scholarly journals Transcriptome Analysis of Two Rice Varieties Contrasting for Nitrogen Use Efficiency under Chronic N Starvation Reveals Differences in Chloroplast and Starch Metabolism-Related Genes

Genes ◽  
2018 ◽  
Vol 9 (4) ◽  
pp. 206 ◽  
Author(s):  
Subodh Sinha ◽  
Amitha Sevanthi V. ◽  
Saurabh Chaudhary ◽  
Punit Tyagi ◽  
Sureshkumar Venkadesan ◽  
...  
Keyword(s):  
Nitrogen Use Efficiency ◽  
Transcriptome Analysis ◽  
Starch Metabolism ◽  
Rice Varieties ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
N Starvation

Comparative genome and transcriptome analysis unravels key factors of nitrogen use efficiency in Brassica napus L

2019 ◽  
Vol 43 (3) ◽  
pp. 712-731 ◽  
Author(s):  
Quan Li ◽  
Guangda Ding ◽  
Ningmei Yang ◽  
Philip John White ◽  
Xiangsheng Ye ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Nitrogen Use Efficiency ◽  
Transcriptome Analysis ◽  
Key Factors ◽  
Brassica Napus L ◽  
Nitrogen Use ◽  
Comparative Genome ◽  
Use Efficiency

scholarly journals Nitrogen Use Efficiency of Local and National Aromatic Rice Varieties in Indonesia

2018 ◽  
Vol 5 (3) ◽  
pp. 79-88
Author(s):  
Chairunnisak Chairunnisak ◽  
Sugiyanta Sugiyanta ◽  
Edi Santosa
Keyword(s):  
Nitrogen Use Efficiency ◽  
N Uptake ◽  
Aromatic Rice ◽  
Rice Varieties ◽  
Nitrogen Use ◽  
N Content ◽  
Low Input ◽  
Plot Yield ◽  
Use Efficiency ◽  
Sampling Plot

Nitrogen use efficiency (NUE) is a necessitate in order to enhance sustainable rice farming in Indonesia. Thus, objective of present research was to evaluate NUE of local and national Indonesian superior aromatic rice treated with different levels of nitrogen fertilizer (N). Planting plot was arranged using five levels of N as the main plot, i.e; 0, 45, 90, 135 and 180 kg ha-1; and two rice varieties as subplot, i.e: Sigupai Abdya (local) and Inpari 23 Bantul (national). The results showed application 180 kg N ha-1 to Sigupai  Abdya significantly increased the plant height. However, it also postponed the flowering time. Inpari 23 Bantul treated with 180 kg N ha-1 produced the highest number of tillers. Combination of 90 kg N ha-1 with Sigupai Abdya variety significantly reduced the number of empty grains. Sigupai Abdya variety has a higher number of grains per panicle and sampling plot yield than Inpari 23 Bantul, and  dosage 90 kg N ha-1 increases grain yield per clump also sampling plot yield significantly. Nitrogen at 180 kg N ha-1 made Sigupai Abdya variety has high N content and absorption N in primordia phase, and the Inpari 23 Bantul variety had grain with high N content. Nitrogen at 90 kg ha-1 caused Sigupai Abdya variety at primordia phase had NUE higher than Inpari 23 Bantul. This study showed that local variety Sigupai Abdya is suitable for development as rice with low input NKeywords: Aceh aromatic, low input, nitrogen dose, N uptake, Oryza sativa L. 

scholarly journals Evaluation of N Efficiency Using Prilled Urea and Urea Super Granules in T. Aman Rice

2015 ◽  
Vol 18 (1) ◽  
pp. 59-63 ◽  
Author(s):  
J Hussain ◽  
M Salim ◽  
MA Siddique ◽  
M Khatun ◽  
S Islam
Keyword(s):  
Nitrogen Use Efficiency ◽  
Rice Varieties ◽  
Marginal Rate ◽  
Nitrogen Use ◽  
Prilled Urea ◽  
N Efficiency ◽  
Use Efficiency ◽  
Marginal Rate Of Return ◽  
Partial Factor Productivity ◽  
Partial Factor

