scholarly journals Identification the Cross-Reactive or Species-Specific Allergens of Tyrophagus putrescentiae and Development Molecular Diagnostic Kits for Allergic Diseases

Diagnostics ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 665
Author(s):  
Ching-Hsiang Yu ◽  
Jaw-Ji Tsai ◽  
Yi-Hsueh Lin ◽  
Sheng-Jie Yu ◽  
En-Chih Liao
Keyword(s):  
Prediction Accuracy ◽  
Allergic Diseases ◽  
House Dust Mites ◽  
Molecular Diagnostic ◽  
Elderly Subjects ◽  
Recombinant Allergens ◽  
Tyrophagus Putrescentiae ◽  
Mite Allergy ◽  
The Cross ◽  
Species Specific

Mite allergens are considerable factors in the genesis of allergic diseases. The storage mite Tyrophagus putrescentiae (Tp) appears in contaminated foods and household surroundings. The current diagnostic tools for Tp allergy are mostly based on crude extracts and still contain shortcomings. This study aimed to investigate the immunoglobulin E (IgE)- responsiveness profiles of Tp-allergic patients and develop a molecular diagnostic method using recombinant allergens. Allergenic components were characterized as cross-reacting or species-specific allergens, in which the effective combinations of recombinant allergens were developed and analyzed in terms of the prediction accuracy for clinical diagnosis. Seven recombinant allergens were cloned and generated to detect the IgE responsiveness of the Tp allergy. A survey on the prevalence of mite allergy showed there were higher sensitizations with IgE responsiveness to house dust mites (HDM) (78.9–80.9%) than to storage mites Tp (35.6%). Prevalence of sensitization to Tp was higher in elderly subjects. The principal IgE-binding components of Tp were Tyr p 1, Tyr p 2 and Tyr p 3. Prediction accuracy for Tp allergy by IgE-responsiveness combination D (Tyr p 1, Tyr p 2 & Tyr p 3) was with high precision (100%). Avoiding the cross-reactivity of Dermatophagoides pteronyssinus, the prediction accuracy of IgE-responsiveness combination H+ (Tyr p 1, Tyr p 2, Tyr p 3, Tyr p 7, Tyr p 8, Tyr p 10 & Tyr p 20) was suitable for Tp-specific diagnosis. Panels of Tp allergens were generated and developed a diagnostic kit able beneficial to identify IgE-mediated Tp hypersensitivity.

Vocal Learning of Japanese and Rhesus Monkeys

Behaviour ◽  
1989 ◽  
Vol 109 (3-4) ◽  
pp. 191-199 ◽  
Author(s):  
Nobuo Masataka ◽  
Kazuo Fujita
Keyword(s):  
Rhesus Monkeys ◽  
Japanese Monkey ◽  
Species Difference ◽  
Vocal Learning ◽  
The Other ◽  
Playback Experiments ◽  
Biological Mothers ◽  
Japanese Monkeys ◽  
The Cross ◽  
Species Specific

AbstractForaging vocalizations given by Japanese and rhesus momkeys reared by their biological mothers differed from each other in a single parameter. Calls made by a Japanese monkey fostered by a rhesus female were dissimilar to those of conspecifics reared by their biological mothers, but similar to those of rhesus monkeys reared by their biological mothers, and the vocalizations given by rhesus monkeys fostered by Japanese monkey mothers were dissimilar to those of conspecifics reared by their biological mothers, but similar to those of Japanese monkeys reared by their biological mothers. Playback experiments revealed that both Japanese and rhesus monkeys distinguished between the calls of Japanese monkeys reared by their biological mothers and of the cross-fostered rhesus monkeys on one hand, and the vocalizations of rhesus monkeys reared by their biological mothers and of the cross-fostered Japanese monkey on the other hand. Thus, production of species-specific vocalizations was learned by each species, and it was the learned species-difference which the monkeys themselves discriminated.

scholarly journals Molecular identification of the swede midge (Diptera: Cecidomyiidae)

2004 ◽  
Vol 136 (6) ◽  
pp. 771-780 ◽  
Author(s):  
Juerg E. Frey ◽  
Beatrice Frey ◽  
Robert Baur
Keyword(s):  
Large Scale ◽  
Crop Protection ◽  
Adult Life ◽  
Molecular Diagnostic ◽  
Pest Species ◽  
Adult Life Span ◽  
Protection Measures ◽  
Major Pest ◽  
Swede Midge ◽  
Species Specific

AbstractEarly detection of pest infestation is a prerequisite for sustainable crop protection. However, many pest species are difficult to detect and thus infestation is diagnosed from damage observed on the respective crop. This diagnosis is often made too late for implementation of crop protection measures, and serious crop losses may result. The swede midge, Contarinia nasturtii Kieffer, is a major pest of Brassica L. (Brassicaceae) vegetables in Europe that has recently invaded North America. With its small size and short adult life-span, and the cryptic lifestyle of the larvae feeding at the growing points of its host plants, it is usually detected only after damage has already occurred. Furthermore, because field-trapped specimens are rarely fully intact, it is extremely difficult to identify. Therefore, we developed a species-specific molecular diagnostic method that enables reliable identification of swede midge from various sources such as alcohol or sticky glue traps. The method enables large-scale screening of field-trapped specimens and is used to evaluate the attractiveness and specificity of pheromone traps that are currently under development.

scholarly journals Soybean Sudden Death Syndrome Species Diversity Within North and South America Revealed by Multilocus Genotyping

