Molecular characterization of early blight disease resistant and susceptible potato cultivars using random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers

2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Mahmoud H. El_Komy
Keyword(s):  
Molecular Characterization ◽  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Early Blight ◽  
Random Amplified Polymorphic Dna ◽  
Potato Cultivars ◽  
Disease Resistant ◽  
Blight Disease ◽  
Simple Sequence

Molecular characterization of Lentil (Lens culinaris Medikus) genotypes through Simple sequence repeat (SSR) markers

2018 ◽  
Vol 8 (1) ◽  
pp. 65
Author(s):  
Ashish Kumar Pandey ◽  
Rakesh Singh Sengar ◽  
Ashok Kumar ◽  
Pooran Chand ◽  
Raghbendra Yadav ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Lens Culinaris ◽  
Sequence Repeat ◽  
Simple Sequence

scholarly journals Molecular characterization of potato cultivars using SSR markers

2011 ◽  
Vol 29 (4) ◽  
pp. 542-547 ◽  
Author(s):  
Patrícia Favoretto ◽  
Elizabeth Ann Veasey ◽  
Paulo César Tavares de Melo
Keyword(s):  
Molecular Characterization ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Binary Data ◽  
Potato Crop ◽  
Similarity Coefficient ◽  
High Similarity ◽  
Commercial Cultivars ◽  
Simple Sequence

The potato crop has a very narrow genetic base, so the use of molecular markers is a very important tool in the characterization of germplasm banks and evaluation of genetic divergence. The objective of this study was to identify, using microsatellite or simple sequence repeat (SSR) markers, 38 accessions of potato from two collections of commercial cultivars. For the molecular characterization 10 loci were used, generating a total of 46 alleles, which were analyzed as binary data. A cluster analysis was performed with the Jaccard´s similarity coefficient and the UPGMA method, using the software NTSYSpc. On average, the number of alleles per locus was 4.6, ranging from two alleles for primers STM1049, STM 1053 and STM 1104 to 12 alleles per locus for primer STM0019a. Of the 46 alleles, only five were monomorphic, therefore presenting 89.1% polymorphism. The polymorphism information content (PIC) varied from 0.13 to 0.86, with an average of 0.54. The Jaccard´s coefficient varied from 0.41 to 0.93, showing high genetic variability among accessions. Two possible duplicates [Atlantic (Canada) and Atlantic (Chile), and Colorado and Ágata (EPAMIG) (duplicates with these SSRs, which did not separate them)] were identified. High similarity was also shown by cultivars Chipie and Melodie (EPAMIG), Voyager and Gourmandine (EPAMIG), Eole and Caesar (EPAMIG), and Cupido and Santé (Pirassu). The most genetically divergent accessions (Lady Rosetta and HPC-7B) were also identified.

scholarly journals Molecular Characterization of Maize (Zea mays L.) Inbred Lines using Simple Sequence Repeats (SSR) Marker

2020 ◽  
Vol 9 (6) ◽  
pp. 3158-3164
Author(s):  
Urusha Zareen ◽  
Birender Singh ◽  
Bishun Deo Prasad ◽  
S. S. Mandal ◽  
R. B. P. Nirala ◽  
...  
Keyword(s):  
Zea Mays ◽  
Molecular Characterization ◽  
Simple Sequence Repeats ◽  
Ssr Marker ◽  
Zea Mays L ◽  
Inbred Lines ◽  
Simple Sequence
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