scholarly journals Supercritical Fluid Extract of Angelica sinensis and Zingiber officinale Roscoe Ameliorates TNBS-Induced Colitis in Rats

2019 ◽  
Vol 20 (15) ◽  
pp. 3816
Author(s):  
Jia Liu ◽  
Ling Yu ◽  
Nuolan Mo ◽  
Hai Lan ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Supercritical Fluid ◽  
Cell Model ◽  
Zingiber Officinale ◽  
Underlying Mechanism ◽  
Chinese Herbs ◽  
Angelica Sinensis ◽  
Bioactive Constituents ◽  
Trinitrobenzenesulfonic Acid ◽  
Zingiber Officinale Roscoe

Inflammatory bowel disease (IBD) is a worldwide healthcare problem calling for the development of new therapeutic drugs. Angelica sinensis and Zingiber officinale Roscoe are two common dietetic Chinese herbs, which are traditionally used for complementary treatment of gastrointestinal disorders. As bioactive constituents, volatile and pungent substances of these two herbs could be effectively extracted together by supercritical fluid extraction. In this study, the supercritical fluid extract of Angelica sinensis and Zingiber officinale Roscoe (AZ-SFE) was obtained by an optimized extraction process and it was chemically characterized. The anti-inflammatory effect and underlying mechanism of AZ-SFE were evaluated in a lipopolysaccharide (LPS)-induced RAW264.7 cell model and a 2, 4, 6-trinitrobenzenesulfonic acid (TNBS)-induced colitis rat model. AZ-SFE notably inhibited the production of NO in LPS-stimulated macrophages, and it inhibited the proliferation of Concanavalin A (Con A)-induced splenocytes with suppression of the Th1 immune response. In vivo, the study demonstrated that AZ-SFE significantly alleviated disease activity, colonic shortening, macroscopic damage and histological injury of TNBS-treated rats with reduction of oxidative stress, suppression of inflammatory cytokines, and modulation of hepcidin and serum iron. These findings suggested that AZ-SFE may be a promising supplement for current IBD therapy.

Vasculoprotective effects of ginger (Zingiber officinale Roscoe) and underlying molecular mechanisms

2021 ◽  
Author(s):  
Chao Li ◽  
Jie Li ◽  
Feng Jiang ◽  
Nikolay T. Tzvetkov ◽  
Jaroslaw O. Horbanczuk ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Molecular Mechanisms ◽  
Zingiber Officinale ◽  
Protective Effects ◽  
Bioactive Constituents ◽  
Zingiber Officinale Roscoe

Bioactive constituents of ginger are reviewed. Vascular protective effects of ginger and a variety of mechanisms are presented. Ginger and its components show vasculoprotective effects in clinical trials.

The Effect of Drying Methods on Physicochemical Properties of Nanostructured Zingiber officinale Rosc. (Ginger) Rhizome

2013 ◽  
Vol 667 ◽  
pp. 458-463 ◽  
Author(s):  
A. Norhidayah ◽  
A. Noriham ◽  
Mohamad Rusop
Keyword(s):  
Zingiber Officinale ◽  
Spherical Particles ◽  
Total Phenolic ◽  
Drying Methods ◽  
Particle Sizes ◽  
Drying Process ◽  
Bioactive Constituents ◽  
Freeze Dried ◽  
Zingiber Officinale Roscoe ◽  
Surface Morphologies

Zingiber officinale Roscoe, family of Zingiberaceae is classified as traditional medicinal that globally consumed as spices, food flavouring as well as remedies to treat women during confinement, to treat stomach upset and diarrhoea.The rhizome has been reported to contain abundant bioactive constituents which are mainly polyphenol and flavonoid. Therefore in this research the effect of cabinet drying (60°C) and freeze drying (-40°C) process during preparation of nanostructure Zingiber officinale Roscoe rhizome on particle sizes, surface morphology, FTIR, as well as Total Phenolic Content (TPC) and Total Flavonoid Content(TFC) were compared. Both drying process affect the particle sizes as well as TPC and TFC value. Finer particle size (254.3+ 9.33) and higher TPC (152.54 mg GAE/ g) and TFC (1.42 mgQE/g) were reported for cabinet dried nanostructure Zingiber officinale Rosc. rhizome as compared to freeze dried. The FESEM Photographs revealed that drying processes did affect the surface morphologies of nanostructure Zingiber officinale Rosc rhizome where cabinet dried produced solid spherical particles with a diameter around 100 – 200 nm and some smaller than100nm. Freeze dried consist of many nanoparticles having rod like structure. Both drying process did not significantly affect the presence of active compounds based on FTIR analysis.

