3D Bioprinting-Tunable Small-Diameter Blood Vessels with Biomimetic Biphasic Cell Layers

2020 ◽  
Vol 12 (41) ◽  
pp. 45904-45915
Author(s):  
Xuan Zhou ◽  
Margaret Nowicki ◽  
Hao Sun ◽  
Sung Yun Hann ◽  
Haitao Cui ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Small Diameter ◽  
3D Bioprinting ◽  
Cell Layers

scholarly journals A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting Technology

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1581 ◽  
Author(s):  
Yuanyuan Xu ◽  
Yingying Hu ◽  
Changyong Liu ◽  
Hongyi Yao ◽  
Boxun Liu ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Structural Integrity ◽  
Vascular System ◽  
Small Diameter ◽  
Dermal Fibroblasts ◽  
Pluronic F127 ◽  
3D Bioprinting ◽  
Novel Strategy ◽  
Blood Vessel Substitutes

In this work, a novel strategy was developed to fabricate prevascularized cell-layer blood vessels in thick tissues and small-diameter blood vessel substitutes using three-dimensional (3D) bioprinting technology. These thick vascularized tissues were comprised of cells, a decellularized extracellular matrix (dECM), and a vasculature of multilevel sizes and multibranch architectures. Pluronic F127 (PF 127) was used as a sacrificial material for the formation of the vasculature through a multi-nozzle 3D bioprinting system. After printing, Pluronic F127 was removed to obtain multilevel hollow channels for the attachment of human umbilical vein endothelial cells (HUVECs). To reconstruct functional small-diameter blood vessel substitutes, a supporting scaffold (SE1700) with a double-layer circular structure was first bioprinted. Human aortic vascular smooth muscle cells (HA-VSMCs), HUVECs, and human dermal fibroblasts–neonatal (HDF-n) were separately used to form the media, intima, and adventitia through perfusion into the corresponding location of the supporting scaffold. In particular, the dECM was used as the matrix of the small-diameter blood vessel substitutes. After culture in vitro for 48 h, fluorescent images revealed that cells maintained their viability and that the samples maintained structural integrity. In addition, we analyzed the mechanical properties of the printed scaffold and found that its elastic modulus approximated that of the natural aorta. These findings demonstrate the feasibility of fabricating different kinds of vessels to imitate the structure and function of the human vascular system using 3D bioprinting technology.

Scaffolds in tissue engineering of blood vessels

2010 ◽  
Vol 88 (9) ◽  
pp. 855-873 ◽  
Author(s):  
Divya Pankajakshan ◽  
Devendra K. Agrawal
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Biological Scaffolds ◽  
Hemodynamic Forces ◽  
Biodegradable Scaffolds

Tissue engineering of small diameter (<5 mm) blood vessels is a promising approach for developing viable alternatives to autologous vascular grafts. It involves in vitro seeding of cells onto a scaffold on which the cells attach, proliferate, and differentiate while secreting the components of extracellular matrix that are required for creating the tissue. The scaffold should provide the initial requisite mechanical strength to withstand in vivo hemodynamic forces until vascular smooth muscle cells and fibroblasts reinforce the extracellular matrix of the vessel wall. Hence, the choice of scaffold is crucial for providing guidance cues to the cells to behave in the required manner to produce tissues and organs of the desired shape and size. Several types of scaffolds have been used for the reconstruction of blood vessels. They can be broadly classified as biological scaffolds, decellularized matrices, and polymeric biodegradable scaffolds. This review focuses on the different types of scaffolds that have been designed, developed, and tested for tissue engineering of blood vessels, including use of stem cells in vascular tissue engineering.

