scholarly journals Impact of Polyethylene Glycol Functionalization of Graphene Oxide on Anticoagulation and Haemolytic Properties of Human Blood

Materials ◽  
2021 ◽  
Vol 14 (17) ◽  
pp. 4853
Author(s):  
Trayana Kamenska ◽  
Miroslav Abrashev ◽  
Milena Georgieva ◽  
Natalia Krasteva
Keyword(s):  
Graphene Oxide ◽  
Polyethylene Glycol ◽  
Human Blood ◽  
Blood Cells ◽  
Biomedical Applications ◽  
Molecular Design ◽  
Coagulation Factors ◽  
Oxide Nanoparticles ◽  
Contrast Imaging ◽  
Blood Coagulation Factors

Graphene oxide (GO) is one of the most explored nanomaterials in recent years. It has numerous biomedical applications as a nanomaterial including drug and gene delivery, contrast imaging, cancer treatment, etc. Since most of these applications need intravenous administration of graphene oxide and derivatives, the evaluation of their haemocompatibility is an essential preliminary step for any of the developed GO applications. Plentiful data show that functionalization of graphene oxide nanoparticles with polyethylene glycol (PEG) increases biocompatibility, thus allowing PEGylated GO to elicit less dramatic blood cell responses than their pristine counterparts. Therefore, in this work, we PEGylated graphene oxide nanoparticles and evaluated the effects of their PEGylation on the structure and function of human blood components, especially on the morphology and the haemolytic potential of red blood cells (RBCs). Further, we studied the effect of PEGylation on some blood coagulation factors, including plasma fibrinogen as well as on the activated partial thromboplastin (aPTT), prothrombin time (PT) and platelet aggregation. Our findings provide important information on the mechanisms through which PEGylation increases GO compatibility with human blood cells. These data are crucial for the molecular design and biomedical applications of PEGylated graphene oxide nanomaterials in the future.

High-level production of human blood coagulation factors VII and XI using a new mammalian expression vector

Gene ◽  
1994 ◽  
Vol 139 (2) ◽  
pp. 275-279 ◽  
Author(s):  
Geoffrey Kemball-Cook ◽  
Ian Garner ◽  
Yasufumi Imanaka ◽  
Takuya Nishimura ◽  
Donogh P. O'Brien ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Human Blood ◽  
Expression Vector ◽  
Coagulation Factors ◽  
Blood Coagulation Factors ◽  
Mammalian Expression ◽  
Mammalian Expression Vector ◽  
High Level ◽  
Level Production ◽  
Human Blood Coagulation

Iron Oxide Nanoparticles Are Less Toxic to Endothelial Cells When Coated With Dextran and Polyethylene Glycol

2011 ◽  
Author(s):  
Miao Yu ◽  
Vladimir Muzykantov ◽  
Alisa Morss Clyne
Keyword(s):  
Polyethylene Glycol ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cell Mechanics ◽  
Biomedical Applications ◽  
Oxide Nanoparticles ◽  
Atherosclerotic Plaques ◽  
Oxygen Species ◽  
Cell Functions

Iron oxide nanoparticles are of particular interest for drug delivery applications, since they can be targeted to a specific location using a magnetic field. We are interested in delivering drugs to atherosclerotic plaques via these nanoparticles. However, prior to using nanoparticles in vivo, they must be shown as relatively non-toxic to cells. We and others have shown that bare iron oxide nanoparticles are readily taken up by cells, where they catalyze production of highly toxic reactive oxygen species [1]. This oxidative stress disrupts the cell cytoskeleton, alters cell mechanics, and may change other critical cell functions. Iron oxide nanoparticles for in vivo biomedical applications are often coated with a polysaccharide (eg. dextran) or a polymer (eg. polyethylene glycol, PEG). Both the size and the surface coating of the nanoparticle play an important role in cell toxicity.

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