Abstract 1362: Degradation of Cardiac Troponins in Atrial Fibrillation is Calpain-dependent

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Lei Ke ◽  
Anne-Jan Dijkhuis ◽  
Robert H Henning ◽  
Harm H Kampinga ◽  
Bianca J Brundel
Keyword(s):  
Atrial Fibrillation ◽  
Western Blotting ◽  
Troponin T ◽  
Cell Model ◽  
Underlying Mechanism ◽  
The Self ◽  
Calpain Inhibitor ◽  
Specific Degradation ◽  
Cardiac Troponins

Background: The self-perpeptuation of atrial fibrillation (AF) is caused by myocyte remodeling including degradation of cardiac troponins. Additionally, we observed enhanced calpain activity in human AF and in the tachypaced cell model for AF. We assessed the hypothesis that calpain plays a role in the degradation of cardiac troponins during AF. Methods: The tachypaced HL-1 atrial myocyte model was used to investigate degradation of endogenous cardiac troponin T, I and C (cTnT, cTnI, cTnC) as well as actin by Western-blotting. To study degradation of human cTnT, myocytes were transfected with V5-C-human cTnT. Inhibitors of calpain, caspase and proteasome were applied to study the underlying mechanism for degradation. In vivo cTn degradation was studied in atrial tissue from AF and sinus rhythm (SR) patients by Western-blotting. Results: Tachypacing (P, 3Hz) significantly and gradually induced the degradation of cTnT (Fig A ), cTnI and cTnC, compared to control (C, 1Hz), while actin were unaffected. The degradation was prevented by calpain inhibitor PD150606 (20μM), whereas caspase (CAS) and proteasome (MG) inhibition was not effective (Fig C ). Tachypacing of V5-C-human cTnT transfected HL-1 myocytes resulted in a specific degradation fragment of 25kDa, which was prevented by PD (Fig B ). In vivo, persistent AF was associated with specific degradation of cTnT, I and C, compared to patients with SR or paroxysmal AF. Actin levels were not changed. Conclusions: AF induces specific degradation of all cTn isoforms, which is mediated by calpain. Activation of calpain may represent a key component in linking cTn degradation, contractile dysfunction and the self-perpetuation of AF.

scholarly journals Benefits of Ginger and Its Constituent 6-Shogaol in Inhibiting Inflammatory Processes

2021 ◽  
Vol 14 (6) ◽  
pp. 571
Author(s):  
Iris Bischoff-Kont ◽  
Robert Fürst
Keyword(s):  
Zingiber Officinale ◽  
Mapk Signaling ◽  
Ppar Γ ◽  
Mediator Systems ◽  
Zingiber Officinale Roscoe ◽  
Ginger Rhizome ◽  
Inflammatory Processes ◽  
Anti Inflammatory

Ginger (Zingiber officinale Roscoe) is widely used as medicinal plant. According to the Committee on Herbal Medicinal Products (HMPC), dried powdered ginger rhizome can be applied for the prevention of nausea and vomiting in motion sickness (well-established use). Beyond this, a plethora of pre-clinical studies demonstrated anti-cancer, anti-oxidative, or anti-inflammatory actions. 6-Shogaol is formed from 6-gingerol by dehydration and represents one of the main bioactive principles in dried ginger rhizomes. 6-Shogaol is characterized by a Michael acceptor moiety being reactive with nucleophiles. This review intends to compile important findings on the actions of 6-shogaol as an anti-inflammatory compound: in vivo, 6-shogaol inhibited leukocyte infiltration into inflamed tissue accompanied with reduction of edema swelling. In vitro and in vivo, 6-shogaol reduced inflammatory mediator systems such as COX-2 or iNOS, affected NFκB and MAPK signaling, and increased levels of cytoprotective HO-1. Interestingly, certain in vitro studies provided deeper mechanistic insights demonstrating the involvement of PPAR-γ, JNK/Nrf2, p38/HO-1, and NFκB in the anti-inflammatory actions of the compound. Although these studies provide promising evidence that 6-shogaol can be classified as an anti-inflammatory substance, the exact mechanism of action remains to be elucidated. Moreover, conclusive clinical data for anti-inflammatory actions of 6-shogaol are largely lacking.

