scholarly journals Hydropriming and Biopriming Improve Medicago truncatula Seed Germination and Upregulate DNA Repair and Antioxidant Genes

Genes ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 242
Author(s):  
Chiara Forti ◽  
Ajay Shankar ◽  
Anjali Singh ◽  
Alma Balestrazzi ◽  
Vishal Prasad ◽  
...  
Keyword(s):  
Seed Germination ◽  
Medicago Truncatula ◽  
Seed Quality ◽  
Agricultural Soils ◽  
Agricultural Practices ◽  
Seed Priming ◽  
Germination Percentage ◽  
Change Scenario ◽  
Antioxidant Genes ◽  
Northern India

Seed germination is a critical parameter for the successful development of sustainable agricultural practices. While seed germination is impaired by environmental constraints emerging from the climate change scenario, several types of simple procedures, known as priming, can be used to enhance it. Seed priming is defined as the process of regulating seed germination by managing a series of parameters during the initial stages of germination. Hydropriming is a highly accessible and economic technique that involves soaking of seeds in water followed by drying. Biopriming refers to the inoculation of seeds with beneficial microorganism. The present study aims to investigate whether hydropriming and biopriming could enhance seed germination. Thereby, the germination of Medicago truncatula seeds exposed to hydropriming and/or Bacillus spp. isolates was monitored for two-weeks. The seeds were sown in trays containing two types of in situ agricultural soils collected from Northern India (Karsara, Varanasi). This region is believed to be contaminated by solid waste from a nearby power plant. Phenotypic parameters had been monitored and compared to find the most appropriate combination of treatments. Additionally, qRT-PCR was used to evaluate the expression levels of specific genes used as molecular indicators of seed quality. The results show that, while hydropriming significantly enhanced seed germination percentage, biopriming resulted in improved seedling development, represented by increased biomass rather than seedling length. At a molecular level, this is reflected by the upregulation of genes involved in DNA damage repair and antioxidant defence. In conclusion, hydropriming and biopriming are efficient to improve seed germination and seedling establishment in soils collected from damaged sites of Northern India; this is reflected by morphological parameters and molecular hallmarks of seed quality.

Dispersal and germination syndromes of tree seeds in a monsoonal forest in northern India

2005 ◽  
Vol 15 (1) ◽  
pp. 29-42 ◽  
Author(s):  
R.C. Thapliyal ◽  
S.S. Phartyal
Keyword(s):  
Seed Germination ◽  
Seed Storage ◽  
Germination Percentage ◽  
Cold Season ◽  
Poor Quality ◽  
Northern India ◽  
High Germination ◽  
Monsoon Forest ◽  
Mean Germination Time ◽  
Pre Treatment

This paper describes the dispersal–germination characteristics of seeds of 77 native tree species in a seasonal monsoon forest in Uttaranchal state, northern India. Results indicate that 50% of the species dispersed in the hot, dry summer months, 18% during the rainy season, 23% during the cold season and the remainder in late spring. Germination tests on fresh and laboratory-stored seeds revealed a relationship between morphological features of the fruit and both germination percentage and mean germination time (MGT). Highest mean germination (50%) was for dry-dehiscent fruits with winged wind-dispersed seeds, followed by dry-dehiscent fruits with non-winged seeds (38%) and seeds of dry-indehiscent fruits (37%). Lowest germination (29%) was for seeds from fleshy or pulpy fruits. MGT followed the reverse course. Germination data for seeds stored dry in the laboratory during one seeding cycle indicated six patterns of seed germination: (1) average germination percentage of fresh seeds lower than that of stored seeds, indicating an after-ripening requirement; (2) initial high germination percentage followed by low values, indicating a steep to moderate decline in viability following harvest; (3) no germination after 1 or 2 months of seed storage, due to complete loss of viability, indicating short seed longevity; (4) fresh seed germination in some species equalled the average germination value of stored seeds, indicating constant germination for one whole seeding cycle; (5) germination of both fresh and stored seeds remained consistently low throughout the season, indicating a requirement for some kind of pre-treatment or having poor quality of seeds; (6) initial high germination followed by decline and again increase, showing a seasonal rhythm of germination.

scholarly journals Impact of seed size on seeds viability, vigor and storability of Hordeum vulgare (L.)

