scholarly journals Whey as an Alternative Nutrient Medium for Growth of Sporosarcina pasteurii and Its Effect on CaCO3 Polymorphism and Fly Ash Bioconsolidation

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2470
Author(s):  
Sandra Chaparro ◽  
Hugo A. Rojas ◽  
Gerardo Caicedo ◽  
Gustavo Romanelli ◽  
Antonio Pineda ◽  
...  
Keyword(s):  
Fly Ash ◽  
Calcium Carbonate ◽  
Calcium Carbonate Precipitation ◽  
Aquatic Life ◽  
Sporosarcina Pasteurii ◽  
Crystal Polymorphism ◽  
Potential Applications ◽  
The Media ◽  
Ft Ir ◽  
Economical Alternative

Whey in large quantities can cause environmental problems when discarded, because it reduces dissolved oxygen and aquatic life. Nonetheless, it could be used as an easily available and economical alternative to reduce culture medium costs in microbially induced calcium carbonate precipitation (MICP). In this work, a native Sporosarcina pasteurii was isolated and then cultured by using different proportions of whey (W) in nutrient broth (NB). The solids were characterized by XRD, FT-IR, TGA, and SEM. The potential applications in bioconsolidation were also studied. Whey concentration was directly related to CaCO3 production. Higher whey concentrations reduced calcium carbonate purity to nearly 80%. All experiments showed calcite and vaterite fractions, where a whey increment in the media increased calcite content and decreased vaterite content, causing a decrease in crystal size. MICP improved compressive strength (CS) in sand and fly ash. The best CS results were obtained by fly ash treated with 25 W-75 NB (37.2 kPa) and sand with 75 W-25 NB (32.1 kPa). Whey changed crystal polymorphism in biogenic CaCO3 production. Material bioconsolidation depends on the CaCO3 polymorph, thus fly ash was effectively bioconsolidated by crystallization of vaterite and sand by crystallization of calcite.

scholarly journals Real-time monitoring of calcification process by Sporosarcina pasteurii biofilm

The Analyst ◽  
2016 ◽  
Vol 141 (10) ◽  
pp. 2887-2895 ◽  
Author(s):  
Dustin Harris ◽  
Jyothir Ganesh Ummadi ◽  
Andrew R. Thurber ◽  
Yvan Allau ◽  
Circe Verba ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Real Time ◽  
Carbonate Precipitation ◽  
Real Time Monitoring ◽  
Calcium Carbonate Precipitation ◽  
Sporosarcina Pasteurii ◽  
Scanning Electrochemical Microscope ◽  
Calcification Process

Chemical and morphological mapping of live bacterial assisted calcium carbonate precipitation using scanning electrochemical microscope (SECM).

scholarly journals A Numerical Model for Enzymatically Induced Calcium Carbonate Precipitation

2020 ◽  
Vol 10 (13) ◽  
pp. 4538 ◽  
Author(s):  
Johannes Hommel ◽  
Arda Akyel ◽  
Zachary Frieling ◽  
Adrienne J. Phillips ◽  
Robin Gerlach ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Numerical Model ◽  
Model Calibration ◽  
Column Experiments ◽  
Carbonate Precipitation ◽  
Carbonate Mineral ◽  
Calcium Carbonate Precipitation ◽  
Mineral Precipitation ◽  
Sporosarcina Pasteurii

Enzymatically induced calcium carbonate precipitation (EICP) is an emerging engineered mineralization method similar to others such as microbially induced calcium carbonate precipitation (MICP). EICP is advantageous compared to MICP as the enzyme is still active at conditions where microbes, e.g., Sporosarcina pasteurii, commonly used for MICP, cannot grow. Especially, EICP expands the applicability of ureolysis-induced calcium carbonate mineral precipitation to higher temperatures, enabling its use in leakage mitigation deeper in the subsurface than previously thought to be possible with MICP. A new conceptual and numerical model for EICP is presented. The model was calibrated and validated using quasi-1D column experiments designed to provide the necessary data for model calibration and can now be used to assess the potential of EICP applications for leakage mitigation and other subsurface modifications.

scholarly journals Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Raja Murugan ◽  
G. K. Suraishkumar ◽  
Abhijit Mukherjee ◽  
Navdeep K. Dhami
Keyword(s):  
Microbial Community ◽  
Calcium Carbonate ◽  
Kinetic Analysis ◽  
Microbial Communities ◽  
Slow Rate ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Promising Technique ◽  
Sporosarcina Pasteurii ◽  
The Impact

