simple Method ◽  
Solar Salt ◽  
Salted Fish ◽  
Low Concentrations ◽  
Fish Samples

A simple method, based on oxgall tolerance, is proposed for differentiating red halophilic bacteria commonly found in solar salt and discoloured salted codfish. Tests carried out on 18 strains isolated from various sources have shown that the rod forms are inhibited by low concentrations of Bacto-oxgall and that the coccus forms tolerate relatively high ones. Bacto-oxgall can thus be used as the basis of a simple selective medium. This test has been found useful for the isolation, purification and partial identification of unidentified strains and also for determining the relative proportions of the two main types of red halophilic bacteria present in solar salt and salted fish samples.

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