2018 ◽  
Author(s):  
M. Massimi
Keyword(s):  
Seed Germination ◽  
Seed Size ◽  
Seed Quality ◽  
Accelerated Aging ◽  
Dry Weight ◽  
Germination Percentage ◽  
Seedling Vigor ◽  
Hordeum Vulgare L ◽  
Vigor Index ◽  
The Impact

Seed size may influence seed germination, and seedling vigor. Few investigations are available about the effect of seed size on barley seedling vigor in Jordan. The present study was designed to investigate the impact of seed size on germination percentage, seedling dry weight, seedling vigor index, and germination percentage after accelerated aging in barley. Three seed sizes i.e. large, medium, and small (having diameter of > 2.75 millimeter, 2.5 - 2.75 millimeter, and less than 2.5 millimeter, respectively) were tested in the experiment. Seed quality for different seed size categories was evaluated in the laboratory by measuring seed germination, germination after accelerated aging, as well seedling dry weight and vigor index. Results showed significant differences for large seeds in germination percentages, seedling dry weight and vigor index. It may be concluded that large seed size of barley showed best quality.

scholarly journals Two-Hour Magneto-Priming with Static Magnetic Fields Ranging from 65 ± 3 to 505 ± 8 mT Does Not Improve the Germination Percentage of Industrial Hemp Seed at a Sub-Optimal Germination Temperature

J ◽  
2018 ◽  
Vol 1 (1) ◽  
pp. 192-196
Author(s):  
Kathrin Spendier
Keyword(s):  
Seed Germination ◽  
Magnetic Fields ◽  
Cannabis Sativa ◽  
Seed Priming ◽  
Germination Percentage ◽  
Hemp Seed ◽  
Static Magnetic Fields ◽  
Beneficial Effects ◽  
Industrial Hemp ◽  
The U.S

Industrial hemp is a non-psychoactive variety of Cannabis sativa L., i.e., it contains less than 0.3% tetrahydrocannabinols (THC). This crop is one of historical importance in the U.S. as manufacturers seek industrial hemp as a renewable and sustainable resource for a wide variety of consumer and industrial products. To help farmers succeed, agronomic research on industrial hemp is needed. In this trial, investigations were performed to determine whether magneto-priming, a form of seed priming that involves magnetic fields, effects the germination percentage of industrial hemp seed. Beneficial effects of magnetic fields on seedling growths and germination have been reported for many different plant species. Dry industrial hemp seed was exposed to static magnetic fields ranging from 65 ± 3 to 505 ± 8 mT for 2 h prior to seed germination. Germination was performed at 13.6 ± 0.7 °C, a temperature that is representative of the germination temperatures of industrial hemp in the U.S. state of Colorado. Magneto-priming of seed had no statistically significant effect on seed germination percentage.

scholarly journals The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 94
Author(s):  
Iman Mirmazloum ◽  
Attila Kiss ◽  
Éva Erdélyi ◽  
Márta Ladányi ◽  
Éva Zámboriné Németh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Petri Dish ◽  
Seed Priming ◽  
Germination Percentage ◽  
Positive Effects ◽  
Carum Carvi ◽  
Early Seedling ◽  
Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

scholarly journals Alleviate Seed Ageing Effects in Silybum marianum by Application of Hormone Seed Priming

2015 ◽  
Vol 7 (3) ◽  
pp. 316-321
Author(s):  
Seyed Ata SIADAT ◽  
Seyed Amir MOOSAVI ◽  
Mehran SHARAFIZADEH
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Morphological Structure ◽  
Seed Priming ◽  
Silybum Marianum ◽  
Duration Of Treatment ◽  
Seed Vigour ◽  
Germination Characteristics ◽  
Poor Seed ◽  
Seed Ageing

