Structural Analysis of the Core Oligosaccharide and the O-Specific Polysaccharide from thePlesiomonas shigelloidesO33:H3 (Strain CNCTC 34/89) Lipopolysaccharide

2013 ◽  
Vol 2014 (6) ◽  
pp. 1241-1252 ◽  
Author(s):  
Gustav Nestor ◽  
Jolanta Lukasiewicz ◽  
Corine Sandström
2005 ◽  
Vol 340 (6) ◽  
pp. 1253-1257 ◽  
Author(s):  
Frank St. Michael ◽  
Jianjun Li ◽  
Andrew D. Cox

2020 ◽  
Vol 21 (17) ◽  
pp. 6433
Author(s):  
Karolina Ucieklak ◽  
Sabina Koj ◽  
Tomasz Niedziela

Whooping cough is a highly contagious disease caused predominantly by Bordetella pertussis, but it also comprises of a pertussis-like illness caused by B. holmesii. The virulence factors of B. holmesii and their role in the pathogenesis remain unknown. Lipopolysaccharide is the main surface antigen of all Bordetellae. Data on the structural features of the lipopolysaccharide (LPS) of B. holmesii are scarce. The poly- and oligosaccharide components released by mild acidic hydrolysis of the LPS were separated and investigated by 1H and 13C NMR spectroscopy, mass spectrometry, and chemical methods. The structures of the O-specific polysaccharide and the core oligosaccharide of B. holmesii ATCC 51541 have been identified for the first time. The novel pentasaccharide repeating unit of the B. holmesii O-specific polysaccharide has the following structure: {→2)-α-l-Rhap-(1→6)-α-d-Glcp-(1→4)-[β-d-GlcpNAc-(1→3]-α-d-Galp-(1→3)-α-d-GlcpNAc-(1→}n. The SDS-PAGE and serological cross-reactivities of the B. holmesii LPS suggested the similarity between the core oligosaccharides of B. holmesii ATCC 51541 and B. pertussis strain 606. The main oligosaccharide fraction contained a nonasaccharide. The comparative analysis of the NMR spectra of B. holmesii core oligosaccharide fraction with this of the B. pertussis strain 606 indicated that the investigated core oligosaccharides were identical.


2009 ◽  
Vol 344 (7) ◽  
pp. 894-900 ◽  
Author(s):  
Anna Maciejewska ◽  
Jolanta Lukasiewicz ◽  
Tomasz Niedziela ◽  
Zbigniew Szewczuk ◽  
Czeslaw Lugowski

Symmetry ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 94 ◽  
Author(s):  
Dario Fasino ◽  
Franca Rinaldi

The core–periphery structure is one of the key concepts in the structural analysis of complex networks. It consists of a partitioning of the node set of a given graph or network into two groups, called core and periphery, where the core nodes induce a well-connected subgraph and share connections with peripheral nodes, while the peripheral nodes are loosely connected to the core nodes and other peripheral nodes. We propose a polynomial-time algorithm to detect core–periphery structures in networks having a symmetric adjacency matrix. The core set is defined as the solution of a combinatorial optimization problem, which has a pleasant symmetry with respect to graph complementation. We provide a complete description of the optimal solutions to that problem and an exact and efficient algorithm to compute them. The proposed approach is extended to networks with loops and oriented edges. Numerical simulations are carried out on both synthetic and real-world networks to demonstrate the effectiveness and practicability of the proposed algorithm.


1997 ◽  
Vol 10 (7) ◽  
pp. 926-928 ◽  
Author(s):  
Mari-Anne Newman ◽  
Michael J. Daniels ◽  
J. Maxwell Dow

Pre-treatment of leaves of pepper (Capsicum annuum) with lipopolysaccharide (LPS) preparations from enteric bacteria and Xanthomonas campestris could prevent the hypersensitive response caused by an avirulent X. campestris strain. By use of a range of deep-rough mutants, the minimal structure in Salmonella LPS responsible for the elicitation of this effect was determined to be lipid A attached to a disaccharide of 2-keto-3-deoxyoctulosonate; lipid A alone and the free core oligosaccharide from a Salmonella Ra mutant were not effective. For Xanthomonas, the core oligosaccharide alone had activity although lipid A was not effective. The results suggest that pepper cells can recognize different structures within bacterial LPS to trigger alterations in plant response to avirulent pathogens.


2004 ◽  
Vol 271 (23-24) ◽  
pp. 4968-4977 ◽  
Author(s):  
Evelina L. Zdorovenko ◽  
Evgeny Vinogradov ◽  
Galina M. Zdorovenko ◽  
Buko Lindner ◽  
Olga V. Bystrova ◽  
...  

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