Effect of recombinant human bone morphogenic protein 9 (rhBMP9) loaded onto bone grafts versus barrier membranes on new bone formation in a rabbit calvarial defect model

2017 ◽  
Vol 105 (10) ◽  
pp. 2655-2661 ◽  
Author(s):  
Masako Fujioka-Kobayashi ◽  
Eizaburo Kobayashi ◽  
Benoit Schaller ◽  
Matthias Mottini ◽  
Richard J. Miron ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Grafts ◽  
Bone Morphogenic Protein ◽  
Human Bone ◽  
Calvarial Defect ◽  
New Bone Formation ◽  
Defect Model ◽  
Barrier Membranes ◽  
Recombinant Human Bone

scholarly journals Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model

Materials ◽  
2019 ◽  
Vol 12 (10) ◽  
pp. 1613
Author(s):  
Eunhye Jang ◽  
Ja-Youn Lee ◽  
Eun-Young Lee ◽  
Hyun Seok
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Human Bone ◽  
Control Group ◽  
New Bone Formation ◽  
Morphogenetic Protein ◽  
Regeneration Effect ◽  
Recombinant Human Bone

The aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP) (5 µg), and ABB/high BMP (50 µg) were grafted as subperiosteal bone grafts. The new bone formation parameters of bone volume (BV), bone mineral density (BMD), trabecular thickness (TbTh), and trabecular spacing (TbSp) were evaluated by microcomputed tomography (µ-CT), and a histomorphometric analysis was performed to evaluate the new bone formation area. The expression of osteogenic markers, such as bone sialoprotein (BSP) and osteocalcin, were evaluated by immunohistochemistry (IHC). The ABB/high BMP group showed significantly higher BV than the ABB/low BMP (p = 0.004) and control groups (p = 0.000) and higher TbTh than the control group (p = 0.000). The ABB/low BMP group showed significantly higher BV, BMD, and TbTh than the control group (p = 0.002, 0.042, and 0.000, respectively). The histomorphometry showed significantly higher bone formation in the ABB/low and high BMP groups than in the control group (p = 0.000). IHC showed a high expression of BSP and osteocalcin in the ABB/low and high BMP groups. Subperiosteal bone grafts with ABB and rhBMP-2 have not been studied. In our study, we confirmed that rhBMP-2 contributes to new bone formation in a subperiosteal bone graft with ABB.

Evaluation of Gamma Irradiated Human Bone in Nude Rats

2005 ◽  
Vol 288-289 ◽  
pp. 269-272
Author(s):  
Y. Yu ◽  
Jin Biao Chen ◽  
J.L. Yang ◽  
D.A.F. Morgan ◽  
W.R. Walsh
Keyword(s):  
Bone Formation ◽  
Gamma Irradiation ◽  
Soft Tissues ◽  
Bone Grafts ◽  
Human Bone ◽  
New Bone Formation ◽  
Nude Rats ◽  
Defect Area ◽  
Post Operation ◽  
Gamma Irradiated

Deep-frozen morselized human bone grafts showed osteoconductivity and osteoinductivity when implanted into tibial window defects of nude rats. The osteoconductivity was assessed by measuring the total area of newly formed bone bridged by the implanted bone grafts in the entire defect area. The osteoinductivity was evidenced by the presence of active osteoblast-like cells and new bone formation around the implanted bone grafts, which were surrounded by soft tissues distant from the host cortex. Gamma irradiation at the doses of 15 or 25 kGray reduced the osteoconductivity (ANOVA and LSD tests, p<0.05) at 3 weeks post operation. The 25 kGray group had a significantly lower level of new bone formation compared with the 0 and 15 kGray groups. The evidences of osteoinductivity were only noted in the 0 and 15 kGray groups. Our data indicate that 25 kGray gamma irradiation reduces the osteoconductive and osteoinductive properties of the morselized human bone graft.

Osteogenesis and new bone formation of alendronate-immobilized porous PLGA microspheres in a rat calvarial defect model

2017 ◽  
Vol 52 ◽  
pp. 277-286 ◽  
Author(s):  
Jae Yong Lee ◽  
Sung Eun Kim ◽  
Young-Pil Yun ◽  
Sung-Wook Choi ◽  
Daniel I. Jeon ◽  
...  
Keyword(s):  
Bone Formation ◽  
Plga Microspheres ◽  
Calvarial Defect ◽  
New Bone Formation ◽  
Defect Model ◽  
Rat Calvarial Defect

Tissue Engineering Bone Formation in Novel Recombinant Human Bone Morphogenic Protein 2–Atelocollagen Composite Scaffolds