An experiment was conducted at the Experimental field of Department of Agronomy, Bangladesh Agricultural University, Mymensingh, during June to December 2007 to evaluate the nitrogen use efficiency of transplant aman rice using prilled urea (PU) and urea super granule (USG). Three rice varieties (BR11, BRRI dhan30 and BRRI dhan39) were compared at three doses of nitrogen, i.e., PU@125 kg N ha-1, USG @170 kg N ha-1 and USG @ 70 kg N ha-1 in a Randomized Complete Block (RCB) design with three replications. USG each weighing 1 g and 2.6 g were placed manually at the depth of 8-10 cm in the middle of four hills in alternate rows at 12 days after transplanting and PU applied in split applications. The rice var. BR11 at USG @70 kg N ha-1 has produced the maximum grain yield (4.75 t ha-1) which was statistically identical to BRRI dhan30 at USG @ 70 kg N ha-1 (1 g pellet-1). The highest nitrogen use efficiency, partial factor productivity and marginal rate of return (1966%) were recorded from the treatment of BR11 at USG @ 70 kg N ha-1.Bangladesh Agron. J. 2015, 18(1): 59-63

scholarly journals Morphological Physiological and Transcriptional Response to Low Nitrogen Stress in Populus Deltoides Marsh. Clones With Contrasting Nitrogen Use Efficiency

2021 ◽  
Author(s):  
Cun Chen ◽  
Yanguang Chu ◽  
Qinjun Huang ◽  
Weixi Zhang ◽  
Changjun Ding ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Molecular Mechanism ◽  
Nitrogen Use Efficiency ◽  
Time Course ◽  
Populus Deltoides ◽  
Hub Genes ◽  
Nitrogen Use ◽  
Plant Signal Transduction ◽  
Use Efficiency ◽  
N Starvation

Abstract Background: Nitrogen (N) is one of the main factors limiting the wood yield in poplar cultivation. Understanding the molecular mechanism of N utilization could play a guiding role in improving the nitrogen use efficiency (NUE). Results: In this study, three N-efficient genotypes (A) and three N-inefficient genotypes (C) of Populus deltoides were cultured under low N stress (5 μM NH4NO3) and normal N supply (750 μM NH4NO3). The dry matter mass, leaf morphology, and chlorophyll content of both genotypes decreased under N starvation. Interestingly, N starvation induced fine root growth in A, but not in C. Next, a detailed time-course analysis of enzyme activities and gene expression in leaves identified 2,062 differentially expressed genes (DEGs) in A and 1,118 in C, most of which were up-regulated. Moreover, the sensitivity to N starvation of A was weak, and DEGs related to hormone signal transduction played an important role in the low N response in A. The weighted gene co-expression network analysis identified genes related to membrane, catalytic activity, enzymatic activity, and response to stresses might be critical for poplar’s adaption to N starvation and these genes participated in the negative regulation of various biological processes. Finally, ten influential hub genes and twelve transcription factors were identified in the response to N starvation, among them Podel.19G001200, Podel.19G035300, Podel.02G021400, and Podel.04G076900 were related to programmed cell death, and the defense response, and PodelWRKY41, PodelWRKY75, PodelWRKY18, PodelBHLH25, PodelBHLH30, PodelBHLH, and PodelHY5 were involved in plant signal transduction.Conclusions: Under the condition of N starvation, A showed stronger adaptability and a better NUE than C in morphology and physiology. The discovery of hub genes and TFs provided a new information for the analysis of the molecular mechanism of N efficient utilization and the improvement of NUE of poplar.

scholarly journals Genome-wide associated study identifies NAC42-activated nitrate transporter conferring high nitrogen use efficiency in rice

