scholarly journals The Major Storage Protein in Potato Tuber Is Mobilized by a Mechanism Dependent on Its Phosphorylation Status

2019 ◽  
Vol 20 (8) ◽  
pp. 1889 ◽  
Author(s):  
Javier Bernal ◽  
Daniel Mouzo ◽  
María López-Pedrouso ◽  
Daniel Franco ◽  
Lucio García ◽  
...  
Keyword(s):  
Life Cycle ◽  
Storage Proteins ◽  
Solanum Tuberosum L ◽  
Vegetative Storage Proteins ◽  
Life Cycle Stages ◽  
Phosphorylation Mechanism ◽  
Major Storage Protein ◽  
Protein Dephosphorylation ◽  
Tuber Sprouting

The role of the protein phosphorylation mechanism in the mobilization of vegetative storage proteins (VSPs) is totally unknown. Patatin is the major VSP of the potato (Solanum tuberosum L.) tuber that encompasses multiple differentially phosphorylated isoforms. In this study, temporal changes in the phosphorylation status of patatin isoforms and their involvement in patatin mobilization are investigated using phosphoproteomic methods based on targeted two-dimensional electrophoresis (2-DE). High-resolution 2-DE profiles of patatin isoforms were obtained in four sequential tuber life cycle stages of Kennebec cultivar: endodormancy, bud break, sprouting and plant growth. In-gel multiplex identification of phosphorylated isoforms with Pro-Q Diamond phosphoprotein-specific stain revealed an increase in the number of phosphorylated isoforms after the tuber endodormancy stage. In addition, we found that the phosphorylation status of patatin isoforms significantly changed throughout the tuber life cycle (P < 0.05) using the chemical method of protein dephosphorylation with hydrogen fluoride-pyridine (HF-P) coupled to 2-DE. More specifically, patatin phosphorylation increased by 32% from endodormancy to the tuber sprouting stage and subsequently decreased together with patatin degradation. Patatin isoforms were not randomly mobilized because highly phosphorylated Kuras-isoforms were preferably degraded in comparison to less phosphorylated non-Kuras isoforms. These results lead us to conclude that patatin is mobilized by a mechanism dependent on the phosphorylation status of specific isoforms.

scholarly journals Parvatrema duboisi (Digenea: Gymnophallidae) Life Cycle Stages in Manila Clams, Ruditapes philippinarum, from Aphae-do (Island), Shinan-gun, Korea

2021 ◽  
Vol 59 (1) ◽  
pp. 83-88
Author(s):  
Bong-Kwang Jung ◽  
Taehee Chang ◽  
Hyejoo Shin ◽  
Seungwan Ryoo ◽  
Sooji Hong ◽  
...  
Keyword(s):  
Life Cycle ◽  
Post Infection ◽  
Ruditapes Philippinarum ◽  
Manila Clam ◽  
Long Tail ◽  
5.8S Rdna ◽  
Rdna Its2 ◽  
Life Cycle Stages ◽  
Second Intermediate Host

Life cycle stages, including daughter sporocysts, cercariae, and metacercariae, of Parvatrema duboisi (Dollfus, 1923) Bartoli, 1974 (Digenea: Gymnophallidae) have been found in the Manila clam Ruditapes philippinarum from Aphaedo (Island), Shinan-gun, Jeollanam-do, Korea. The daughter sporocysts were elongated sac-like and 307-570 (av. 395) μm long and 101-213 (av. 157) μm wide. Most of the daughter sporocysts contained 15-20 furcocercous cercariae each. The cercariae measured 112-146 (av. 134) μm in total length and 35-46 (av. 40) μm in width, with 69-92 (av. 85) μm long body and 39-54 (av. 49) μm long tail. The metacercariae were 210-250 (av. 231) μm in length and 170-195 (av. 185) μm in width, and characterized by having a large oral sucker, genital pore some distance anterior to the ventral sucker, no ventral pit, and 1 compact or slightly lobed vitellarium, strongly suggesting P. duboisi. The metacercariae were experimentally infected to ICR mice, and adults were recovered at day 7 post-infection. The adult flukes were morphologically similar to the metacercariae except in the presence of up to 20 eggs in the uterus. The daughter sporocysts and metacercariae were molecularly (ITS1-5.8S rDNA-ITS2) analyzed to confirm the species, and the results showed 99.8-99.9% identity with P. duboisi reported from Kyushu, Japan and Gochang, Korea. These results confirmed the presence of various life cycle stages of P. duboisi in the Manila clam, R. philippinarum, playing the role of the first as well as the second intermediate host, on Aphae-do (Island), Shinan-gun, Korea.