Most of the medicinal plants have seed dormancy or poor seed germination. This is due to their natural compounds or their morphological structure. Existence of such mechanisms makes the seeds able to endure harsh environments, stressful conditions or natural ageing. Different seed enhancement treatments were proposed in order to improve seed germination. In this study, it was examined Silybum marianum seed longevity (0, 48 and 72 hour of ageing) and the response to seed priming when using different concentrations of cytokinin (50, 200, 350 and 500 ppm) and different duration of treatment (8, 12 and 24 hour). Results revealed that ageing could be successfully alleviated using seed priming. Seed ageing significantly affected seed germination and vigour. Application of cytokinin considerably improved seed vigour in aged and non aged seeds. The most effective seed priming treatment was with 500 ppm cytokine, with the duration of 24 hours. In conclusion, it can be recommend the used of plant growth regulators like cytokine, as a good priming agent, to recover losses of seed quality and improve germination characteristics.

Effect of Calcium and Irrigation Treatments on Peanut Yield, Grade and Seed Quality1

1976 ◽  
Vol 3 (2) ◽  
pp. 81-85 ◽  
Author(s):  
F. R. Cox ◽  
G. A. Sullivan ◽  
C. K. Martin
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Interaction Effects ◽  
Germination Percentage ◽  
Yield Response ◽  
Planting Dates ◽  
Yield Increase ◽  
Large Yield ◽  
Irrigation Treatments

Abstract The effect of irrigation and/or Ca (calcium) application on yield, grade and seed quality was studied using Florigiant peanuts planted on three dates. Calcium application resulted in a small, but consistent, yield increase at all planting dates. Irrigation resulted in a large yield response on early planted peanuts and no response on late planted ones. Grade, specifically sound mature kernel percentage, was improved by both Ca and irrigation treatments but the effects were only slightly additive. Similar interaction effects were noted as these treatments increased the germination percentage of the seed produced and decreased the percentage of kernels with the form of damage termed dark plumule. Both treatments also decreased the amount of watery hypocotyl damage and increased the Ca concentration in the seed. Germination was 89 to 94% when the seed Ca level was greater than 420 ppm, but fell linearly to 52% when the seed Ca decreased from 420 to 200 ppm.

scholarly journals Muskmelon seed priming in relation to seed vigor

2004 ◽  
Vol 61 (1) ◽  
pp. 114-117 ◽  
Author(s):  
Warley Marcos Nascimento ◽  
Fernando Antônio Souza de Aragão
Keyword(s):  
Low Temperature ◽  
Interaction Effect ◽  
Cucumis Melo ◽  
Seed Quality ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Cucumis Melo L ◽  
Aged Seeds

A number of important factors may affect seed priming response, including seed quality. Effects of seed vigor on seed priming response were investigated using seed lots of two muskmelon (Cucumis melo L.) cultivars. Seeds of muskmelon, cvs. Mission and Top Net SR were artificially aged at 43°C for 0, 20 and 40 hours. Seeds were primed for six days in darkness at 25°C in KNO3 (0.35 mol L-1) aerated solution. Aged seeds germinated poorly at 17°C. Priming increased germination rate at 17 and 25°C and germination percentage at 17°C. An interaction effect on germination performance between vigor and priming was observed, especially at low temperature. Priming increased germination performance in seeds of low vigor, and the response was cultivar dependent.

Somaclonal variation in seed germination of dallisgrass biotypes

2005 ◽  
Vol 3 (3) ◽  
pp. 414-420 ◽  
Author(s):  
W.D. Pitman ◽  
S.S. Croughan ◽  
J.L. Nash ◽  
B.C. Venuto
Keyword(s):  
Tissue Culture ◽  
Seed Germination ◽  
Somaclonal Variation ◽  
Seed Quality ◽  
Germination Percentage ◽  
Forage Production ◽  
Paspalum Dilatatum ◽  
Pasture Grass ◽  
Plant Persistence ◽  
Agronomic Potential

