scholarly journals Ferroferric oxide nanoparticles induce prosurvival autophagy in human blood cells by modulating the Beclin 1/Bcl-2/VPS34 complex

2014 ◽  
pp. 207 ◽  
Author(s):  
Xinliang Mao ◽  
Min Shi ◽  
Liang Cheng ◽  
Zubin Zhang ◽  
Zhuang Liu
Keyword(s):  
Human Blood ◽  
Blood Cells ◽  
Beclin 1 ◽  
Oxide Nanoparticles ◽  
Ferroferric Oxide ◽  
Human Blood Cells

scholarly journals Cytotoxicity and genotoxicity of iron oxide nanoparticles: An in vitro biosafety study

2016 ◽  
Vol 68 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Erdal Sonmez ◽  
Elanur Aydin ◽  
Hasan Turkez ◽  
Elvan Özbek ◽  
Basak Togar ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Oxidative Damage ◽  
Cell Viability ◽  
Iron Oxide Nanoparticles ◽  
Human Blood ◽  
Blood Cells ◽  
Biological Effects ◽  
Oxide Nanoparticles ◽  
Human Blood Cells ◽  
Dose Dependent

With the development of nanotechnology and the wide use of iron oxide nanoparticles, it has become necessary to assess the potential adverse biological effects of magnetite. This study investigated the cytotoxicity, genotoxicity and oxidative damage of different concentrations of magnetite (0 to 1000 mg/L) in human whole blood cultures. After supplementation of magnetite, the blood samples were incubated for 72 h. Cell viability was assessed by the 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays. The total antioxidant capacity (TAC) and total oxidant status (TOS) were determined to evaluate the dose-dependent effects of magnetite on the oxidant/antioxidant balance and to evaluate the potential oxidative injury due to increased oxidative stress. Genotoxicity was estimated by by the sister chromatid exchange (SCE), micronuclei (MN) and chromosome aberration (CA) assays and determination of 8-oxo-2-deoxyguanosine (8-OH-dG) levels. The results of MTT and LDH assays showed that the higher concentrations of magnetite (100, 150, 300, 500 and 1000 mg/L) decreased cell viability. Concentrations of magnetite higher than 10 mg/L increased TOS levels and decreased TAC levels in human blood cells. Increasing concentrations of magnetite caused significant increases in MN, SCE and CA rates and 8-OH-dG levels. The obtained results showed that magnetite exerted dose-dependent effects on oxidative damage, genotoxicity and cytotoxicity in human blood cells.

Resonance impedance sensing of human blood cells

2008 ◽  
Vol 145-146 ◽  
pp. 29-36 ◽  
Author(s):  
Siyang Zheng ◽  
Mandheerej S. Nandra ◽  
Chi-Yuan Shih ◽  
Wei Li ◽  
Yu-Chong Tai
Keyword(s):  
Human Blood ◽  
Blood Cells ◽  
Impedance Sensing ◽  
Human Blood Cells

scholarly journals Differential DNA Methylation in Purified Human Blood Cells: Implications for Cell Lineage and Studies on Disease Susceptibility

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e41361 ◽  
Author(s):  
Lovisa E. Reinius ◽  
Nathalie Acevedo ◽  
Maaike Joerink ◽  
Göran Pershagen ◽  
Sven-Erik Dahlén ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Human Blood ◽  
Blood Cells ◽  
Disease Susceptibility ◽  
Cell Lineage ◽  
Human Blood Cells

Changes of anti-oxidant levels in human blood cells during early post-renal transplant period

2003 ◽  
Vol 35 (1) ◽  
pp. 255 ◽  
Author(s):  
S Arita ◽  
K Yamada ◽  
S Yoshida ◽  
K Sakamoto ◽  
C Iwashita ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Human Blood ◽  
Blood Cells ◽  
Human Blood Cells ◽  
Anti Oxidant
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