scholarly journals Comparative Study on the Sand Bioconsolidation through Calcium Carbonate Precipitation by Sporosarcina pasteurii and Bacillus subtilis

Crystals ◽  
2018 ◽  
Vol 8 (5) ◽  
pp. 189 ◽  
Author(s):  
Chun-Mei Hsu ◽  
Yi-Hsun Huang ◽  
Vanita Nimje ◽  
Wen-Chien Lee ◽  
How-Ji Chen ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Calcium Carbonate ◽  
Comparative Study ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Sporosarcina Pasteurii

scholarly journals Real-time monitoring of calcification process by Sporosarcina pasteurii biofilm

The Analyst ◽  
2016 ◽  
Vol 141 (10) ◽  
pp. 2887-2895 ◽  
Author(s):  
Dustin Harris ◽  
Jyothir Ganesh Ummadi ◽  
Andrew R. Thurber ◽  
Yvan Allau ◽  
Circe Verba ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Real Time ◽  
Carbonate Precipitation ◽  
Real Time Monitoring ◽  
Calcium Carbonate Precipitation ◽  
Sporosarcina Pasteurii ◽  
Scanning Electrochemical Microscope ◽  
Calcification Process

Chemical and morphological mapping of live bacterial assisted calcium carbonate precipitation using scanning electrochemical microscope (SECM).

scholarly journals A Numerical Model for Enzymatically Induced Calcium Carbonate Precipitation

2020 ◽  
Vol 10 (13) ◽  
pp. 4538 ◽  
Author(s):  
Johannes Hommel ◽  
Arda Akyel ◽  
Zachary Frieling ◽  
Adrienne J. Phillips ◽  
Robin Gerlach ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Numerical Model ◽  
Model Calibration ◽  
Column Experiments ◽  
Carbonate Precipitation ◽  
Carbonate Mineral ◽  
Calcium Carbonate Precipitation ◽  
Mineral Precipitation ◽  
Sporosarcina Pasteurii

Enzymatically induced calcium carbonate precipitation (EICP) is an emerging engineered mineralization method similar to others such as microbially induced calcium carbonate precipitation (MICP). EICP is advantageous compared to MICP as the enzyme is still active at conditions where microbes, e.g., Sporosarcina pasteurii, commonly used for MICP, cannot grow. Especially, EICP expands the applicability of ureolysis-induced calcium carbonate mineral precipitation to higher temperatures, enabling its use in leakage mitigation deeper in the subsurface than previously thought to be possible with MICP. A new conceptual and numerical model for EICP is presented. The model was calibrated and validated using quasi-1D column experiments designed to provide the necessary data for model calibration and can now be used to assess the potential of EICP applications for leakage mitigation and other subsurface modifications.

scholarly journals Engineered ureolytic Bacillus subtilis and its future in Microbial Induced Calcium Carbonate Precipitation (MICCP)

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Jennifer Wright ◽  
Polly Moreland ◽  
Anil Wipat ◽  
Meng Zhang ◽  
Martyn Dade-Robertson
Keyword(s):  
Bacillus Subtilis ◽  
Calcium Carbonate ◽  
Environmental Remediation ◽  
Construction Materials ◽  
Carbonate Precipitation ◽  
Natural Setting ◽  
Calcium Carbonate Precipitation ◽  
Surrounding Matrix ◽  
Low Efficiency ◽  
Ground Stability

As the global population grows there is an urgent need for increased, yet sustainable civil infrastructure. The ability to harness biological processes in order to improve ground stability; as well as creating construction materials without adding to climate damage is necessary. In almost every environment on earth, microorganisms and microbially mediated mineralisation (biomineralisation) processes are active. It is well documented that microbes present in soil can induce the precipitation of calcium carbonate (CaCO3) in both the laboratory and the natural setting through microbial induced calcium carbonate precipitation (MICCP). MICCP utilises microorganisms as a result of their active metabolism, to precipitate CaCO3, strengthening the surrounding matrix. MICCP is used in a variety of different applications such as carbon sequestration, environmental remediation and improving construction materials. The enzyme urease catalyzes the hydrolysis of urea to ammonia and CO2, and is acknowledged to be instrumental in MICCP. Bacillus subtilis is a model, gram positive, spore-forming soil bacterium that produces a functionally active urease, but with low efficiency, and the activation is not completely understood. Sporosarcina pasteurii is one of the most commonly used MICCP microbes as its urease operon has been well studied and the bacterium has proven to produce ecologically stable bioconstruction materials. The ability to clone the urease operon of S. pasteurii into the model B. subtilis would create an engineered ureolytic organism whose urease activity could be controlled. This control would enable the CaCO3 morphology and material properties to be tailored and would create a truly responsive biomaterial.

