scholarly journals Comparative Proteomic Profiling between Each of Two Consecutive Developmental Stages of the Solanum Fruit Fly, Bactrocera latifrons (Hendel)

2018 ◽  
Vol 19 (7) ◽  
pp. 1996 ◽  
Author(s):  
Chiou Chang ◽  
Scott Geib
Keyword(s):  
Developmental Stages ◽  
Fruit Fly ◽  
Proteomic Profiling ◽  
Bactrocera Latifrons

scholarly journals APLIKASI BEBERAPA EKSTRAK TANAMAN SEBAGAI BAHAN PERANGKAP LALAT BUAH (Bactrocera sp.)

AGRICA ◽  
2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Sri Wahyuni ◽  
Petrus Deornay
Keyword(s):  
Plant Extract ◽  
Diversity Index ◽  
Fruit Fly ◽  
Fruit Flies ◽  
Seed Extract ◽  
Average Value ◽  
Bactrocera Latifrons

Application Some Extracts of plant as Trap Material of Fruit Flies (Bactrocera sp). This study aims to determine: 1) the ability of Pala, Basil and Clove seed extract as a trap material for Bactrocera sp. 2) the best plant extract as a trap material for Bactrocera sp. The extraction activity was carried out at the Laboratory of the Faculty of Agriculture, University of Flores, while the fruit fly trap installation was carried out in Ndengga Rongge Village (± 913 m asl) and Lokoboko (± 698 m asl) in Ende Regency. The study was conducted for 3 months, namely in April - June 2018. Observation variables used included the types of fruit fly found in the field and calculated the level of diversity, abundance and dominance of pests and the capture power of each trap in the type of extraction. There are three types of fruit flies trapped in 3 types of attractants in tomato plantations, namely Bactrocera papaya, Bactrocera umbrosa Fabricius and Bactrocera latifrons Handel.  Diversity index of Bactrocera sp. in the research location is still relatively low.  The highest abundance is B.papayae with the average value of H '= 1.86 and the lowest is  B.latifros (H’= 0,07). There is no dominance of type at research location which indicates that the condition of the ecosystem is still stable. Nutmeg extract is the best attractant trap material as an attractant material in field flies

scholarly journals Population dynamics of pre-imaginal stages of olive fruit fly Bactrocera oleae Gmel. (Diptera, Tephritidae) in the region of Bar (Montenegro)

2013 ◽  
Vol 28 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Tatjana Perovic ◽  
Snjezana Hrncic
Keyword(s):  
Population Dynamics ◽  
Developmental Stages ◽  
Fruit Fly ◽  
Bactrocera Oleae ◽  
Yield Reduction ◽  
Olive Fruit Fly ◽  
Application Timing ◽  
Olive Fruit ◽  
Fruit Drop

Olive fruit fly is the most harmful pest of olive fruits and important for oil production. Damage involves yield reduction as a consequence of premature fruit drop, but also a reduced quality of olive oil and olive products. There is little available data regarding the biology of Bactrocera oleae in Montenegro. Knowledge of the pest life cycle and development would improve optimization of insecticide application timing and protection of fruits, and reduce adverse effects on the environment. Investigation was conducted on the Zutica variety in an olive grove located in Bar during a three-year period. Population dynamics of the pre-imaginal stages and level of fruit infestation were monitored from mid-July until the end of October. The results of this three-year investigation showed that the beginning of infestation was always at the end of July. It was also found that, depending on environmental conditions, the level of infestation was low until the end of August. In September and October it multiplied, and reached maximum by the end of October. Regarding infestation structure, eggs and first instar larvae were the dominant developmental stages of the pest until the middle of September. From mid-September until mid-October all developmental stages (eggs, larvae, pupae) were equally present in infested fruits. Pupae, cocoons and abandoned galleries prevailed until the harvest.

scholarly journals Identification and Expression Analysis of Snf2 Family Proteins in Tomato (Solanum lycopersicum)

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Dongdong Zhang ◽  
Sujuan Gao ◽  
Ping Yang ◽  
Jie Yang ◽  
Songguang Yang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Solanum Lycopersicum ◽  
Developmental Stages ◽  
Profile Analysis ◽  
Nucleosome Position ◽  
Fruit Fly ◽  
Amino Acid Residues ◽  
Expression Profile Analysis ◽  
Wide Range ◽  
Chromatin Remodeling Complexes

As part of chromatin-remodeling complexes (CRCs), sucrose nonfermenting 2 (Snf2) family proteins alter chromatin structure and nucleosome position by utilizing the energy of ATP, which allows other regulatory proteins to access DNA. Plant genomes encode a large number of Snf2 proteins, and some of them have been shown to be the key regulators at different developmental stages in Arabidopsis. Yet, little is known about the functions of Snf2 proteins in tomato (Solanum lycopersicum). In this study, 45 Snf2s were identified by the homologous search using representative sequences from yeast (S. cerevisiae), fruit fly (D. melanogaster), and Arabidopsis (A. thaliana) against the tomato genome annotation dataset. Tomato Snf2 proteins (also named SlCHRs) could be clustered into 6 groups and distributed on 11 chromosomes. All SlCHRs contained a helicase-C domain with about 80 amino acid residues and a SNF2-N domain with more variable amino acid residues. In addition, other conserved motifs were also identified in SlCHRs by using the MEME program. Expression profile analysis indicated that tomato Snf2 family genes displayed a wide range of expressions in different tissues and some of them were regulated by the environmental stimuli such as salicylic acid, abscisic acid, salt, and cold. Taken together, these results provide insights into the functions of SlCHRs in tomato.