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Weijie Tang ◽  
Jian Ye ◽  
Xiangmei Yao ◽  
Pingzhi Zhao ◽  
Wei Xuan ◽  
...  
Keyword(s):  
Nitrogen Use Efficiency ◽  
Negative Impact ◽  
Nitrate Transporter ◽  
High Nitrogen ◽  
Rice Varieties ◽  
Nitrogen Use ◽  
Genome Wide ◽  
Related Transcription Factor ◽  
Use Efficiency ◽  
Natural Variations

AbstractOver-application of nitrogen fertilizer in fields has had a negative impact on both environment and human health. Domesticated rice varieties with high nitrogen use efficiency (NUE) reduce fertilizer for sustainable agriculture. Here, we perform genome-wide association analysis of a diverse rice population displaying extreme nitrogen-related phenotypes over three successive years in the field, and identify an elite haplotype of nitrate transporter OsNPF6.1HapB that enhances nitrate uptake and confers high NUE by increasing yield under low nitrogen supply. OsNPF6.1HapB differs in both the protein and promoter element with natural variations, which are differentially trans-activated by OsNAC42, a NUE-related transcription factor. The rare natural allele OsNPF6.1HapB, derived from variation in wild rice and selected for enhancing both NUE and yield, has been lost in 90.3% of rice varieties due to the increased application of fertilizer. Our discovery highlights this NAC42-NPF6.1 signaling cascade as a strategy for high NUE and yield breeding in rice.

scholarly journals Identification of High Nitrogen Use Efficiency Phenotype in Rice (Oryza sativa L.) Through Entire Growth Duration by Unmanned Aerial Vehicle Multispectral Imagery

2021 ◽  
Vol 12 ◽  
Author(s):  
Ting Liang ◽  
Bo Duan ◽  
Xiaoyun Luo ◽  
Yi Ma ◽  
Zhengqing Yuan ◽  
...  
Keyword(s):  
Unmanned Aerial Vehicle ◽  
High Throughput ◽  
Nitrogen Use Efficiency ◽  
Developmental Stages ◽  
High Nitrogen ◽  
Growth Duration ◽  
Rice Varieties ◽  
Nitrogen Use ◽  
Aerial Vehicle ◽  
Use Efficiency

Identification of high Nitrogen Use Efficiency (NUE) phenotypes has been a long-standing challenge in breeding rice and sustainable agriculture to reduce the costs of nitrogen (N) fertilizers. There are two main challenges: (1) high NUE genetic sources are biologically scarce and (2) on the technical side, few easy, non-destructive, and reliable methodologies are available to evaluate plant N variations through the entire growth duration (GD). To overcome the challenges, we captured a unique higher NUE phenotype in rice as a dynamic time-series N variation curve through the entire GD analysis by canopy reflectance data collected by Unmanned Aerial Vehicle Remote Sensing Platform (UAV-RSP) for the first time. LY9348 was a high NUE rice variety with high Nitrogen Uptake Efficiency (NUpE) and high Nitrogen Utilization Efficiency (NUtE) shown in nitrogen dosage field analysis. Its canopy nitrogen content (CNC) was analyzed by the high-throughput UAV-RSP to screen two mixed categories (51 versus 42 varieties) selected from representative higher NUE indica rice collections. Five Vegetation Indices (VIs) were compared, and the Normalized Difference Red Edge Index (NDRE) showed the highest correlation with CNC (r = 0.80). Six key developmental stages of rice varieties were compared from transplantation to maturation, and the high NUE phenotype of LY9348 was shown as a dynamic N accumulation curve, where it was moderately high during the vegetative developmental stages but considerably higher in the reproductive developmental stages with a slower reduction rate. CNC curves of different rice varieties were analyzed to construct two non-linear regression models between N% or N% × leaf area index (LAI) with NDRE separately. Both models could determine the specific phenotype with the coefficient of determination (R2) above 0.61 (Model I) and 0.86 (Model II). Parameters influencing the correlation accuracy between NDRE and N% were found to be better by removing the tillering stage data, separating the short and long GD varieties for the analysis and adding canopy structures, such as LAI, into consideration. The high NUE phenotype of LY9348 could be traced and reidentified across different years, locations, and genetic germplasm groups. Therefore, an effective and reliable high-throughput method was proposed for assisting the selection of the high NUE breeding phenotype.