AbstractMicrobially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including biostimulation and bioaugmentation. Although bioaugmentation strategy using efficient ureolytic biocementing culture of Sporosarcina pasteurii is widely practiced, the impact of native ureolytic microbial communities (NUMC) on CaCO3 mineralisation via S. pasteurii has not been explored. In this paper, we investigated the effect of different concentrations of NUMC on MICP kinetics and biomineral properties in the presence and absence of S. pasteurii. Kinetic analysis showed that the biocementation potential of S. pasteurii is sixfold higher than NUMC and is not significantly impacted even when the concentration of the NUMC is eight times higher. Micrographic results revealed a quick rate of CaCO3 precipitation by S. pasteurii leading to generation of smaller CaCO3 crystals (5–40 µm), while slow rate of CaCO3 precipitation by NUMC led to creation of larger CaCO3 crystals (35–100 µm). Mineralogical results showed the predominance of calcite phase in both sets. The outcome of current study is crucial for tailor-made applications of MICP.

scholarly journals Influence of Native Ureolytic Microbial Community on Biocementation Potential of Sporosarcina Pasteurii

2021 ◽  
Author(s):  
Raja Murugan ◽  
G. K. Suraishkumar ◽  
Abhijit Muhkerjee ◽  
Navdeep K Dhami
Keyword(s):  
Microbial Community ◽  
Calcium Carbonate ◽  
Kinetic Analysis ◽  
Microbial Communities ◽  
Slow Rate ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Promising Technique ◽  
Sporosarcina Pasteurii ◽  
The Impact

Abstract Microbially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including biostimulation and bioaugmentation. Although bioaugmentation strategy using efficient ureolytic biocementing culture of Sporosarcina pasteurii is widely practiced, the impact of native ureolytic microbial communities (NUMC) on CaCO3 mineralisation via S. pasteurii has not been explored. In this paper, we investigated the effect of different concentrations of NUMC on MICP kinetics and biomineral properties in the presence and absence of S. pasteurii. Kinetic analysis showed that the biocementation potential of S. pasteurii is 6-fold higher than the NUMC and is not significantly impacted even when the concentration of the NUMC is eight times higher. Micrographic results revealed a quick rate of CaCO3 precipitation by S. pasteurii led to the generation of smaller CaCO3 crystals (5–40 µm), while the slow rate of CaCO3 precipitation by NUMC led to the creation of larger CaCO3 crystals (35–100 µm). Mineralogical results showed the predominance of the calcite phase in both sets. The outcome of the current study is crucial for tailor-made applications of MICP.

scholarly journals Transcriptome analyses reveal the utilization of nitrogen sources and related metabolic mechanisms of Sporosarcina pasteurii

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246818
Author(s):  
Di Pei ◽  
Zhiming Liu ◽  
Wenjian Wu ◽  
Biru Hu
Keyword(s):  
Calcium Carbonate ◽  
Raw Materials ◽  
Atp Synthesis ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Nitrogen Utilization ◽  
Calcium Carbonate Precipitation ◽  
Synthesis Mechanism ◽  
Sporosarcina Pasteurii ◽  
Transcriptome Analyses

In recent years, Sporosarcina pasteurii (S. pasteurii) has become one of the most popular bacteria in microbially induced calcium carbonate precipitation (MICP). Various applications have been developed based on the efficient urease that can induce the precipitation of calcium carbonate. However, the metabolic mechanism related to biomineralization of S. pasteurii has not been clearly elucidated. The process of bacterial culture and biomineralization consumes a large amount of urea or ammonium salts, which are usually used as agricultural fertilizers, not to mention probable environmental pollutions caused by the excessive use of these raw materials. Therefore, it is urgent to reveal the mechanism of nitrogen utilization and metabolism of S. pasteurii. In this paper, we compared the growth and gene expression of S. pasteurii under three different culture conditions through transcriptome analyses. GO and KEGG analyses revealed that both ammonium and urea were direct nitrogen sources of S. pasteurii, and the bacteria could not grow normally in the absence of ammonium or urea. To the best of our knowledge, this paper is the first one to reveal the nitrogen utilization mechanism of S. pasteurii through transcriptome methods. Furthermore, the presence of ammonium might promote the synthesis of intracellular ATP and enhance the motility of the bacteria. There should be an ATP synthesis mechanism associated with urea hydrolysis catalyzed by urease in S. pasteurii.

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