scholarly journals Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent)

Crystals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 367
Author(s):  
Moamen S. Refat ◽  
Reham Z. Hamza ◽  
Abdel Majid A. Adam ◽  
Hosam A. Saad ◽  
Adil A. Gobouri ◽  
...  
Keyword(s):  
Reproductive Toxicity ◽  
Average Diameter ◽  
Testicular Tissue ◽  
Male Rats ◽  
Flavonoid Compound ◽  
Therapeutic Effects ◽  
Ultrastructural Studies ◽  
Ameliorative Effect ◽  
Anti Inflammatory ◽  
The Brain

The aim of this study was to evaluate the antioxidant and anti-inflammatory effects of the new [Ru(Q)(Cl)2(H2O)2] complex (RuIII/Q). A new vital complex containing quercetin flavonoid compound (Q) with ruthenium (III) ions was synthesized. The molar conductivity of the RuIII/Q complex was measured in dimethylsulfoxide (DMSO) with value 12 (Ω−1 mol−1 cm−1, indicating their non-electrolytic nature. Infrared (FTIR) spectroscopic investigation of the RuIII/Q complex indicated that Q is coordinated as a bidentate with Ru metal ions through the oxygen of carbonyl C(4)=O group and oxygen of phenolic C(3)−O group based on the wavenumber shifts at 1654 and 1335 cm−1 respectively. The electronic (UV−Vis) spectra and the magnetic susceptibility value (1.85 B.M.) revealed that the Ru(III) complex has an octahedral geometry. The average diameter of the RuIII/Q nanoparticles was approximately 7–15 nm according to the transmission electron microscopy. The thermogravimetric study (TG/DTG) indicates that the RuIII/Q compound is quite stable until 300 °C. To assess biological activity, 60 male rats were allocated to six groups, namely control, DG (D-galactose), Q, RuIII/Q, DG plus Q, and DG plus RuIII/Q. Antioxidant enzymes (SOD, CAT, GPx, and GRx), markers of lipid peroxidation (such as MDA), expression of genes (namely Nrf2, Cu-ZnSOD, CAT, GPx, cyto c, P53, Bax, BCl2, caspase-3, and caspase-9 in testicular tissue), glutamate, 4-hydroxynonenal (HNE), GSH, HCY, amyloid beta, and GABA levels were evaluated in brain tissues. Cytokines, such as IL-6 and TNF-α, histological and ultrastructural studies were estimated in both the brain and testicular tissues, while the comet assay was performed in the brain tissue. RuIII/Q administration either alone or combined with DG reduced oxidative injury to normal levels and decreased apoptotic activities. Thus, RuIII/Q inhibited injury in both the testis and brain and reduced oxidative stress in male rats. The (RuIII/Q) complex has a potent ameliorative effect against aging neurotoxicity, reproductive toxicity, and antihepatic cancer activity induced by D-galactose (DG).

Ameliorative effect of Alpinia officinarum Hance extract on nonylphenol‐induced reproductive toxicity in male rats

Andrologia ◽  
2021 ◽  
Author(s):  
Marzieh Pirzadeh ◽  
Mohammad Barary ◽  
Seyed Mohammad Hosseini ◽  
Sohrab Kazemi ◽  
Ali Akbar Moghadamnia
Keyword(s):  
Reproductive Toxicity ◽  
Male Rats ◽  
Alpinia Officinarum ◽  
Ameliorative Effect

Abstract WP491: HUCBC Treatment Promotes White Matter Remodeling and Improves Glymphatic Function and Cognitive Outcome in Rats With Vascular Dementia

Stroke ◽  
2020 ◽  
Vol 51 (Suppl_1) ◽  
Author(s):  
Poornima Venkat ◽  
Alex Zacharek ◽  
Michael Chopp ◽  
Jieli Chen
Keyword(s):  
White Matter ◽  
Vascular Dementia ◽  
Cognitive Outcome ◽  
Discrimination Index ◽  
Male Rats ◽  
Morris Water Maze Test ◽  
Therapeutic Effects ◽  
Term Memory ◽  
Test Discrimination ◽  
The Brain

