scholarly journals Bordetella holmesii Lipopolysaccharide Hide and Seek Game with Pertussis: Structural Analysis of the O-Specific Polysaccharide and the Core Oligosaccharide of the Type Strain ATCC 51541

2020 ◽  
Vol 21 (17) ◽  
pp. 6433
Author(s):  
Karolina Ucieklak ◽  
Sabina Koj ◽  
Tomasz Niedziela
Keyword(s):  
Whooping Cough ◽  
13C Nmr Spectroscopy ◽  
Structural Features ◽  
The Novel ◽  
Core Oligosaccharide ◽  
The Core ◽  
Sds Page ◽  
Specific Polysaccharide ◽  
First Time ◽  
Hydrolysis Of

Whooping cough is a highly contagious disease caused predominantly by Bordetella pertussis, but it also comprises of a pertussis-like illness caused by B. holmesii. The virulence factors of B. holmesii and their role in the pathogenesis remain unknown. Lipopolysaccharide is the main surface antigen of all Bordetellae. Data on the structural features of the lipopolysaccharide (LPS) of B. holmesii are scarce. The poly- and oligosaccharide components released by mild acidic hydrolysis of the LPS were separated and investigated by 1H and 13C NMR spectroscopy, mass spectrometry, and chemical methods. The structures of the O-specific polysaccharide and the core oligosaccharide of B. holmesii ATCC 51541 have been identified for the first time. The novel pentasaccharide repeating unit of the B. holmesii O-specific polysaccharide has the following structure: {→2)-α-l-Rhap-(1→6)-α-d-Glcp-(1→4)-[β-d-GlcpNAc-(1→3]-α-d-Galp-(1→3)-α-d-GlcpNAc-(1→}n. The SDS-PAGE and serological cross-reactivities of the B. holmesii LPS suggested the similarity between the core oligosaccharides of B. holmesii ATCC 51541 and B. pertussis strain 606. The main oligosaccharide fraction contained a nonasaccharide. The comparative analysis of the NMR spectra of B. holmesii core oligosaccharide fraction with this of the B. pertussis strain 606 indicated that the investigated core oligosaccharides were identical.

scholarly journals Expression of the Novel Hepatitis C Virus Core+1/ARF Protein in the Context of JFH1-Based Replicons

2015 ◽  
Vol 89 (9) ◽  
pp. 5164-5170 ◽  
Author(s):  
Ioly Kotta-Loizou ◽  
Ioannis Karakasiliotis ◽  
Niki Vassilaki ◽  
Panagiotis Sakellariou ◽  
Ralf Bartenschlager ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Gene ◽  
Open Reading Frame ◽  
The Novel ◽  
Reading Frame ◽  
The Core ◽  
Virus Core ◽  
First Time ◽  
Arf Protein

Hepatitis C virus contains a second open reading frame within the core gene, designated core+1/ARF. Here we demonstrate for the first time expression of core+1/ARF protein in the context of a bicistronic JFH1-based replicon and report the production of two isoforms, core+1/L (long) and core+1/S (short), with different kinetics.

scholarly journals Coffee carbohydrates

2006 ◽  
Vol 18 (1) ◽  
pp. 165-174 ◽  
Author(s):  
Robert Redgwell ◽  
Monica Fischer
Keyword(s):  
Cell Wall ◽  
Potential Role ◽  
Structural Features ◽  
Coffee Bean ◽  
Cell Wall Polysaccharides ◽  
Pectic Polysaccharides ◽  
Specific Polysaccharide ◽  
Chemical Studies ◽  
First Time ◽  
Detailed Chemical

This review summarises recent advances in the chemistry, physiology and molecular properties of coffee carbohydrates with a particular focus on the cell wall polysaccharides. The results of detailed chemical studies have demonstrated novel structural features of both the galactomannans and the arabinogalactan polysaccharides of the green and roasted coffee bean. For the first time immunological probes based on monoclonal antibodies for specific polysaccharide epitopes were used to reveal the patterns of distribution of the galactomannans, arabinogalactans and pectic polysaccharides in the coffee bean cell wall. Finally, the results of physiological and molecular studies are presented which emphasise the growing awareness of the potential role the metabolic status of the green bean may play in final coffee beverage quality.