scholarly journals Morphological, physiological, and transcriptional responses to low nitrogen stress in Populus deltoides Marsh. clones with contrasting nitrogen use efficiency

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Cun Chen ◽  
Yanguang Chu ◽  
Qinjun Huang ◽  
Weixi Zhang ◽  
Changjun Ding ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Transcription Factors ◽  
Nitrogen Use Efficiency ◽  
Populus Deltoides ◽  
Hub Genes ◽  
Nitrogen Use ◽  
Group A ◽  
Use Efficiency ◽  
N Starvation ◽  
Low Nitrogen

Abstract Background Nitrogen (N) is one of the main factors limiting the wood yield in poplar cultivation. Understanding the molecular mechanism of N utilization could play a guiding role in improving the nitrogen use efficiency (NUE) of poplar. Results In this study, three N-efficient genotypes (A1-A3) and three N-inefficient genotypes (C1-C3) of Populus deltoides were cultured under low N stress (5 μM NH4NO3) and normal N supply (750 μM NH4NO3). The dry matter mass, leaf morphology, and chlorophyll content of both genotypes decreased under N starvation. The low nitrogen adaptation coefficients of the leaves and stems biomass of group A were significantly higher than those of group C (p < 0.05). Interestingly, N starvation induced fine root growth in group A, but not in group C. Next, a detailed time-course analysis of enzyme activities and gene expression in leaves identified 2062 specifically differentially expressed genes (DEGs) in group A and 1118 in group C. Moreover, the sensitivity to N starvation of group A was weak, and DEGs related to hormone signal transduction and stimulus response played an important role in the low N response this group. Weighted gene co-expression network analysis identified genes related to membranes, catalytic activity, enzymatic activity, and response to stresses that might be critical for poplar’s adaption to N starvation and these genes participated in the negative regulation of various biological processes. Finally, ten influential hub genes and twelve transcription factors were identified in the response to N starvation. Among them, four hub genes were related to programmed cell death and the defense response, and PodelWRKY18, with high connectivity, was involved in plant signal transduction. The expression of hub genes increased gradually with the extension of low N stress time, and the expression changes in group A were more obvious than those in group C. Conclusions Under N starvation, group A showed stronger adaptability and better NUE than group C in terms of morphology and physiology. The discovery of hub genes and transcription factors might provide new information for the analysis of the molecular mechanism of NUE and its improvement in poplar.

scholarly journals OsATG8c-Mediated Increased Autophagy Regulates the Yield and Nitrogen Use Efficiency in Rice

2019 ◽  
Vol 20 (19) ◽  
pp. 4956 ◽  
Author(s):  
Xiaoxi Zhen ◽  
Xin Li ◽  
Jinlei Yu ◽  
Fan Xu
Keyword(s):  
Nitrogen Use Efficiency ◽  
Transcriptional Level ◽  
Nitrogen Use ◽  
Nitrogen Uptake Efficiency ◽  
Yield And Quality ◽  
Autophagy Gene ◽  
N Remobilization ◽  
Use Efficiency ◽  
N Starvation ◽  
Cellular Components

Autophagy, a conserved pathway in eukaryotes, degrades and recycles cellular components, thus playing an important role in nitrogen (N) remobilization. N plays an important role in the growth and development of plants, which also affects plant yield and quality. In this research, it was found that the transcriptional level of a core autophagy gene of rice (Oryza sativa), OsATG8c, was increased during N starvation conditions. It was found that the overexpression of OsATG8c significantly enhanced the activity of autophagy and that the number of autophagosomes, dwarfed the plant height and increased the effective tillers’ number and yield. The nitrogen uptake efficiency (NUpE) and nitrogen use efficiency (NUE) significantly increased in the transgenic rice under both optimal and suboptimal N conditions. Based on our results, OsATG8c is considered to be a good candidate gene for increasing NUE, especially under suboptimal field conditions.

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