Aim: Vascular Dementia (VaD) accounts for nearly 20% of all dementia. We have investigated the therapeutic effects of human umbilical cord blood cells (HUCBCs) treatment of VaD and tested mechanisms such as that white matter (WM) remodeling and glymphatic function that contribute to improving cognitive outcome. Methods: Male rats (6-8m old) were subjected to a multiple microinfarct model (MMI, 500-800 cholesterol crystals injected into the internal carotid artery) of VaD, and 3 days later treated with PBS or HUCBC (5х10 6 , i.v.) n=6/group. Cognitive tests were conducted and rats sacrificed at 28 days after MMI. To evaluate glymphatic function, fluorescent tracers (Texas Red dextran, MW: 3 kD and FITC-dextran, MW: 500 kD) were injected into the cisterna magna over 30min at 14 days after MMI. Rats (6/group/time point) were sacrificed at 30min, 3h, and 6h and tracer movement analyzed using laser scanning confocal microscopy. Results: HUCBC treatment significantly improves short term memory (Novel object recognition test discrimination index: MMI: 35.6±3.6%; MMI+HUCBC: 74.2±4.3%; p<0.0001), long term memory (odor test discrimination index: MMI: 49.9±5.8%; MMI+HUCBC: 69.2±4.8%; p<0.01) and spatial learning and memory (Morris water maze test: decreased latency and increased % of time in target quadrant, p<0.05) compared to control MMI rats. HUCBC treatment significantly increases axon and myelin density, increases oligodendrocyte and oligodendrocyte progenitor cells number, and increases Synaptophysin expression in the brain compared to control MMI rats. HUCBC treatment of MMI in rats significantly improves glymphatic function by reversing MMI induced delay in cerebrospinal fluid penetration into the brain parenchyma via paravascular pathways as well as delayed waste clearance from the brain. HUCBC treatment significantly decreases serum protein expression of BACE1, S100, MCP-1, and TGF-β which may contribute to HUCBC induced therapeutic effects. Conclusions: HUCBC treatment of an MMI rat model of VaD promotes WM remodeling, anti-inflammatory effects and improves glymphatic function which in concert may contribute to the improvement in cognition and memory. Thus, HUCBC treatment warrants further investigation as a potential therapy for VaD.

scholarly journals Taurine reverses sodium fluoride-mediated increase in inflammation, caspase-3 activity, and oxidative damage along the brain–pituitary–gonadal axis in male rats

2017 ◽  
Vol 95 (9) ◽  
pp. 1019-1029 ◽  
Author(s):  
Isaac A. Adedara ◽  
Bolanle F. Olabiyi ◽  
TeminiJesu D. Ojuade ◽  
Umar F. Idris ◽  
Esther M. Onibiyo ◽  
...  
Keyword(s):  
Sodium Fluoride ◽  
Caspase 3 ◽  
Reproductive Toxicity ◽  
Male Rats ◽  
Reproductive Dysfunction ◽  
Functional Changes ◽  
Histological Damage ◽  
Significant Reversal ◽  
Antioxidant Enzymes Activities ◽  
The Brain

Excessive exposure to fluoride is associated with male reproductive dysfunction in humans and animals. Taurine (2-aminoethane sulfonic acid) is a free intracellular β-amino acid with antioxidant, anti-inflammatory, and neuroprotective properties. However, the effect of taurine on fluoride-induced reproductive toxicity has not been reported. The present study investigated the influence of taurine on sodium fluoride (NaF)-induced functional changes along the brain–pituitary–gonadal axis in male rats. NaF was administered singly in drinking water at 15 mg·L−1 alone or orally co-administered by gavage with taurine at 100 and 200 mg·(kg body mass)−1 for 45 consecutive days. Results showed that taurine significantly prevented NaF-induced increase in oxidative stress indices as well as augmented antioxidant enzymes activities and glutathione level in the brain, testes, and epididymis of the treated rats. Moreover, taurine reversed NaF-induced elevation in inflammatory biomarkers and caspase-3 activity as well as histological damage in the brain, testes, and epididymis of the treated rats. The significant reversal of NaF-induced decreases in testosterone level and testicular activities of acid phosphatase, alkaline phosphatase, and lactate dehydrogenase by taurine was accompanied by enhancement of sperm functional characteristics in the treated rats. Taurine may be a possible chemopreventive candidate against reproductive dysfunction resulting from fluoride exposure.

scholarly journals Potent Antiarthritic Properties of Phloretin in Murine Collagen-Induced Arthritis