scholarly journals Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL

Marine Drugs ◽  
2021 ◽  
Vol 19 (9) ◽  
pp. 508
Author(s):  
Elena N. Sigida ◽  
Ibrahim M. Ibrahim ◽  
Maxim S. Kokoulin ◽  
Hussein H. Abulreesh ◽  
Khaled Elbanna ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
13C Nmr Spectroscopy ◽  
Halophilic Bacterium ◽  
Mild Acid Hydrolysis ◽  
1H And 13C Nmr ◽  
Specific Polysaccharide ◽  
Lake Qarun ◽  
Phenol Water ◽  
Hydrolysis Of ◽  
Mild Acid

The moderately halophilic strain Salinivibrio sp. EG9S8QL was isolated among 11 halophilic strains from saline mud (Emisal Salt Company, Lake Qarun, Fayoum, Egypt). The lipopolysaccharide was extracted from dried cells of Salinivibrio sp. EG9S8QL by the phenol–water procedure. The OPS was obtained by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with 1H and 13C NMR spectroscopy, including 1H,1H COSY, TOCSY, ROESY, 1H,13C HSQC, and HMBC experiments. The OPS was found to be composed of linear tetrasaccharide repeating units of the following structure: →2)-β-Manp4Lac-(1→3)-α-ManpNAc-(1→3)-β-Rhap-(1→4)-α-GlcpNAc-(1→, where Manp4Lac is 4-O-[1-carboxyethyl]mannose.

scholarly journals OBJECTIVATION OF THE INTROVERT TYPE OF A PERSONALITY IN THE NOVEL"TICKET TO THE STARS" BY V. AKSYONOV

2019 ◽  
Vol 8 (7) ◽  
Author(s):  
Liliya F. Bormusova ◽  
Dinara I. Rakhimov ◽  
Nailya N. Fattakhova
Keyword(s):  
Psychological Type ◽  
Professional Activity ◽  
The Novel ◽  
Main Character ◽  
Psychological Types ◽  
The Core ◽  
Individual Traits ◽  
Methods Of Synthesis ◽  
The Individual ◽  
First Time

The current research is executed in the mainstream of psycholinguistics within which the theory of psychological types has been developing in the last decades. The analysis of an introspective psychological type is carried out in the article on the basis of an art discourse viewing which we are guided by the theory of the identity of K.G. Jung. On the material of the story by V.P. Aksyonov "Ticket to the stars" the introspective type of the personality is being investigated for the first time; it is represented by the personage of the graduate student Victor, the elder brother of the main character of the story – of Dimka. In general, being stereotypic, the analyzed character has a number of the individual traits latently pointing to his professional activity. For the description of structure of the core of a psychological type the typified and individual signs are revealed; the share of these signs in the formation of the studied psychological type is defined. The analysis was carried out with the application of the methods of synthesis and generalization.

The novel structure of the core oligosaccharide backbone of the lipopolysaccharide from the Plesiomonas shigelloides strain CNCTC 80/89 (serotype O13)

2013 ◽  
Vol 380 ◽  
pp. 45-50 ◽  
Author(s):  
Marta Kaszowska ◽  
Wojciech Jachymek ◽  
Tomasz Niedziela ◽  
Sabina Koj ◽  
Lennart Kenne ◽  
...  
Keyword(s):  
The Novel ◽  
Core Oligosaccharide ◽  
Plesiomonas Shigelloides ◽  
The Core ◽  
Novel Structure

scholarly journals A Unique Sugar l-Perosamine (4-Amino-4,6-dideoxy-l-mannose) Is a Compound Building Two O-Chain Polysaccharides in the Lipopolysaccharide of Aeromonas hydrophila Strain JCM 3968, Serogroup O6

Marine Drugs ◽  
2019 ◽  
Vol 17 (5) ◽  
pp. 254 ◽  
Author(s):  
Katarzyna Dworaczek ◽  
Maria Kurzylewska ◽  
Magdalena A. Karaś ◽  
Monika Janczarek ◽  
Agnieszka Pękala-Safińska ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Aeromonas Hydrophila ◽  
Lipid A ◽  
Gel Permeation Chromatography ◽  
13C Nmr Spectroscopy ◽  
Bacterial Strains ◽  
Core Oligosaccharide ◽  
O Antigen ◽  
Specific Polysaccharide ◽  
Exposed Part

