scholarly journals Settable Polymeric Autograft Extenders in a Rabbit Radius Model of Bone Formation

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 3960
Author(s):  
Lauren A. Boller ◽  
Madison A.P. McGough ◽  
Stefanie M. Shiels ◽  
Craig L. Duvall ◽  
Joseph C. Wenke ◽  
...  
Keyword(s):  
Bone Formation ◽  
Gold Standard ◽  
Bone Growth ◽  
Bone Grafts ◽  
Polymer Composition ◽  
Cellular Infiltration ◽  
New Bone Formation ◽  
Defect Model ◽  
Patient Morbidity

Autograft (AG) is the gold standard for bone grafts, but limited quantities and patient morbidity are associated with its use. AG extenders have been proposed to minimize the volume of AG while maintaining the osteoinductive properties of the implant. In this study, poly(ester urethane) (PEUR) and poly(thioketal urethane) (PTKUR) AG extenders were implanted in a 20-mm rabbit radius defect model to evaluate new bone formation and graft remodeling. Outcomes including µCT and histomorphometry were measured at 12 weeks and compared to an AG (no polymer) control. AG control examples exhibited new bone formation, but inconsistent healing was observed. The implanted AG control was resorbed by 12 weeks, while AG extenders maintained implanted AG throughout the study. Bone growth from the defect interfaces was observed in both AG extenders, but residual polymer inhibited cellular infiltration and subsequent bone formation within the center of the implant. PEUR-AG extenders degraded more rapidly than PTKUR-AG extenders. These observations demonstrated that AG extenders supported new bone formation and that polymer composition did not have an effect on overall bone formation. Furthermore, the results indicated that early cellular infiltration is necessary for harnessing the osteoinductive capabilities of AG.

scholarly journals Growth Dynamic of Allogeneic and Autogenous Bone Grafts in a Vertical Model

2018 ◽  
Vol 29 (4) ◽  
pp. 325-334 ◽  
Author(s):  
Julio Leonardo de Oliveira Lima ◽  
Daniel Isaac Sendyk ◽  
Wilson Roberto Sendyk ◽  
Cristiane Ibanhes Polo ◽  
Luciana Correa ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Growth ◽  
Pure Titanium ◽  
Bone Grafts ◽  
Autogenous Bone ◽  
Growth Dynamic ◽  
Freeze Dried ◽  
Fresh Frozen ◽  
Hollow Cylinders ◽  
Autogenous Bone Grafts

Abstract Several techniques have been proposed for vertical bone regeneration, and many of them use bone autogenous and allogeneic grafts. The purpose of this study was to compare demineralised freeze-dried bone allografts (DFDBA), fresh-frozen (FF) allografts, autogenous bone grafts to find differences between volumetric and histological quantity of bone formation and vertical bone growth dynamic. A vertical tissue regeneration bone model was performed in rabbit calvarias under general anaesthesia. Four hollow cylinders of pure titanium were screwed onto external cortical bone calvarias in eight rabbits. Each one of the cylinders was randomly filled with one intervention: DFDBA, FF, autogenous bone, or left to be filled with blood clot (BC) as control. Allogeneic grafts were obtained from a ninth animal following international standardised protocols for the harvesting, processing, and cryopreservation of allografts. Autogenous graft was obtained from the host femur scraping before adapting hollow cylinders. Animals were euthanized at 13 weeks. Vertical volume was calculated after probe device measurements of the new formed tissue inside the cylinders and after titanium cylinders were removed. Histomorphometry and fluorochrome staining were used to analyse quantity and dynamic of bone formation, respectively. Results showed that DFDBA and fresh-frozen bone improved the velocity and the quantity of bone deposition in distant portions of the basal plane of grafting. Remaining material in allograft groups was more intense than in autogenous group. Both allografts can be indicated as reliable alternatives for volume gain and vertical bone augmentation.

Quantitative Analysis of New Bone Formation in Various Bone Grafts by In - Vivo Tetracycline Labeling

1985 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Sang Cheol Seong ◽  
Young Min Kim ◽  
Han Koo Lee ◽  
In Ho Choi ◽  
Moon Sang Chung ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Bone Formation ◽  
Bone Grafts ◽  
New Bone Formation ◽  
Tetracycline Labeling

Evaluation of Gamma Irradiated Human Bone in Nude Rats

2005 ◽  
Vol 288-289 ◽  
pp. 269-272
Author(s):  
Y. Yu ◽  
Jin Biao Chen ◽  
J.L. Yang ◽  
D.A.F. Morgan ◽  
W.R. Walsh
Keyword(s):  
Bone Formation ◽  
Gamma Irradiation ◽  
Soft Tissues ◽  
Bone Grafts ◽  
Human Bone ◽  
New Bone Formation ◽  
Nude Rats ◽  
Defect Area ◽  
Post Operation ◽  
Gamma Irradiated

Deep-frozen morselized human bone grafts showed osteoconductivity and osteoinductivity when implanted into tibial window defects of nude rats. The osteoconductivity was assessed by measuring the total area of newly formed bone bridged by the implanted bone grafts in the entire defect area. The osteoinductivity was evidenced by the presence of active osteoblast-like cells and new bone formation around the implanted bone grafts, which were surrounded by soft tissues distant from the host cortex. Gamma irradiation at the doses of 15 or 25 kGray reduced the osteoconductivity (ANOVA and LSD tests, p<0.05) at 3 weeks post operation. The 25 kGray group had a significantly lower level of new bone formation compared with the 0 and 15 kGray groups. The evidences of osteoinductivity were only noted in the 0 and 15 kGray groups. Our data indicate that 25 kGray gamma irradiation reduces the osteoconductive and osteoinductive properties of the morselized human bone graft.

