scholarly journals In-Vivo Degradation Behavior and Osseointegration of 3D Powder-Printed Calcium Magnesium Phosphate Cement Scaffolds

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 946
Author(s):  
Katharina Kowalewicz ◽  
Elke Vorndran ◽  
Franziska Feichtner ◽  
Anja-Christina Waselau ◽  
Manuel Brueckner ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Bone Substitutes ◽  
Magnesium Phosphate ◽  
Degradation Behavior ◽  
Micro Computed Tomography ◽  
Calcium Magnesium ◽  
Interest In Research ◽  
In Vivo Degradation ◽  
Volume Decrease

Calcium magnesium phosphate cements (CMPCs) are promising bone substitutes and experience great interest in research. Therefore, in-vivo degradation behavior, osseointegration and biocompatibility of three-dimensional (3D) powder-printed CMPC scaffolds were investigated in the present study. The materials Mg225 (Ca0.75Mg2.25(PO4)2) and Mg225d (Mg225 treated with diammonium hydrogen phosphate (DAHP)) were implanted as cylindrical scaffolds (h = 5 mm, Ø = 3.8 mm) in both lateral femoral condyles in rabbits and compared with tricalcium phosphate (TCP). Treatment with DAHP results in the precipitation of struvite, thus reducing pore size and overall porosity and increasing pressure stability. Over 6 weeks, the scaffolds were evaluated clinically, radiologically, with Micro-Computed Tomography (µCT) and histological examinations. All scaffolds showed excellent biocompatibility. X-ray and in-vivo µCT examinations showed a volume decrease and increasing osseointegration over time. Structure loss and volume decrease were most evident in Mg225. Histologically, all scaffolds degraded centripetally and were completely traversed by new bone, in which the remaining scaffold material was embedded. While after 6 weeks, Mg225d and TCP were still visible as a network, only individual particles of Mg225 were present. Based on these results, Mg225 and Mg225d appear to be promising bone substitutes for various loading situations that should be investigated further.

scholarly journals Heidelberg-mCT-Analyzer: a novel method for standardized microcomputed-tomography-guided evaluation of scaffold properties in bone and tissue research

2015 ◽  
Vol 2 (11) ◽  
pp. 150496 ◽  
Author(s):  
Fabian Westhauser ◽  
Christian Weis ◽  
Melanie Hoellig ◽  
Tyler Swing ◽  
Gerhard Schmidmaier ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Characteristic Parameter ◽  
Micro Computed Tomography ◽  
Mineral Density ◽  
Independent Analysis ◽  
Scaffold Properties

Bone tissue engineering and bone scaffold development represent two challenging fields in orthopaedic research. Micro-computed tomography (mCT) allows non-invasive measurement of these scaffolds’ properties in vivo . However, the lack of standardized mCT analysis protocols and, therefore, the protocols’ user-dependency make interpretation of the reported results difficult. To overcome these issues in scaffold research, we introduce the Heidelberg-mCT-Analyzer. For evaluation of our technique, we built 10 bone-inducing scaffolds, which underwent mCT acquisition before ectopic implantation (T0) in mice, and at explantation eight weeks thereafter (T1). The scaffolds’ three-dimensional reconstructions were automatically segmented using fuzzy clustering with fully automatic level-setting. The scaffold itself and its pores were then evaluated for T0 and T1. Analysing the scaffolds’ characteristic parameter set with our quantification method showed bone formation over time. We were able to demonstrate that our algorithm obtained the same results for basic scaffold parameters (e.g. scaffold volume, pore number and pore volume) as other established analysis methods. Furthermore, our algorithm was able to analyse more complex parameters, such as pore size range, tissue mineral density and scaffold surface. Our imaging and post-processing strategy enables standardized and user-independent analysis of scaffold properties, and therefore is able to improve the quantitative evaluations of scaffold-associated bone tissue-engineering projects.