Abnormal skin development in pupoid foetus (pf/pf) mutant mice

Development ◽  
1985 ◽  
Vol 87 (1) ◽  
pp. 47-64
Author(s):  
Chris Fisher ◽  
Edward J. Kollar
Keyword(s):  
Blood Vessels ◽  
Schwann Cells ◽  
Cell Population ◽  
Epidermal Cell ◽  
Basal Lamina ◽  
Mutant Mice ◽  
Nerve Fibres ◽  
Skin Development ◽  
Cell Layers

At 13 days of development the epidermis of mice homozygous for the pupoid foetus (pf/pf) mutation varies in thickness between one and ten cell layers. By 16 days of development cells from the dermis have invaded the epidermis and may be found throughout the epidermis and on its surface. Among these cells are nerve fibres and Schwann cells as well as other unidentified cells. Antibodies directed against fibronectin bind to these abnormal groups of cells in the mutant epidermis and on its surface. A basal lamina, as determined by ultrastructure and by the immuno-fluorescent localization of laminin, was always found at the interface of the mutant epidermis and the invading cell population. By 19 days of development the mutant epidermis is thickened and is permeated by a network of cells including nerve fibres, Schwann cells, blood vessels, and collagen and fibronectin-secreting cells. A basal lamina always separates these groups of invading cells from the epidermal cell population.

3D Coculture of Human Endothelial Cells and Myofibroblasts for Vascular Tissue Engineering

2007 ◽  
Author(s):  
Rolf A. A. Pullens ◽  
Maria Stekelenburg ◽  
Carlijn V. C. Bouten ◽  
Frank P. T. Baaijens ◽  
Mark J. Post
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Artery Bypass ◽  
Small Diameter ◽  
Life Time ◽  
Mechanical Conditioning ◽  
Tissue Properties ◽  
Vein Grafts ◽  
Limited Life

Cardiovascular disease is still the number one cause of death in the industrialized world. Diseased small diameter blood vessels are frequently replaced by native grafts. However, these vessels have a limited life time [1], for example the patency at 10 year after coronary artery bypass grafting of saphenous vein grafts is 57% [2]. Tissue engineering (TE) of small diameter blood vessels seems a promising approach to overcome these shortcomings or address the increasing need for substitutes during follow up surgery. Mechanical conditioning of myofibroblast (MFs) seeded constructs appears to be beneficial for functional tissue properties, such as cell proliferation, ECM production and mechanical strength [3,4]. Without a functional endothelial cell (ECs) layer however, patency may be compromised by thrombogenecity. Construction of an EC layer might on the other hand affect the tissue composition during culture, as was shown for bovine ECs, which influenced proliferation and ECM production of smooth muscle cells [5].

Biomechanical Properties of Self-Assembly Tissue Engineered Blood Vessels: Insights Into Assembly Techniques

2010 ◽  
Author(s):  
Michael T. Zaucha ◽  
Rudolph Gleason
Keyword(s):  
Coronary Artery Disease ◽  
Blood Vessels ◽  
Self Assembly ◽  
Small Diameter ◽  
Biomechanical Properties ◽  
Industrialized Nations ◽  
Tissue Engineered Blood Vessels ◽  
Artery Disease ◽  
Thrombogenic Potential ◽  
Synthetic Grafts

Coronary artery disease remains to be the leading cause of morbidity and mortality in industrialized nations. Current treatments for small diameter grafts are limited by the availability of suitable autologous vessels and high thrombogenic potential of synthetic grafts. There is a clinical need to development of tissue engineered blood vessels (TEBV) suitable for vascular by pass grafting.

Development of Small-Diameter Vascular Grafts Through Decellularization of Human Blood Vessels

2017 ◽  
Vol 7 (2) ◽  
pp. 101-110 ◽  
Author(s):  
Andrea Porzionato ◽  
Maria Martina Sfriso ◽  
Alex Pontini ◽  
Veronica Macchi ◽  
Maria Ida Buompensiere ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Human Blood ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Small Diameter Vascular Grafts

scholarly journals Feasibility and Safety of Unzipping Small Diameter Stents in the Blood Vessels of Piglets

2016 ◽  
Vol 9 (11) ◽  
pp. 1138-1149 ◽  
Author(s):  
Shyam K. Sathanandam ◽  
T.K. Susheel Kumar ◽  
Deepthi Hoskoppal ◽  
Lauren M. Haddad ◽  
Saradha Subramanian ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Small Diameter