Comparison of Bone Substitutes in a Tibia Defect Model in Wistar-Rats

2011 ◽  
Vol 493-494 ◽  
pp. 732-738 ◽  
Author(s):  
Cornelia Ganz ◽  
W. Xu ◽  
G. Holzhüter ◽  
W. Götz ◽  
B. Vollmar ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Substitutes ◽  
Porous Matrix ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Defect Model ◽  
Inflammatory Reactions ◽  
Nanocrystalline Hydroxyapatite ◽  
Defect Area ◽  
Tibia Defect

Various bone graft substitutes were used in clinical practise in the treatment of bone defects after trauma or osteoporosis. Many synthetic biomaterials were developed in recent years primarily based on hydroxyapatite (HA). NanoBone® is a nanocrystalline hydroxyapatite (HA) embedded in a porous matrix of silica (SiO2). The ratio of HA:SiO2 varied between 76:24 (wt%; NanoBone®) and 61:39 (wt%; Nanobone® S). The two bone substitutes NB and NB S and a natural bovine bone substitute Bio-Oss® (BO) were evaluated by means of implantation in the tibia of the rat. The aim of this study was to analyze the remodelling process and to measure new bone formation and degradation after implantation of these biomaterials. A tibia defect model was used for all investigations with testing periods of 12, 21 and 84 days. (n=5 for each time point). The results showed, that all bone grafts were well accepted by the host tissue without inflammatory reactions. In comparison to the biomaterial BO, NanoBone® and NanoBone® S were quickly degraded, whereas autologous proteins were incorporated into nanopores. New bone formation was statistically higher in NanoBone® S compared to Bio-Oss® in defect area after 84 days implantation. The presence of osteoclasts in tissue sections were demonstrated by TRAP- and ED1-immunohistology.

Retracted: New bone formation in bone defects after melatonin and porcine bone grafts: experimental study in rabbits

2014 ◽  
Vol 26 (4) ◽  
pp. 399-406 ◽  
Author(s):  
José-Luis Calvo-Guirado ◽  
Gerardo Gómez-Moreno ◽  
José-Eduardo Maté-Sánchez ◽  
Laura López-Marí ◽  
Rafael Delgado-Ruiz ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bone Formation ◽  
Bone Grafts ◽  
Bone Defects ◽  
New Bone Formation

scholarly journals Tyrosine-derived polycarbonate membrane in treating mandibular bone defects. An experimental study

2006 ◽  
Vol 3 (10) ◽  
pp. 629-635 ◽  
Author(s):  
Antti J Asikainen ◽  
Jukka Noponen ◽  
Christian Lindqvist ◽  
Mika Pelto ◽  
Minna Kellomäki ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Growth ◽  
Bone Defects ◽  
Membrane Thickness ◽  
New Bone Formation ◽  
Polycarbonate Membrane ◽  
Good Biocompatibility ◽  
The Difference ◽  
Group 2 ◽  
Group 1

This study was designed to evaluate the suitability of a novel bioabsorbable material in treating bone defects. A poly(desaminotyrosyl-tyrosine-ethyl ester carbonate) (PDTE carbonate) membrane (thickness 0.2–0.3 mm) was implanted into the mandibular angle of 20 New Zealand White rabbits to cover a through-and-through defect (12×6 mm). In group 1, the defects were left unfilled but covered with membrane and in group 2 the defects were filled with bioactive glass mesh and covered with membrane, too. Controls were left uncovered and unfilled. The animals were followed for 6, 12, 24 and 52 weeks, respectively. The material was evaluated by qualitative analysis of histological reactions and newly formed bone. We found that PDTE carbonate elicited a modest foreign body reaction in the tissues, which was uniform throughout the study. New bone formation was seen in all samples after six weeks. Group 1 had more new bone formation until 24 weeks and after this the difference settled. Based on findings of this study it was concluded that PDTE carbonate membranes have good biocompatibility and are sufficient to enhance bone growth without additional supportive matrix.

The Potential of Antibiotic Collagen Based Biocomposites for the Treatment of Bone Defects

2013 ◽  
Vol 587 ◽  
pp. 404-411
Author(s):  
Ioan Cristescu ◽  
Lucian Marina ◽  
Daniel Vilcioiu ◽  
F. Safta ◽  
M. Istodorescu ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Growth ◽  
Local Treatment ◽  
Bone Destruction ◽  
Bone Defects ◽  
New Bone Formation ◽  
Collagen Scaffolds ◽  
Local Tissue ◽  
Local Bone ◽  
Antibiotic Delivery

Antibiotic delivery systems used in the past have consisted primarily of impregnated cement beads that required routine removal once the antibiotic had eluded completely. With the development of collagen scaffolds that could be used to fill bony defects the antibiotic cold be delivered from the scaffold used to sustain local bone growth. Over the course of two years antibiotic loaded collagen scaffolds were used in the local treatment of 21patients suffering of complicated fractures including bone defects, infections or pseudoarthrosis, all of them of traumatic nature. At the time of the initial surgical debridement or at subsequent second look procedures once local tissue viability was observed the antibiotic loaded collagen scaffold was inserted in the tissue defect and never removed. Excellent results were obtained and the infection was brought under control by use of both surgical and antibiotic modalities. Bone grafting was used in 6 cases where the defects were extensive. Where there was less extensive bone destruction the scaffold was a good adjuvant in new bone formation. Use of antibiotic loaded collagen scaffolds is a reliable and effective means of local antibiotic delivery system combining both the new bone formation capacity of the scaffold to hold osteoblasts with the ability to deliver high doses of antibiotic in the local tissue environment and thus avoiding the systemic toxicity.

Sign in / Sign up

Export Citation Format

Share Document