2007 ◽  
Vol 78 (2) ◽  
pp. 335-343 ◽  
Author(s):  
Lein-Tuan Hou ◽  
Cheng-Meei Liu ◽  
Bu-Yuan Liu ◽  
Po-Chun Chang ◽  
Min-Huei Chen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Formation ◽  
Bone Morphogenic Protein ◽  
Human Bone ◽  
Composite Scaffolds ◽  
Bone Morphogenic Protein 2 ◽  
Recombinant Human Bone

scholarly journals Fabrication and Evaluation of Porous Beta-Tricalcium Phosphate/Hydroxyapatite (60/40) Composite as a Bone Graft Extender Using Rat Calvarial Bone Defect Model

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Jae Hyup Lee ◽  
Mi Young Ryu ◽  
Hae-Ri Baek ◽  
Kyung Mee Lee ◽  
Jun-Hyuk Seo ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Tricalcium Phosphate ◽  
Calvarial Defect ◽  
Micro Ct ◽  
Porous Substrate ◽  
New Bone Formation ◽  
Defect Model ◽  
Beta Tricalcium Phosphate ◽  
Bone Graft Extender

Beta-tricalcium phosphate (β-TCP) and hydroxyapatite (HA) are widely used as bone graft extenders due to their osteoconductivity and high bioactivity. This study aims to evaluate the possibility of using porous substrate with composite ceramics (β-TCP: HA = 60% : 40%, 60TCP40HA) as a bone graft extender and comparing it with Bio-Oss. Interconnectivity and macroporosity ofβ-TCP porous substrate were 99.9% and 83%, respectively, and the macro-porosity of packed granule after crushing was 69%. Calvarial defect model with 8 mm diameter was generated with male Sprague-Dawley rats and 60TCP40HA was implanted. Bio-Oss was implanted for a control group and micro-CT and histology were performed at 4 and 8 weeks after implantation. The 60TCP40HA group showed better new bone formation than the Bio-Oss group and the bone formation at central area of bone defect was increased at 8 weeks in micro-CT and histology. The percent bone volume and trabecular number of the 60TCP40HA group were significantly higher than those of Bio-Oss group. This study confirms the usefulness of the porous 60TCP40HA composite as a bone graft extender by showing increased new bone formation in the calvarial defect model and improved bone formation both quantitatively and qualitatively when compared to Bio-Oss.

scholarly journals Settable Polymeric Autograft Extenders in a Rabbit Radius Model of Bone Formation

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 3960
Author(s):  
Lauren A. Boller ◽  
Madison A.P. McGough ◽  
Stefanie M. Shiels ◽  
Craig L. Duvall ◽  
Joseph C. Wenke ◽  
...  
Keyword(s):  
Bone Formation ◽  
Gold Standard ◽  
Bone Growth ◽  
Bone Grafts ◽  
Polymer Composition ◽  
Cellular Infiltration ◽  
New Bone Formation ◽  
Defect Model ◽  
Patient Morbidity

Autograft (AG) is the gold standard for bone grafts, but limited quantities and patient morbidity are associated with its use. AG extenders have been proposed to minimize the volume of AG while maintaining the osteoinductive properties of the implant. In this study, poly(ester urethane) (PEUR) and poly(thioketal urethane) (PTKUR) AG extenders were implanted in a 20-mm rabbit radius defect model to evaluate new bone formation and graft remodeling. Outcomes including µCT and histomorphometry were measured at 12 weeks and compared to an AG (no polymer) control. AG control examples exhibited new bone formation, but inconsistent healing was observed. The implanted AG control was resorbed by 12 weeks, while AG extenders maintained implanted AG throughout the study. Bone growth from the defect interfaces was observed in both AG extenders, but residual polymer inhibited cellular infiltration and subsequent bone formation within the center of the implant. PEUR-AG extenders degraded more rapidly than PTKUR-AG extenders. These observations demonstrated that AG extenders supported new bone formation and that polymer composition did not have an effect on overall bone formation. Furthermore, the results indicated that early cellular infiltration is necessary for harnessing the osteoinductive capabilities of AG.

Investigation of the effects of semaphorin 3A on new bone formation in a rat calvarial defect model

2019 ◽  
Vol 47 (3) ◽  
pp. 473-483 ◽  
Author(s):  
Sevinç Kenan ◽  
Özen Doğan Onur ◽  
Seyhun Solakoğlu ◽  
Tuğba Kotil ◽  
Mustafa Ramazanoğlu ◽  
...  
Keyword(s):  
Bone Formation ◽  
Calvarial Defect ◽  
Semaphorin 3A ◽  
New Bone Formation ◽  
Defect Model ◽  
Rat Calvarial Defect
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