scholarly journals Identification of the Genetic Basis of Response to de-Acclimation in Winter Barley

2021 ◽  
Vol 22 (3) ◽  
pp. 1057
Author(s):  
Magdalena Wójcik-Jagła ◽  
Agata Daszkowska-Golec ◽  
Anna Fiust ◽  
Przemysław Kopeć ◽  
Marcin Rapacz
Keyword(s):  
Enzyme Activity ◽  
Stress Response ◽  
Genetic Basis ◽  
Differential Expression Analysis ◽  
Winter Barley ◽  
Developmental Changes ◽  
Molecular Regulation ◽  
Quantitative Real Time Pcr ◽  
Breeding Lines ◽  
Functionally Diverse

Mechanisms involved in the de-acclimation of herbaceous plants caused by warm periods during winter are poorly understood. This study identifies the genes associated with this mechanism in winter barley. Seedlings of eight accessions (four tolerant and four susceptible to de-acclimation cultivars and advanced breeding lines) were cold acclimated for three weeks and de-acclimated at 12 °C/5 °C (day/night) for one week. We performed differential expression analysis using RNA sequencing. In addition, reverse-transcription quantitative real-time PCR and enzyme activity analyses were used to investigate changes in the expression of selected genes. The number of transcripts with accumulation level changed in opposite directions during acclimation and de-acclimation was much lower than the number of transcripts with level changed exclusively during one of these processes. The de-acclimation-susceptible accessions showed changes in the expression of a higher number of functionally diverse genes during de-acclimation. Transcripts associated with stress response, especially oxidoreductases, were the most abundant in this group. The results provide novel evidence for the distinct molecular regulation of cold acclimation and de-acclimation. Upregulation of genes controlling developmental changes, typical for spring de-acclimation, was not observed during mid-winter de-acclimation. Mid-winter de-acclimation seems to be perceived as an opportunity to regenerate after stress. Unfortunately, it is competitive to remain in the cold-acclimated state. This study shows that the response to mid-winter de-acclimation is far more expansive in de-acclimation-susceptible cultivars, suggesting that a reduced response to the rising temperature is crucial for de-acclimation tolerance.

scholarly journals Transcriptome-based identification and characterization of genes responding to imidacloprid in Myzus persicae

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jianyu Meng ◽  
Xingjiang Chen ◽  
Changyu Zhang
Keyword(s):  
Genetic Basis ◽  
Expression Profiles ◽  
Control Measure ◽  
Effective Control ◽  
Agricultural Pest ◽  
Functional Classification ◽  
Quantitative Real Time Pcr ◽  
Underlying Mechanisms ◽  
Identification And Characterization

Abstract Myzus persicae is a serious and widespread agricultural pest, against which, imidacloprid remains an effective control measure. However, recent reports indicate that this aphid has evolved and developed resistance to imidacloprid. This study aimed to elucidate the underlying mechanisms and genetic basis of this resistance by conducting comparative transcriptomics studies on both imidacloprid-resistant (IR) and imidacloprid-susceptible (IS) M. persicae. The comparative analysis identified 252 differentially expressed genes (DEGs) among the IR and IS M. persicae transcriptomes. These candidate genes included 160 and 92 genes that were down- and up-regulated, respectively, in the imidacloprid-resistant strain. Using functional classification in the GO and KEGG databases, 187 DEGs were assigned to 303 functional subcategories and 100 DEGs were classified into 45 pathway groups. Moreover, several genes were associated with known insecticide targets, cuticle, metabolic processes, and oxidative phosphorylation. Quantitative real-time PCR of 10 DEGs confirmed the trends observed in the RNA sequencing expression profiles. These findings provide a valuable basis for further investigation into the complicated mechanisms of imidacloprid resistance in M. persicae.

scholarly journals Aspergillus flavus NRRL 35739, a Poor Biocontrol Agent, May Have Increased Relative Expression of Stress Response Genes

2019 ◽  
Vol 5 (2) ◽  
pp. 53 ◽  
Author(s):  
Kayla K. Pennerman ◽  
Guohua Yin ◽  
Joan W. Bennett ◽  
Sui-Sheng T. Hua
Keyword(s):  
Stress Response ◽  
Aspergillus Flavus ◽  
Genetic Basis ◽  
Biocontrol Agent ◽  
Success Rates ◽  
Lower Stress ◽  
Relative Expression ◽  
Stress Response Genes ◽  
Variable Success ◽  
Aflatoxin Accumulation

