scholarly journals Real-time monitoring of calcification process by Sporosarcina pasteurii biofilm

The Analyst ◽  
2016 ◽  
Vol 141 (10) ◽  
pp. 2887-2895 ◽  
Author(s):  
Dustin Harris ◽  
Jyothir Ganesh Ummadi ◽  
Andrew R. Thurber ◽  
Yvan Allau ◽  
Circe Verba ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Real Time ◽  
Carbonate Precipitation ◽  
Real Time Monitoring ◽  
Calcium Carbonate Precipitation ◽  
Sporosarcina Pasteurii ◽  
Scanning Electrochemical Microscope ◽  
Calcification Process

Chemical and morphological mapping of live bacterial assisted calcium carbonate precipitation using scanning electrochemical microscope (SECM).

scholarly journals Microbial-induced calcium carbonate precipitation: an experimental toolbox for in situ and real time investigation of micro-scale pH evolution

RSC Advances ◽  
2020 ◽  
Vol 10 (35) ◽  
pp. 20485-20493
Author(s):  
Jennifer Zehner ◽  
Anja Røyne ◽  
Alexander Wentzel ◽  
Pawel Sikorski
Keyword(s):  
Calcium Carbonate ◽  
Real Time ◽  
Experimental Methods ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Micro Scale ◽  
Ph Changes ◽  
Global And Local ◽  
Local Ph

We present two novel experimental methods to follow global and local pH changes on a microscale in bio-cementation processes.

scholarly journals Microbial-induced calcium carbonate precipitation: An experimental toolbox for in situ and real-time investigation of micro-scale pH evolution

2020 ◽  
Author(s):  
Jennifer Zehner ◽  
Anja Røyne ◽  
Alexander Wentzel ◽  
Pawel Sikorski
Keyword(s):  
Calcium Carbonate ◽  
Real Time ◽  
Small Sample ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Micro Scale ◽  
Ph Changes ◽  
Macro Scale ◽  
Local Ph

AbstractConcrete is the second most consumed product by humans, after water. However, the production of cement, which is used as a binding material in concrete, causes more than 5% of anthropogenic CO2 emissions and has therefore a significant contribution to climate change and global warming. Due to increasing environmental awareness and international climate goals, there is a need for emission-reduced materials, that can replace conventional concrete in certain applications. One path to produce a solid, concrete-like construction material is microbial-induced calcium carbonate precipitation (MICP). As a calcium source in MICP, crushed limestone, which mainly consists out of CaCO3, can be dissolved with acids, for example lactic acid. The pH evolution during crystallization and dissolution processes provides important information about kinetics of the reactions. However, previous research on MICP has mainly been focused on macro-scale pH evolution and on characterization of the finished material. To get a better understanding of MICP it is important to be able to follow also local pH changes in a sample. In this work we present a new method to study processes of MICP at micro-scale in situ and in real time. We present two different methods to monitor the pH changes during the precipitation process of CaCO3. In the first method, the average pHs of small sample volumes are measured in real time, and pH changes are subsequently correlated with processes in the sample by comparing to optical microscope results. The second method is introduced to follow local pH changes at a grain scale in situ and in real time. Furthermore, local pH changes during the dissolution of CaCO3 crystals are monitored. We demonstrate that these two methods are powerful tools to investigate pH changes for both MICP precipitation and CaCO3 dissolution for knowledge-based improvement of MICP-based material properties.Graphical TOC Entry

scholarly journals A Numerical Model for Enzymatically Induced Calcium Carbonate Precipitation

2020 ◽  
Vol 10 (13) ◽  
pp. 4538 ◽  
Author(s):  
Johannes Hommel ◽  
Arda Akyel ◽  
Zachary Frieling ◽  
Adrienne J. Phillips ◽  
Robin Gerlach ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Numerical Model ◽  
Model Calibration ◽  
Column Experiments ◽  
Carbonate Precipitation ◽  
Carbonate Mineral ◽  
Calcium Carbonate Precipitation ◽  
Mineral Precipitation ◽  
Sporosarcina Pasteurii