scholarly journals Versatile proteome labelling in fruit flies with SILAF

2019 ◽  
Author(s):  
Florian A. Schober ◽  
Ilian Atanassov ◽  
David Moore ◽  
Anna Wedell ◽  
Christoph Freyer ◽  
...  
Keyword(s):  
Protein Turnover ◽  
Cell Biology ◽  
Metabolic Regulation ◽  
Developmental Stages ◽  
Fruit Fly ◽  
Phosphorylation Sites ◽  
Turnover Rates ◽  
Post Translational Modification ◽  
Stable Isotope Labelling ◽  
Rapid Generation

ABSTRACTDrosophila melanogaster has been a working horse of genetics and cell biology for more than a century. However, proteomic-based methods have been limited due to technical obstacles, especially the lack of reliable labelling methods. Here, we advanced a chemically defined food source into stable-isotope labelling of amino acids in flies (SILAF). It allows for the rapid generation of a large number of flies with full incorporation of lysine-6. SILAF followed by fractionation and enrichment gave proteomic insights at a depth of 5,966 proteins and 7,496 phosphorylation sites, which substantiated metabolic regulation on enzymatic level. Furthermore, the label can be traced and predicts protein turnover rates during different developmental stages. The ease and versatility of the method actuates the fruit fly as an appealing model in proteomic and post-translational modification studies and it enlarges potential metabolic applications based on heavy amino acid diets.

Fly-on-a-Chip: Microfluidics for Drosophila melanogaster Studies

2019 ◽  
Vol 11 (12) ◽  
pp. 425-443 ◽  
Author(s):  
Alireza Zabihihesari ◽  
Arthur J Hilliker ◽  
Pouya Rezai
Keyword(s):  
Drosophila Melanogaster ◽  
Developmental Stages ◽  
Model Organism ◽  
Fruit Fly ◽  
In Vitro Assays ◽  
Behavioral Studies ◽  
And Behavior ◽  
Manual Manipulation

Abstract The fruit fly or Drosophila melanogaster has been used as a promising model organism in genetics, developmental and behavioral studies as well as in the fields of neuroscience, pharmacology, and toxicology. Not only all the developmental stages of Drosophila, including embryonic, larval, and adulthood stages, have been used in experimental in vivo biology, but also the organs, tissues, and cells extracted from this model have found applications in in vitro assays. However, the manual manipulation, cellular investigation and behavioral phenotyping techniques utilized in conventional Drosophila-based in vivo and in vitro assays are mostly time-consuming, labor-intensive, and low in throughput. Moreover, stimulation of the organism with external biological, chemical, or physical signals requires precision in signal delivery, while quantification of neural and behavioral phenotypes necessitates optical and physical accessibility to Drosophila. Recently, microfluidic and lab-on-a-chip devices have emerged as powerful tools to overcome these challenges. This review paper demonstrates the role of microfluidic technology in Drosophila studies with a focus on both in vivo and in vitro investigations. The reviewed microfluidic devices are categorized based on their applications to various stages of Drosophila development. We have emphasized technologies that were utilized for tissue- and behavior-based investigations. Furthermore, the challenges and future directions in Drosophila-on-a-chip research, and its integration with other advanced technologies, will be discussed.

74 PROTEOMIC PROFILING DURING FETAL DEVELOPMENT OF BOVINE CLONES

2010 ◽  
Vol 22 (1) ◽  
pp. 195
Author(s):  
B. Picard ◽  
B. Meunier ◽  
Y. Heyman ◽  
P. Chavatte-Palmer ◽  
I. Cassar-Malek
Keyword(s):  
Statistical Analysis ◽  
Energy Metabolism ◽  
First Generation ◽  
Muscle Development ◽  
Technical Assistance ◽  
Developmental Stages ◽  
First Year ◽  
Proteomic Profiling ◽  
Semitendinosus Muscle ◽  
Regulation Of Cell Cycle

Previous data have shown that bovine clones display a delay in their muscle differentiation during their first year postnatal (Jurie et al. 2009 Animal 2, 244-250). This delay could originate from perturbations in fetal muscle development as illustrated by lower numbers and degree of organization of the first generation of myotubes at 60 dpc and by their lower energy metabolism and their myosin heavy chain pattern at 260 dpc (Cassar-Malek et al. 2009 Proc. XIth ISRP abst). In order to understand the mechanisms underlying the delay in myogenesis, we have performed a comparative proteomic analysis of the semitendinosus muscle in fetuses derived from somatic nuclear transfer and their control counterparts obtained after AI at these two important developmental stages. Two-dimensional electrophoresis using a 3-10 non-linear pH gradient were performed on samples at 60 dpc (in a group of Holstein animals and a group of Charolais animals, n = 4 fetuses per lot) and at 260 dpc (in a group of Holstein animals, n = 4 fetuses per lot). Gel analysis was conducted using the image analysis SameSpots (Nonlinear Dynamics, Newcastle upon Tyne, UK). As expected, the protein profiles were visually very different between developmental stages. At 60 dpc, 463 spots common to all gels were retained for statistical analysis using the significance analysis of microarrays (SAM) method (FDR <5%; Meunier et al. 2005 Anal. Biochem. 340, 226-230). At 260 dpc, 491 spots were selected for SAM analysis. The statistical analysis revealed a small number of differential spots (9 and 10 spots, respectively, at 60 dpc in Holstein and Charolais, and 10 spots at 260 dpc Holstein). The differential spots were excised from the gels and their identification by mass spectrometry is in progress. Preliminary results are presented in Table 1. In conclusion, subtle changes in the muscle proteome were detected in clones v. controls. Some of them were related to the regulation of cell cycle/apoptosis at 60 dpc and to energy metabolism and chaperone activity at 260 dpc. The relevance of these changes will be further explored using bioinformatics tools. Table 1.Examples of identified spots with differential abundance between clones and controls The authors thank C. Barboiron for excellent technical assistance.

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