scholarly journals Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Raja Murugan ◽  
G. K. Suraishkumar ◽  
Abhijit Mukherjee ◽  
Navdeep K. Dhami
Keyword(s):  
Microbial Community ◽  
Calcium Carbonate ◽  
Kinetic Analysis ◽  
Microbial Communities ◽  
Slow Rate ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Promising Technique ◽  
Sporosarcina Pasteurii ◽  
The Impact

AbstractMicrobially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including biostimulation and bioaugmentation. Although bioaugmentation strategy using efficient ureolytic biocementing culture of Sporosarcina pasteurii is widely practiced, the impact of native ureolytic microbial communities (NUMC) on CaCO3 mineralisation via S. pasteurii has not been explored. In this paper, we investigated the effect of different concentrations of NUMC on MICP kinetics and biomineral properties in the presence and absence of S. pasteurii. Kinetic analysis showed that the biocementation potential of S. pasteurii is sixfold higher than NUMC and is not significantly impacted even when the concentration of the NUMC is eight times higher. Micrographic results revealed a quick rate of CaCO3 precipitation by S. pasteurii leading to generation of smaller CaCO3 crystals (5–40 µm), while slow rate of CaCO3 precipitation by NUMC led to creation of larger CaCO3 crystals (35–100 µm). Mineralogical results showed the predominance of calcite phase in both sets. The outcome of current study is crucial for tailor-made applications of MICP.

scholarly journals A Bacillus subtilis cell fraction (BCF) inducing calcium carbonate precipitation: Biotechnological perspectives for monumental stone reinforcement

2014 ◽  
Vol 15 (4) ◽  
pp. 345-351 ◽  
Author(s):  
Brunella Perito ◽  
Massimiliano Marvasi ◽  
Chiara Barabesi ◽  
Giorgio Mastromei ◽  
Susanna Bracci ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Calcium Carbonate ◽  
Cell Fraction ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Subtilis Cell

scholarly journals Influence of Native Ureolytic Microbial Community on Biocementation Potential of Sporosarcina Pasteurii

2021 ◽  
Author(s):  
Raja Murugan ◽  
G. K. Suraishkumar ◽  
Abhijit Muhkerjee ◽  
Navdeep K Dhami
Keyword(s):  
Microbial Community ◽  
Calcium Carbonate ◽  
Kinetic Analysis ◽  
Microbial Communities ◽  
Slow Rate ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Promising Technique ◽  
Sporosarcina Pasteurii ◽  
The Impact

Abstract Microbially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including biostimulation and bioaugmentation. Although bioaugmentation strategy using efficient ureolytic biocementing culture of Sporosarcina pasteurii is widely practiced, the impact of native ureolytic microbial communities (NUMC) on CaCO3 mineralisation via S. pasteurii has not been explored. In this paper, we investigated the effect of different concentrations of NUMC on MICP kinetics and biomineral properties in the presence and absence of S. pasteurii. Kinetic analysis showed that the biocementation potential of S. pasteurii is 6-fold higher than the NUMC and is not significantly impacted even when the concentration of the NUMC is eight times higher. Micrographic results revealed a quick rate of CaCO3 precipitation by S. pasteurii led to the generation of smaller CaCO3 crystals (5–40 µm), while the slow rate of CaCO3 precipitation by NUMC led to the creation of larger CaCO3 crystals (35–100 µm). Mineralogical results showed the predominance of the calcite phase in both sets. The outcome of the current study is crucial for tailor-made applications of MICP.

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