In vivo degradation of low temperature calcium and magnesium phosphate ceramics in a heterotopic model

2011 ◽  
Vol 7 (9) ◽  
pp. 3469-3475 ◽  
Author(s):  
Uwe Klammert ◽  
Anita Ignatius ◽  
Uwe Wolfram ◽  
Tobias Reuther ◽  
Uwe Gbureck
Keyword(s):  
Low Temperature ◽  
Magnesium Phosphate ◽  
Calcium And Magnesium ◽  
In Vivo Degradation

scholarly journals In vivo degradation of three-dimensional silk fibroin scaffolds

Biomaterials ◽  
2008 ◽  
Vol 29 (24-25) ◽  
pp. 3415-3428 ◽  
Author(s):  
Yongzhong Wang ◽  
Darya D. Rudym ◽  
Ashley Walsh ◽  
Lauren Abrahamsen ◽  
Hyeon-Joo Kim ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Three Dimensional ◽  
In Vivo Degradation

In vivo degradation behavior of Ca-deficient hydroxyapatite coated Mg–Zn–Ca alloy for bone implant application

2011 ◽  
Vol 88 (1) ◽  
pp. 254-259 ◽  
Author(s):  
Huanxin Wang ◽  
Shaokang Guan ◽  
Yisheng Wang ◽  
Hongjian Liu ◽  
Haitao Wang ◽  
...  
Keyword(s):  
Degradation Behavior ◽  
Bone Implant ◽  
In Vivo Degradation

scholarly journals In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds

2020 ◽  
Vol 24 (1) ◽  
Author(s):  
Naghmeh Abbasi ◽  
Ryan S. B. Lee ◽  
Saso Ivanovski ◽  
Robert M. Love ◽  
Stephen Hamlet
Keyword(s):  
Bone Regeneration ◽  
Size Structure ◽  
Three Dimensional ◽  
Immunohistochemical Analysis ◽  
Repair Process ◽  
Micro Computed Tomography ◽  
Calvarial Defects ◽  
Promising Solution ◽  
Poly Caprolactone

Abstract Background Biomaterial-based bone tissue engineering represents a promising solution to overcome reduced residual bone volume. It has been previously demonstrated that gradient and offset architectures of three-dimensional melt electrowritten poly-caprolactone (PCL) scaffolds could successfully direct osteoblast cells differentiation toward an osteogenic lineage, resulting in mineralization. The aim of this study was therefore to evaluate the in vivo osteoconductive capacity of PCL scaffolds with these different architectures. Methods Five different calcium phosphate (CaP) coated melt electrowritten PCL pore sized scaffolds: 250 μm and 500 μm, 500 μm with 50% fibre offset (offset.50.50), tri layer gradient 250–500-750 μm (grad.250top) and 750–500-250 μm (grad.750top) were implanted into rodent critical-sized calvarial defects. Empty defects were used as a control. After 4 and 8 weeks of healing, the new bone was assessed by micro-computed tomography and immunohistochemistry. Results Significantly more newly formed bone was shown in the grad.250top scaffold 8 weeks post-implantation. Histological investigation also showed that soft tissue was replaced with newly formed bone and fully covered the grad.250top scaffold. While, the bone healing did not happen completely in the 250 μm, offset.50.50 scaffolds and blank calvaria defects following 8 weeks of implantation. Immunohistochemical analysis showed the expression of osteogenic markers was present in all scaffold groups at both time points. The mineralization marker Osteocalcin was detected with the highest intensity in the grad.250top and 500 μm scaffolds. Moreover, the expression of the endothelial markers showed that robust angiogenesis was involved in the repair process. Conclusions These results suggest that the gradient pore size structure provides superior conditions for bone regeneration.

scholarly journals Supplementation with 45S5 Bioactive Glass Reduces In Vivo Resorption of the β-Tricalcium-Phosphate-Based Bone Substitute Material Vitoss

2019 ◽  
Vol 20 (17) ◽  
pp. 4253 ◽  
Author(s):  
Fabian Westhauser ◽  
Christopher Essers ◽  
Maria Karadjian ◽  
Bruno Reible ◽  
Gerhard Schmidmaier ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Tricalcium Phosphate ◽  
Bone Substitute ◽  
Degradation Kinetics ◽  
Bone Substitutes ◽  
Micro Computed Tomography ◽  
Immunodeficient Mice ◽  
Bone Substitute Materials ◽  
Osseous Regeneration

