scholarly journals Expression and Clinical Role of Protein of Regenerating Liver (PRL) Phosphatases in Ovarian Carcinoma

2011 ◽  
Vol 12 (2) ◽  
pp. 1133-1145 ◽  
Author(s):  
Reuven Reich ◽  
Shany Hadar ◽  
Ben Davidson
Keyword(s):  
Ovarian Carcinoma ◽  
Regenerating Liver ◽  
Clinical Role ◽  
Prl Phosphatases

scholarly journals Expression and clinical role of the dipeptidyl peptidases DPP8 and DPP9 in ovarian carcinoma

2018 ◽  
Vol 474 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Marta Brunetti ◽  
Arild Holth ◽  
Ioannis Panagopoulos ◽  
Anne Cathrine Staff ◽  
Francesca Micci ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
Clinical Role ◽  
Dipeptidyl Peptidases

scholarly journals The Biological and Clinical Role of the Long Non-Coding RNA LOC642852 in Ovarian Carcinoma

2020 ◽  
Vol 21 (15) ◽  
pp. 5237 ◽  
Author(s):  
Natalie Filippov-Levy ◽  
Reuven Reich ◽  
Ben Davidson
Keyword(s):  
Matrix Metalloproteinase ◽  
Cell Signaling ◽  
Ovarian Carcinoma ◽  
Serous Carcinoma ◽  
Spheroid Formation ◽  
Clinical Role ◽  
Cerna Network ◽  
Non Coding Rna ◽  
Long Non Coding Rna

The objective of the present study was to analyze the biological and clinical role of the long non-coding RNA LOC642852 in ovarian carcinoma (OC). LOC642852 expression was analyzed in seven OC cell lines (OVCAR-3, OVCAR-8, OVCA 433, OVCA 429, OC 238, DOV13, ES-2) and 139 high-grade serous carcinoma (HGSC) specimens (85 effusions, 54 surgical specimens). Following LOC642852 knockout (KO) using the CRISPR/Cas9 system, OVCAR-8 HGSC cells were analyzed for spheroid formation, migration, invasion, proliferation, matrix metalloproteinase (MMP) activity, and expression of cell signaling proteins. OVCAR-8 cells with LOC642852 KO were significantly less motile and less invasive compared to controls, with no differences in spheroid formation, proliferation, or matrix metalloproteinase (MMP) activity. Total Akt and Erk levels were comparable in controls and KO cells, but their phosphorylation was significantly increased in the latter. In clinical specimens, LOC642852 was overexpressed in ovarian tumors and omental/peritoneal metastases compared to effusion specimens (p = 0.013). A non-significant trend for shorter overall (p = 0.109) and progression-free (p = 0.056) survival was observed in patients with HGSC effusions with high LOC642852 levels. Bioinformatics analysis showed potential roles for LOC642852 as part of the TLE3/miR-221-3p ceRNA network and in relation to the FGFR3 protein. In conclusion, LOC642852 inactivation via CRISPR/Cas9 affects cell signaling, motility, and invasion in HGSC cells. LOC642852 is differentially expressed in HGSC cells at different anatomical sites. Its potential role in regulating the TLE3/miR-221-3p ceRNA network and FGFR3 merits further research.

Different Expression and Clinical Role of S100A4 in Serous Ovarian Carcinoma at Different Anatomic Sites

Tumor Biology ◽  
2009 ◽  
Vol 30 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Gunhild M. Mælandsmo ◽  
Vivi Ann Flørenes ◽  
Mai T.P. Nguyen ◽  
Kjersti Flatmark ◽  
Ben Davidson
Keyword(s):  
Ovarian Carcinoma ◽  
Clinical Role ◽  
Serous Ovarian Carcinoma

Expression and clinical role of the bric-a-brac tramtrack broad complex/poxvirus and zinc protein NAC-1 in ovarian carcinoma effusions

2007 ◽  
Vol 38 (7) ◽  
pp. 1030-1036 ◽  
Author(s):  
Ben Davidson ◽  
Aasmund Berner ◽  
Claes G. Trope' ◽  
Tian-Li Wang ◽  
Ie-Ming Shih
Keyword(s):  
Ovarian Carcinoma ◽  
Clinical Role ◽  
Broad Complex ◽  
Zinc Protein

Expression and clinical role of DJ-1, a negative regulator of PTEN, in ovarian carcinoma

2008 ◽  
Vol 39 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Ben Davidson ◽  
Rivka Hadar ◽  
Anat Schlossberg ◽  
Tamar Sternlicht ◽  
Ana Slipicevic ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
Negative Regulator ◽  
Clinical Role

scholarly journals Role of the Exosome Secretion Machinery in Ovarian Carcinoma: In Vitro and In Vivo Models

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Esther Channah Broner ◽  
Hadil Onallah ◽  
Tali Tavor Re’em ◽  
Ben Davidson ◽  
Reuven Reich
Keyword(s):  
Ovarian Carcinoma ◽  
Mrna Expression ◽  
Carcinoma Cells ◽  
Serous Carcinoma ◽  
Clinical Role ◽  
In Vivo Models ◽  
Ovarian Carcinoma Cells

Objective. We recently reported on the expression and clinical role of molecules that mediate exosome secretion in high-grade serous carcinoma. In the present study, the biological role of these molecules was analyzed. Methods. OVCAR8 and ES-2 ovarian carcinoma cells were studied using a combination of CRISPR/Cas9 technology and two 3D in vitro models—spheroids emulating effusions and alginate scaffolds representing solid lesions. Isolation of exosomes was validated by electron microscopy. TSAP6, NSMASE2, RAB27A, and RAB27B mRNA and protein levels were analyzed using qRT-PCR and Western blotting, respectively. Tumor aggressiveness was studied in vitro using scratch assay, invasion assay, and matrix metalloproteinase (MMP) activity assay and in vivo using a mouse model. Results. In OVCAR8 cells, mRNA expression of TSAP6 and RAB27A was significantly higher in spheroids compared to scaffolds, whereas the opposite was true for NSMASE2 mRNA. In ES-2 cells, TSAP6 and RAB27B mRNA expression was significantly higher in spheroids versus scaffolds. In addition, nSMase2 and TSAP6 protein expression was significantly higher in scaffolds compared to spheroids. CRISPR-edited cells with silencing of NSMASE2, TSAP6, and RAB27A/B had reduced migration, invasion, and MMP activity. Additionally, knockout (KO) of these molecules resulted in significantly diminished exosome secretion. In vivo assay showed that when injected to mice, OVCAR8 RAB27A/B KO cells, as opposed to control OVCAR8 cells, did not form ascites or visible tumor lesions and had reduced MMP expression. Conclusion. The present study provides evidence that different models for culturing ovarian carcinoma cells affect the expression of molecules mediating exosome secretion and that these molecules have a tumor-promoting role. Silencing these molecules may consequently have therapeutic relevance in this cancer.

The clinical role of phospholipase A2 isoforms in advanced-stage ovarian carcinoma

2006 ◽  
Vol 103 (3) ◽  
pp. 831-840 ◽  
Author(s):  
Michal Gorovetz ◽  
Mark Baekelandt ◽  
Aasmund Berner ◽  
Claes G. Trope' ◽  
Ben Davidson ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Ovarian Carcinoma ◽  
Advanced Stage ◽  
Clinical Role

Comments on expanding the clinical role of psychologists.

1996 ◽  
Vol 51 (3) ◽  
pp. 216-218 ◽  
Author(s):  
Rachel G. Klein
Keyword(s):  
Clinical Role
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