scholarly journals Sustainable Effects of Human Dental Pulp Stem Cell Transplantation on Diabetic Polyneuropathy in Streptozotocine-Induced Type 1 Diabetes Model Mice

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2473
Author(s):  
Masaki Hata ◽  
Maiko Omi ◽  
Yasuko Kobayashi ◽  
Nobuhisa Nakamura ◽  
Megumi Miyabe ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Diabetic Polyneuropathy ◽  
Dorsal Root Ganglion Neurons ◽  
Therapeutic Effects ◽  
Long Term Effects ◽  
Post Transplantation ◽  
Diabetes Model ◽  
Human Dental Pulp ◽  
Ganglion Neurons

Dental pulp stem cells (DPSCs) are suitable for use in regenerative medicine. Cryopreserved human DPSCs (hDPSCs) ameliorate diabetic polyneuropathy, and the effects of hDPSC transplantation are related to VEGF and NGF secretion. This study evaluated the long-term effects of a single transplantation of hDPSCs on diabetic polyneuropathy. hDPSCs were obtained from human third molars extracted for orthodontic treatment, which were then transplanted into the unilateral hindlimb skeletal muscles 8 weeks after streptozotocin injection in nude mice. The effects of hDPSC transplantation were analyzed at 16 weeks post-transplantation. DPSC transplantation significantly improved delayed nerve conduction velocity, decreased blood flow, and increased sensory perception thresholds. Furthermore, the hDPSC-conditioned medium promoted the neurite outgrowth of dorsal root ganglion neurons. In conclusion, the therapeutic effects of hDPSC transplantation with a single injection last for prolonged periods and may be beneficial in treating long-term diabetic polyneuropathy.

scholarly journals Harvesting human dental pulp stem cells using long term ex-plant culture

2019 ◽  
Vol 8 (1) ◽  
pp. 1-2
Author(s):  
Vikrant R Patil ◽  
Avinash H Kharat ◽  
Supriya Kheur ◽  
Mahesh Chavan ◽  
D Gopalakrishnan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Plant Culture ◽  
Human Dental Pulp

scholarly journals XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4/Sox2/c-Myc

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Lu Liu ◽  
Zhengjun Peng ◽  
Zhezhen Xu ◽  
Xi Wei
Keyword(s):  
Western Blot ◽  
Real Time ◽  
Real Time Pcr ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Multilineage Differentiation ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

Introduction. Xeroderma pigmentosum group C (XPC), essential component of multisubunit stem cell coactivator complex (SCC), functions as the critical factor modulating pluripotency and genome integrity through interaction with Oct-4/Sox2. However, its specific role in regulating pluripotency and multilineage differentiation of human dental pulp cells (DPCs) remains unknown. Methods. To elucidate the functional role XPC played in pluripotency and multilineage differentiation of DPCs, expressions of XPC in DPCs with long-term culture were examined by real-time PCR and western blot. DPCs were transfected with lentiviral-mediated human XPC gene; then transfection rate was investigated by real-time PCR and western blot. Cell cycle, apoptosis, proliferation, senescence, multilineage differentiation, and expression of Oct-4/Sox2/c-Myc in transfected DPCs were examined. Results. XPC, Oct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture. XPC genes were upregulated in DPCs at P2 after transfection and maintained high expression level at P3 and P7. Cell proliferation, PI value, and telomerase activity were enhanced, whereas apoptosis was suppressed in transfected DPCs. Oct-4/Sox2/c-Myc were significantly upregulated, and multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection. Conclusions. XPC plays an essential role in the modulation of pluripotency and multilineage differentiation of DPCs through regulation of Oct-4/Sox2/c-Myc.