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Shun-Ping Wang ◽  
Shih-Chao Lin ◽  
Shiming Li ◽  
Ya-Hsuan Chao ◽  
Guang-Yuh Hwang ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Bone Destruction ◽  
Joint Inflammation ◽  
Flavonoid Compound ◽  
Therapeutic Effects ◽  
Potential Candidate ◽  
Collagen Induced Arthritis ◽  
Hind Limbs ◽  
Collagen Antibodies ◽  
Anti Inflammatory

In the exploration of potential therapeutic agents for rheumatoid arthritis (RA), DBA/1J mice are used as the RA model of collagen-induced arthritis (CIA). Phloretin, a flavonoid compound extracted fromPrunus mandshurica, has been found to exhibit anti-inflammatory activity, making it a potential candidate for treatment of RA. The objective of this study was to evaluate the therapeutic effects of phloretin on CIA mice. CIA mice were dosed daily with phloretin at either 50 or 100 mg/kg among two treatment groups. CIA treated mice showed mitigation of clinical symptoms of RA in addition to reduced inflammation of hind-limbs compared to mice who did not receive phloretin. Histological analysis showed that phloretin suppressed the severity of RA and effectively mitigated joint inflammation and cartilage- and bone-destruction via reducing proinflammatory cytokine productions (TNF-α, IL-6, IL-1β, and IL-17). This was at least partially mediated by causing inadequate splenocyte activation and proliferation. Moreover, phloretin-treated CIA mice showed decreased oxidative stress and diminished levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in paw tissues as well as reduced productivity of anti-collagen antibodies in serum. We have concluded that phloretin could be a potent and effective antiarthritis agent, demonstrating anti-inflammatory, antioxidative, and immunomodulatory effects in CIA mice.

scholarly journals 5-Aminoisoquinolinone, a PARP-1 Inhibitor, Ameliorates Immune Abnormalities through Upregulation of Anti-Inflammatory and Downregulation of Inflammatory Parameters in T Cells of BTBR Mouse Model of Autism

2021 ◽  
Vol 11 (2) ◽  
pp. 249
Author(s):  
Khaled Alhosaini ◽  
Mushtaq A. Ansari ◽  
Ahmed Nadeem ◽  
Saleh A. Bakheet ◽  
Sabry M. Attia ◽  
...  
Keyword(s):  
T Cells ◽  
Autism Spectrum ◽  
Therapeutic Effects ◽  
Inflammatory Parameters ◽  
Patterns Of Behavior ◽  
Mrna And Protein Expression ◽  
Reciprocal Social Interaction ◽  
Anti Inflammatory ◽  
The Brain ◽  
Parp 1

Autism spectrum disorder (ASD) covers a range of neurodevelopmental disorders involving impairments in communication and repetitive and stereotyped patterns of behavior and reciprocal social interaction. 5-Aminoisoquinolinone (5-AIQ), a PARP-1 inhibitor, has neuroprotective and anti-inflammatory effects. We investigated the influence of 5-AIQ-treatment in BTBR T+ Itpr3tf/J (BTBR) mice as an autism model and used flow cytometry to assess the effect of 5-AIQ on FOXP3, Helios, GATA3, IL-9, IL-10 and IL-17A production by CXCR6+ and CD4+ T cells in the spleen. We also confirmed the effect of 5-AIQ treatment on expression of FOXP3, Helios, GATA3, IL-17A, IL-10, and IL-9 mRNA and protein expression levels in the brain tissue by quantitative PCR and western blotting. Our results demonstrated that 5-AIQ-treated BTBR mice had significantly increased numbers of CXCR6+FOXP3+, CXCR6+IL-10+, and CXCR6+Helios+ cells and decreased numbers of CD4+GATA3+, CD4+IL-9+, and CD4+IL-17A+ cells as compared with those in untreated BTBR mice. Our results further demonstrated that treatment with 5-AIQ in BTBR mice increased expression for FOXP3, IL-10, and Helios, and decreased expression for GATA3, IL-17A, and IL-9 mRNA. Our findings support the hypotheses that 5-AIQ has promising novel therapeutic effects on neuroimmune dysfunction in autism and is associated with modulation of Treg and Th17 cells.

scholarly journals Therapeutic effects of astragaloside IV and Astragalus spinosus saponins against bisphenol A-induced neurotoxicity and DNA damage in rats