Lipopolysaccharide (LPS) is the major glycolipid and virulence factor of Gram-negative bacteria, including Aeromonas spp. The O-specific polysaccharide (O-PS, O-chain, O-antigen), i.e., the surface-exposed part of LPS, which is a hetero- or homopolysaccharide, determines the serospecificity of bacterial strains. Here, chemical analyses, mass spectrometry, and 1H and 13C NMR spectroscopy techniques were employed to study the O-PS of Aeromonas hydrophila strain JCM 3968, serogroup O6. MALDI-TOF mass spectrometry revealed that the LPS of A. hydrophila JCM 3968 has a hexaacylated lipid A with conserved architecture of the backbone and a core oligosaccharide composed of Hep6Hex1HexN1HexNAc1Kdo1P1. To liberate the O-antigen, LPS was subjected to mild acid hydrolysis followed by gel-permeation-chromatography and revealed two O-polysaccharides that were found to contain a unique sugar 4-amino-4,6-dideoxy-l-mannose (N-acetyl-l-perosamine, l-Rhap4NAc), which may further determine the specificity of the serogroup. The first O-polysaccharide (O-PS1) was built up of trisaccharide repeating units composed of one α-d-GalpNAc and two α-l-Rhap4NAc residues, whereas the other one, O-PS2, is an α1→2 linked homopolymer of l-Rhap4NAc. The following structures of the O-polysaccharides were established: O-PS1 →3)-α-l-Rhap4NAc-(1→4)-α-d-GalpNAc-(1→3)-α-l-Rhap4NAc-(1→ O-PS2 →2)-α-l-Rhap4NAc-(1→ The present paper is the first work that reveals the occurrence of perosamine in the l-configuration as a component of bacterial O-chain polysaccharides.

Untimely Development, Ugly History:A Drama in Muslinand the Rejection of National-Historical Time

2013 ◽  
Vol 3 (2) ◽  
pp. 161-183
Author(s):  
Mary L. Mullen
Keyword(s):  
Everyday Life ◽  
The State ◽  
The Political ◽  
Nation State ◽  
The Novel ◽  
Historical Time ◽  
Political Processes ◽  
The Core

This article considers the politics and aesthetics of the colonial Bildungsroman by reading George Moore's often-overlooked novel A Drama in Muslin (1886). It argues that the colonial Bildungsroman does not simply register difference from the metropolitan novel of development or express tension between the core and periphery, as Jed Esty suggests, but rather can imagine a heterogeneous historical time that does not find its end in the nation-state. A Drama in Muslin combines naturalist and realist modes, and moves between Ireland and England to construct a form of untimely development that emphasises political processes (dissent, negotiation) rather than political forms (the state, the nation). Ultimately, the messy, discordant history represented in the novel shows the political potential of anachronism as it celebrates the untimeliness of everyday life.

Buried Narratives: Exhuming the Mother's Story in Oliver Twist

2018 ◽  
Vol 8 (3) ◽  
pp. 247-266
Author(s):  
Michelle L. Wilson
Keyword(s):  
Social Change ◽  
Maternal Inheritance ◽  
The Novel ◽  
Poor Law ◽  
The Family ◽  
The Law ◽  
First Time ◽  
Critical Consideration

Initially, Oliver Twist (1839) might seem representative of the archetypal male social plot, following an orphan and finding him a place by discovering the father and settling the boy within his inheritance. But Agnes Fleming haunts this narrative, undoing its neat, linear transmission. This reconsideration of maternal inheritance and plot in the novel occurs against the backdrop of legal and social change. I extend the critical consideration of the novel's relationship to the New Poor Law by thinking about its reflection on the bastardy clauses. And here, of course, is where the mother enters. Under the bastardy clauses, the responsibility for economic maintenance of bastard children was, for the first time, legally assigned to the mother, relieving the father of any and all obligation. Oliver Twist manages to critique the bastardy clauses for their release of the father, while simultaneously embracing the placement of the mother at the head of the family line. Both Oliver and the novel thus suggest that it is the mother's story that matters, her name through which we find our own. And by containing both plots – that of the father and the mother – Oliver Twist reveals the violence implicit in traditional modes of inheritance in the novel and under the law.

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