Expression of MMP-13 (collagenase-3) in long-term cultures of human dental pulp cells

2008 ◽  
Vol 53 (8) ◽  
pp. 791-799 ◽  
Author(s):  
Lokesh Suri ◽  
Petros D. Damoulis ◽  
Trang Le ◽  
Eleni Gagari
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

Long-Term Effects of Middle Ear Tendon Resection on Middle Ear Myoclonic Tinnitus, Hearing, and Hyperacusis

2017 ◽  
Vol 22 (6) ◽  
pp. 343-349 ◽  
Author(s):  
Dong-Kee Kim ◽  
Jung Mee  Park ◽  
Jung Ju Han ◽  
Yun Min Chung ◽  
Jung Min Kim ◽  
...  
Keyword(s):  
Middle Ear ◽  
Complete Recovery ◽  
Stress Index ◽  
Therapeutic Effects ◽  
Long Term Effects ◽  
Vas Scores ◽  
The Mean ◽  
High Control

Objective: To evaluate the therapeutic effects of middle ear tendon resection (METR) on middle ear myoclonic tinnitus (MEMT) and to investigate its long-term effects on hearing and hyperacusis. Materials and Methods: Thirty-seven patients with MEMT with a mean age of 33.2 ± 11.8 years were included in this study. METR was performed on all 37 MEMT patients (41 ears) between November 2004 and August 2016. The mean follow-up period was 16.1 months. We examined changes in tinnitus and accompanying stress and depression in patients after surgery, and examined the hearing changes and the occurrence of complications including hyperacusis. Results: After surgery, 34 (91.9%) patients exhibited complete resolution of MEMT during their follow-up period, and 3 patients showed a partial response. The mean Visual Analog Scale (VAS) scores for tinnitus severity, the Tinnitus Handicap Inventory (THI), and stress index decreased significantly after surgery (p < 0.05, paired t test). No patient developed hearing loss or hyperacusis following surgery. Preexisting hyperacusis even improved in most of the patients with intractable MEMT after surgery. Recurrence of the symptom occurred in only 1 patient, who underwent revision surgery with improvement. We observed 1 case of postoperative delayed facial palsy with complete recovery in 2 weeks. Conclusions: METR seems to be an effective and safe treatment option for intractable MEMT, considering its high control rate of tinnitus and no long-term harmful effects on hearing and hyperacusis.

scholarly journals THE EFFECT OF FETAL CALF SERUM ON HUMAN DENTAL PULP STEM CELLS

2013 ◽  
Vol 56 (4) ◽  
pp. 142-149 ◽  
Author(s):  
Jakub Suchánek ◽  
Tereza Suchánková Kleplová ◽  
Martin Kapitán ◽  
Tomáš Soukup
Keyword(s):  
Stem Cells ◽  
Side Effects ◽  
Chromosomal Aberrations ◽  
Dental Pulp ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Dental Pulp Stem Cells ◽  
Proliferation Rate ◽  
Human Dental Pulp

Aims: Authors studied potential side effects of fetal calf serum (FCS) in cultivation media on human dental pulp stem cells (DPSC) during long term cultivation. Methods: Two lines of DPSC obtained healthy donors (male 22 years, female 23 years) were used. Both lines were cultivated under standard cultivation conditions in four different media containing 10% or 2% FCS and substituted with growth factors. During long term cultivation proliferation ability, karyotype and phenotype of DPSC were measured. Results: Both lines of DPSC cultivated in a media containing 2% FCS and ITS supplement showed the highest number of population doublings. On the other hand the proliferation rate of DPSC cultivated in a media with 2% FCS without ITS supplement was slowest. Proliferation rate of DPSC cultivated in 10% FCS media with or without FGF-2 was comparable. DPSC cultivated in a media with 10% FCS showed a significantly higher amount of chromosomal aberrations. These chromosomal aberrations do not seem to be clonal but surprisingly we found large amounts of tetraploid cells in the 9th passage in both media containing 10% FCS. Conclusions: Our study proved that cultivation of DPSC in media containing higher concentration of FCS has critical side effects on cell chromosomal stability.

Mechanisms of Therapeutic Effects of Conditioned Media from Cultured Dental Pulp Stem Cells on Diabetic Polyneuropathy

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 554-P
Author(s):  
ERIKO MAKINO ◽  
NOBUHISA NAKAMURA ◽  
MEGUMI MIYABE ◽  
MIZUHO ITO ◽  
SAKI KANADA ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Diabetic Polyneuropathy ◽  
Dental Pulp Stem Cells ◽  
Conditioned Media ◽  
Therapeutic Effects
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