2010 ◽  
Vol 100 (1) ◽  
pp. 58-71 ◽  
Author(s):  
Kerry O'Donnell ◽  
Stacy Sink ◽  
María Mercedes Scandiani ◽  
Alicia Luque ◽  
Analía Colletto ◽  
...  
Keyword(s):  
Sudden Death ◽  
South America ◽  
Sudden Death Syndrome ◽  
Molecular Diagnostic ◽  
Sequence Analyses ◽  
Diagnostic Assays ◽  
Molecular Phylogenetic ◽  
Species Specific ◽  
North And South America ◽  
North And South

Sudden death syndrome (SDS) of soybean has become a serious constraint to the production of this crop in North and South America. Phenotypic and multilocus molecular phylogenetic analyses, as well as pathogenicity experiments, have demonstrated that four morphologically and phylogenetically distinct fusaria can induce soybean SDS. Published molecular diagnostic assays for the detection and identification of these pathogens have reported these pathogens as F. solani, F. solani f. sp. glycines, or F. solani f. sp. phaseoli, primarily because the species limits of these four pathogens were only recently resolved. In light of the recent discovery that soybean SDS and Phaseolus and mung bean root rot (BRR) are caused by four and two distinct species, respectively, multilocus DNA sequence analyses were conducted to assess whether any of the published molecular diagnostic assays were species-specific. Comparative DNA sequence analyses of the soybean SDS and BRR pathogens revealed that highly conserved regions of three loci were used in the design of these assays, and therefore none were species-specific based on our current understanding of species limits within the SDS–BRR clade. Prompted by this finding, we developed a high-throughput multilocus genotyping (MLGT) assay which accurately differentiated the soybean SDS and two closely related Phaseolus and mung BRR pathogens based on nucleotide polymorphism within the nuclear ribosomal intergenic spacer region rDNA and two anonymous intergenic regions designated locus 51 and 96. The single-well diagnostic assay, employing flow cytometry and a novel fluorescent microsphere array, was validated by independent multilocus molecular phylogenetic analysis of a 65 isolate design panel. The MLGT assay was used to reproducibly type a total of 262 soybean SDS and 9 BRR pathogens. The validated MLGT array provides a unique molecular diagnostic for the accurate identification and molecular surveillance of these economically important plant pathogens.

scholarly journals In SilicoPrediction of T and B Cell Epitopes of Der f 25 inDermatophagoides farinae

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Xiaohong Li ◽  
Hai-Wei Yang ◽  
Hao Chen ◽  
Jing Wu ◽  
Yehai Liu ◽  
...  
Keyword(s):  
T Cell ◽  
B Cell ◽  
Triosephosphate Isomerase ◽  
Allergic Diseases ◽  
Major Allergen ◽  
House Dust Mites ◽  
T Cell Epitopes ◽  
B Cell Epitopes ◽  
Physiochemical Properties ◽  
Linear Epitopes

The house dust mites are major sources of indoor allergens for humans, which induce asthma, rhinitis, dermatitis, and other allergic diseases. Der f 25 is a triosephosphate isomerase, representing the major allergen identified inDermatophagoides farinae. The objective of this study was to predict the B and T cell epitopes of Der f 25. In the present study, we analyzed the physiochemical properties, function motifs and domains, and structural-based detailed features of Der f 25 and predicted the B cell linear epitopes of Der f 25 by DNAStar protean system, BPAP, and BepiPred 1.0 server and the T cell epitopes by NetMHCIIpan-3.0 and NetMHCII-2.2. As a result, the sequence and structure analysis identified that Der f 25 belongs to the triosephosphate isomerase family and exhibited a triosephosphate isomerase pattern (PS001371). Eight B cell epitopes (11–18, 30–35, 71–77, 99–107, 132–138, 173–187, 193–197, and 211–224) and five T cell epitopes including 26–34, 38–54, 66–74, 142–151, and 239–247 were predicted in this study. These results can be used to benefit allergen immunotherapies and reduce the frequency of mite allergic reactions.

scholarly journals Innate Immune Responses in House Dust Mite Allergy

ISRN Allergy ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-18 ◽  
Author(s):  
Alain Jacquet
Keyword(s):  
House Dust ◽  
Molecular Model ◽  
Inflammatory Cells ◽  
Specific Ige ◽  
Innate Immune ◽  
Allergic Response ◽  
House Dust Mites ◽  
T Cell Epitopes ◽  
House Dust Mite Allergy ◽  
Mite Allergy

Sensitizations to house dust mites (HDM) trigger strong exacerbated allergen-induced inflammation of the skin and airways mucosa from atopic subjects resulting in atopic dermatitis as well as allergic rhinitis and asthma. Initially, the Th2-biased HDM allergic response was considered to be mediated only by allergen B- and T-cell epitopes to promote allergen-specific IgE production as well as IL-4, IL-5, and IL-13 to recruit inflammatory cells. But this general molecular model of HDM allergenicity must be revisited as a growing literature suggests that stimulations of innate immune activation pathways by HDM allergens offer new answers to the following question: what makes an HDM allergen an allergen? Indeed, HDM is a carrier not only for allergenic proteins but also microbial adjuvant compounds, both of which are able to stimulate innate signaling pathways leading to allergy. This paper will describe the multiple ways used by HDM allergens together with microbial compounds to control the initiation of the allergic response through engagement of innate immunity.

A Molecular Diagnostic Algorithm to Guide Pollen Immunotherapy in Southern Europe: Towards Component-Resolved Management of Allergic Diseases

2013 ◽  
Vol 162 (2) ◽  
pp. 163-172 ◽  
Author(s):  
Nikolaos Douladiris ◽  
Savvas Savvatianos ◽  
Irene Roumpedaki ◽  
Chrysanthi Skevaki ◽  
Dimitrios Mitsias ◽  
...  
Keyword(s):  
Diagnostic Algorithm ◽  
Allergic Diseases ◽  
Molecular Diagnostic ◽  
Southern Europe
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