Fabrication and Preliminary Study of a Prototype Bi-Layered Small Diameter Vascular Prosthesis Composed of Nano-Fiber and Silk Fiber

2013 ◽  
Vol 843 ◽  
pp. 66-69 ◽  
Author(s):  
Hui Jing Zhao ◽  
Guo Li Zhou ◽  
Zhi Qing Yuan
Keyword(s):  
Mechanical Properties ◽  
Blood Vessels ◽  
Outer Layer ◽  
Small Diameter ◽  
Peak Stress ◽  
Silk Fiber ◽  
Content Increase ◽  
Peak Strain ◽  
Tissue Engineering Scaffolds ◽  
Vascular Prostheses

Biomaterials used for vascular prostheses should possess certain strength that can keep the normal blood fluidity, as well as certain flexibility and elasticity that can resist blood pulsation pressure. In order to fabricate small diameter vascular prostheses (SDVP) that possess matchable mechanical properties with natural blood vessels, a bi-layered tubular structure composed of electrospinning blended nanofiber and silk fiber was designed and prepared in this study. The inner layer of the structure, prepared through electrospinning, was composed of Poly (L-lactide-co-ε-caprolactone) (PLCL) and silk fibroin (SF) blended nanofibers. Braided silk tube was used as the outer layer of the structure. Morphological, structural and mechanical properties including peak stress, peak strain, and Youngs modulus of the prototype bi-layered SDVP were characterized initially. Results showed that the diameter range of the blended nanofiber was between 100 and 900 nm, and the fiber diameter increased with the content increase of PLCL. Through blending PLCL together with SF, peak stress and peak strain of the electrospun inner layer were improved, and that of the Youngs modulus decreased. Meanwhile, the outer layer of SDVP was stronger and had higher Youngs modulus. Those mechanical performances of the prototype bi-layered SDVP fabricated in this study are similar to natural blood vessels, which provide a promising biomaterial that could be applied on tubular tissue engineering scaffolds.

Electrospun polylactide/silk fibroin-gelatin composite tubular scaffolds for small-diameter tissue engineering blood vessels

2009 ◽  
Vol 113 (4) ◽  
pp. 2675-2682 ◽  
Author(s):  
Shudong Wang ◽  
Youzhu Zhang ◽  
Guibo Yin ◽  
Hongwei Wang ◽  
Zhihui Dong
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Silk Fibroin ◽  
Small Diameter

Theory and Experiments for Mechanically-Induced Remodeling of Tissue Engineered Blood Vessels

2008 ◽  
Vol 57 ◽  
pp. 226-234 ◽  
Author(s):  
Rudolph L. Gleason ◽  
William Wan
Keyword(s):  
Mechanical Properties ◽  
Blood Vessels ◽  
Laser Scanning ◽  
Biomechanical Testing ◽  
Small Diameter ◽  
Mechanical Stimuli ◽  
Two Photon ◽  
Constrained Mixture ◽  
Tissue Engineered Blood Vessels ◽  
Luminal Flow

There is a great unmet clinical need to develop small diameter tissue engineered blood vessels (TEBV) with low thrombogenicity and immune response and suitable mechanical properties. In this paper we describe experimental and computational frameworks to characterize the use of mechanical stimuli to improve the mechanical properties of TEBVs. We model the TEBV as a constrained mixture and track the production, degradation, mechanical state, and organization of each structural constituent. Specifically, we assume that individual load bearing constituents can co-exist within each neighborhood and, although they are constrained to deform together, each constituent within this neighborhood may have different natural (i.e., stress-free) configurations. Motivated by this theoretical framework, we have designed a bioreactor and biomechanical testing device for TEBVs. This device is designed to provide precise and independent control of mean and cyclic luminal flow rate, transmural pressure, and axial load over weeks and months in culture and perform intermittent biaxial biomechanical tests. This device also fits under a two-photon laser scanning microscope for 3-dimenstional imaging of the content and organization of cells and matrix constituents. These data directly support our theoretical model.

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