Compared to other materials such as 45S5 bioactive glass (BG), β-tricalcium phosphate (β-TCP)-based bone substitutes such as Vitoss show limited material-driven stimulation of osteogenesis and/or angiogenesis. The unfavorable degradation kinetics of β-TCP-based bone substitutes may result in an imbalance between resorption and osseous regeneration. Composite materials like Vitoss BA (Vitoss supplemented with 20 wt % 45S5-BG particles) might help to overcome these limitations. However, the influence of BG particles in Vitoss BA compared to unsupplemented Vitoss on osteogenesis, resorption behavior, and angiogenesis is not yet described. In this study, Vitoss and Vitoss BA scaffolds were seeded with human mesenchymal stromal cells before subcutaneous implantation in immunodeficient mice for 10 weeks. Scaffold resorption was monitored by micro-computed tomography, while osteoid formation and vascularization were assessed by histomorphometry and gene expression analysis. Whilst slightly more osteoid and improved angiogenesis were found in Vitoss BA, maturation of the osteoid was more advanced in Vitoss scaffolds. The volume of Vitoss implants decreased significantly, combined with a significantly increased presence of resorbing cells, whilst the volume remained stable in Vitoss BA scaffolds. Future studies should evaluate the interaction of 45S5-BG with resorbing cells and bone precursor cells in greater detail to improve the understanding and application of β-TCP/45S5-BG composite bone substitute materials.

In Vivo Degradation Behavior of Photo-Cross-Linkedstar-Poly(ε-caprolactone-co-d,l-lactide) Elastomers

2006 ◽  
Vol 7 (1) ◽  
pp. 365-372 ◽  
Author(s):  
Brian G. Amsden ◽  
M. Yat Tse ◽  
Norma D. Turner ◽  
Darryl K. Knight ◽  
Stephen C. Pang
Keyword(s):  
Degradation Behavior ◽  
In Vivo Degradation

scholarly journals Masticatory biomechanics in the rabbit: a multi-body dynamics analysis

2014 ◽  
Vol 11 (99) ◽  
pp. 20140564 ◽  
Author(s):  
Peter J. Watson ◽  
Flora Gröning ◽  
Neil Curtis ◽  
Laura C. Fitton ◽  
Anthony Herrel ◽  
...  
Keyword(s):  
Imaging Techniques ◽  
Three Dimensional ◽  
Muscle Fibres ◽  
Micro Computed Tomography ◽  
Body Dynamics ◽  
Masticatory System ◽  
Muscle Groups ◽  
Muscle Activations ◽  
Multi Body

Multi-body dynamics is a powerful engineering tool which is becoming increasingly popular for the simulation and analysis of skull biomechanics. This paper presents the first application of multi-body dynamics to analyse the biomechanics of the rabbit skull. A model has been constructed through the combination of manual dissection and three-dimensional imaging techniques (magnetic resonance imaging and micro-computed tomography). Individual muscles are represented with multiple layers, thus more accurately modelling muscle fibres with complex lines of action. Model validity was sought through comparing experimentally measured maximum incisor bite forces with those predicted by the model. Simulations of molar biting highlighted the ability of the masticatory system to alter recruitment of two muscle groups, in order to generate shearing or crushing movements. Molar shearing is capable of processing a food bolus in all three orthogonal directions, whereas molar crushing and incisor biting are predominately directed vertically. Simulations also show that the masticatory system is adapted to process foods through several cycles with low muscle activations, presumably in order to prevent rapidly fatiguing fast fibres during repeated chewing cycles. Our study demonstrates the usefulness of a validated multi-body dynamics model for investigating feeding biomechanics in the rabbit, and shows the potential for complementing and eventually reducing in vivo experiments.

scholarly journals Wear of Glass-ceramic-veneered Zirconia Posterior FPDS After 10 Years