The Influence of Socializing Agents on Female Collegiate Volleyball Players

1982 ◽  
Vol 4 (3) ◽  
pp. 267-279 ◽  
Author(s):  
Maureen R. Weiss ◽  
Annelies Knoppers
Keyword(s):  
Life Cycle ◽  
Significant Others ◽  
Physical Education Teachers ◽  
Big Ten ◽  
Life Cycle Stages ◽  
Volleyball Players ◽  
Methodological Procedures ◽  
Socializing Agents ◽  
Active Sport

The purpose of this study was twofold: (a) to replicate and extend the earlier findings of Greendorfer (1977) by examining the role of significant others on the socialization of female volleyball players, and (b) to clarify the use of various statistical methodologies in sport socialization research. Players (N = 95) competing in the 1979 Big Ten volleyball championships responded to a sport socialization questionnaire designed to assess the degree of influence of socializing agents on active sport involvement. Descriptive statistics revealed that female volleyball players were surrounded by significant others who strongly supported and encouraged their participation throughout their life cycle. Multiple regression analyses revealed that, of the significant others who supported participation, parents, peers, and physical education teachers/coaches collectively had a significant influence on sport involvement only during the participant's childhood. Brothers were significant agents of sport socialization during the player's childhood and college years; no other agents reached significance for any of the life cycle stages. The discrepant results between the two analyses of this study, and previous socialization studies, were attributed to homogeneity of the sample and the statistical methods used. Recommendations are made about the use of statistical and methodological procedures in future socialization research.

scholarly journals The Relationship between Corporate Social Responsibility Reporting and Firm Performance and the Moderating Role of Firm Life Cycle Stages: Evidence from China

2021 ◽  
Vol 13 (18) ◽  
pp. 10038
Author(s):  
Naveed Jan ◽  
Arodh Lal Karn ◽  
Zeyun Li ◽  
Xiyu Liu
Keyword(s):  
Corporate Social Responsibility ◽  
Life Cycle ◽  
Firm Performance ◽  
Social Responsibility ◽  
Csr Reporting ◽  
Life Cycle Stages ◽  
Corporate Social Responsibility Reporting ◽  
Corporate Social ◽  
Moderating Role

This study aims to investigate the relationship of firm performance and corporate social responsibility reporting and the moderating role of a firm’s life cycle stages in Chinese listed companies. We used the sample of all A-share listed firms on the Shanghai and Shenzhen stock exchanges for the period 2010 to 2020. The authors used pooled ordinary least squares (OLS) regression as a baseline methodology. Our regression results show that positive Corporate social responsibility (CSR) activity significantly reduces the performance of the firm. In addition, the negative link between positive Corporate social responsibility and a firm’s performance is more pronounced for firms in mature life cycle stages. Our results are robust to alternative proxy measures of ROA for firm performance, corporate social responsibility reporting, and life cycle stages. To control the possible problem of endogeneity, we use a one-year lag and 2SLS least squares regression. We find that firm performance has a statistically significant influence on CSR reporting. Moreover, we see that firms with high performance are more likely to report CSR activities than low-performance firms. Additionally, six out of ten control variables (Independent Director, Board Shares, State Owned Enterprise, Board Meeting, Chief executive officer Duality, and Firm Growth) have positive influences on CSR reporting. These findings hold for a set of robustness tests. Our results have implications for the development of CSR reporting in developing countries such as China. Our research suggests that, in China, firms with better financial performance undertake more CSR reporting. This paper contributes to the existing literature by investigating the effect of firm performance on CSR reporting and the moderating role of a firm’s life cycle stages in Chinese listed companies. Additionally, this paper enriches the current literature on CSR reporting and highlights the importance of a firm’s financial performance for better environmental performance and reporting.

scholarly journals Does Firm Life Cycle Impact Corporate Investment Efficiency?