Dallisgrass (Paspalum dilatatum Poir.) is an important pasture grass on moist fertile sites in warm climates, however, it is limited in use by periodic low seed quality and resulting erratic availability and establishment difficulties. Due to apomictic reproduction, common pentaploid dallisgrass has not been responsive to traditional plant improvement approaches. Recent research has shown potential for some improvement in forage production and plant persistence with apomictic hexaploid varieties of dallisgrass. Tissue-culture regeneration has been reported to produce somaclonal variation in seed germination of common pentaploid dallisgrass and has recently resulted in availability of potentially useful regenerants of hexaploid dallisgrass. Seed of selected plants from both field and greenhouse environments was evaluated in replicated germination trials to determine whether hexaploid tissue-culture regenerants responded similarly to regenerants from common dallisgrass and to determine whether regenerants expressing potential agronomic usefulness as indicated by preliminary observations differed in germination. The eight germination trials included 299 regenerants with 57 of these being duplicate entries among trials. Germination did not differ among sources of regenerants. Within sources germination differed among regenerants with a few individuals exceeding germination percentage of parent lines in one evaluation of greenhouse-produced seed. Individual regenerants with agronomic potential and high percentage seed germination were identified. The results demonstrate that tissue-culture regeneration can be used to produce improved germination in agronomically useful genotypes of apomictic dallisgrass and suggest that such potential may exist for other recalcitrant plant species.

scholarly journals Influence of seed priming on germination characteristics of sorghum (Sorghum bicolor L. Moench)

2021 ◽  
Vol 911 (1) ◽  
pp. 012086
Author(s):  
Ramlah Arief ◽  
Fauziah Koes ◽  
Oom Komalasari ◽  
Fatmawati
Keyword(s):  
Seed Quality ◽  
Germination Rate ◽  
Primary Root ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Seed Vigor ◽  
Germination Characteristics ◽  
Positive Effect

Abstract Seed priming or invigoration is presowing treatments of improving germination aimed to reduce the time from sowing to emergence and improving emergence uniformity. Research on seed priming was conducted Indonesian Cereal Research Institute (ICERI) seed laboratory from January until March 2021 to evaluate the effect of seed priming on sorghum seed vigor. Seed quality parameters includes germination percentages, germination rate, shoot and primary root length, seedling dryweight, Sorghum seed variety of Suri 4 were used as seed material. Results showed that among the priming treatments that give positive effect on germination percentage, germination rate, seedling dry weight were priming treatment with KNO3 1.5%.

scholarly journals Temperature and Light Effects on Germination Behaviour of African Eggplant (Solanum aethiopicum L.) Seeds

2021 ◽  
Author(s):  
H.M. Botey ◽  
J.O. Ochuodho ◽  
L. Ngode ◽  
H. Dwamena ◽  
I. Osei-Tutu
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Light Exposure ◽  
Germination Percentage ◽  
Effect Of Temperature ◽  
Maximum Temperature ◽  
Percentage Germination ◽  
Ambient Conditions ◽  
Solanum Aethiopicum ◽  
Germination Behaviour

Background: A preliminary study of the African eggplant seeds obtained from farmers sources recorded a wide variation in percentage germination under ambient conditions (25±2°C). The germination percentage ranged from 0% to 25%, while fresh seeds ranged between 53% and 87%. As temperature and light are important factors of seed germination, the current study investigated the effect of temperature on the germination pattern and the influence of light interaction with temperature on seed germination of African eggplant (Solanum aethiopicum L.) under controlled conditions. Methods: Seeds of two cultivars of African eggplant were subjected to constant and alternating temperatures and under three light exposure regimes. Seed quality was accessed by per cent germination, mean germination time, time to reach 50% germination, germination index and mean daily germination. Result: The highest percentage germination under constant temperatures was recorded at 25°C (76%) and 20°C (74%). The maximum temperature and light conditions required for maximum seed germination quality (76-95%) at the shortest time (4-5 days) was 30/20°C under alternating 8/16 hours light and dark.

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