2020 ◽  
Author(s):  
Ragai Edward Matta ◽  
Constantin Motel ◽  
Elena Kirchner ◽  
Simon Stelzer ◽  
Werner Adler ◽  
...  
Keyword(s):  
Glass Ceramic ◽  
Zirconium Oxide ◽  
Multiple Testing ◽  
Clinical Success ◽  
Three Dimensional ◽  
Rank Test ◽  
Minimal Distance ◽  
Significance Level ◽  
Volume Decrease

Abstract BackgroundThe abrasion behavior of various ceramics is rarely investigated, though it is relevant for the clinical success of such restorations. The aim of this in vivo study was to evaluate the wear of glass-ceramic-veneered zirconium oxide frameworks over a period of 10 years.MethodsThe abrasive behavior of the restorations was examined in a total of 15 patients after a period of 3, 5, and 10 years using plaster models, which were then subjected to a scanning process on the Atos II industrial scanner and digitized for three-dimensional evaluation of the abrasion by the corresponding software (ATOS Professional 7.6). The individual post-examination models were compared to the baseline model and deviations calculated in the sense of the largest, punctual loss of material in millimeters ("minimal distance"), the average abrasion in millimeters ("mean distance"), and the volume decrease in cubic millimeters ("integrated distance"). Statistical analyses were performed using the Wilcoxon sign rank test or mixed regression models. Multiple testing was considered by Benjamini-Hochberg correction. The significance level was set at 0.05.ResultsWe found steadily increasing wear of the ceramic. The average volume decrease was significant (P < 0.001) at 3 years and 10 years (-3.25 mm3 and − 8.11 mm3, respectively).ConclusionsDespite the increasing wear of the glass-ceramic-veneered zirconium oxide frameworks in this study, the use of this class of materials can be regarded as clinically acceptable.This study is registered in DRKS - German Clinical Trials Registerwith the Register number 00021743

scholarly journals Amorphous Calcium Magnesium Phosphate Nanocomposites with Superior Osteogenic Activity for Bone Regeneration

2021 ◽  
Author(s):  
Yingying Jiang ◽  
Shuo Tan ◽  
Jianping Hu ◽  
Xin Chen ◽  
Feng Chen ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Repair ◽  
Magnesium Phosphate ◽  
In Vivo Studies ◽  
Potential Candidate ◽  
Collagen Scaffolds ◽  
Edge Structure ◽  
X Ray ◽  
Calcium Magnesium

Abstract The seek of bioactive materials for promoting bone regeneration is a challenging and long-term task. Functionalization with inorganic metal ions or drug molecules are considered effective strategies to improve the bioactivity of various existing biomaterials. Herein, amorphous calcium magnesium phosphate (ACMP) nanoparticles and simvastatin (SIM)-loaded ACMP (ACMP/SIM) nanocomposites were developed via a simple coprecipitation strategy. The physiochemical property of ACMP/SIM were explored using transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), powder X-ray diffraction (XRD) and high performance liquid chromatograph (HPLC), and the role of Mg2+ in the formation of ACMP/SIM was revealed using X-ray absorption near-edge structure (XANES). After that, the transformation process of ACMP/SIM in simulated body fluid (SBF) was also tracked to simulate and explore the in vivo mineralization performance of materials. We find that ACMP/SIM releases ions of Ca2+, Mg2+ and PO43-, when it is immersed in SBF at 37 °C, and a phase transformation occured during which the initially amorphous ACMP turns into self-assembled hydroxyapatite (HAP). Furthermore, ACMP/SIM displays high cytocompatibility and promotes the proliferation and osteogenic differentiation of MC3T3-E1 cells. For the in vivo studies, lamellar ACMP/SIM/Collagen scaffolds with aligned pore structures were prepared and used to repair a rat defect model in calvaria. ACMP/SIM/Collagen scaffolds show a positive effect in promoting the regeneration of calvaria defect after 12 weeks. The bioactive ACMP/SIM nanocomposites are promising as bone repair materials. Considering the facile preparation process and superior in vitro/vivo bioactivity, the as-prepared ACMP/SIM would be a potential candidate for bone related biomedical applications.

Sign in / Sign up

Export Citation Format

Share Document