Biocontrol of the mycotoxin aflatoxin utilizes non-aflatoxigenic strains of Aspergillus flavus, which have variable success rates as biocontrol agents. One non-aflatoxigenic strain, NRRL 35739, is a notably poor biocontrol agent. Its growth in artificial cultures and on peanut kernels was found to be slower than that of two aflatoxigenic strains, and NRRL 35739 exhibited less sporulation when grown on peanuts. The non-aflatoxigenic strain did not greatly prevent aflatoxin accumulation. Comparison of the transcriptomes of aflatoxigenic and non-aflatoxigenic A. flavus strains AF36, AF70, NRRL 3357, NRRL 35739, and WRRL 1519 indicated that strain NRRL 35739 had increased relative expression of six heat shock and stress response proteins, with the genes having relative read counts in NRRL 35739 that were 25 to 410 times more than in the other four strains. These preliminary findings tracked with current thought that aflatoxin biocontrol efficacy is related to the ability of a non-aflatoxigenic strain to out-compete aflatoxigenic ones. The slower growth of NRRL 35739 might be due to lower stress tolerance or overexpression of stress response(s). Further study of NRRL 35739 is needed to refine our understanding of the genetic basis of competitiveness among A. flavus strains.

scholarly journals Occurrence of dwarf virus of winter wheat and barley in several regions of Slovakiaduring the growing seasons 2001–2004

2011 ◽  
Vol 52 (No. 9) ◽  
pp. 392-401 ◽  
Author(s):  
N. Bukvayová ◽  
M. Henselová ◽  
V. Vajcíková ◽  
T. Kormanová
Keyword(s):  
Winter Wheat ◽  
Viral Infection ◽  
Large Scale ◽  
Winter Barley ◽  
Dwarf Virus ◽  
Breeding Lines ◽  
Growing Seasons

The aim of the study was to monitor the incidence and to detect the presence of viruses of yellow dwarfness in barley (BYDV-PAV, BYDV-RMV), of yellow dwarfness in cereals (CYDV-RPV) and dwarfness in wheat (WDV) in stands of winter wheat and winter barley in Slovakia. During the period 2001–2004 a total of 292 samples coming from 150 localities were analyzed. This involved 190 samples of winter wheat (39 varieties and 13 breeding lines) and 102 samples of winter barley (17 varieties and 7 breeding lines). The detection of viruses was carried out with the aid of the method DAS and TAS ELISA. During the years surveyed, the occurrence of the various viruses differed. In 2001, the most represented virus proved to be the WDV (68%); in 2002, it was the strain PAV of the virus BYDV (93%); in 2003, the most numerous were the virus WDV (71%) and the strain PAV of virus BYDV (67%). Similarly, in 2004, two viruses were represented about evenly, WDV and BYDV-PAV (75%). The more frequent of the two species was the virus BYDV, with the strain BYDV-PAV predominating. The intensity of viral infection of stand cereals differed during the experimental years, being highest in 2002 when the blight occurred both locally and also on a large-scale. The highest frequency of the disease was in Western and Eastern Slovakia.

scholarly journals Genetic analysis of the molecular regulation of electric fields-guided glia migration

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Li Yao ◽  
Teresa Shippy ◽  
Yongchao Li
Keyword(s):  
Cell Migration ◽  
Rna Sequencing ◽  
Electric Fields ◽  
Differential Expression Analysis ◽  
Cell Types ◽  
Molecular Regulation ◽  
Sequencing Data ◽  
Oligodendrocyte Precursor ◽  
Go Terms ◽  
Developing Nervous System

Abstract In a developing nervous system, endogenous electric field (EF) influence embryonic growth. We reported the EF-directed migration of both rat Schwann cells (SCs) and oligodendrocyte precursor cells (OPCs) and explored the molecular mechanism using RNA-sequencing assay. However, previous studies revealed the differentially expressed genes (DEGs) associated with EF-guided migration of SCs or OPCs alone. In this study, we performed joint differential expression analysis on the RNA-sequencing data from both cell types. We report a number of significantly enriched gene ontology (GO) terms that are related to the cytoskeleton, cell adhesion, and cell migration. Of the DEGs associated with these terms, nine up-regulated DEGs and 32 down-regulated DEGs showed the same direction of effect in both SCs and OPCs stimulated with EFs, while the remaining DEGs responded differently. Thus, our study reveals the similarities and differences in gene expression and cell migration regulation of different glial cell types in response to EF stimulation.