2020 ◽  
Vol 13 (1) ◽  
pp. 197
Author(s):  
Bilal Ahmed ◽  
Minhas Akbar ◽  
Tanazza Sabahat ◽  
Saqib Ali ◽  
Ammar Hussain ◽  
...  
Keyword(s):  
Life Cycle ◽  
Fixed Effects ◽  
Corporate Investment ◽  
Ordinary Least Squares ◽  
Investment Efficiency ◽  
Investment Policies ◽  
Firm Life Cycle ◽  
Life Cycle Stages ◽  
Growth Firms

Corporate investment efficiency (CIE) is an imperative factor influencing the smooth functioning and financial sustainability of an enterprise. The role of a firm life cycle on risk and performance fundamentals has been extensively explored in the literature. However, it remains unclear as to whether the life cycle stages of a firm have any impact on corporate investment efficiency. This paper investigates the role of firm life cycle stages (FLCS) in determining the investment efficiency of 351 Pakistani non-financial listed firms over the course of 12 years (2005–2016). It used panel data fixed effects and ordinary least squares (OLS) techniques to empirically examine the proposed relationship. By employing Dickinson’s FLCS measure, we found that CIE was lower during the introduction and decline stages and higher at the growth and maturity stages of a firm’s life cycle. Moreover, the results of regression analysis revealed that mature firms enjoyed the highest level of investment efficiency followed by the growth firms. Overall, CIE exhibited an inverted U-shaped trend across FLCS. In addition, the findings corroborated the idea that the sample firms could not sustain their investment efficiency when they moved along different stages of the life cycle. Thus, policymakers are suggested to customize their investment policies for each stage of FLC to attain sustainable financial performance throughout the life of a firm.

Green Diabetes Summit 2021

2021 ◽  
pp. 193229682110498
Author(s):  
Kevin T. Nguyen ◽  
Nicole Y. Xu ◽  
Jennifer Y. Zhang ◽  
Trisha Shang ◽  
Ashley Y. DuBord ◽  
...  
Keyword(s):  
United States ◽  
Life Cycle ◽  
Waste Management ◽  
Home Care ◽  
The United States ◽  
Stakeholder Groups ◽  
Life Cycle Stages ◽  
Diabetes Technology ◽  
Proper Disposal

On July 21, 2021, Diabetes Technology Society convened the virtual Green Diabetes Summit. The event consisted of 23 representatives from key stakeholder groups based in both the United States and Europe. The purposes of the summit were to (1) provide background on the complexity of addressing sustainability-related issues, including waste management, of diabetes devices from many different perspectives along the products’ life cycle stages, and (2) determine the feasibility and role of a coalition of stakeholders to find solutions, particularly in the design, use, and proper disposal of diabetes devices used in home care that no one stakeholder can resolve on their own.

ASPECTS IN BUILDING A COMPANY WEB-SITE AND ITS ROLE FOR THE DEVELOPMENT OF BUSINESS

2018 ◽  
pp. 117-124
Author(s):  
Petar Halachev ◽  
Victoria Radeva ◽  
Albena Nikiforova ◽  
Miglena Veneva
Keyword(s):  
Life Cycle ◽  
Web Site ◽  
The Internet ◽  
A Company ◽  
The Web ◽  
Design Construction ◽  
Made In ◽  
Site Types

This report is dedicated to the role of the web site as an important tool for presenting business on the Internet. Classification of site types has been made in terms of their application in the business and the types of structures in their construction. The Models of the Life Cycle for designing business websites are analyzed and are outlined their strengths and weaknesses. The stages in the design, construction, commissioning, and maintenance of a business website are distinguished and the activities and requirements of each stage are specified.

FINANCING THE DEVELOPMENT OF MUNICIPAL ENTITIES AT DIFFERENT LIFE CYCLE STAGES: PUBLIC AND PRIVATE RESOURCES

2019 ◽  
Vol 0 (3) ◽  
pp. 53-60 ◽  
Author(s):  
T.Yu. Altufyeva ◽  
◽  
P.A. Ivanov ◽  
G.R. Sakhapova ◽  
◽  
...  
Keyword(s):  
Life Cycle ◽  
Public And Private ◽  
Life Cycle Stages
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