scholarly journals Isolation and Characterization of Simple Sequence Repeats (SSR) Markers from the Moss Genus Orthotrichum Using a Small Throughput Pyrosequencing Machine

2012 ◽  
Vol 13 (6) ◽  
pp. 7586-7593 ◽  
Author(s):  
Jakub Sawicki ◽  
Mirosław Kwaśniewski ◽  
Monika Szczecińska ◽  
Karolina Chwiałkowska ◽  
Monika Milewicz ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Isolation And Characterization ◽  
Simple Sequence

scholarly journals Characterization of vine varieties by SSR markers

2013 ◽  
Vol 6 (2) ◽  
pp. 227-234 ◽  
Author(s):  
Ivana Dokupilová ◽  
Ernest Šturdík ◽  
Daniel Mihálik
Keyword(s):  
European Union ◽  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Pinot Noir ◽  
The European Union ◽  
Grape Genome ◽  
Grape Varieties ◽  
Simple Sequence ◽  
Varietal Characterization

Abstract Nowadays, there are well known PCR-based techniques to detect polymorphism in plants. Marker technologies which are applied in breeding and varietal characterization can be exchanged across laboratories with standardization to yield reproducible results. This article describes applying of simple sequence repeats (SSR) markers in European laboratories, in which the reproducibility of these popular markers are examined. One of the European Union interests is characterization of grape varieties and preparation of International Vitis catalogue for comparison global varieties as Chardonnay, Sauvignon, Pinot noir with the domestic for each other country. Grape genome contains of many SSR, which are highly polymorphic. This review provides the latest information in SSR research including novels in research, comparison, development and application of microsatellites.

Genetic Diversity and Characterization of a Core Collection of Malus Germplasm Using Simple Sequence Repeats (SSRs)

2011 ◽  
Vol 30 (4) ◽  
pp. 827-837 ◽  
Author(s):  
Sarah M. Potts ◽  
Yuepeng Han ◽  
M. Awais Khan ◽  
Mosbah M. Kushad ◽  
A. Lane Rayburn ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeats ◽  
Core Collection ◽  
Simple Sequence

scholarly journals Development of EST-derived SSR Markers with Long-core Repeat in Olive and Their Use for Paternity Testing

2013 ◽  
Vol 138 (4) ◽  
pp. 290-296 ◽  
Author(s):  
Raúl De la Rosa ◽  
Angjelina Belaj ◽  
Antonio Muñoz-Mérida ◽  
Oswaldo Trelles ◽  
Inmaculada Ortíz-Martín ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Expressed Sequence Tag ◽  
Paternity Testing ◽  
High Level ◽  
Repeated Motifs ◽  
Expressed Sequence ◽  
Simple Sequence ◽  
Olive Breeding ◽  
Genomic Project

In the present work, a set of eight new hexa-nucleotide simple sequence repeats (SSRs) is reported in olive (Olea europaea L). These SSRs loci were generated on the basis of expressed sequence tag (EST) sequences in the frame of an olive genomic project. The markers showed a high level of polymorphism when tested on a set of cultivars used as genitors in the olive breeding program of Córdoba, Spain. The long-core repeat motif of these markers allows a wider separation among alleles, thus permitting an accurate genotyping. Besides, these markers showed comparable levels of polymorphism to di-nucleotide SSRs, the only ones so far reported in olive. Selected on the basis of their discrimination capacity, four of the eight SSRs were used to test their ability for paternity testing in a total of 81 seedlings coming from 12 crosses. The paternity testing showed that seven crosses matched the alleged paternity and the remaining five were products of illicit pollinations. These results exactly matched with previous paternity testing performed with di-nucleotide SSR markers. These results demonstrate the usefulness of the developed hexa-nucleotide repeated motifs for checking the paternity of breeding progenies and suggest their use on variability studies.

scholarly journals Isolation and characterization of EST-SSR markers for Astilboides tabularis (Saxifragaceae), endangered species in Korea

2018 ◽  
Vol 48 (3) ◽  
pp. 195-200
Author(s):  
Eui-Kwon JUNG ◽  
Dae-Hyun KANG ◽  
Ki-Oug YOO ◽  
Myounghai KWAK ◽  
Young-Dong KIM ◽  
...  
Keyword(s):  
Endangered Species ◽  
Ssr Markers ◽  
Isolation And Characterization
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