Inhibitory effects of ginger (Zingiber officinale Roscoe) essential oil on leukocyte migration in vivo and in vitro

2010 ◽  
Vol 65 (1) ◽  
pp. 241-246 ◽  
Author(s):  
Gessilda Alcantara Nogueira de Melo ◽  
Renata Grespan ◽  
Jefferson Pitelli Fonseca ◽  
Thiago Oliveira Farinha ◽  
Expedito Leite da Silva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Zingiber Officinale ◽  
Leukocyte Migration ◽  
Inhibitory Effects ◽  
Zingiber Officinale Roscoe

scholarly journals Antioxidant and Anti-Inflammatory Effects of Zingiber officinale roscoe and Allium subhirsutum: In Silico, Biochemical and Histological Study

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1383
Author(s):  
Nourhene Zammel ◽  
Mohd Saeed ◽  
Nouha Bouali ◽  
Salem Elkahoui ◽  
Jahoor M. Alam ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Silico ◽  
Histological Study ◽  
Hematological Parameters ◽  
Docking Simulation ◽  
Zingiber Officinale ◽  
Markers Of Inflammation ◽  
Zingiber Officinale Roscoe ◽  
Anti Inflammatory

In this study, the antioxidant and anti-inflammatory effects of Zingiber officinale roscoe and Allium subhirsutum aqueous extracts were examined in a carrageenan-induced acute inflammation model. Some markers of inflammation such as hematological parameters, fibrinogen and C-reactive protein were measured. Variables reflecting oxidative stress included thiobarbituric acid reactive substances (TBARS), advanced oxidation of protein products (AOPP), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione were determined in both inflamed foci and erythrocytes. The in silico molecular docking simulation showed that the main components of Zingiber officinale roscoe and Allium subhirsutum bound to toll-like receptor 6 (TLR6) with high affinities. Moreover, histological examinations of paw edema were carried out. Both Zingiber officinale roscoe and Allium subhirsutum ameliorated the induced inflammation and oxidative stress status as outlined by anti-edematous, antioxidant and anti-inflammatory activities. Our investigation lends pharmacological support to the medical uses of these spices in the management of inflammatory disorders and oxidative damage. The results of the in silico assay satisfactory explain the in vivo effects as compared with indomethacin.

scholarly journals ADGRG1 Is a Predictor of Chemoresistance and Poor Survival in Cervical Squamous Carcinoma

2021 ◽  
Vol 11 ◽  
Author(s):  
Shuo Zhang ◽  
Kui Guo ◽  
Ying Liang ◽  
Kun Wang ◽  
Shuyan Liu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Survival Data ◽  
Molecular Mechanisms ◽  
Cell Model ◽  
Squamous Carcinoma ◽  
Underlying Mechanism ◽  
Migration And Invasion ◽  
The Relationship

BackgroundCisplatin is the first-line chemotherapy for cervical cancer. Cisplatin resistance has always been one of the most significant barriers to acquiring better outcomes. However, the complex molecular mechanisms accounting for the phenomenon are not completely clear.MethodsConstruction of the cisplatin-resistant cell model of cervical cancer, then performing RNA sequencing and bioinformatic analysis of the differential expression genes. Then Adhesion G protein-coupled receptor G1 (ADGRG1) was screened out as our target gene. Gene Expression Profiling Interactive Analysis (GEPIA) was searched to show the expression level of ADGRG1 in cervical cancer and normal tissue. Kaplan-Meier Plotter (Kmplot) was used to explore the relationship of its expression with survival data. Tissue specimens were used to verify the relationship between the clinicopathological characteristics and ADGRG1 expression. Then we explored the roles of ADGRG1 in tumorigenesis through in vitro and in vivo assays.ResultsWe found the ADGRG1 was significantly overexpressed in cervical cancer tissues compared to corresponding normal tissues. Higher ADGRG1 expression was correlated with poor progress-free survival. Knockdown of ADGRG1 markedly suppressed cell proliferation, migration, and invasion and increased cell sensitivity to cisplatin in vitro. Similarly, the role of ADGRG1 knockdown on tumorigenicity and sensitivity to cisplatin treatment was verified in vivo. The underlying mechanism was explored by western blotting that ADGRG1 knockdown inhibited tumorigenesis by PI3K/Akt/mTOR signaling pathway.ConclusionADGRG1 acts as an oncogene to maintain tumorigenicity, migration, and invasion, and its depressed expression prompts sensitivity to cisplatin. Thus, ADGRG1 may represent a potential prognostic marker and possible therapeutic target for cervical cancer.

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