Enzymatically induced calcium carbonate precipitation (EICP) is an emerging engineered mineralization method similar to others such as microbially induced calcium carbonate precipitation (MICP). EICP is advantageous compared to MICP as the enzyme is still active at conditions where microbes, e.g., Sporosarcina pasteurii, commonly used for MICP, cannot grow. Especially, EICP expands the applicability of ureolysis-induced calcium carbonate mineral precipitation to higher temperatures, enabling its use in leakage mitigation deeper in the subsurface than previously thought to be possible with MICP. A new conceptual and numerical model for EICP is presented. The model was calibrated and validated using quasi-1D column experiments designed to provide the necessary data for model calibration and can now be used to assess the potential of EICP applications for leakage mitigation and other subsurface modifications.

scholarly journals Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Raja Murugan ◽  
G. K. Suraishkumar ◽  
Abhijit Mukherjee ◽  
Navdeep K. Dhami
Keyword(s):  
Microbial Community ◽  
Calcium Carbonate ◽  
Kinetic Analysis ◽  
Microbial Communities ◽  
Slow Rate ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Promising Technique ◽  
Sporosarcina Pasteurii ◽  
The Impact

AbstractMicrobially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including biostimulation and bioaugmentation. Although bioaugmentation strategy using efficient ureolytic biocementing culture of Sporosarcina pasteurii is widely practiced, the impact of native ureolytic microbial communities (NUMC) on CaCO3 mineralisation via S. pasteurii has not been explored. In this paper, we investigated the effect of different concentrations of NUMC on MICP kinetics and biomineral properties in the presence and absence of S. pasteurii. Kinetic analysis showed that the biocementation potential of S. pasteurii is sixfold higher than NUMC and is not significantly impacted even when the concentration of the NUMC is eight times higher. Micrographic results revealed a quick rate of CaCO3 precipitation by S. pasteurii leading to generation of smaller CaCO3 crystals (5–40 µm), while slow rate of CaCO3 precipitation by NUMC led to creation of larger CaCO3 crystals (35–100 µm). Mineralogical results showed the predominance of calcite phase in both sets. The outcome of current study is crucial for tailor-made applications of MICP.

scholarly journals Influence of Native Ureolytic Microbial Community on Biocementation Potential of Sporosarcina Pasteurii

2021 ◽  
Author(s):  
Raja Murugan ◽  
G. K. Suraishkumar ◽  
Abhijit Muhkerjee ◽  
Navdeep K Dhami
Keyword(s):  
Microbial Community ◽  
Calcium Carbonate ◽  
Kinetic Analysis ◽  
Microbial Communities ◽  
Slow Rate ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Promising Technique ◽  
Sporosarcina Pasteurii ◽  
The Impact

Abstract Microbially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including biostimulation and bioaugmentation. Although bioaugmentation strategy using efficient ureolytic biocementing culture of Sporosarcina pasteurii is widely practiced, the impact of native ureolytic microbial communities (NUMC) on CaCO3 mineralisation via S. pasteurii has not been explored. In this paper, we investigated the effect of different concentrations of NUMC on MICP kinetics and biomineral properties in the presence and absence of S. pasteurii. Kinetic analysis showed that the biocementation potential of S. pasteurii is 6-fold higher than the NUMC and is not significantly impacted even when the concentration of the NUMC is eight times higher. Micrographic results revealed a quick rate of CaCO3 precipitation by S. pasteurii led to the generation of smaller CaCO3 crystals (5–40 µm), while the slow rate of CaCO3 precipitation by NUMC led to the creation of larger CaCO3 crystals (35–100 µm). Mineralogical results showed the predominance of the calcite phase in both sets. The outcome of the current study is crucial for tailor-made applications of MICP.

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