Quantitative real time PCR of deoxycytidine kinase mRNA by Light Cycler PCR in relation to enzyme activity and gemcitabine sensitivity

2004 ◽  
Vol 213 (2) ◽  
pp. 173-179 ◽  
Author(s):  
Jennifer Sigmond ◽  
Judith R. Kroep ◽  
Willem Loves ◽  
Giovanni Codacci-Pisanelli ◽  
Godefridus J. Peters
Keyword(s):  
Enzyme Activity ◽  
Real Time ◽  
Real Time Pcr ◽  
Deoxycytidine Kinase ◽  
Quantitative Real Time Pcr ◽  
Light Cycler

scholarly journals Ontogenesis of the HPI axis and molecular regulation of the cortisol stress response during early development in Dicentrarchus labrax.

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
A. Tsalafouta ◽  
N. Papandroulakis ◽  
M. Gorissen ◽  
P. Katharios ◽  
G. Flik ◽  
...  
Keyword(s):  
Stress Response ◽  
Early Development ◽  
Dicentrarchus Labrax ◽  
Molecular Regulation ◽  
Hpi Axis

Vernalization responses in narrow-leafed lupin (Lupinus angustifolius) genotypes

1995 ◽  
Vol 46 (5) ◽  
pp. 1011 ◽  
Author(s):  
KF Landers
Keyword(s):  
Genetic Basis ◽  
Major Gene ◽  
Mutant Gene ◽  
Major Effect ◽  
Lupinus Angustifolius ◽  
Vernalization Response ◽  
Essential Requirement ◽  
Breeding Lines ◽  
Sowing Dates ◽  
Absolute Requirement

Three experiments were conducted to characterize vernalization response in 13 diverse narrowleafed lupin (Lupinus angustifolius) genotypes, and to identify the genetic basis of differences in vernalization response. The aim was to better understand how flowering time may be manipulated in lupin breeding. The genotypes consisted of breeding lines with parents of wild origin, plus selected commercial varieties. Treatments included response to different periods of vernalization and response to different sowing dates. Most of the genotypes required vernalization for flowering. There were three types of response to vernalization observed; an absolute requirement, a reduced response, in which vernalization did not appear to be essential for flowering, and no response in lines carrying the natural mutant gene Ku (Gladstones and Hill 1969). In genotypes with an absolute requirement for vernalization, the period of vernalization at 5�C required to ensure flowering varied between 2 and 4 weeks, and flowering was hastened by increasing periods of vernalization. When vernalization was marginally inadequate, abnormal inflorescences formed. An apparent thermosensitive response, in which vernalization hastened flowering but did not appear to be essential, occurred in cv. Wandoo, which carries the gene �efl�. This response could also possibly be explained not by the lack of an essential requirement for vernalization, but by an ability of the cultivar to respond to vernalization at fairly high temperatures, around 16�C. Crossing studies identified a major gene the same as or allelic to �efl� in one genotype, but no other single genes with major effect on vernalization response were detected in genotypes of wild origin.

The potential of new genetic technologies in selecting for stress resistance in pigs

2005 ◽  
Vol 45 (8) ◽  
pp. 775
Author(s):  
C. A. Kerr ◽  
B. M. Hines
Keyword(s):  
Gene Expression ◽  
Stress Response ◽  
Stress Resistance ◽  
Genetic Basis ◽  
Molecular Mechanisms ◽  
Negative Impact ◽  
Experimental Models ◽  
Genetic Technologies ◽  
On Farm ◽  
Commercial Environment

This paper examines the potential for breeding stress resistance in pigs through an understanding of the physiology of the stress response and its associated genetic basis. Pigs reared in commercial units can encounter numerous concurrent stressors that can have a negative impact on performance and welfare. Stress induces physiological and behavioural responses that are multidimensional, consisting of a complex neuroendocrine and immune signalling milieu. Some stress-related genetic parameters have been identified using conventional genetic approaches applied in experimental models. However, these traits do not capture the complexity of the stress response. As a result, the molecular mechanisms underlying the variation associated with stress resistance in pigs in a commercial environment is poorly understood. Gene expression profiling is a powerful tool that can be applied to systematically elucidate stress response pathways and networks. Consequently, gene expression technologies have been applied to identify some putative stress-regulated genes. Further application of these and more traditional technologies will aid in elucidating stress resistance using gene expression as a measure of phenotypic variation at a molecular level. It is envisaged that in the future, tools for selecting for stress resistance could eventually be applied on-farm to enhance production, health and welfare status.

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