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11930
Author(s):  
Amina E. Essawy ◽  
Heba-Tallah Abd Elrahim Abd Elkader ◽  
Omaima A. Khamiss ◽  
Saber Mohamed Eweda ◽  
Heba Mohamed Abdou
Keyword(s):  
Dna Damage ◽  
Bisphenol A ◽  
Brain Regions ◽  
Male Rats ◽  
Therapeutic Effects ◽  
Sprague Dawley ◽  
Astragaloside Iv ◽  
Adult Age ◽  
Ameliorative Effect ◽  
Weaning Age

Background Bisphenol A (BPA) is an endocrine disruptor to which humans are often subjected during daily life. This study aimed to investigate the ameliorative effect of astragaloside IV (ASIV) or saponins extracted from Astragalus spinosus (A. spinosus) against DNA damage and neurotoxic effects induced by BPA in prefrontal cortex (PFC), hippocampal and striatal brain regions of developing male rats. Materials and Methods Juvenile PND20 (pre-weaning; age of 20 days) male Sprague Dawley rats were randomly and equally divided into four groups: control, BPA, BPA+ASIV and BPA+A. spinosus saponins groups. Bisphenol A (125 mg/kg/day) was administrated orally to male rats from day 20 (BPA group) and along with ASIV (80 mg/kg/day) (BPA+ASIV group) or A. spinosus saponin (100 mg/kg/day) (BPA+ A. spinosus saponins group) from day 50 to adult age day 117. Results Increased level of nitric oxide (NO) and decreased level of glutamate (Glu), glutamine (Gln), glutaminase (GA) and glutamine synthetase (GS) were observed in the brain regions of BPA treated rats compared with the control. On the other hand, co-administration of ASIV or A. spinosus saponin with BPA considerably improved levels of these neurochemicals. The current study also revealed restoration of the level of brain derived neurotrophic factor (BDNF) and N-methyl-D-aspartate receptors (NR2A and NR2B) gene expression in BPA+ ASIV and BPA+A. spinosus saponins groups. The co-treatment of BPA group with ASIV or A. spinosus saponin significantly reduced the values of comet parameters as well as the intensity of estrogen receptors (ERs) immunoreactive cells and improved the histological alterations induced by BPA in different brain regions. Conclusion It could be concluded that ASIV or A. spinosus saponins has a promising role in modulating the neurotoxicity and DNA damage elicited by BPA.

scholarly journals Therapeutic Effects of Oligonol, Acupuncture, and Quantum Light Therapy in Chronic Nonbacterial Prostatitis

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
İlhan Öztekin ◽  
Hakan Akdere ◽  
Nuray Can ◽  
Tevfik Aktoz ◽  
Ersan Arda ◽  
...  
Keyword(s):  
Chronic Prostatitis ◽  
Light Therapy ◽  
Male Rats ◽  
Treatment Modalities ◽  
Albino Rats ◽  
Therapeutic Effects ◽  
Group I ◽  
Chronic Nonbacterial Prostatitis ◽  
Nonbacterial Prostatitis ◽  
Anti Inflammatory

This research aimed to compare anti-inflammatory effects of oligonol, acupuncture, and quantum light therapy in rat models of estrogen-induced prostatitis. Adult male Wistar albino rats were grouped as follows: Group I, control (n= 10); Group II, chronic prostatitis (n= 10); Group III, oligonol (n= 10); Group IV, acupuncture (n= 10); Group V, quantum (n= 10); Group VI, oligonol plus quantum (n= 10); Group VII, acupuncture plus oligonol (n= 10); Group VIII, quantum plus acupuncture (n= 10); and Group IX, acupuncture plus quantum plus oligonol (n= 10). Chronic prostatitis (CP) was induced by the administration of 17-beta-estradiol (E2) and dihydrotestosterone (DHT). Oligonol was given for 6 weeks at a dose of 60 mg/day. Acupuncture needles were inserted at CV 3/4 and bilaterally B 32/35 points with 1-hour manual stimulation. Quantum therapy was administered in 5-minute sessions three times weekly for 6 weeks. Lateral lobes of prostates were dissected for histopathologic evaluation. Although all of the treatment modalities tested in this study showed anti-inflammatory effects in the treatment of CP in male rats, a synergistic effect was observed for oligonol plus quantum light combination. Monotherapy with oligonol showed a superior anti-inflammatory efficacy as compared to quantum light and acupuncture monotherapies.

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